An economic method for the fluorescent labeling of PCR fragments
... DNA quality was bad, thus resulting in low signal strength and an artificial bump in front of the signal proper. The signals, however, can still be clearly outlined and show the expected morphology. This experiment was controlled with a conventionally labeled forward primer (data not shown). The res ...
... DNA quality was bad, thus resulting in low signal strength and an artificial bump in front of the signal proper. The signals, however, can still be clearly outlined and show the expected morphology. This experiment was controlled with a conventionally labeled forward primer (data not shown). The res ...
A New Sequencing Primer and Workflow Increase 5` Resolution and
... quality readable bases by base 25 after the sequencing primer while the new primers produced high quality bases by base 5, and by base 1 in many cases. Because of improved resolution, basecalling accuracy was increased. This simplified process without a separate PCR clean-up step reduced the overall ...
... quality readable bases by base 25 after the sequencing primer while the new primers produced high quality bases by base 5, and by base 1 in many cases. Because of improved resolution, basecalling accuracy was increased. This simplified process without a separate PCR clean-up step reduced the overall ...
How to measure chromatin modifications
... One of the most exciting recent advances in technologies for studying epigenetic phenomena at a genomic scale relies on the combination of ChIP experiments with highthroughput sequencing. The procedure that is outlined here is specific to the Illumina Genome Analyzer using Solexa technology, althoug ...
... One of the most exciting recent advances in technologies for studying epigenetic phenomena at a genomic scale relies on the combination of ChIP experiments with highthroughput sequencing. The procedure that is outlined here is specific to the Illumina Genome Analyzer using Solexa technology, althoug ...
clinchem.org - Clinical Chemistry
... 3500 newborn males (8 ) and is inherited in an X-linked recessive pattern, resulting from variations in the DMD gene on Xp21.1. The DMD gene, consisting of 79 exons and spanning a region of 2.4 million bp of genomic DNA, is the largest known human gene (9, 10 ). Approximately 55%– 65% of DMD cases a ...
... 3500 newborn males (8 ) and is inherited in an X-linked recessive pattern, resulting from variations in the DMD gene on Xp21.1. The DMD gene, consisting of 79 exons and spanning a region of 2.4 million bp of genomic DNA, is the largest known human gene (9, 10 ). Approximately 55%– 65% of DMD cases a ...
Parental Genome Separation and Elimination of Cells and
... When the O. violaceus genomic probe was applied to O. violaceus preparations, signals distributed along the whole length of chromosomes (Fig. 1A1 and A2). In contrast, GISH in Brassica was normally characterized by strong signals at centromeric heterochromatin and only very weak hybridization on chr ...
... When the O. violaceus genomic probe was applied to O. violaceus preparations, signals distributed along the whole length of chromosomes (Fig. 1A1 and A2). In contrast, GISH in Brassica was normally characterized by strong signals at centromeric heterochromatin and only very weak hybridization on chr ...
Direct Deletion Analysis in Two Duchenne Muscular Dystrophy
... 07A, 5’-7n4, STR 44, STR 45, STR 49, and STR 50. The PCR products of the alleles were analyzed by superimposing the traces of the proband to that of her relatives. The haplotypes of the allele lengths at these loci were determined in both cases by assigning arbitrary letters to each allelic variatio ...
... 07A, 5’-7n4, STR 44, STR 45, STR 49, and STR 50. The PCR products of the alleles were analyzed by superimposing the traces of the proband to that of her relatives. The haplotypes of the allele lengths at these loci were determined in both cases by assigning arbitrary letters to each allelic variatio ...
Use of Alternative Primers for Gender Discrimination in Human
... for genotyping. The Combined DNA Identity System (CODIS) contains a core set of STR markers for human identity testing1 that are discriminatory over a wide range of ethnicities. An additional marker, amelogenin,2 has been included in the CODIS series for gender discrimination. The amelogenin primers ...
... for genotyping. The Combined DNA Identity System (CODIS) contains a core set of STR markers for human identity testing1 that are discriminatory over a wide range of ethnicities. An additional marker, amelogenin,2 has been included in the CODIS series for gender discrimination. The amelogenin primers ...
