BioBank CDNA - Primerdesign Ltd
... BioBank cDNA is therefore essentially free of genomic DNA and PCR inhibitors and covers the widest possible range of RNA and mRNA transcripts in the specified tissue or cell line. BioBank cDNA is useful for expression profiling of newly identified genes, and also as a positive control for the real-t ...
... BioBank cDNA is therefore essentially free of genomic DNA and PCR inhibitors and covers the widest possible range of RNA and mRNA transcripts in the specified tissue or cell line. BioBank cDNA is useful for expression profiling of newly identified genes, and also as a positive control for the real-t ...
http://www.life.umd.edu/grad/mlfsc/ DNA Bracelets
... 1) General Directions: You will need to form a group of 5 students. Remove the staple from this packet and assign one page to each student. All students will need to refer to this beginning page for the amino acid/bead conversion chart. Each student will use their assigned DNA sequence, make any req ...
... 1) General Directions: You will need to form a group of 5 students. Remove the staple from this packet and assign one page to each student. All students will need to refer to this beginning page for the amino acid/bead conversion chart. Each student will use their assigned DNA sequence, make any req ...
De Bruijn Graphs for DNA Sequencing (Part 1)
... that are complementary to substrings of length k of the fragment. Modelli Biologici Discreti ...
... that are complementary to substrings of length k of the fragment. Modelli Biologici Discreti ...
Product Information FluoroVueTM Nucleic Acid Gel Stain (10,000X
... electrophoresis the gel should be protected from light. The sensitivity of this method is slightly lower than the In-Gel Staining method. 3. Staining after electrophoresis (Post-Staining) This staining dye can be used as a post-staining method. However, the sensitivity is lower than the In-Gel Stain ...
... electrophoresis the gel should be protected from light. The sensitivity of this method is slightly lower than the In-Gel Staining method. 3. Staining after electrophoresis (Post-Staining) This staining dye can be used as a post-staining method. However, the sensitivity is lower than the In-Gel Stain ...
Complete Elimination of Endosymbiotic Algae from Paramecium
... single species or not. We have previously cloned endosymbiotic algae from P. bursaria and characterization of each clone was carried out (Nishihara et al. 1998). On the other hand, we have reported a new technique to produce algae-free paramecia by treating green paramecia with a herbicide, paraquat ...
... single species or not. We have previously cloned endosymbiotic algae from P. bursaria and characterization of each clone was carried out (Nishihara et al. 1998). On the other hand, we have reported a new technique to produce algae-free paramecia by treating green paramecia with a herbicide, paraquat ...
A Human Centromere Protein, CENP-B, Has a DNA Binding Domain
... and thrombin from human plasma from human placenta (Boehringer Mannheim GmbN). GIu-C protease hydrolyzes specifically peptide bonds at the carboxylic side of glutamic acid. Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving aromatic L-amino acids (Tyr, Phe, and Trp). Thr ...
... and thrombin from human plasma from human placenta (Boehringer Mannheim GmbN). GIu-C protease hydrolyzes specifically peptide bonds at the carboxylic side of glutamic acid. Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving aromatic L-amino acids (Tyr, Phe, and Trp). Thr ...
Differential mRNA expression levels and gene sequences of a
... gels, however, the activity co-elutes with the main peak of activity against α-naphthyl acetate. We are currently attempting to purify the MCE but are also interested in characterizing the molecular basis of the resistance at the gene level. In this paper we describe the full sequence of carboxylest ...
... gels, however, the activity co-elutes with the main peak of activity against α-naphthyl acetate. We are currently attempting to purify the MCE but are also interested in characterizing the molecular basis of the resistance at the gene level. In this paper we describe the full sequence of carboxylest ...
Automatic identification of topic boundaries in
... sequence. In some cases, indentifying such local alignments proves useful. The Smith and Waterman algorithm (Waterman 1984) finds an alignment that determines the longest/best subsequence pair that gives maximum degree of similarity between the two sequences. Single sequence alignment conveys whethe ...
