Supplementary Information 410 475
... Comparison of vertebrate TSG amino acid sequences. (A) Alignment of TSG amino acid sequences for human (hTSG), mouse (mTSG), chick (cTSG), Xenopus (xTSG), and zebrafish (zTSG) with Drosophila TSG (dTSG) amino acid sequences6 and those of two recently reported gene products of the Drosophila genome ( ...
... Comparison of vertebrate TSG amino acid sequences. (A) Alignment of TSG amino acid sequences for human (hTSG), mouse (mTSG), chick (cTSG), Xenopus (xTSG), and zebrafish (zTSG) with Drosophila TSG (dTSG) amino acid sequences6 and those of two recently reported gene products of the Drosophila genome ( ...
Leishmania donovani - Oxford Academic
... boiling with 6 M HCl. After removal of HCl, the dried hydrolysates were resuspended in 10 µl of H2O containing 200 nmol each of phosphoserine, phoshphothreonine and phosphotyrosine. A few microliters of the samples were spotted on the silica gel plate. Phosphoamino acid mix markers were also spotted ...
... boiling with 6 M HCl. After removal of HCl, the dried hydrolysates were resuspended in 10 µl of H2O containing 200 nmol each of phosphoserine, phoshphothreonine and phosphotyrosine. A few microliters of the samples were spotted on the silica gel plate. Phosphoamino acid mix markers were also spotted ...
JOIN2004 Universidade do Minho
... Regular expressions permit you to find and alter many patterns with relative ease. The excellent regular expressions in Perl are a major reason for Perl's success as a bioinformatics programming language. ...
... Regular expressions permit you to find and alter many patterns with relative ease. The excellent regular expressions in Perl are a major reason for Perl's success as a bioinformatics programming language. ...
BSC1005 /Belk_Chapter 7
... 12.10 DNA Fingerprinting 1st-The DNA molecule is cut with restriction enzymes 2nd- we have to separate the fragments This is done by a technique called gel electrophoresis The DNA is placed on a tray filled with gel through which an electric current runs causing the fragments to move through the ge ...
... 12.10 DNA Fingerprinting 1st-The DNA molecule is cut with restriction enzymes 2nd- we have to separate the fragments This is done by a technique called gel electrophoresis The DNA is placed on a tray filled with gel through which an electric current runs causing the fragments to move through the ge ...
Water SA
... double-stranded DNA. Therefore, it will not only bind to PCR products but to non-specific products that have been amplified, as well as primer dimers. Due to this non-specificity, SYBR Green requires more time for optimisation than protocols based on primers and probes. Nevertheless, when SYBR Gree ...
... double-stranded DNA. Therefore, it will not only bind to PCR products but to non-specific products that have been amplified, as well as primer dimers. Due to this non-specificity, SYBR Green requires more time for optimisation than protocols based on primers and probes. Nevertheless, when SYBR Gree ...
Lack of biological significance in the `linguistic features` of
... Zipf analyses of both natural and associated artificial sequences (for, say, Di = 200 bp), using 6-tuples as ‘words’ produced essentially indistinguishable graphs. We obtained essentially the same ‘negative’ result for many different natural DNAs, among others: (i) the human HSRETBLAS (cf. above), a ...
... Zipf analyses of both natural and associated artificial sequences (for, say, Di = 200 bp), using 6-tuples as ‘words’ produced essentially indistinguishable graphs. We obtained essentially the same ‘negative’ result for many different natural DNAs, among others: (i) the human HSRETBLAS (cf. above), a ...
IS1245 restriction fragment length polymorphism typing - HAL
... a probe for hybridization prepared by PCR amplification on an IS1245 DNA-containing plasmid and higher-stringency washing conditions after hybridization. Except for the strains with the three-band pattern of birds, IS1245 RFLP patterns of M. avium isolates consist of a high average number of bands, ...
... a probe for hybridization prepared by PCR amplification on an IS1245 DNA-containing plasmid and higher-stringency washing conditions after hybridization. Except for the strains with the three-band pattern of birds, IS1245 RFLP patterns of M. avium isolates consist of a high average number of bands, ...
CHAPTER ONE INTRODUCTION 1
... 2004). However, an integrated transgene can be silenced following failure at the transcription level caused by DNA-methylation commonly induced by promoter homology (Park et al., 1996; Stam et al., 1997). Similarly, silencing can be post-transcriptional where transcription occurs at a low level or i ...
