Abstract
... was extracted by phenol:chloroform separation in the presence of CTAB detergent and by DNeasy. Plasmid extractions were performed with the Qiagen mini-prep kit and the Wizard mini-prep kit. These DNAs were used in Southern hybridization experiments with probes for class A (TEM1-type) and class B ...
... was extracted by phenol:chloroform separation in the presence of CTAB detergent and by DNeasy. Plasmid extractions were performed with the Qiagen mini-prep kit and the Wizard mini-prep kit. These DNAs were used in Southern hybridization experiments with probes for class A (TEM1-type) and class B ...
slides - ARUP.utah.edu - The University of Utah
... May uncover copy number changes of unclear clinical significance Will not detect copy number changes in regions of the genome that are not on the array platform (chip) ...
... May uncover copy number changes of unclear clinical significance Will not detect copy number changes in regions of the genome that are not on the array platform (chip) ...
Biol207 Final Exam
... diagram of a 9 kbp EcoR I (E) restriction fragment subcloned from a cosmid clone that he cloned from his Yeast genomic library. The yeast genome is ~13 Mbp long. The restriction map for this fragment shows the Xba I sites (X) and BamH I sites (B). The locations of the only three genes (A, B, and C) ...
... diagram of a 9 kbp EcoR I (E) restriction fragment subcloned from a cosmid clone that he cloned from his Yeast genomic library. The yeast genome is ~13 Mbp long. The restriction map for this fragment shows the Xba I sites (X) and BamH I sites (B). The locations of the only three genes (A, B, and C) ...
EXPRESSED SEQUENCE TAGS FROM IMMUNE TISSUES OF
... virus (VHSV) are two pathogens that affect olive flounder culture causing serious economic losses to the olive flounder industry. Little is known about the molecular mechanisms for disease resistance and host pathogen interactions in this species. In this study, tens of thousands of expressed sequen ...
... virus (VHSV) are two pathogens that affect olive flounder culture causing serious economic losses to the olive flounder industry. Little is known about the molecular mechanisms for disease resistance and host pathogen interactions in this species. In this study, tens of thousands of expressed sequen ...
Multiplex STR Analysis by Capillary Electrophoresis
... Most short tandem repeat loci used in identity testing have repeats that are four base pairs in length, with allele sizes between 100bp and 300bp. Table 1 lists some of the commonly used STRs. The relatively small size of STR alleles reduces the effects of preferential amplification. Thus, more high ...
... Most short tandem repeat loci used in identity testing have repeats that are four base pairs in length, with allele sizes between 100bp and 300bp. Table 1 lists some of the commonly used STRs. The relatively small size of STR alleles reduces the effects of preferential amplification. Thus, more high ...
Slide 1
... they used that same probe to screen Genomic Libraries from healthy and ataxic people, as well as to screen a cDNA Library (made from fetal brain ...
... they used that same probe to screen Genomic Libraries from healthy and ataxic people, as well as to screen a cDNA Library (made from fetal brain ...
2015 CPT Changes Pathology and Laboratory Services
... 80374 Stereoisomer (enantiomer) analysis, single drug class 80375 Drug(s) or substance(s), definitive, qualitative or quantitative, not otherwise specified; 1-3 80376 Drug(s) or substance(s), definitive, qualitative or quantitative, not otherwise specified; 4-6 80377 Drug(s) or substance(s), definit ...
... 80374 Stereoisomer (enantiomer) analysis, single drug class 80375 Drug(s) or substance(s), definitive, qualitative or quantitative, not otherwise specified; 1-3 80376 Drug(s) or substance(s), definitive, qualitative or quantitative, not otherwise specified; 4-6 80377 Drug(s) or substance(s), definit ...
Non-small-cell lung carcinoma
... some CNVs have been associated with susceptibility or resistance to disease Gene copy number can be elevated in cancer cells. the EGFR copy number can be higher than normal in non-small cell lung cancer ...