High-Resolution Single-Copy Gene Fluorescence in Situ
... High-resolution cytogenetic maps provide important biological information on genome organization and function, as they correlate genetic distance with cytological structures, and are an invaluable complement to physical sequence data. The most direct way to generate a cytogenetic map is to localize ...
... High-resolution cytogenetic maps provide important biological information on genome organization and function, as they correlate genetic distance with cytological structures, and are an invaluable complement to physical sequence data. The most direct way to generate a cytogenetic map is to localize ...
Identification of loci affecting teat number by
... Where y represents the vector of phenotypes of all tested pigs; β is the fixed effects, including all grand mean and sex; α is the regression coefficient of substituting allele; u is the vector of polygenic effect; e is the vector of residual errors. X and Z are incidence matrices for β and u, res ...
... Where y represents the vector of phenotypes of all tested pigs; β is the fixed effects, including all grand mean and sex; α is the regression coefficient of substituting allele; u is the vector of polygenic effect; e is the vector of residual errors. X and Z are incidence matrices for β and u, res ...
Real time PCR and it`s role in diagnosis
... Improving our understanding of the biology of the Plasmodium falciparum parasite is of extreme importance if we are to combat human malaria.This parasite uses the process of antigenic variation to expose the human immune system to continually changing antigens on the surface of infected red blood ce ...
... Improving our understanding of the biology of the Plasmodium falciparum parasite is of extreme importance if we are to combat human malaria.This parasite uses the process of antigenic variation to expose the human immune system to continually changing antigens on the surface of infected red blood ce ...
Heterosis, epistasis and linkage disequilibrium in
... the data, is impossible. Alternative methods are used (Snedecor & Cochran, 19671, two of which necessarily involve the sacrifice of some data. First, I use unweighted class means (i.e. disregarding the size of and variance within classes). Of the 27 genotypes possible, four are missing in both sexes ...
... the data, is impossible. Alternative methods are used (Snedecor & Cochran, 19671, two of which necessarily involve the sacrifice of some data. First, I use unweighted class means (i.e. disregarding the size of and variance within classes). Of the 27 genotypes possible, four are missing in both sexes ...
Advanced Analysis with SeqMonk Version 1.3.0
... at all. The problem is that because you saw nothing you can have no idea how much more sequencing you would have had to have performed until you saw some data in that position. Comparing an empty probe to one with a small amount of data (especially when there is a difference in the total amount of d ...
... at all. The problem is that because you saw nothing you can have no idea how much more sequencing you would have had to have performed until you saw some data in that position. Comparing an empty probe to one with a small amount of data (especially when there is a difference in the total amount of d ...
08.seg_dup_els - NYU Computer Science
... Zhou-Mishra Model of Segmental Duplications Zhou-Mishra [Zhou & Mishra (2005), Zhou(2005)] model focuses on the hypothesis that recombination between homologous repeats from a family X, e.g., Alu or L1, contributes to the recent segmental duplication processes in mammalian genomes. The model reflect ...
... Zhou-Mishra Model of Segmental Duplications Zhou-Mishra [Zhou & Mishra (2005), Zhou(2005)] model focuses on the hypothesis that recombination between homologous repeats from a family X, e.g., Alu or L1, contributes to the recent segmental duplication processes in mammalian genomes. The model reflect ...
Statistical analysis of DNA microarray data
... These genes will be similarly expressed in all samples. Difficult to identify – need to be confirmed Affymetrix GeneChip provides a set of house keeping genes based on a large set of tests on different tissues and were found to have low variability in these samples (but still no guarantee). ...
... These genes will be similarly expressed in all samples. Difficult to identify – need to be confirmed Affymetrix GeneChip provides a set of house keeping genes based on a large set of tests on different tissues and were found to have low variability in these samples (but still no guarantee). ...
Pattern of diversity in the genomic region near the
... selection and diversity in a crop species. The tb1 gene is largely responsible for a major-effect quantitative trait locus on chromosome 1 that controls differences in plant architecture between maize and its wild relative, teosinte (Z. mays subsp. mexicana and subsp. parviglumis) (8–11). The tb1 ge ...
