rodrigo_brindeirox
... – Amplicons HIV: 17 (3 GAG, 2 PR, 6 RT, 3 IN, 2 ENVgp41, 1 ENVgp120) ou, 11 (2 GAG, 1 PR, 4 RT, 2 IN, 1 ENVgp41, 1 ENVgp120) – Amplicons HCV: 10 (4 PR, 6 Rep) ou, 6 (2 PR, 4 Rep) ...
... – Amplicons HIV: 17 (3 GAG, 2 PR, 6 RT, 3 IN, 2 ENVgp41, 1 ENVgp120) ou, 11 (2 GAG, 1 PR, 4 RT, 2 IN, 1 ENVgp41, 1 ENVgp120) – Amplicons HCV: 10 (4 PR, 6 Rep) ou, 6 (2 PR, 4 Rep) ...
CDKN2 (p16/MTS1) Gene Deletion or CDK4
... tively short distance in a number of the tumors. Breakpoints occurred between the MTS2 locus and the CDKN2 locus in GB25, GB29, and GB14, and between CDKN2 and 1063. 7 in GB23, AA18, and AA49. The common region of loss, when data from both the homozygous deletions and losses of one allele are combin ...
... tively short distance in a number of the tumors. Breakpoints occurred between the MTS2 locus and the CDKN2 locus in GB25, GB29, and GB14, and between CDKN2 and 1063. 7 in GB23, AA18, and AA49. The common region of loss, when data from both the homozygous deletions and losses of one allele are combin ...
Experimental General. All the DNA manipulations and bacterial
... Together with the above mutagenic primers, in the first PCRs, BC-LIP-9F (5’CCGCCACGTACAACCAGAACTATC-3’) and PET-2R (5’-GTTATTGCTCAGCGGTGG3’) were also used, and in the second PCR, BC-LIP-9F and PET-2R were used. The conditions for the 100 µL PCR mixture were as follows: 0.5 µM each primer, 0.2 mM ea ...
... Together with the above mutagenic primers, in the first PCRs, BC-LIP-9F (5’CCGCCACGTACAACCAGAACTATC-3’) and PET-2R (5’-GTTATTGCTCAGCGGTGG3’) were also used, and in the second PCR, BC-LIP-9F and PET-2R were used. The conditions for the 100 µL PCR mixture were as follows: 0.5 µM each primer, 0.2 mM ea ...
WASP - Genome Institute, BIOTEC
... One of the results from human genome project is the use of human variation data called single nucleotide polymorphism (SNP). Such polymorphisms including rare ones such as mutations reflect how we have different genetic responses to the environment as well as predispositions to certain diseases and ...
... One of the results from human genome project is the use of human variation data called single nucleotide polymorphism (SNP). Such polymorphisms including rare ones such as mutations reflect how we have different genetic responses to the environment as well as predispositions to certain diseases and ...
PraderWilli syndrome resulting from an unbalanced translocation
... by the map position of the deleted BAC and the flanking BAC. In addition to the deletion on chromosome 5p, a deletion of the long arm of one copy of chromosome 15 was present, as indicated by five BACs (Fig. 5b) with the average log2 ratio ¼ 0.82 0.09. This deletion was concordant with the standa ...
... by the map position of the deleted BAC and the flanking BAC. In addition to the deletion on chromosome 5p, a deletion of the long arm of one copy of chromosome 15 was present, as indicated by five BACs (Fig. 5b) with the average log2 ratio ¼ 0.82 0.09. This deletion was concordant with the standa ...
Characterization of two genes encoding the mitochondrial
... primers 3 and 4, designed from two different exons, a PCR amplification of a C. reinhardtii cDNA library yielded a fragment of the expected size (173 bp) and sequence. This fragment was used as a probe to screen a cDNA library and allowed the isolation of six clones (with inserts up to 2.2 kb), all ...
... primers 3 and 4, designed from two different exons, a PCR amplification of a C. reinhardtii cDNA library yielded a fragment of the expected size (173 bp) and sequence. This fragment was used as a probe to screen a cDNA library and allowed the isolation of six clones (with inserts up to 2.2 kb), all ...
Shastry, B.S. 2002. SNP alleles in human disease and evolution
... SNPs in gene discovery Once the map of these SNPs is confirmed, they can be used for evolutionary biology studies, gene discovery and mapping, prediction of drug and environmental response, diagnostic tests, heterogeneity testing, and association studies (Gray et al. 2000; Schork et al. 2000). For t ...
