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... Abstract. Methods designed to detect microorganisms involved in the biogeochemistry of nitrogen in the marine environment are rapidly being developed and deployed in ecological investigations. Probes based on phylogenetic sequences (usually rRNA) and those based on the sequences of functional genes ...
... Abstract. Methods designed to detect microorganisms involved in the biogeochemistry of nitrogen in the marine environment are rapidly being developed and deployed in ecological investigations. Probes based on phylogenetic sequences (usually rRNA) and those based on the sequences of functional genes ...
SNP rs2157719 in the CDKN2B-AS1 gene gene
... through epigenetic mechanisms and implicated in several other diseases [4-6]. In addition, the expression of CDKN2B/AS1 is dramatically induced by transforming growth factor (TGF)-β which is known to play a critical role in POAG pathogenesis [7,8]. Several genome-wide association studies (GWASs) and ...
... through epigenetic mechanisms and implicated in several other diseases [4-6]. In addition, the expression of CDKN2B/AS1 is dramatically induced by transforming growth factor (TGF)-β which is known to play a critical role in POAG pathogenesis [7,8]. Several genome-wide association studies (GWASs) and ...
Example - Hivebench
... For example, there is a ¼ chance (4-1) of finding an A, G, C or T in any given DNA sequence; there is a 1/16 chance (4-2) of finding any dinucleotide sequence (e.g. AG); a 1/256 chance of finding a given 4-base sequence. Thus, a sixteen base sequence will statistically be present only once in every ...
... For example, there is a ¼ chance (4-1) of finding an A, G, C or T in any given DNA sequence; there is a 1/16 chance (4-2) of finding any dinucleotide sequence (e.g. AG); a 1/256 chance of finding a given 4-base sequence. Thus, a sixteen base sequence will statistically be present only once in every ...
A missense mutation in growth differentiation factor 9 (GDF9) is
... Background: A genome wide association study for litter size in Norwegian White Sheep (NWS) was conducted using the recently developed ovine 50K SNP chip from Illumina. After genotyping 378 progeny tested artificial insemination (AI) rams, a GWAS analysis was performed on estimated breeding values (E ...
... Background: A genome wide association study for litter size in Norwegian White Sheep (NWS) was conducted using the recently developed ovine 50K SNP chip from Illumina. After genotyping 378 progeny tested artificial insemination (AI) rams, a GWAS analysis was performed on estimated breeding values (E ...
Identification of Vietnamese Coptotermes pest species based on the
... Coptotermes, found in urban areas, is regarded as the most abundant building termite pest genus, widely distributed in Vietnam. The objectives of this study were to classify the Coptotermes found in certain provinces in Vietnam and assess the feasibility proposed PCR method by Szalanski et al., 2004 ...
... Coptotermes, found in urban areas, is regarded as the most abundant building termite pest genus, widely distributed in Vietnam. The objectives of this study were to classify the Coptotermes found in certain provinces in Vietnam and assess the feasibility proposed PCR method by Szalanski et al., 2004 ...
Fatty Acids - Mayo Clinic
... HIV-1 RNA quantitation is performed by PCR using the Roche Amplicor System. Plasma is chemically extracted and the viral RNA is precipitated with isopropanol. A known amount of a standard synthetic RNA molecule is added to each specimen to permit quantitation of HIV RNA by a comparison of resulting ...
... HIV-1 RNA quantitation is performed by PCR using the Roche Amplicor System. Plasma is chemically extracted and the viral RNA is precipitated with isopropanol. A known amount of a standard synthetic RNA molecule is added to each specimen to permit quantitation of HIV RNA by a comparison of resulting ...
Plumage Genes and Little Else Distinguish the Genomes of
... the genome. We also found that the divergent regions had, on average, higher levels of absolute divergence (e.g., dxy; Figure S3). The disproportionate extent and number of divergence peaks associated with the Z chromosome are likely due to its reduced Ne and recombination rate but also may be due t ...
... the genome. We also found that the divergent regions had, on average, higher levels of absolute divergence (e.g., dxy; Figure S3). The disproportionate extent and number of divergence peaks associated with the Z chromosome are likely due to its reduced Ne and recombination rate but also may be due t ...
Supplementary Information (doc 1628K)
... each sample, the 3’ IVT Express Kit (Affymetrix, Santa Clara, CA) synthesizes biotin – label cRNA target from 100ng of total RNA. A hybridization cocktail whic target is created for each sample. The samples are hybridized to a standard Probe Array Cartidge (GeneChip Primeview Human Gene Expression A ...
