Additional file 4 - Springer Static Content Server
... relatives could be tested readily by targeted molecular analysis. Balanced translocations between chromosome centromeres and/or involving other repetitive chromosome heteromorphisms including 1qH, 9qH, and 16qH are likely to be the overlooked given the regions of repetitive sequences on both sides o ...
... relatives could be tested readily by targeted molecular analysis. Balanced translocations between chromosome centromeres and/or involving other repetitive chromosome heteromorphisms including 1qH, 9qH, and 16qH are likely to be the overlooked given the regions of repetitive sequences on both sides o ...
Energetics of protein–DNA interactions
... The testing set was selected from a structural classification of DNA-binding proteins (28). Because it is unclear what the maximum distance cutoff should be, for each potential, the protein–DNA specificity test was used on the training set to select an optimal distance parameter. We select a single dis ...
... The testing set was selected from a structural classification of DNA-binding proteins (28). Because it is unclear what the maximum distance cutoff should be, for each potential, the protein–DNA specificity test was used on the training set to select an optimal distance parameter. We select a single dis ...
PcrA Helicase Tightly Couples ATP Hydrolysis to Unwinding Double
... constant for this DNA junction is 1.0 s-1. This is consistent with the time limit obtained with the plasmid substrate. In practice, the biphasic nature of these traces and small signal amplitudes made full analysis difficult, in particular obtaining accurate rate information for the first phase. To ...
... constant for this DNA junction is 1.0 s-1. This is consistent with the time limit obtained with the plasmid substrate. In practice, the biphasic nature of these traces and small signal amplitudes made full analysis difficult, in particular obtaining accurate rate information for the first phase. To ...
Specific PCR Primers for the Identification of Salmonella enterica
... Salmonella is one of the most common pathogens and a major cause of foodborne diseases in human worldwide (1,2,3). Contaminated poultry products have been identified as the principal sources of Salmonella leading to foodborne illness in human(4.5). The most common serotypes of Salmonella isolated fr ...
... Salmonella is one of the most common pathogens and a major cause of foodborne diseases in human worldwide (1,2,3). Contaminated poultry products have been identified as the principal sources of Salmonella leading to foodborne illness in human(4.5). The most common serotypes of Salmonella isolated fr ...
A novel DNA modification by sulphur
... Methylation is the most common covalent modification of DNA so far reported (Luria and Human, 1952; Pradhan et al., 1999), although some bacteriophages modify their DNA in other ways (Hattman, 1979; 1980; Swinton et al., 1983). An unusual and seemingly novel DNA modification was discovered in the Gr ...
... Methylation is the most common covalent modification of DNA so far reported (Luria and Human, 1952; Pradhan et al., 1999), although some bacteriophages modify their DNA in other ways (Hattman, 1979; 1980; Swinton et al., 1983). An unusual and seemingly novel DNA modification was discovered in the Gr ...
Phenotypic and Genotypic Comparisons among Strains of the
... (Oxoid), and 0.2% yeast extract (Oxoid) dissolved in distilled water (wt/vol) at 37°C for 15 h. The cells were harvested and lysed, and the nucleic acids were extracted and purified by the method of Marmur (16), with two modifications: immediately after adding sodium lauryl sulfate, the lysozymetrea ...
... (Oxoid), and 0.2% yeast extract (Oxoid) dissolved in distilled water (wt/vol) at 37°C for 15 h. The cells were harvested and lysed, and the nucleic acids were extracted and purified by the method of Marmur (16), with two modifications: immediately after adding sodium lauryl sulfate, the lysozymetrea ...
Effects of Salt Concentrations and Bending Energy on the Extent of
... therefore a measure of the stressed state of the DNA, due to both its crowding and bending. It has recently been shown that this force can be determined by experiments in which osmotic pressure is employed to inhibit the ejection (6). The results of these studies are consistent with the predictions ...
... therefore a measure of the stressed state of the DNA, due to both its crowding and bending. It has recently been shown that this force can be determined by experiments in which osmotic pressure is employed to inhibit the ejection (6). The results of these studies are consistent with the predictions ...
PTC Genetics Lab Student Worksheet
... receptor cells that detect sweet taste. The stimulated receptor cells send nerve impulses to gustatory region of the brain where the sense of taste is interpreted. Different types of taste receptors are activated by different chemicals, and the nerve impulses they send to the brain are interpreted a ...
... receptor cells that detect sweet taste. The stimulated receptor cells send nerve impulses to gustatory region of the brain where the sense of taste is interpreted. Different types of taste receptors are activated by different chemicals, and the nerve impulses they send to the brain are interpreted a ...
