TEL Gene Is Involved in Myelodysplastic Syndromes
... The t(5;12) was recently cloned by Golub et al." The investicase of RA with monocytosis and fibrosis. Altogether, data gators showed that the translocation results in expression of a from the literature, from our patients no. 1, 2, and 3, and fusion transcript between a novel gene at 12~13,called TE ...
... The t(5;12) was recently cloned by Golub et al." The investicase of RA with monocytosis and fibrosis. Altogether, data gators showed that the translocation results in expression of a from the literature, from our patients no. 1, 2, and 3, and fusion transcript between a novel gene at 12~13,called TE ...
Isolation of the b-tubulin Gene From Yeast and Demonstration of its Essential Function in vivo.
... hybridize to the chick tubulin cDNA probes were identified by gel transfer hybridization (Southern, 1975). Despite the several Eco RI fragments found in total yeast DNA (Figure 1) with the chicken ol-tubulin cDNA probe (including ones 4.5 and 9 kb in length), among the recombinant X phages made with ...
... hybridize to the chick tubulin cDNA probes were identified by gel transfer hybridization (Southern, 1975). Despite the several Eco RI fragments found in total yeast DNA (Figure 1) with the chicken ol-tubulin cDNA probe (including ones 4.5 and 9 kb in length), among the recombinant X phages made with ...
Degenerate PCR - Yale School of Medicine
... Using inosine in the primers requires that the DNA polymerase used in the PCR reaction be capable of synthesizing DNA over an inosine-containing template. Taq polymerase is capable of doing this, but some others (e.g. Vent) appear not to be able to. 3) using multiple separate oligo pools at a single ...
... Using inosine in the primers requires that the DNA polymerase used in the PCR reaction be capable of synthesizing DNA over an inosine-containing template. Taq polymerase is capable of doing this, but some others (e.g. Vent) appear not to be able to. 3) using multiple separate oligo pools at a single ...
Carcinoembryonic Antigens - The Journal of Cell Biology
... and by the sequence relationships detailed in Table I. As is typical for other CEA isoantigens, the TM1-CEA polypeptide initiates with a M-amino acid leader sequence that ends at a short side-chain amino acid, alanine. The proposed NH2-terminus of TM1-CEA contains glutamine as the first residue that ...
... and by the sequence relationships detailed in Table I. As is typical for other CEA isoantigens, the TM1-CEA polypeptide initiates with a M-amino acid leader sequence that ends at a short side-chain amino acid, alanine. The proposed NH2-terminus of TM1-CEA contains glutamine as the first residue that ...
Resolving Individuals Contributing Trace Amounts of DNA to Highly
... components of the mixture [8]. Nevertheless, these methods based on STRs expectedly suffer from limited power when using severely degraded DNA [8,9]. Mitochondrial DNA (mtDNA) based on hypervariable region sequencing is useful when analyzing degraded DNA due to its high copy number and improved stab ...
... components of the mixture [8]. Nevertheless, these methods based on STRs expectedly suffer from limited power when using severely degraded DNA [8,9]. Mitochondrial DNA (mtDNA) based on hypervariable region sequencing is useful when analyzing degraded DNA due to its high copy number and improved stab ...
Pedigree Genotyping - Wageningen UR E
... hampers their full exploitation in commercial breeding programs. These markers have usually been identified in one single cross. Consequently, only one or two favourable alleles of the related QTL are identified and exploitable for markerassisted breeding (MAB), whereas a breeding program may includ ...
... hampers their full exploitation in commercial breeding programs. These markers have usually been identified in one single cross. Consequently, only one or two favourable alleles of the related QTL are identified and exploitable for markerassisted breeding (MAB), whereas a breeding program may includ ...
presentation - Genome-to-Genome Distance Calculator
... Techniques are cumbersome and cannot easily be made reproducible; different methods yield different results ...
... Techniques are cumbersome and cannot easily be made reproducible; different methods yield different results ...