... each sample, the 3’ IVT Express Kit (Affymetrix, Santa Clara, CA) synthesizes biotin – label cRNA target from 100ng of total RNA. A hybridization cocktail whic target is created for each sample. The samples are hybridized to a standard Probe Array Cartidge (GeneChip Primeview Human Gene Expression A ...
Supplementary Material for Autozygome Sequencing Expands the
... script in preparation for loading into our database. Essentially we extracted a slightly summarized view of the dataset removing non-autosomal alleles and redundant reports (by grouping on allele position and mutation type). We also anchored the variant calls to the reference genome allele and calcu ...
... script in preparation for loading into our database. Essentially we extracted a slightly summarized view of the dataset removing non-autosomal alleles and redundant reports (by grouping on allele position and mutation type). We also anchored the variant calls to the reference genome allele and calcu ...
L- Amino Acid Assay Kit (Colorimetric)
... When consumed and absorbed by the human body, the standard amino acids are used to make proteins and other molecules or are oxidized to urea and carbon dioxide to be used as a form of energy. The oxidation pathway begins with transamidase removal of the amino group, and this group is then processed ...
... When consumed and absorbed by the human body, the standard amino acids are used to make proteins and other molecules or are oxidized to urea and carbon dioxide to be used as a form of energy. The oxidation pathway begins with transamidase removal of the amino group, and this group is then processed ...
genes is conserved among species related to
... the gastro-intestinal tract of the host. In this study we have determined whether closely related strains of L. acidopbih.~contain an S protein and corresponding gene(s) that are similar to those of L. aciduphilzls. Our results show that L.crispatas, L. am_ylovur&lsand L.gallimradrn have two sr'p re ...
... the gastro-intestinal tract of the host. In this study we have determined whether closely related strains of L. acidopbih.~contain an S protein and corresponding gene(s) that are similar to those of L. aciduphilzls. Our results show that L.crispatas, L. am_ylovur&lsand L.gallimradrn have two sr'p re ...
Development of an internally controlled real-time PCR
... internally controlled real-time PCR assay to detect C. psittaci DNA in the LightCycler 2.0 system using two TaqMan probes. The IC monitored the process of nucleic acid purification and amplification for each individual sample. After liquefaction of sputum samples and subsequent Boom extraction, it w ...
... internally controlled real-time PCR assay to detect C. psittaci DNA in the LightCycler 2.0 system using two TaqMan probes. The IC monitored the process of nucleic acid purification and amplification for each individual sample. After liquefaction of sputum samples and subsequent Boom extraction, it w ...
A genome-wide association scan in pig identifies novel regions
... EBV (born in 1998 to 2010) using the Illumina Porcine SNP60 BeadChip (Illumina). The SNP data were editHG XVLQJ WKH VDPH FULWHULD DV LQ 2VWHUVHQ HW DO Each animal had a call rate greater than 0.95, a minor allele frequency greater than 0.05, a call-frequency score greater than 0. ...
... EBV (born in 1998 to 2010) using the Illumina Porcine SNP60 BeadChip (Illumina). The SNP data were editHG XVLQJ WKH VDPH FULWHULD DV LQ 2VWHUVHQ HW DO Each animal had a call rate greater than 0.95, a minor allele frequency greater than 0.05, a call-frequency score greater than 0. ...
Linkage Mapping and Molecular Diversity at the Flower Sex Locus
... The primer sequences for SNP discovery and genotyping are reported in Table 1. Details of the procedure for DNA extraction, marker amplification, segregation analysis and map construction are given in Moreira et al. [12]. Polymorphism at microsatellite loci in the sex genomic region was evaluated in ...
... The primer sequences for SNP discovery and genotyping are reported in Table 1. Details of the procedure for DNA extraction, marker amplification, segregation analysis and map construction are given in Moreira et al. [12]. Polymorphism at microsatellite loci in the sex genomic region was evaluated in ...
Amplification and partial sequencing of Ixodes Scapularis Shaker
... passing pathogens to humans that cause lyme disease, rocky mountain spotted fever and tularemia (9). The research project undertaken provides an easy and efficient means to begin the tick DNA sequencing on a small scale, which can be done in almost any molecular biology laboratory. The sequence homo ...
... passing pathogens to humans that cause lyme disease, rocky mountain spotted fever and tularemia (9). The research project undertaken provides an easy and efficient means to begin the tick DNA sequencing on a small scale, which can be done in almost any molecular biology laboratory. The sequence homo ...
