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Print - Circulation Research
... Recently, we have demonstrated the expression of nonmuscle myosin in restenotic lesions in human coronary as well as peripheral arteries.25 The appearance of the nonmuscle isoform correlates with the newly acquired ability of smooth muscle cells to divide and disappears when cells return to their co ...
... Recently, we have demonstrated the expression of nonmuscle myosin in restenotic lesions in human coronary as well as peripheral arteries.25 The appearance of the nonmuscle isoform correlates with the newly acquired ability of smooth muscle cells to divide and disappears when cells return to their co ...
Investigation of the premelanosome protein
... Including intronic regions as described in Results and discussion. 2Information is related to the different primer pairs: amplified product size (bp)/annealing temperature (°C)/[MgCl2] (mM). For the primers used in the RACE experiments, only the specific PMEL primers are reported (PCR conditions are ...
... Including intronic regions as described in Results and discussion. 2Information is related to the different primer pairs: amplified product size (bp)/annealing temperature (°C)/[MgCl2] (mM). For the primers used in the RACE experiments, only the specific PMEL primers are reported (PCR conditions are ...
Structure of DIG
... Bio210 Fall, 2005 Detection by chemiluminescence: a complicated chain of events First: Incorporate “DIG” (Digoxigenin) into your probe DNA Structure of DIG: ...
... Bio210 Fall, 2005 Detection by chemiluminescence: a complicated chain of events First: Incorporate “DIG” (Digoxigenin) into your probe DNA Structure of DIG: ...
Gene Order Polymorphism in Yeast
... We would also like to examine a. How transposition of the five genes affects their gene expression b. What the frequency of this rearrangement is among a larger sample of natural yeast strains c. Whether there are any clues as to the transposition mechanism in the sequences in and around the transpo ...
... We would also like to examine a. How transposition of the five genes affects their gene expression b. What the frequency of this rearrangement is among a larger sample of natural yeast strains c. Whether there are any clues as to the transposition mechanism in the sequences in and around the transpo ...
Package `LDheatmap`
... been adopted, in which horizontal coordinates correspond to the rows of the matrix and vertical coordinates correspond to columns, and vertical coordinates are indexed in increasing order from bottom to top. For the argument color, an appropriate color palette for quantitative data is recommended, a ...
... been adopted, in which horizontal coordinates correspond to the rows of the matrix and vertical coordinates correspond to columns, and vertical coordinates are indexed in increasing order from bottom to top. For the argument color, an appropriate color palette for quantitative data is recommended, a ...
Integrated genomic DNA/RNA profiling of
... insertions/deletions (indels), copy number alterations (CNAs), and a wide range of gene fusions; no current clinically available single assay captures the different types of alterations. We developed a novel next-generation sequencing-based assay to identify all classes of genomic alterations using ...
... insertions/deletions (indels), copy number alterations (CNAs), and a wide range of gene fusions; no current clinically available single assay captures the different types of alterations. We developed a novel next-generation sequencing-based assay to identify all classes of genomic alterations using ...
Molecular Diagnostics 6
... Added to every sample before nucleic acid extraction Will ensure that extraction and amplification procedures are acceptable A second set of primers must also be added to the reaction for this control to be amplified. The procedure must be optimized such that the amplification of the control does no ...
... Added to every sample before nucleic acid extraction Will ensure that extraction and amplification procedures are acceptable A second set of primers must also be added to the reaction for this control to be amplified. The procedure must be optimized such that the amplification of the control does no ...
Isolation and Characterization of a Cytochrome P450 Gene from
... expression of blue or purple flower color. The F3’5’H genes have already been cloned from several dicots (Mori et al., 2004), whereas isolation of the counterparts from monocots has not yet been reported. Recently, molecular breeding for flower color modification has been performed in some dicot orn ...
... expression of blue or purple flower color. The F3’5’H genes have already been cloned from several dicots (Mori et al., 2004), whereas isolation of the counterparts from monocots has not yet been reported. Recently, molecular breeding for flower color modification has been performed in some dicot orn ...
The International Tomato Sequencing Project and Related
... Rod Wing and Wellcome Trust Sanger Institute ...
... Rod Wing and Wellcome Trust Sanger Institute ...
A physical map of the genome of Hmmophilus
... on the DNA contained in one-third of a complete plug. Restriction einzyme buffers were diffused into the agarose blocks as outlined below. Plugs or portions of plugs were washed in Eppendorf tubes with 500 1.11 vlolumesof buffer (unless stated otherwise). Fresh buffer was used for each wash. Two 30 ...
... on the DNA contained in one-third of a complete plug. Restriction einzyme buffers were diffused into the agarose blocks as outlined below. Plugs or portions of plugs were washed in Eppendorf tubes with 500 1.11 vlolumesof buffer (unless stated otherwise). Fresh buffer was used for each wash. Two 30 ...
"PCR-based Species Identification and Gene Tagging"
... maize leaf _Bots infected leaf pt3 infected leaf pt1 P sorghi pt3 P sorghi pt1 Marker ...
... maize leaf _Bots infected leaf pt3 infected leaf pt1 P sorghi pt3 P sorghi pt1 Marker ...
Section 3: Prokaryotic Sample and Array Processing
... As starting material for the cDNA synthesis procedure, total RNA can be isolated by using standard procedures for bacterial RNA isolation or various commercial RNA isolation kits. For Pseudomonas aeruginosa and E. coli, we have successfully used the QIAGEN® RNeasy Mini Purification Kit. Caution shou ...