... 2004). However, an integrated transgene can be silenced following failure at the transcription level caused by DNA-methylation commonly induced by promoter homology (Park et al., 1996; Stam et al., 1997). Similarly, silencing can be post-transcriptional where transcription occurs at a low level or i ...
Rapid Publication - Journal of Clinical Investigation
... blunt-ended with T4 polymerase, and ligated to synthetic EcoRI linkers after methylation of internal EcoRI sites. After it was digested with EcoRI, cDNA was purified from free linkers by Sepharose CL4-B chromatography and ligated into EcoRI-digested alkaline phosphatasetreated lambda gtl I DNA (10). ...
... blunt-ended with T4 polymerase, and ligated to synthetic EcoRI linkers after methylation of internal EcoRI sites. After it was digested with EcoRI, cDNA was purified from free linkers by Sepharose CL4-B chromatography and ligated into EcoRI-digested alkaline phosphatasetreated lambda gtl I DNA (10). ...
DNA polymerase active site is highly mutable
... NA polymerases are responsible for the replication and maintenance of the genome, a role that is central to accurately transmitting genetic information from generation to generation. DNA polymerases have an active site architecture that specifically configures to and incorporates each of the four de ...
... NA polymerases are responsible for the replication and maintenance of the genome, a role that is central to accurately transmitting genetic information from generation to generation. DNA polymerases have an active site architecture that specifically configures to and incorporates each of the four de ...
Hypercholesterolemia
... To detect the FH mutation, DNA is obtained from the patient’s blood or saliva; the section of DNA containing the LDL receptor gene is then amplified by the polymerase chain reaction (PCR). The amplified DNA is analyzed to see if there is a mutation. To analyze the DNA, investigators use restriction en ...
... To detect the FH mutation, DNA is obtained from the patient’s blood or saliva; the section of DNA containing the LDL receptor gene is then amplified by the polymerase chain reaction (PCR). The amplified DNA is analyzed to see if there is a mutation. To analyze the DNA, investigators use restriction en ...
Molecular markers in Brassica Rapa
... (BRAD gene ID: Bra031539) from the putative promoter region to the 500 bp downstream region from the stop codon (Supplementary Table 1). PCR conditions were as follows: 94 °C for 5 min; followed by cycles of 94 °C for 1 min, 58 °C for 1 min, and 72 °C for 1 min; and final extension at 72 °C for 7 mi ...
... (BRAD gene ID: Bra031539) from the putative promoter region to the 500 bp downstream region from the stop codon (Supplementary Table 1). PCR conditions were as follows: 94 °C for 5 min; followed by cycles of 94 °C for 1 min, 58 °C for 1 min, and 72 °C for 1 min; and final extension at 72 °C for 7 mi ...
Ribosome Display: In Vitro Selection of Protein
... vector containing the necessary flanking regions (ribosome-display vector, pRDV; Fig. 2) . The library is PCR amplified, cut with the appropriate restriction enzymes, and ligated into the vector such that it is in frame with the spacer (Fig. 3). A second PCR on this ligation product directly amplifi ...
... vector containing the necessary flanking regions (ribosome-display vector, pRDV; Fig. 2) . The library is PCR amplified, cut with the appropriate restriction enzymes, and ligated into the vector such that it is in frame with the spacer (Fig. 3). A second PCR on this ligation product directly amplifi ...
Bacterial community composition in the rhizosphere of a transgenic
... can vary dramatically depending on the chromosomal insertion site [17] and other genes, e.g. those in proximity to the insertion site, may also be a¡ected. Should such unintended e¡ects have any impact on soil microbial activity, it would likely be seen ¢rst in the rhizosphere. The composition of mi ...
... can vary dramatically depending on the chromosomal insertion site [17] and other genes, e.g. those in proximity to the insertion site, may also be a¡ected. Should such unintended e¡ects have any impact on soil microbial activity, it would likely be seen ¢rst in the rhizosphere. The composition of mi ...
Applied and Environmental Microbiology
... cereus and B. mycoides, which belong to the neutral metalloprotease class, play an important role in proteolytic processes in soils (6, 16–18). The DNA sequence of the B. cereus thermolysin-like enzyme has a high degree of homology to the sequences of other metalloproteases, especially those from B. ...
... cereus and B. mycoides, which belong to the neutral metalloprotease class, play an important role in proteolytic processes in soils (6, 16–18). The DNA sequence of the B. cereus thermolysin-like enzyme has a high degree of homology to the sequences of other metalloproteases, especially those from B. ...