Fruit shape - UC Davis Plant Sciences
... • Nucleotide polymorphisms should be high between two parents • Two mapping populations • EPM= L. esculentum Sun1642 x L. pimpinellifolium LA1589 • These two lines are inbred • EPN= L. esculentum Sun1642 x L. esculentum IL7-4 • IL7-4 has segment of chromosome 7 of L. pennellii LA716 • EPN is nearly ...
... • Nucleotide polymorphisms should be high between two parents • Two mapping populations • EPM= L. esculentum Sun1642 x L. pimpinellifolium LA1589 • These two lines are inbred • EPN= L. esculentum Sun1642 x L. esculentum IL7-4 • IL7-4 has segment of chromosome 7 of L. pennellii LA716 • EPN is nearly ...
Text S1.
... Based on the mismatch distribution for interspecific pairs of SRK sequences between A. lyrata and A. halleri (Fig. 2), we defined "trans-specifically shared S-alleles", i.e. alleles assumed to have evolved from a single S-allele in the direct ancestor of A. lyrata and A. halleri, as those whose sequ ...
... Based on the mismatch distribution for interspecific pairs of SRK sequences between A. lyrata and A. halleri (Fig. 2), we defined "trans-specifically shared S-alleles", i.e. alleles assumed to have evolved from a single S-allele in the direct ancestor of A. lyrata and A. halleri, as those whose sequ ...
letters - Lewis-Sigler Institute | for Integrative Genomics
... rank-sum statistic was formed to quantify expression differences between the segregants grouped by inheritance at the locus. We identified the ‘primary’ QTL for each transcript as the locus with the most significant Wilcoxon rank-sum statistic. We then partitioned segregants on the basis of inherita ...
... rank-sum statistic was formed to quantify expression differences between the segregants grouped by inheritance at the locus. We identified the ‘primary’ QTL for each transcript as the locus with the most significant Wilcoxon rank-sum statistic. We then partitioned segregants on the basis of inherita ...
GBS Pipeline Documentation. - WSU Plant Pathology
... Genotypes: This folder has a total of 10 different files. Some of these contain genotype calls for the parents against the population. These calls include unique information about the SNPs, such as SNP position and chromosome assignments, when compared to the Chinese Spring draft assembly and the Wa ...
... Genotypes: This folder has a total of 10 different files. Some of these contain genotype calls for the parents against the population. These calls include unique information about the SNPs, such as SNP position and chromosome assignments, when compared to the Chinese Spring draft assembly and the Wa ...
IBDfinder and SNPsetter: tools for pedigree
... notable criterion, such as homozygosity for a rare allele. The region identified as IBD will then differ according to the direction in which the chromosome is scanned. Scanning in both directions will identify two different (overlapping or non-overlapping) intervals, which if combined define a regi ...
... notable criterion, such as homozygosity for a rare allele. The region identified as IBD will then differ according to the direction in which the chromosome is scanned. Scanning in both directions will identify two different (overlapping or non-overlapping) intervals, which if combined define a regi ...
Methods S1.
... The aim of MDR is to assign each of the nine possible genotype combinations for two SNPs to two classes (high and low risk) and to select the pair of SNPs that best separates the cases and controls, so that cases tend to be classified as high risk and controls as low risk. MDR uses 10-fold cross val ...
... The aim of MDR is to assign each of the nine possible genotype combinations for two SNPs to two classes (high and low risk) and to select the pair of SNPs that best separates the cases and controls, so that cases tend to be classified as high risk and controls as low risk. MDR uses 10-fold cross val ...
Evidence for allelism of the recessive insertional
... the gene product is essential in other processes or there might be other gene(s) within the deletion that are recessive mutated, not involved in limb formation, but essential for normal development. At present we are attempting to detect transcript alterations in the Xt and/or add mice using DNA pro ...
... the gene product is essential in other processes or there might be other gene(s) within the deletion that are recessive mutated, not involved in limb formation, but essential for normal development. At present we are attempting to detect transcript alterations in the Xt and/or add mice using DNA pro ...
Unified display of Arabidopsis thaliana physical maps from AtDB, the
... and cosmids. Probes are defined as pieces of DNA (clones, part of a clone, or oligomers) used to identify, in whatever way, clones containing sequence common to the probe. Markers are probes that have been placed onto a genetic map. (i) Tiling path. This is defined as a hybridization-based physical ...
... and cosmids. Probes are defined as pieces of DNA (clones, part of a clone, or oligomers) used to identify, in whatever way, clones containing sequence common to the probe. Markers are probes that have been placed onto a genetic map. (i) Tiling path. This is defined as a hybridization-based physical ...
Molecular Inversion Probe
Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping as well as for studying gene copy alterationsand characteristics of specific genomic loci to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.