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Supp Mat
... 10. Bandwidth of nanotube device The bandwidth of the nanotube device itself is also limited by extrinsic parasitics. The equivalent circuit is shown in Fig. S12. Here, RCNT is the resistance of the nanotube, Rp is the resistance in the leads (due to the resistivity of titanium), C is the capacitanc ...
... 10. Bandwidth of nanotube device The bandwidth of the nanotube device itself is also limited by extrinsic parasitics. The equivalent circuit is shown in Fig. S12. Here, RCNT is the resistance of the nanotube, Rp is the resistance in the leads (due to the resistivity of titanium), C is the capacitanc ...
Gene conversion analysis of the mouse Pilr locus
... Gene conversion analysis of the mouse Pilr locus. Gene conversion has played a role in shaping the mouse Pilr locus and is detected by a variety of phylogenetic and statistical methods. Phylogenetic analysis of a conserved 3 kb region (the promoter through to intron 3) suggests that Pilrb1 and Pilrb ...
... Gene conversion analysis of the mouse Pilr locus. Gene conversion has played a role in shaping the mouse Pilr locus and is detected by a variety of phylogenetic and statistical methods. Phylogenetic analysis of a conserved 3 kb region (the promoter through to intron 3) suggests that Pilrb1 and Pilrb ...
Figure S1 The yellow color of the Mimulus lewisii nectar
... RNAi lines but absent from the wild-type plant. The endogenous MlUBC gene was used as a control for genomic DNA quality. (B) Qualitative RT-PCR to test which MIXTA-like genes are expressed in the wild-type corolla during flower development––genes that are not expressed in the corolla should not affe ...
... RNAi lines but absent from the wild-type plant. The endogenous MlUBC gene was used as a control for genomic DNA quality. (B) Qualitative RT-PCR to test which MIXTA-like genes are expressed in the wild-type corolla during flower development––genes that are not expressed in the corolla should not affe ...
sv-lncs - acteon
... the statistical association between alleles at unlinked loci. SD arises, for example, by chance, due to population structure or small population size [e.g. 6]. In case-control studies, individuals are assumed unrelated. However, many false positive results arise when cases are more closely related t ...
... the statistical association between alleles at unlinked loci. SD arises, for example, by chance, due to population structure or small population size [e.g. 6]. In case-control studies, individuals are assumed unrelated. However, many false positive results arise when cases are more closely related t ...
MAX-BAX - Charles River Laboratories
... generations needed to produce a congenic strain, thereby producing a research model faster, while simultaneously reducing your facility, equipment and personnel costs. ...
... generations needed to produce a congenic strain, thereby producing a research model faster, while simultaneously reducing your facility, equipment and personnel costs. ...
Broad-range PCR tests
... • Value of BC in the diagnosis of sepsis is impaired by the delay in the time to results and the fact that positive BC can be found in only ± 30% of patients • Two categories of ‘rapid test’ that emerged for the detection of bacteria and fungi in blood: – Detection and identification of pathogens fr ...
... • Value of BC in the diagnosis of sepsis is impaired by the delay in the time to results and the fact that positive BC can be found in only ± 30% of patients • Two categories of ‘rapid test’ that emerged for the detection of bacteria and fungi in blood: – Detection and identification of pathogens fr ...
DNA polymerase - yusronsugiarto
... species). Increase stringency by increasing temp and decreasing salt concentration. • Rate of hybridization depends on DNA concentration and time (Cot), as well as GC content and DNA strand length. • Autoradiography. Put the labeled DNA next to X-ray film; the radiation fogs the ...
... species). Increase stringency by increasing temp and decreasing salt concentration. • Rate of hybridization depends on DNA concentration and time (Cot), as well as GC content and DNA strand length. • Autoradiography. Put the labeled DNA next to X-ray film; the radiation fogs the ...
Genome-wide scan with SNPs
... microsatellite markers spaced at intervals of approximately 10 cM across the genome. However, there is a growing realization that a map of closely spaced single-nucleotide polymorphisms (SNPs) may offer equal or superior power to detect linkage, compared with low-density microsatellite maps. Genome- ...