Consistency analysis of redundant probe sets on Affymetrix three
... and these arrays continue to be used in individual labs as well as larger-scale projects, such as the Cancer Genome Atlas [1]. Exon arrays were developed more recently and have been marketed as being able to quantify changes in alternative splicing. Many groups have reported success using exon array ...
... and these arrays continue to be used in individual labs as well as larger-scale projects, such as the Cancer Genome Atlas [1]. Exon arrays were developed more recently and have been marketed as being able to quantify changes in alternative splicing. Many groups have reported success using exon array ...
Transcripts of the MHM region on the chicken Z chromosome
... of chickens, consists of approximately 210 tandem repeats of a BamHI 2.2-kb sequence unit. Cytosines of the CpG dinucleotides of this region are extensively methylated on the two Z chromosomes in the male but much less methylated on the single Z chromosome in the female. The state of methylation of ...
... of chickens, consists of approximately 210 tandem repeats of a BamHI 2.2-kb sequence unit. Cytosines of the CpG dinucleotides of this region are extensively methylated on the two Z chromosomes in the male but much less methylated on the single Z chromosome in the female. The state of methylation of ...
Putrescine oxidase of Micrococcus rubens : primary
... exonuclease 111 and S1 nuclease. The trimmed ends were filled-in with Klenow fragment and recircularized with T4 DNA ligase. The nucleotide sequences of each of the junctions were then determined by the dideoxynucleotide method. Nucleotide sequencing. For nucleotide sequencing, specific restriction ...
... exonuclease 111 and S1 nuclease. The trimmed ends were filled-in with Klenow fragment and recircularized with T4 DNA ligase. The nucleotide sequences of each of the junctions were then determined by the dideoxynucleotide method. Nucleotide sequencing. For nucleotide sequencing, specific restriction ...
Effects of Natural Selection on Interpopulation Divergence
... candidate loci in disease-association studies. Furthermore, interpopulation divergence may provide evidence not only of purifying selection but also of positive selection leading to allele frequency divergence between populations. To exploit information on interpopulation divergence as a source of i ...
... candidate loci in disease-association studies. Furthermore, interpopulation divergence may provide evidence not only of purifying selection but also of positive selection leading to allele frequency divergence between populations. To exploit information on interpopulation divergence as a source of i ...
Pharmacogenomics: Analyzing SNPs in the CYP2D6 Gene Using
... important to have confidence in that foundation. However, it is essentially impossible to have confidence in any phylogenetic tree using population data because of its sequence conservation. Thus, TreeSAAP would normally not be ideal for such a study since any analyses based upon a tree produced by ...
... important to have confidence in that foundation. However, it is essentially impossible to have confidence in any phylogenetic tree using population data because of its sequence conservation. Thus, TreeSAAP would normally not be ideal for such a study since any analyses based upon a tree produced by ...
Paper I- Discussion Points
... cells to observe both the replication factory (green) and the DNA locus (cyan or red) as they go through the cell cycle. We will follow the intensity of the cyan dot, and plot it as a function of time. At the time the dot replicates the intensity will double, and our microscopy is sensitive enough t ...
... cells to observe both the replication factory (green) and the DNA locus (cyan or red) as they go through the cell cycle. We will follow the intensity of the cyan dot, and plot it as a function of time. At the time the dot replicates the intensity will double, and our microscopy is sensitive enough t ...
Archives of Microbiology 167:
... showed similar restriction patterns in Southern hybridizations. We attempted to clone several larger genomic fragments (5.5-12 kb) from cosmid pSCR213 into pBluescript and/or pACYC177 without success. Most clones analyzed from these ligations were either smaller or larger than predicted and did not ...
... showed similar restriction patterns in Southern hybridizations. We attempted to clone several larger genomic fragments (5.5-12 kb) from cosmid pSCR213 into pBluescript and/or pACYC177 without success. Most clones analyzed from these ligations were either smaller or larger than predicted and did not ...
GenomeSequencing_ver3_20040929
... Partial shotgun coverage: typically 3-6X random coverage of a genome which produces sequence data of sufficient quality to enable gene identification but which is not sufficient to produce a finished genome sequence Paired reads: sequence reads determined from both ends of a cloned insert in a recom ...