... SNPs in gene discovery Once the map of these SNPs is confirmed, they can be used for evolutionary biology studies, gene discovery and mapping, prediction of drug and environmental response, diagnostic tests, heterogeneity testing, and association studies (Gray et al. 2000; Schork et al. 2000). For t ...
hered master 4..hered 285 .. Page78
... (Table 1). This was caused primarily by the high frequency of the Lolium a allele (27 per cent) which was recovered at greater frequency than any other PGI/2 allele (16–19 per cent). The three Festuca PGI/2 alleles were recovered at similar frequency. Almost all plants (98 per cent) carried at least ...
... (Table 1). This was caused primarily by the high frequency of the Lolium a allele (27 per cent) which was recovered at greater frequency than any other PGI/2 allele (16–19 per cent). The three Festuca PGI/2 alleles were recovered at similar frequency. Almost all plants (98 per cent) carried at least ...
Identification of novel endogenous antisense transcripts by DNA
... finding indicates that antisense transcriptome analyses based solely on cDNA information may be inefficient. In addition, most publicly available cDNA sequences are derived from normal cellular conditions, such as normal adult tissues, and thus are not useful for the identification of NATs specific ...
... finding indicates that antisense transcriptome analyses based solely on cDNA information may be inefficient. In addition, most publicly available cDNA sequences are derived from normal cellular conditions, such as normal adult tissues, and thus are not useful for the identification of NATs specific ...
DLBCL PAC Pitch - World CDx Boston 2016
... Data show that ABC have worse outcome relative to GCB-type tumors when treated with R-CHOP ...
... Data show that ABC have worse outcome relative to GCB-type tumors when treated with R-CHOP ...
Resources for the map-based cloning of tga1
... We sequenced the tga1 promoter and coding regions for a set of 16 diverse landraces of maize and 12 teosinte individuals (Z. mays ssp. parviglumis) and the outgroup Zea diploperennis (Genbank AY883436-AY883558) using the PCR primers and conditions listed above. PCR products from Z. diploperennis wer ...
... We sequenced the tga1 promoter and coding regions for a set of 16 diverse landraces of maize and 12 teosinte individuals (Z. mays ssp. parviglumis) and the outgroup Zea diploperennis (Genbank AY883436-AY883558) using the PCR primers and conditions listed above. PCR products from Z. diploperennis wer ...
(PPI) node degrees with SNP counts
... which contains information about the genes associated with each SNP. The Build 128 version of SNPContigLocusID contains about 13,129,868 rows (though about half of them specify “NW_” mRNA segments and were ignored). Here is a query that retrieves the records for 2 SNPs (among many others) that appea ...
... which contains information about the genes associated with each SNP. The Build 128 version of SNPContigLocusID contains about 13,129,868 rows (though about half of them specify “NW_” mRNA segments and were ignored). Here is a query that retrieves the records for 2 SNPs (among many others) that appea ...
THE IDENTIFICATION AND CHARACTERISATION OF THE
... The sequences of clones A-18 and A-66 were identical and determined by the NRPSpredictor 2 to be specific for phenylalanine. Protein BLAST analysis of the amino acid sequence of both clones showed a high similarity to an amino acid adenylation protein in Granuliella mallensis MP5ACIX8 (YP_00507340.1 ...
... The sequences of clones A-18 and A-66 were identical and determined by the NRPSpredictor 2 to be specific for phenylalanine. Protein BLAST analysis of the amino acid sequence of both clones showed a high similarity to an amino acid adenylation protein in Granuliella mallensis MP5ACIX8 (YP_00507340.1 ...
Probing chromatin-modifying enzymes with chemical tools Wolfgang
... physiological pH and has been useful to profile substrates of a variety of PKMTs and PRMTs. Interestingly, for certain PMTs the alkyne-SeAM analogues are better cosubstrates compared to their SAM equivalents suggesting that sulfonium-to selenonium substitution can enhance reaction rates of otherwise ...
... physiological pH and has been useful to profile substrates of a variety of PKMTs and PRMTs. Interestingly, for certain PMTs the alkyne-SeAM analogues are better cosubstrates compared to their SAM equivalents suggesting that sulfonium-to selenonium substitution can enhance reaction rates of otherwise ...
A rapid and specific PCR–ELISA for detecting Salmonella typhi
... to bacteriological techniques. Our findings indicate that this approach could become an international standard and could be employed with confidence in microbiologic laboratories. The conventional PCR assay is already an established laboratory technique, but adaptation of the test in a PCR-ELISA for ...
... to bacteriological techniques. Our findings indicate that this approach could become an international standard and could be employed with confidence in microbiologic laboratories. The conventional PCR assay is already an established laboratory technique, but adaptation of the test in a PCR-ELISA for ...
Abstract Citrus is the main fruit crop in the world and Spain is the 6th
... parental heterozygosity restitution (HR) in the diploid gamete at each locus, which is mainly affected by the triploid recovery strategy. In 2x × 2x crosses, HR depends on the underlying mechanism leading to the unreduced gamete formation, which are genetically equivalent to First Division Restituti ...