... microsatellite markers spaced at intervals of approximately 10 cM across the genome. However, there is a growing realization that a map of closely spaced single-nucleotide polymorphisms (SNPs) may offer equal or superior power to detect linkage, compared with low-density microsatellite maps. Genome- ...
GLYPHOSATE RESISTANCE Background / Problem
... relevant to real problems of evolutionary genetics as the study of the psychology of individuals isolated from their social context is to an understanding of man’s sociopolitical evolution” Richard Lewontin (quoted in Hedrick 2005) ...
... relevant to real problems of evolutionary genetics as the study of the psychology of individuals isolated from their social context is to an understanding of man’s sociopolitical evolution” Richard Lewontin (quoted in Hedrick 2005) ...
PDF
... to integrate D N A into the L. plantarum genome. The major advantage of this system, compared to the use of suicide vectors [3] is that the latter requires a high transformation frequency. In the approach described in this report, a tingle transformant, harbouring pSA3, when grown nonselectively wil ...
... to integrate D N A into the L. plantarum genome. The major advantage of this system, compared to the use of suicide vectors [3] is that the latter requires a high transformation frequency. In the approach described in this report, a tingle transformant, harbouring pSA3, when grown nonselectively wil ...
SNPs - Biology, Genetics and Bioinformatics Unit
... Haplotype: A set of closely linked genetic markers present on one chromosome which tend to be inherited together (not easily separable by recombination). Each person has two haplotypes in a given region, and each haplotype will be passed on as a complete unit ...
... Haplotype: A set of closely linked genetic markers present on one chromosome which tend to be inherited together (not easily separable by recombination). Each person has two haplotypes in a given region, and each haplotype will be passed on as a complete unit ...
Real time RT-PCR
... Real-time Principles •Based on the detection and quantitation of a fluorescent reporter * The first significant increase in the amount of PCR product (CT - threshold cycle) correlates to the initial amount of target template ...
... Real-time Principles •Based on the detection and quantitation of a fluorescent reporter * The first significant increase in the amount of PCR product (CT - threshold cycle) correlates to the initial amount of target template ...
ACMG Standards and Guidelines for constitutional cytogenomic
... detect. This should include both autosomal and sex chromosome abnormalities as duplications and deletions on the sex chromosomes may behave differently in each sex. Furthermore, blinding the evaluators to the expected abnormalities has the additional benefit of validating the settings, evaluation o ...
... detect. This should include both autosomal and sex chromosome abnormalities as duplications and deletions on the sex chromosomes may behave differently in each sex. Furthermore, blinding the evaluators to the expected abnormalities has the additional benefit of validating the settings, evaluation o ...
DHPS-WDR83 overlapping SNPs detection in QTL region for meat pH
... utilized approach for the detection of SNPs on transcribed sequences allowed to place approximately two to four markers for each cM, assuming that 1 cM corresponds, on average, to a segment of 1.3 Mb. This correspondence was obtained by comparing the total nucleotide length of the porcine genome of ...
... utilized approach for the detection of SNPs on transcribed sequences allowed to place approximately two to four markers for each cM, assuming that 1 cM corresponds, on average, to a segment of 1.3 Mb. This correspondence was obtained by comparing the total nucleotide length of the porcine genome of ...
Transposition - Pennsylvania State University
... • The RNA encoded by the retrotransposon is copied by reverse transcriptase into DNA • Primer for this synthesis can be generated by endonucleolytic cleavage at the target • Both reverse transcriptase and endonuclease are encoded by SOME (not all) retrotransposons • The 3’ end of the DNA strand at t ...
... • The RNA encoded by the retrotransposon is copied by reverse transcriptase into DNA • Primer for this synthesis can be generated by endonucleolytic cleavage at the target • Both reverse transcriptase and endonuclease are encoded by SOME (not all) retrotransposons • The 3’ end of the DNA strand at t ...
J.J. Bryan, V.L. Vance, M. Bauchet, C.L. Mouritsen
... – Samples (genotypes) are selected at random from each reference population and randomly placed in locations A-H in the P1 of a virtual pedigree. – Simulated mating occurs between the P1 pairs (A to B, C to D, etc.) wherein each SNP is considered independent from the results of all other at each int ...