... Partial shotgun coverage: typically 3-6X random coverage of a genome which produces sequence data of sufficient quality to enable gene identification but which is not sufficient to produce a finished genome sequence Paired reads: sequence reads determined from both ends of a cloned insert in a recom ...
FX 8 - ACMG
... Description of Methods: Direct DNA analysis of the CGG repeat in the fragile X mental retardation 1 gene (FMR1) was performed by Triplet Repeat Primed PCR followed by capillary electrophoresis. Southern blot analysis, when performed, uses the StB12.3 probe and genomic DNA digested with EagI/EcoRI. ...
... Description of Methods: Direct DNA analysis of the CGG repeat in the fragile X mental retardation 1 gene (FMR1) was performed by Triplet Repeat Primed PCR followed by capillary electrophoresis. Southern blot analysis, when performed, uses the StB12.3 probe and genomic DNA digested with EagI/EcoRI. ...
Hyper-eccentric structural genes in the mitochondrial genome of the
... and CONS. rF1” and “CONS. fR and CONS rF2”—to reduce the risk of biased amplification. Note that these primers were designed to outwardly anneal within the shared region that is possibly conserved among all chromosomes (see below). Each amplification consisted of 40 cycles of denaturing at 94 C fo ...
... and CONS. rF1” and “CONS. fR and CONS rF2”—to reduce the risk of biased amplification. Note that these primers were designed to outwardly anneal within the shared region that is possibly conserved among all chromosomes (see below). Each amplification consisted of 40 cycles of denaturing at 94 C fo ...
Supplementary Figures (doc 9746K)
... categories are junctions whose start and stop sites have been annotated in the Ensembl gene annotation. The only difference is that the new junction group uses different combinations of start and stop sites. The 3rd and 4th categories are junctions having new 3’ stop sites (acceptor site) or 5’ star ...
... categories are junctions whose start and stop sites have been annotated in the Ensembl gene annotation. The only difference is that the new junction group uses different combinations of start and stop sites. The 3rd and 4th categories are junctions having new 3’ stop sites (acceptor site) or 5’ star ...
Microsynth GmbH
... proven protocols and an overall experience of 25 years in the area of Sanger sequencing usually result in long reading lengths and clean sequences. Nevertheless, there are factors, which can affect the successful generation of a high quality sequence. The reasons for that are of different nature. So ...
... proven protocols and an overall experience of 25 years in the area of Sanger sequencing usually result in long reading lengths and clean sequences. Nevertheless, there are factors, which can affect the successful generation of a high quality sequence. The reasons for that are of different nature. So ...
Trouble Shooting Guide
... usually result in long reading lengths and clean sequences. Nevertheless, there are factors, which can affect the successful generation of a high quality sequence. The reasons for that are of different nature. Some causes can already be identified at the electropherogram level, while for others addi ...
... usually result in long reading lengths and clean sequences. Nevertheless, there are factors, which can affect the successful generation of a high quality sequence. The reasons for that are of different nature. Some causes can already be identified at the electropherogram level, while for others addi ...
BioinformaIcs Journal Club
... enriched in the unmapped read set. These are large regions which are either absent from the reference genome or show high divergence to the corresponding reference sequence such that each of the read ...
... enriched in the unmapped read set. These are large regions which are either absent from the reference genome or show high divergence to the corresponding reference sequence such that each of the read ...
Automated Constraint-Based Nucleotide Sequence Selection for
... selection of nucleotide sequences. This tool incorporates domain knowledge that has proven to be important in our experimental process. However, we have also formulated a framework for systematically solving a general nucleotide selection problem and have produced the program scan to assist in the s ...
... selection of nucleotide sequences. This tool incorporates domain knowledge that has proven to be important in our experimental process. However, we have also formulated a framework for systematically solving a general nucleotide selection problem and have produced the program scan to assist in the s ...
Molecular Inversion Probe
Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping as well as for studying gene copy alterationsand characteristics of specific genomic loci to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.