... some CNVs have been associated with susceptibility or resistance to disease Gene copy number can be elevated in cancer cells. the EGFR copy number can be higher than normal in non-small cell lung cancer ...
Bioinformatics Overview, NCBI & GenBank
... and are not usually assembled into contigs. They are lowquality sequences that are often used to check whether another center is already sequencing a particular clone. • Phase 1: Entries are assembled into contigs that are separated by sequence gaps, the relative order and orientation of which are n ...
... and are not usually assembled into contigs. They are lowquality sequences that are often used to check whether another center is already sequencing a particular clone. • Phase 1: Entries are assembled into contigs that are separated by sequence gaps, the relative order and orientation of which are n ...
- Horizon Discovery
... Fixation using formalin is a critical step in the preparation of histological sections. It ensures the preservation of tissue architecture and cell morphology by cross-linking biomolecules. If fixation is not carried out under optimal conditions a tissue specimen can be irreversibly damaged. Methods ...
... Fixation using formalin is a critical step in the preparation of histological sections. It ensures the preservation of tissue architecture and cell morphology by cross-linking biomolecules. If fixation is not carried out under optimal conditions a tissue specimen can be irreversibly damaged. Methods ...
Co-dominant SCAR marker, P6-25 - Department of Plant Pathology
... Ty-3 locus associated with lines derived from S. chilense LA2779, respectively. The 650-bp fragment from Gc171 had one large insert, when compared with the S. lycopersicum sequence. Conclusions: This set of primers detect co-dominant SCAR marker, P6-25, for the ty-3, Ty-3 and Ty-3a alleles at the FE ...
... Ty-3 locus associated with lines derived from S. chilense LA2779, respectively. The 650-bp fragment from Gc171 had one large insert, when compared with the S. lycopersicum sequence. Conclusions: This set of primers detect co-dominant SCAR marker, P6-25, for the ty-3, Ty-3 and Ty-3a alleles at the FE ...
Brief introduction to whole-genome selection in cattle using single
... The current cost to researchers for one 50K SNP chip plus analysis is approximately US$200; smaller chips could cost as little as US$20–50. Definition of genomic selection Whole-genome selection (or genomic selection) may be defined as using genotypes defined by a set of SNPs to select for optimal p ...
... The current cost to researchers for one 50K SNP chip plus analysis is approximately US$200; smaller chips could cost as little as US$20–50. Definition of genomic selection Whole-genome selection (or genomic selection) may be defined as using genotypes defined by a set of SNPs to select for optimal p ...
Genetic Polymorphism and SNPs - McGill School Of Computer
... Haplotype (haploid genotype) A particular pattern of sequential SNPs (or alleles) found on a single chromosome. These SNPs tend to be inherited together over time and can serve as disease- gene markers. The examination of single chromosome sets (haploid sets), as opposed to the usual chromosome pair ...
... Haplotype (haploid genotype) A particular pattern of sequential SNPs (or alleles) found on a single chromosome. These SNPs tend to be inherited together over time and can serve as disease- gene markers. The examination of single chromosome sets (haploid sets), as opposed to the usual chromosome pair ...
Recombinant DNA Technology and Molecular Cloning
... • If a match is found with the protein under study, the EST provides the unique DNA sequence of that portion of cDNA. • A probe can then be synthesized and used to screen a library for the entire cDNA or genomic clone. ...
... • If a match is found with the protein under study, the EST provides the unique DNA sequence of that portion of cDNA. • A probe can then be synthesized and used to screen a library for the entire cDNA or genomic clone. ...
FTv6_6_changes
... between the indicated points). From October 2006 the usage of this descriptor is restricted: it is illegal to use "a single base from a range" (c) either on its own or in combination with the "sequence span" (d) descriptor for newly created entries. The existing entries where such combinations exist ...
... between the indicated points). From October 2006 the usage of this descriptor is restricted: it is illegal to use "a single base from a range" (c) either on its own or in combination with the "sequence span" (d) descriptor for newly created entries. The existing entries where such combinations exist ...