Parasitoids, predators and PCR: the use of diagnostic
... classification of the specimen. As universal primers are used in the initial PCR assay, barcoding can identify new or previously unknown species, cryptic species or strains. However, as a single character (i.e., one portion of a gene) is used in the identification of a specimen, this technique may hav ...
... classification of the specimen. As universal primers are used in the initial PCR assay, barcoding can identify new or previously unknown species, cryptic species or strains. However, as a single character (i.e., one portion of a gene) is used in the identification of a specimen, this technique may hav ...
Detection of genetically modified plants
... It must be sensitive and reliable enough to obtain exact results in all control laboratories. ...
... It must be sensitive and reliable enough to obtain exact results in all control laboratories. ...
msc_botnay_pre_pap1_bl2
... modifications to bring about decondensation of chromatin, to allow access of DNA replication or transcription machinery to naked DNA. These modifications include ubiquitination, acetylation, methylation and phosphorylation of some specific amino acid residues of histones. Acetylation and methylation ...
... modifications to bring about decondensation of chromatin, to allow access of DNA replication or transcription machinery to naked DNA. These modifications include ubiquitination, acetylation, methylation and phosphorylation of some specific amino acid residues of histones. Acetylation and methylation ...
A helicoidal transfer matrix model for inhomogeneous DNA melting
... models are mostly used to obtain a more fundamental, sequence independent, physical understanding of the DNA melting phenomenon, such as the order of the phase transition, the existence of nonlinear ‘bubble’ excitations, etc. (see [19] for a recent review paper). Moreover, although both types of mod ...
... models are mostly used to obtain a more fundamental, sequence independent, physical understanding of the DNA melting phenomenon, such as the order of the phase transition, the existence of nonlinear ‘bubble’ excitations, etc. (see [19] for a recent review paper). Moreover, although both types of mod ...
rodrigo_brindeirox
... – Syntheny between mutations multi-resistant virus or – Mutations in different subpopulations mixture of resistant and wild type viruses. ...
... – Syntheny between mutations multi-resistant virus or – Mutations in different subpopulations mixture of resistant and wild type viruses. ...
1. If the inside ends
... 1. Replicative transposase cuts only one strand at the junction. 2. Nonreplicative transposase makes cuts in both strands in the junction. • The similarity between replicative and nonreplicative transposition 1. The cut 5’ ends of the target DNA are joined to the free 3’ ends of the transposon. 2. T ...
... 1. Replicative transposase cuts only one strand at the junction. 2. Nonreplicative transposase makes cuts in both strands in the junction. • The similarity between replicative and nonreplicative transposition 1. The cut 5’ ends of the target DNA are joined to the free 3’ ends of the transposon. 2. T ...
Altering substrate specificity of catechol 2,3
... site underlined) purchased from IBB PAN (Warsaw, Poland). Primers were designed based on the regions of the catechol 2,3-dioxygenases with high sequence homology, and allowing amplification of C23O gene from start to stop codons (Hupert-Kocurek et al., 2012). PCR reaction was performed in 25 µL volu ...
... site underlined) purchased from IBB PAN (Warsaw, Poland). Primers were designed based on the regions of the catechol 2,3-dioxygenases with high sequence homology, and allowing amplification of C23O gene from start to stop codons (Hupert-Kocurek et al., 2012). PCR reaction was performed in 25 µL volu ...
Full-Size Color Slides
... High Tau Expression is Associated With Residual Disease After Chemotherapy Rouzier et al. PNAS 2005;11:5678-85 ...
... High Tau Expression is Associated With Residual Disease After Chemotherapy Rouzier et al. PNAS 2005;11:5678-85 ...
Nitrosation of aspartic acid, aspartame, and glycine ethylester
... taken into account. For this purpose, the following data bad to be collected. Molar extinction coefficient of alkylated NBP When NBP was incubated with a large excess of either methyl iodide or iodoacetic acid in acetone and aliquots were taken at different times, a plateau was reached after about 3 ...
... taken into account. For this purpose, the following data bad to be collected. Molar extinction coefficient of alkylated NBP When NBP was incubated with a large excess of either methyl iodide or iodoacetic acid in acetone and aliquots were taken at different times, a plateau was reached after about 3 ...
Ezekiel Code with DNA Molecule: Fifteen Similarities
... 13A: Ezekiel described: “when the creatures moved, I heard the sound of their wings, like the roar of rushing waters”. 13B: According to the biological books, the “space” inside of cell under the membrane called cytoplasm. The entire cell including nucleus is full of water and also the enzyme-cataly ...
... 13A: Ezekiel described: “when the creatures moved, I heard the sound of their wings, like the roar of rushing waters”. 13B: According to the biological books, the “space” inside of cell under the membrane called cytoplasm. The entire cell including nucleus is full of water and also the enzyme-cataly ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).