... selection and diversity in a crop species. The tb1 gene is largely responsible for a major-effect quantitative trait locus on chromosome 1 that controls differences in plant architecture between maize and its wild relative, teosinte (Z. mays subsp. mexicana and subsp. parviglumis) (8–11). The tb1 ge ...
DNA SEQUENCING (using a Li
... methods, and their derivations. Both methods were first described in 1977. The first method (Maxam and Gilbert 1977) is based on specific chemical degradation of the DNA. The DNA is first end-labeled using 35s or 33P, followed by separation of the two strands on a gel. Four aliquots of the desired s ...
... methods, and their derivations. Both methods were first described in 1977. The first method (Maxam and Gilbert 1977) is based on specific chemical degradation of the DNA. The DNA is first end-labeled using 35s or 33P, followed by separation of the two strands on a gel. Four aliquots of the desired s ...
Genomic scars as biomarkers of homologous recombination
... Figure 1 Genomic aberrations in cancer. Three classes of genomic aberration that develop in cancer cells are depicted: mutations of less than 1 Kbp in length (top box), structural copy number aberrations (CNAs) (bottom left box), and structural rearrangements (bottom right box). The initial state in ...
... Figure 1 Genomic aberrations in cancer. Three classes of genomic aberration that develop in cancer cells are depicted: mutations of less than 1 Kbp in length (top box), structural copy number aberrations (CNAs) (bottom left box), and structural rearrangements (bottom right box). The initial state in ...
What`s in a Genotype? - CEUR Workshop Proceedings
... down into reference and variant components, the genomic variation complement can be further decomposed into one or more variant single locus complements, representing the set of all complementary loci where at least one variant exists (Figure 1B). This complement is typically a pair of sequences for ...
... down into reference and variant components, the genomic variation complement can be further decomposed into one or more variant single locus complements, representing the set of all complementary loci where at least one variant exists (Figure 1B). This complement is typically a pair of sequences for ...
Case Report Section
... consistent with the recurrence and clonal evolution of the leukemic clone. Other molecular cytogenetics technics Fluorescence in situ hybridization (FISH) using LSI 4q12 tricolor and LSI ETV6/RUNX1 ES dual color DNA probes were performed (Abbott Molecular. Downers Grove, IL) on the abnormal metaphas ...
... consistent with the recurrence and clonal evolution of the leukemic clone. Other molecular cytogenetics technics Fluorescence in situ hybridization (FISH) using LSI 4q12 tricolor and LSI ETV6/RUNX1 ES dual color DNA probes were performed (Abbott Molecular. Downers Grove, IL) on the abnormal metaphas ...
PDF - Molecular Cytogenetics
... were digested with HpaII (Invitrogen, Carlsbad, CA, USA), for 2 h at 37 °C, or mock-digested. DNA genotyping was carried out in quantitative fluorescence polymerase chain biplex reactions (QF-PCR) in approximately 20 ng of digested or mock-digested DNA using 0.8 μM (AR) and 1.2 μM (RP2) of each prim ...
... were digested with HpaII (Invitrogen, Carlsbad, CA, USA), for 2 h at 37 °C, or mock-digested. DNA genotyping was carried out in quantitative fluorescence polymerase chain biplex reactions (QF-PCR) in approximately 20 ng of digested or mock-digested DNA using 0.8 μM (AR) and 1.2 μM (RP2) of each prim ...
Associative and Endophytic Nitrogen-fixing Bacteria and Cyanobacterial Association
... distributed among bacteria known until now; nif-genes could frequently be present in the unculturable / not cultured fraction of environmental bacteria. 2.3. Diversity Studies Using Molecular Probing and Fingerprinting Techniques In addition to the 16S- and 23S-rRNA oligonucleotide probes, a number ...
... distributed among bacteria known until now; nif-genes could frequently be present in the unculturable / not cultured fraction of environmental bacteria. 2.3. Diversity Studies Using Molecular Probing and Fingerprinting Techniques In addition to the 16S- and 23S-rRNA oligonucleotide probes, a number ...
Molecular Inversion Probe
Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping as well as for studying gene copy alterationsand characteristics of specific genomic loci to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.