... As starting material for the cDNA synthesis procedure, total RNA can be isolated by using standard procedures for bacterial RNA isolation or various commercial RNA isolation kits. For Pseudomonas aeruginosa and E. coli, we have successfully used the QIAGEN® RNeasy Mini Purification Kit. Caution shou ...
Diagnostic Testing for Prader-Willi and Angelman
... Overview Prader-Willi syndrome (PWS) is a complex disorder whose diagnosis may be difficult to establish on clinical grounds and whose genetic basis is heterogeneous. Slightly >70% of cases are due to a 15q11q13 deletion in the paternally contributed chromosome. These deletions are optimally detecte ...
... Overview Prader-Willi syndrome (PWS) is a complex disorder whose diagnosis may be difficult to establish on clinical grounds and whose genetic basis is heterogeneous. Slightly >70% of cases are due to a 15q11q13 deletion in the paternally contributed chromosome. These deletions are optimally detecte ...
Mutations in a gene encoding a novel protein tyrosine
... Fig. 4). A single cDNA (266552) representing transcript B shared identity with transcript A, except for the omission of a 1,770-bp segment due to splicing (Figs 3, 4). The common origin of transcripts A and B suggests they are alternative forms of the same gene, the products of which would be predic ...
... Fig. 4). A single cDNA (266552) representing transcript B shared identity with transcript A, except for the omission of a 1,770-bp segment due to splicing (Figs 3, 4). The common origin of transcripts A and B suggests they are alternative forms of the same gene, the products of which would be predic ...
Identification of R-Gene Homologous DNA Fragments Genetically
... different clones were determined. Sequence comparison of R-gene homologous DNA fragments. In total, four fragments from Col (C1 to C4) and four from Ler (L1 to L4) were sequenced. These fragments were grouped in three classes (C1 [0.5 kb]; L1, L2, C2, C3 [0.5 kb]; and L3, L4, C4 [0.8 kb]) based on t ...
... different clones were determined. Sequence comparison of R-gene homologous DNA fragments. In total, four fragments from Col (C1 to C4) and four from Ler (L1 to L4) were sequenced. These fragments were grouped in three classes (C1 [0.5 kb]; L1, L2, C2, C3 [0.5 kb]; and L3, L4, C4 [0.8 kb]) based on t ...
Novel data clustering for microarrays and image segmentation
... Oligonucleotide sequences (oligos) probes: 25 nucleotide chains for selected parts of a gene complementary to mRNA. For every gene there are 1120(depending on chip design) of different oligo probes called perfect matches (PM). In addition, there are mismatch oligos (MM) corresponding to each of the ...
... Oligonucleotide sequences (oligos) probes: 25 nucleotide chains for selected parts of a gene complementary to mRNA. For every gene there are 1120(depending on chip design) of different oligo probes called perfect matches (PM). In addition, there are mismatch oligos (MM) corresponding to each of the ...
Segmented Arrangement of Borrelia duttonii DNA
... inserts are identical. Thus, a total of three different oligonucleotide selected sequences have been cloned; the cross-hybridization data show that these contain no shared sequences substantially larger than the sequence selected by the oligonucleotide probe. Probing uncleaved B. duttonii DNA The VS ...
... inserts are identical. Thus, a total of three different oligonucleotide selected sequences have been cloned; the cross-hybridization data show that these contain no shared sequences substantially larger than the sequence selected by the oligonucleotide probe. Probing uncleaved B. duttonii DNA The VS ...
Genetic variants associated with breast size also influence breast
... SNPs are in linkage disequilibrium (LD) with SNPs associated with breast cancer (those near ESR1 and PTHLH), and a third (ZNF365) is near, but not in LD with, a SNP associated with breast cancer.! ...
... SNPs are in linkage disequilibrium (LD) with SNPs associated with breast cancer (those near ESR1 and PTHLH), and a third (ZNF365) is near, but not in LD with, a SNP associated with breast cancer.! ...
Application of PCR-technique in biological labs
... When these genes are expressed in prokaryotic cells for protein production or purification, the RNA produced from transcription need not undergo splicing as it contains only exons. ...
... When these genes are expressed in prokaryotic cells for protein production or purification, the RNA produced from transcription need not undergo splicing as it contains only exons. ...
Drosophila - The Solomon H Snyder Department of Neuroscience
... then performed to amplify the genomic sequence between the transposon insertion and the annealed splinkerette. This is followed by a sequencing reaction with another nested primer. The spPCR reaction remains highly efficient and specific due to the splinkerette design. Since the splinkerette oligonu ...
... then performed to amplify the genomic sequence between the transposon insertion and the annealed splinkerette. This is followed by a sequencing reaction with another nested primer. The spPCR reaction remains highly efficient and specific due to the splinkerette design. Since the splinkerette oligonu ...
Genetic Services-Intellectual Disability Project
... A total of 9 abnormalities were detected by MLPA. Seven abnormalities were previously reported deletions and derivative chromosomes. Two abnormalities were novel, and not detected by FISH with the ToTel Vysion FISH probe panel. Subsequent FISH analysis with additional DNA sequences from the regions ...
... A total of 9 abnormalities were detected by MLPA. Seven abnormalities were previously reported deletions and derivative chromosomes. Two abnormalities were novel, and not detected by FISH with the ToTel Vysion FISH probe panel. Subsequent FISH analysis with additional DNA sequences from the regions ...
Molecular Inversion Probe
![](https://en.wikipedia.org/wiki/Special:FilePath/MIP_probe_details_timothy_final.png?width=300)
Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping as well as for studying gene copy alterationsand characteristics of specific genomic loci to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.