... parental heterozygosity restitution (HR) in the diploid gamete at each locus, which is mainly affected by the triploid recovery strategy. In 2x × 2x crosses, HR depends on the underlying mechanism leading to the unreduced gamete formation, which are genetically equivalent to First Division Restituti ...
PCR
... Magnesium ions are critical -Magnesium ion concentration often needs optimization exists as dNTP-Mg2+ complexes, interacts with DNA backbone influences activity of Taq polymerase; MgCl2 is used in buffer to adjust concentrations Between 0.5 and 5mM is generally used (1.5 mM common) Low concentratio ...
... Magnesium ions are critical -Magnesium ion concentration often needs optimization exists as dNTP-Mg2+ complexes, interacts with DNA backbone influences activity of Taq polymerase; MgCl2 is used in buffer to adjust concentrations Between 0.5 and 5mM is generally used (1.5 mM common) Low concentratio ...
Inheritance of Nuclear DNA Markers in Gynogenetic Haploid Pink
... as a result of their polyploid ancestry, have more duplicated loci. PCR primers designed without detailed knowledge of differences between paralogous loci may or may not amplify sequences from both loci. Moreover, even if only one locus is amplified, it will be difficult to ensure that homologous lo ...
... as a result of their polyploid ancestry, have more duplicated loci. PCR primers designed without detailed knowledge of differences between paralogous loci may or may not amplify sequences from both loci. Moreover, even if only one locus is amplified, it will be difficult to ensure that homologous lo ...
Imprinted SNRPN within chromosome territories
... cells were fractionated by centrifugal elutriation. Cultured cells (3×108 cells) were loaded onto a Beckman elutriator rotor at 2,000 rpm at 4°C with flow of 0.3% gelatin in PBS (phosphate-buffered saline). The flow rate was increased from 11 ml/minute to 26 ml/minute at every 2.5 ml/minute incremen ...
... cells were fractionated by centrifugal elutriation. Cultured cells (3×108 cells) were loaded onto a Beckman elutriator rotor at 2,000 rpm at 4°C with flow of 0.3% gelatin in PBS (phosphate-buffered saline). The flow rate was increased from 11 ml/minute to 26 ml/minute at every 2.5 ml/minute incremen ...
0 1 0 1 1 1 0 0 1 0
... – The entropy of a data set is given by - p log2(p) - (1 - p) log2(1 - p) where p is the fraction of examples that belong to class predisposed. – The information gain of the split is given by the entropy of the original data set minus the weighted sum of entropies of the two data sets resulting from ...
... – The entropy of a data set is given by - p log2(p) - (1 - p) log2(1 - p) where p is the fraction of examples that belong to class predisposed. – The information gain of the split is given by the entropy of the original data set minus the weighted sum of entropies of the two data sets resulting from ...
Literature - Oxford Gene Technology
... Lung cancer is the most commonly diagnosed cancer as well as the leading cause of cancer death in males. Worldwide, lung cancer accounted for 1.82 million new cases and 1.58 million deaths in 20121. Recent advances have been made in the diagnosis of lung cancer and the use of genomic technologies fo ...
... Lung cancer is the most commonly diagnosed cancer as well as the leading cause of cancer death in males. Worldwide, lung cancer accounted for 1.82 million new cases and 1.58 million deaths in 20121. Recent advances have been made in the diagnosis of lung cancer and the use of genomic technologies fo ...
Poster Patrocles_V3
... appreciable effect on gene function will evolve neutrally, subject only to the vagaries of random genetic drift while pSNPs affecting gene function may undergo positive, negative or balancing selection via their effect on phenotype. Selection may leave distinct signatures on the level of inter-speci ...
... appreciable effect on gene function will evolve neutrally, subject only to the vagaries of random genetic drift while pSNPs affecting gene function may undergo positive, negative or balancing selection via their effect on phenotype. Selection may leave distinct signatures on the level of inter-speci ...
Major influence of repetitive elements on disease
... presents examples of high copy repeats that have been detected at the breakpoints of disease-associated CNVs. Borun and colleagues [23] reported the presence of CNV breakpoints within Alu elements in the STK11 gene which lead to the Peutz-Jeghers syndrome (OMIM #175200), where CNVs account for 30 % ...
... presents examples of high copy repeats that have been detected at the breakpoints of disease-associated CNVs. Borun and colleagues [23] reported the presence of CNV breakpoints within Alu elements in the STK11 gene which lead to the Peutz-Jeghers syndrome (OMIM #175200), where CNVs account for 30 % ...
Molecular Inversion Probe
Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping as well as for studying gene copy alterationsand characteristics of specific genomic loci to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.