... – Samples (genotypes) are selected at random from each reference population and randomly placed in locations A-H in the P1 of a virtual pedigree. – Simulated mating occurs between the P1 pairs (A to B, C to D, etc.) wherein each SNP is considered independent from the results of all other at each int ...
Interactions between SNP Alleles at Multiple Loci Contribute to Skin
... of Caucasoid subjects from the previous study [1] and the Mongoloid subjects in this study, we conducted the following analyses. 1) We determined the genotype and allele frequencies of the 20 SNPs observed in the Caucasoid (n=122) and Mongoloid (n=100) subjects to determine racial differences in dis ...
... of Caucasoid subjects from the previous study [1] and the Mongoloid subjects in this study, we conducted the following analyses. 1) We determined the genotype and allele frequencies of the 20 SNPs observed in the Caucasoid (n=122) and Mongoloid (n=100) subjects to determine racial differences in dis ...
$doc.title
... • 1-base Fluorescent, isotopic or Mass-spec* primer extension (Pastinen97) • 30-base extension Pyrosequencing (Ronaghi99)* • 700-base extension, capillary arrays dideoxy* (Tabor95, Nickerson97, Heiner98) ...
... • 1-base Fluorescent, isotopic or Mass-spec* primer extension (Pastinen97) • 30-base extension Pyrosequencing (Ronaghi99)* • 700-base extension, capillary arrays dideoxy* (Tabor95, Nickerson97, Heiner98) ...
docx
... for an interrogating base position includes five probes: one probe to hybridise to the WT, three probes to detect three possible single base-pair mutations, and one probe to detect single deletion. There are at least 24 probe sets for each nucleotide position, including both sense and anti-sense pro ...
... for an interrogating base position includes five probes: one probe to hybridise to the WT, three probes to detect three possible single base-pair mutations, and one probe to detect single deletion. There are at least 24 probe sets for each nucleotide position, including both sense and anti-sense pro ...
meiosis_6
... Flowers are always white unless one dominant allele is present at both loci This is because the first gene codes for an intermediate colourless pigment, if the dominant C allele is present The second gene codes for an enzyme that converts the intermediate compound to the purple pigment, if the domin ...
... Flowers are always white unless one dominant allele is present at both loci This is because the first gene codes for an intermediate colourless pigment, if the dominant C allele is present The second gene codes for an enzyme that converts the intermediate compound to the purple pigment, if the domin ...
Developing codominant PCR markers in pines
... primers might selectively amplify different rbcS genes, both were used for PCR reactions using both 2025 and 1635. The 2025-specific primer only produced a PCR fragment using 2025 template, and likewise for the 1635-specific primer. Even so, it appears that both 3' specific primers amplify more than ...
... primers might selectively amplify different rbcS genes, both were used for PCR reactions using both 2025 and 1635. The 2025-specific primer only produced a PCR fragment using 2025 template, and likewise for the 1635-specific primer. Even so, it appears that both 3' specific primers amplify more than ...
Document
... • Where is the SNP mapped? Exon, promoter, UTR, etc picture of gene with mapped to the gene structure. • How was it discovered? Method • What assurances do you have that it is real? Validated how? • What population – African, European, etc? • What is the allele frequency of each SNP? Common (>10%) ...
... • Where is the SNP mapped? Exon, promoter, UTR, etc picture of gene with mapped to the gene structure. • How was it discovered? Method • What assurances do you have that it is real? Validated how? • What population – African, European, etc? • What is the allele frequency of each SNP? Common (>10%) ...
Single nucleotide polymorphisms in candidate genes
... Goats are produced worldwide mainly because of their potential for milk and meat production, but they also can be used to provide leather, hair, and manure. Goats contribute to the development of rural areas and the well-being of their inhabitants (Horcada et al., 2012). Gastrointestinal nematode in ...
... Goats are produced worldwide mainly because of their potential for milk and meat production, but they also can be used to provide leather, hair, and manure. Goats contribute to the development of rural areas and the well-being of their inhabitants (Horcada et al., 2012). Gastrointestinal nematode in ...
Molecular Inversion Probe
![](https://en.wikipedia.org/wiki/Special:FilePath/MIP_probe_details_timothy_final.png?width=300)
Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping as well as for studying gene copy alterationsand characteristics of specific genomic loci to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.