... sequence. In some cases, indentifying such local alignments proves useful. The Smith and Waterman algorithm (Waterman 1984) finds an alignment that determines the longest/best subsequence pair that gives maximum degree of similarity between the two sequences. Single sequence alignment conveys whethe ...
A Mutation in the Eta Subunit of Pyruvate Dehydrogenase
... (data not shown). Comparison of the human E1 a processed gene sequence (26) with the patient-specific cDNA generated from the proband indicated that the latter was from the E1a gene on chromosome X because the codon encoding the arginine residue is CGT for the processed gene (26) in contrast to CGA ...
... (data not shown). Comparison of the human E1 a processed gene sequence (26) with the patient-specific cDNA generated from the proband indicated that the latter was from the E1a gene on chromosome X because the codon encoding the arginine residue is CGT for the processed gene (26) in contrast to CGA ...
Shelef, Katie: A Critical Analysis of Degenerate Primer Design Programs
... among organisms, and analysis of prokaryotic and microbial eukaryotic diversity, among many, many other applications. Traditional PCR allows millions of copies of the target sequence. Sequences flanking the target sequence can be used to design PCR primers, which hybridize to the flanking sequences. ...
... among organisms, and analysis of prokaryotic and microbial eukaryotic diversity, among many, many other applications. Traditional PCR allows millions of copies of the target sequence. Sequences flanking the target sequence can be used to design PCR primers, which hybridize to the flanking sequences. ...
A Study of the Asp110–Glu112 Region of EcoRII Restriction
... the pR224H plasmid. To facilitate initial selection of mutants, site-directed mutagenesis of the “Glu112” codon of the ecoRII gene was performed using the previously constructed mutant plasmid pR224HB (“Pro111” → “Ala111”), which had no BamHI restriction site. Nucleotide substitutions in the ecoRII ...
... the pR224H plasmid. To facilitate initial selection of mutants, site-directed mutagenesis of the “Glu112” codon of the ecoRII gene was performed using the previously constructed mutant plasmid pR224HB (“Pro111” → “Ala111”), which had no BamHI restriction site. Nucleotide substitutions in the ecoRII ...
Antisense derivatives of U7 small nuclear RNA as
... all types of gene therapy vectors so that they can be efficiently targeted to many different tissues and cell types. Toxic side effects have not been observed, and the desired antisense effect should only be exerted in those cells expressing the targeted pre-mRNA. Thus, in contrast to gene replaceme ...
... all types of gene therapy vectors so that they can be efficiently targeted to many different tissues and cell types. Toxic side effects have not been observed, and the desired antisense effect should only be exerted in those cells expressing the targeted pre-mRNA. Thus, in contrast to gene replaceme ...
Comparison of hair and DNA-based approaches in dietary analysis
... screened visually for unique species-specific variations. All primers were designed manually from the reference sequences, with the following criteria: 1) select amplicon size of < 250 bp to help assure amplification success of potentially degraded prey DNA present in predator feces; 2) design multi ...
... screened visually for unique species-specific variations. All primers were designed manually from the reference sequences, with the following criteria: 1) select amplicon size of < 250 bp to help assure amplification success of potentially degraded prey DNA present in predator feces; 2) design multi ...
Avian Infectious Bronchitis Virus (IBV)
... The primer and probe mix provided exploits the so-called TaqMan® principle. During PCR amplification, forward and reverse primers hybridize to the IBV cDNA. A fluorogenic probe is included in the same reaction mixture which consists of a DNA probe labeled with a 5`dye and a 3`-quencher. During PCR a ...
... The primer and probe mix provided exploits the so-called TaqMan® principle. During PCR amplification, forward and reverse primers hybridize to the IBV cDNA. A fluorogenic probe is included in the same reaction mixture which consists of a DNA probe labeled with a 5`dye and a 3`-quencher. During PCR a ...