Structure and evolution of Apetala3, a sex
... for investigations into the evolution of sex chromosomes in plants. Silene latifolia Poiret (syn. Melandrium album Garcke, syn. Melandrium pratense Roehl.) is a strictly dioecious, perennial herb of the Caryophyllaceae family. The sex of individual plants is genetically determined by sex chromosomes ...
... for investigations into the evolution of sex chromosomes in plants. Silene latifolia Poiret (syn. Melandrium album Garcke, syn. Melandrium pratense Roehl.) is a strictly dioecious, perennial herb of the Caryophyllaceae family. The sex of individual plants is genetically determined by sex chromosomes ...
DNA - CS.Duke
... The code in the class DNABenchMark can be used to benchmark the cutAndSplice method. The code given to you will pop-‐up a file-‐dialog box — when run you can use this to nav ...
... The code in the class DNABenchMark can be used to benchmark the cutAndSplice method. The code given to you will pop-‐up a file-‐dialog box — when run you can use this to nav ...
Biology 343 Lab (Dorn, Shade)
... designed to give you hands-on experience with modern genetics techniques. It is not designed to coincide very much with your lecture material; in fact, it could be a course of its own, which is what may happen to it someday. You will be learning molecular lab techniques that are useful for genetics, ...
... designed to give you hands-on experience with modern genetics techniques. It is not designed to coincide very much with your lecture material; in fact, it could be a course of its own, which is what may happen to it someday. You will be learning molecular lab techniques that are useful for genetics, ...
MOLECULAR ANALYSIS OF CYSTIC FIBROSIS PATIENTS IN
... Methods: Depending on the preceding analysis, four different mutation detection methods were used. A commercial assay targeting the most common CF-causing mutations was performed as the first test followed by an allele specific PCR for CFTRdele2,3(21kb), Sanger sequencing and MLPA analysis of the co ...
... Methods: Depending on the preceding analysis, four different mutation detection methods were used. A commercial assay targeting the most common CF-causing mutations was performed as the first test followed by an allele specific PCR for CFTRdele2,3(21kb), Sanger sequencing and MLPA analysis of the co ...
Presentation
... Popular and classic DNA damage assays • Neutral and alkaline elution through filters or separation on sucrose gradients - a classic assay for DSB and SSB+DSB, respectively • Comet assay - sensitive assay for SSB that can be used for single cells; less sensitive (10Gy) for DSBs H2AX focus formatio ...
... Popular and classic DNA damage assays • Neutral and alkaline elution through filters or separation on sucrose gradients - a classic assay for DSB and SSB+DSB, respectively • Comet assay - sensitive assay for SSB that can be used for single cells; less sensitive (10Gy) for DSBs H2AX focus formatio ...
Modified PDF
... was severely impaired (van Breukelen et al. 2000). Still DBP could bind DNA efficiently and cooperatively. This suggests that flexibility of the protein chain is an essential prerequisite for DNA chain elongation. One explanation for the need of flexibility is that, when bound in the replication for ...
... was severely impaired (van Breukelen et al. 2000). Still DBP could bind DNA efficiently and cooperatively. This suggests that flexibility of the protein chain is an essential prerequisite for DNA chain elongation. One explanation for the need of flexibility is that, when bound in the replication for ...
Comparisons of Maize pericarp color1 Alleles
... (CGCC) (Figure 6B). In summary, the P1-rw1077 allele contains a 6.9-kb segment of the p2 coding sequence that replaces most of the p1 coding sequence. The resulting gene is expected to generate transcripts with p1-like sequences in the 59 UTR and p2-like sequences in the remaining coding region. The ...
... (CGCC) (Figure 6B). In summary, the P1-rw1077 allele contains a 6.9-kb segment of the p2 coding sequence that replaces most of the p1 coding sequence. The resulting gene is expected to generate transcripts with p1-like sequences in the 59 UTR and p2-like sequences in the remaining coding region. The ...
Developmental Validation of the Quantifiler Real-Time
... PCR technology for the detection of target sequence amplification (11). This system is based on a set of two oligonucleotide PCR primers specific for the target DNA sequence, or amplicon, plus ...
... PCR technology for the detection of target sequence amplification (11). This system is based on a set of two oligonucleotide PCR primers specific for the target DNA sequence, or amplicon, plus ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).