SNP discovery
... SNPs are now the marker of choice • SNPs are “abundant” across the genome genomic DNA • Large “pools of SNPs” can be used to identify sets of polymorphic markers • SNP markers are “bi-allelic” making allele calling more simple • SNP data from different systems or groups can be “easily merged in a d ...
... SNPs are now the marker of choice • SNPs are “abundant” across the genome genomic DNA • Large “pools of SNPs” can be used to identify sets of polymorphic markers • SNP markers are “bi-allelic” making allele calling more simple • SNP data from different systems or groups can be “easily merged in a d ...
T - Crime Scene
... Several loci along our DNA have been identified as possessing STRs (thanks in part to the Human Genome Project), and the DNA profiling community has selected 13 regions for identity analysis These 13 loci ALL contain 4 nucleotide (tetrameric) repeats Through population studies, the numbers and types ...
... Several loci along our DNA have been identified as possessing STRs (thanks in part to the Human Genome Project), and the DNA profiling community has selected 13 regions for identity analysis These 13 loci ALL contain 4 nucleotide (tetrameric) repeats Through population studies, the numbers and types ...
Slide 1
... monogenic diseases (e.g., cystic fibrosis) nsSNPs are frequent (>1%) and can modify risks of major common (multigenic, complex) diseases (e.g., cancer, cardiovascular disease, mental illness, autoimmune states, diabetes) In some cases, however, it is difficult to make a distinction ...
... monogenic diseases (e.g., cystic fibrosis) nsSNPs are frequent (>1%) and can modify risks of major common (multigenic, complex) diseases (e.g., cancer, cardiovascular disease, mental illness, autoimmune states, diabetes) In some cases, however, it is difficult to make a distinction ...
Identification of genes altered in a mos1 mutagenesis I
... I-PCR Protocol from Bessereau; [email protected] /2002; PCR cloning protocol from stratagene lab manual; modifications for class by V. Praitis. Day 1: Worm lysis I-PCR can be performed on a worm lysate or on purified genomic DNA. Worm lysis works fine most of the time. However, for reasons that we ...
... I-PCR Protocol from Bessereau; [email protected] /2002; PCR cloning protocol from stratagene lab manual; modifications for class by V. Praitis. Day 1: Worm lysis I-PCR can be performed on a worm lysate or on purified genomic DNA. Worm lysis works fine most of the time. However, for reasons that we ...
Sigma Xi, Montreal Nov 2004 - Biology Department | UNC Chapel Hill
... the transcriptional regulation of those genes and thus contribute to phenotypic variation. However, we do not know how frequently such variations in gene location occur among individuals within populations. Additionally, we do not know the degree to which such differences in chromosomal location aff ...
... the transcriptional regulation of those genes and thus contribute to phenotypic variation. However, we do not know how frequently such variations in gene location occur among individuals within populations. Additionally, we do not know the degree to which such differences in chromosomal location aff ...
sequencing all mRNAs
... + much higher resolution - up to nucleotide level + location information +/- Sampled signal (more on next slides) ...
... + much higher resolution - up to nucleotide level + location information +/- Sampled signal (more on next slides) ...
Structural analysis of both products of a reciprocal translocation
... fragment 1n the cloned DNA that spans the J5 rearrangement s i t e , clearly demonstrating that the rearrangement found In cloned BL22 DNA 1s not a cloning a r t i f a c t . A schematic diagram of the reciprocal rearranged fragment and germline c-myc DNA 1s shown below the autoradiogram. The positio ...
... fragment 1n the cloned DNA that spans the J5 rearrangement s i t e , clearly demonstrating that the rearrangement found In cloned BL22 DNA 1s not a cloning a r t i f a c t . A schematic diagram of the reciprocal rearranged fragment and germline c-myc DNA 1s shown below the autoradiogram. The positio ...
Molecular Inversion Probe
Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping as well as for studying gene copy alterationsand characteristics of specific genomic loci to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.