![HiPer® Real-Time PCR Teaching Kit](http://s1.studyres.com/store/data/023420735_1-0c85cc812a3fd2168bced32d7f5057dd-300x300.png)
VCS: Tool for Visualizing Copy Number Variation and Single Nucleotide Polymorphism
... values for different research purposes. In addition, user can draw a Manhattan plot which easily can define appropriate significance value, and can perform the comparison among samples selected in this menu. The input file needs matrix format data with the information of physical location and the va ...
... values for different research purposes. In addition, user can draw a Manhattan plot which easily can define appropriate significance value, and can perform the comparison among samples selected in this menu. The input file needs matrix format data with the information of physical location and the va ...
Designing synthetic MLPA probes - MRC
... NG_sequences also include intronic sequences. Nucleotide. The combination of LPO and RPO. DNA oligonucleotide which, when annealed to a complementary DNA sequence, can be used as starting point for extension by a polymerase enzyme during PCR. Note that in MLPA, a primer is not the same as a probe! A ...
... NG_sequences also include intronic sequences. Nucleotide. The combination of LPO and RPO. DNA oligonucleotide which, when annealed to a complementary DNA sequence, can be used as starting point for extension by a polymerase enzyme during PCR. Note that in MLPA, a primer is not the same as a probe! A ...
Nyholt and colleagues provided compelling evidence for the
... (DXS1001-DXS1206, DXS984-DXS1123 and DXS8091-qter). Using several sequence-analysis tools and database-mining procedures, we determined that these intervals contain at least 100 genes. We selected 19 candidate genes, on the basis of their potential functional significance or physical position: GRIA3 ...
... (DXS1001-DXS1206, DXS984-DXS1123 and DXS8091-qter). Using several sequence-analysis tools and database-mining procedures, we determined that these intervals contain at least 100 genes. We selected 19 candidate genes, on the basis of their potential functional significance or physical position: GRIA3 ...
In vivo target function
... • Role in platelet aggregration (genetic analysis and in vitro assay) ...
... • Role in platelet aggregration (genetic analysis and in vitro assay) ...
Large-scale association studies
... • For meta-analysis, need to impute to the same set of SNPs before analysis – most people us 2.5 million HapMap Phase II SNPs – starting to use 38 million 1000 Genomes SNPs – for additive genetic model, doesn’t matter whether SNPs are measured or imputed. – slightly more work needed for non-additive ...
... • For meta-analysis, need to impute to the same set of SNPs before analysis – most people us 2.5 million HapMap Phase II SNPs – starting to use 38 million 1000 Genomes SNPs – for additive genetic model, doesn’t matter whether SNPs are measured or imputed. – slightly more work needed for non-additive ...
SNP Array Activity Learning Objectives Introduction
... DNA microarrays (also called DNA arrays and gene chips) are manufactured by placing many singlestranded DNA molecules with a single known sequence in a single spot on a glass plate or slide. Many different sequences may be included in a single microarray, with each sequence being assigned to its own ...
... DNA microarrays (also called DNA arrays and gene chips) are manufactured by placing many singlestranded DNA molecules with a single known sequence in a single spot on a glass plate or slide. Many different sequences may be included in a single microarray, with each sequence being assigned to its own ...
Case Report Section
... The patient described here is a 17 year old female presented with upper respiratory tract infection and bruises for 2 weeks. Subsequently she was diagnosed with AML (FAB M4 eos). Cytogenetics, performed on bone marrow aspirate revealed a unique structural abnormality of chromosome 16 which was inter ...
... The patient described here is a 17 year old female presented with upper respiratory tract infection and bruises for 2 weeks. Subsequently she was diagnosed with AML (FAB M4 eos). Cytogenetics, performed on bone marrow aspirate revealed a unique structural abnormality of chromosome 16 which was inter ...
SuperScript™ III Platinum® One-Step Quantitative RT
... Note that some TaqMan probes utilize a TAMRA quencher, which can be used as a reference dye for normalization of data. This technique is only valid for a reaction containing a single TaqMan probe, and should not be used in multiplex applications. ...
... Note that some TaqMan probes utilize a TAMRA quencher, which can be used as a reference dye for normalization of data. This technique is only valid for a reaction containing a single TaqMan probe, and should not be used in multiplex applications. ...
Hy-Line - LGC Group
... Purified amplicons (exons 2-6) were sequenced by SeqWright, and sequence electopherograms analysed by sequencher 4.9 (Gene Codes Corp.). Sequences were aligned with the Jungle Fowl (JF) genome sequences accessed from UCSC. SNP detection and genotyping Initially, SNPs were detected by PCR amplificati ...
... Purified amplicons (exons 2-6) were sequenced by SeqWright, and sequence electopherograms analysed by sequencher 4.9 (Gene Codes Corp.). Sequences were aligned with the Jungle Fowl (JF) genome sequences accessed from UCSC. SNP detection and genotyping Initially, SNPs were detected by PCR amplificati ...
A novel human cytochrome P4S0 gene (P450IIB): chromosomal
... We have isolated from a single human liver cDNA library two clones which are highly homologous (78% over the coding region) to the major phenobarbital-inducible P450 from rat (P450IIB1) . This is the first direct demonstration of the presence of the P450IIB gene subfamily in humans. This subfamily i ...