Nucleic acid hybridisation and polymerase chain reaction
... The P C R product (amplicon) is usually visualised as a distinct band in agarose gels stained with ethidium bromide. T h e size of the amplicon, compared with molecular weight standards, c o r r e s p o n d s to the length of the target s e q u e n c e defined by the primers. The identity of the amp ...
... The P C R product (amplicon) is usually visualised as a distinct band in agarose gels stained with ethidium bromide. T h e size of the amplicon, compared with molecular weight standards, c o r r e s p o n d s to the length of the target s e q u e n c e defined by the primers. The identity of the amp ...
Binding Protein HU has a Regulatory Role in the Acid Stress
... radiations [19,20]. Besides its role in DNA compaction and protection, the HU protein has a regulatory role upon the “HU regulon,” which is composed of genes related to anaerobic growth, acid stress, high osmolarity, and the SOS response [21]. ...
... radiations [19,20]. Besides its role in DNA compaction and protection, the HU protein has a regulatory role upon the “HU regulon,” which is composed of genes related to anaerobic growth, acid stress, high osmolarity, and the SOS response [21]. ...
Caenorhabditis elegans unc-60 gene encodes
... unc-60, we employed the technique of inter-species hybridization. It has been shown that coding regions can be detected using hybridization to closely related Caenorhabditis species (Snutch 1984; Heine and Blumenthal ...
... unc-60, we employed the technique of inter-species hybridization. It has been shown that coding regions can be detected using hybridization to closely related Caenorhabditis species (Snutch 1984; Heine and Blumenthal ...
The Nterminal region of the bacterial DNA polymerase PolC features
... years ago. DnaE representatives include full-length Thermus aquaticus [5,6] and C-terminally truncated E. coli [7] PolIIIa structures, whereas PolC is represented by the structure of Geobacillus kaustophilus replicative polymerase [8]. Gram-negative and Gram-positive bacteria separated over a billio ...
... years ago. DnaE representatives include full-length Thermus aquaticus [5,6] and C-terminally truncated E. coli [7] PolIIIa structures, whereas PolC is represented by the structure of Geobacillus kaustophilus replicative polymerase [8]. Gram-negative and Gram-positive bacteria separated over a billio ...
DNA Sequencing of the eta Gene Coding for
... containing a markedly hydrophobic region in the middle. However, it is not yet known what domains in the nucleotide sequence of this gene play essential roles in coding for the biological properties of ETA. We have recently cloned the gene coding for ETB (etb) and determined the DNA sequence (unpubl ...
... containing a markedly hydrophobic region in the middle. However, it is not yet known what domains in the nucleotide sequence of this gene play essential roles in coding for the biological properties of ETA. We have recently cloned the gene coding for ETB (etb) and determined the DNA sequence (unpubl ...
DNA Pre-ConceptionStu - the Biology Scholars Program Wiki
... 4. The basic unit or monomer of DNA is composed of a(n): A. Lipid, a molecule of nitrate and one of four amino acids B. Lipid, a molecule of phosphate and one of four amino acids C. Protein, a molecule of nitrate and one of four nitrogen containing bases D. Sugar, a molecule of phosphate and one of ...
... 4. The basic unit or monomer of DNA is composed of a(n): A. Lipid, a molecule of nitrate and one of four amino acids B. Lipid, a molecule of phosphate and one of four amino acids C. Protein, a molecule of nitrate and one of four nitrogen containing bases D. Sugar, a molecule of phosphate and one of ...
Aislamiento in vitro e intensidad de infección de Rickettsia parkeri
... INTRODUCTION: Amblyomma triste (Acari: Ixodidae) is a tick distributed in the Neotropical and Nearctic regions, but most records are from South America. In Argentina, A. triste became a tick of public health relevance because of the finding of specimens infected with Rickettsia parkeri and the recen ...
... INTRODUCTION: Amblyomma triste (Acari: Ixodidae) is a tick distributed in the Neotropical and Nearctic regions, but most records are from South America. In Argentina, A. triste became a tick of public health relevance because of the finding of specimens infected with Rickettsia parkeri and the recen ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).