... We have isolated from a single human liver cDNA library two clones which are highly homologous (78% over the coding region) to the major phenobarbital-inducible P450 from rat (P450IIB1) . This is the first direct demonstration of the presence of the P450IIB gene subfamily in humans. This subfamily i ...
Escherichia coli
... coli-spp copy number / CT value. Alternatively the positive control can be used at a single dilution where full quantitative analysis of the samples is not required. Each time the kit is used, at least one positive control reaction must be included in the run. A positive result indicates that the pr ...
... coli-spp copy number / CT value. Alternatively the positive control can be used at a single dilution where full quantitative analysis of the samples is not required. Each time the kit is used, at least one positive control reaction must be included in the run. A positive result indicates that the pr ...
Terauchi, R., Abe, A., Takagi, H., Tamiru, M
... markers” to test their association with the phenotype. Following identification of genetic markers that show association with a phenotype, we explore their vicinity to identify the very genetic change that is responsible for the phenotypic variation. Two major approaches have been largely employed i ...
... markers” to test their association with the phenotype. Following identification of genetic markers that show association with a phenotype, we explore their vicinity to identify the very genetic change that is responsible for the phenotypic variation. Two major approaches have been largely employed i ...
Lecture#29 - RFLP-2 - Locating Genes in Large Genomes Using
... - probe has to be unique (not repeated DNA sequences) - try many different restriction-enzyme/probe combinations - any randomly chosen unique DNA probe can usually serve as an RFLP marker. 2. RFLP analysis requires a small amount of DNA. - a blood sample is usually enough to do many tests - can cult ...
... - probe has to be unique (not repeated DNA sequences) - try many different restriction-enzyme/probe combinations - any randomly chosen unique DNA probe can usually serve as an RFLP marker. 2. RFLP analysis requires a small amount of DNA. - a blood sample is usually enough to do many tests - can cult ...
Leukaemia Section t(1;21)(p32;q22) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... 1) partial karyotype and ideogram of t(1;21)(p32;q22) including a second copy of the der(1)t(1;21) present in the clone; and 2) metaphase FISH showing red AML1 signal on the two copies of the der(t)t(1;21), the der(21)t(1;21) and the normal 21 homolog. Green TEL signal is present of both 12 homologs ...
... 1) partial karyotype and ideogram of t(1;21)(p32;q22) including a second copy of the der(1)t(1;21) present in the clone; and 2) metaphase FISH showing red AML1 signal on the two copies of the der(t)t(1;21), the der(21)t(1;21) and the normal 21 homolog. Green TEL signal is present of both 12 homologs ...
Point Mutation Detection
... of genes are sequenced to detect a disease-specific mutation. Because the entire sequence of the human genome is known, PCR primers can be designed to amplify specific DNA for sequencing. Since DNA sequencing is greatly facilitated by abundant target DNA, only one application of PCR amplification of ...
... of genes are sequenced to detect a disease-specific mutation. Because the entire sequence of the human genome is known, PCR primers can be designed to amplify specific DNA for sequencing. Since DNA sequencing is greatly facilitated by abundant target DNA, only one application of PCR amplification of ...
RNA Detection and quantitation
... Primer design • Complementary to sequence of interest! Blast searching to improve specificity. • Length between 18-24 bps is optimal • Share similar melting temperatures (Tm) • Primer pair most not share complementarities at 3’ ends, primer dimer • No palindromic sequences, hairpin loops. • G/C con ...
... Primer design • Complementary to sequence of interest! Blast searching to improve specificity. • Length between 18-24 bps is optimal • Share similar melting temperatures (Tm) • Primer pair most not share complementarities at 3’ ends, primer dimer • No palindromic sequences, hairpin loops. • G/C con ...
Quantitative traits 1
... Nice theory. Is it true? (Classical test: breeding experiments) Edward East (1916) crossed pure breeding (inbred) lines of tobacco (Nicotiana longiflora) that differed in corolla height. The F1s were intermediate, but not significantly more variable than the parental lines. The F2s were also interm ...
... Nice theory. Is it true? (Classical test: breeding experiments) Edward East (1916) crossed pure breeding (inbred) lines of tobacco (Nicotiana longiflora) that differed in corolla height. The F1s were intermediate, but not significantly more variable than the parental lines. The F2s were also interm ...
Genome-wide association analysis with correlated traits in Duroc pigs
... as backfat thickness in pigs have been proven to be successful in certain breeding programs (Rothschild and Ruvinsky (2010)), however, knowledge of correlations for these traits on a molecular level remains unknown. Genomic improvement of feed efficiency in swine relies on identification of genomic ...
... as backfat thickness in pigs have been proven to be successful in certain breeding programs (Rothschild and Ruvinsky (2010)), however, knowledge of correlations for these traits on a molecular level remains unknown. Genomic improvement of feed efficiency in swine relies on identification of genomic ...
... Traditionally, Southern blots have been used to determine gene copy number (Southern, 1975). This typically involves extracting a significant quantity of genomic DNA, undergoing restriction digestions prior to blotting and probing. This is time consuming and often involves the use of 32P. Since its ...
Molecular Inversion Probe
![](https://en.wikipedia.org/wiki/Special:FilePath/MIP_probe_details_timothy_final.png?width=300)
Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping as well as for studying gene copy alterationsand characteristics of specific genomic loci to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.