Saccharomycopsis fibuligera and Yarrowia lipol`ica
... haloes were observed after growth on YPSS for 2 d. Colonies of the intergeneric hybrid between A6 and Y. lipobtica 21501-4B also showed a clear halo around the colony on YPSS. On this medium, colonies of Y. lipobtica 21501-4B did not show a halo; this result was expected as Y. lipobtica is not amylo ...
... haloes were observed after growth on YPSS for 2 d. Colonies of the intergeneric hybrid between A6 and Y. lipobtica 21501-4B also showed a clear halo around the colony on YPSS. On this medium, colonies of Y. lipobtica 21501-4B did not show a halo; this result was expected as Y. lipobtica is not amylo ...
Fulltext PDF - Indian Academy of Sciences
... translocations (Fedak and Han 2005; Li et al. 2008; Li and Wang 2009). But there had no reports about the reduced height gene introduced from Th. ponticum. We had developed an addition line 31504, with reduced plant height than its wheat parent, from the cross between wheat cultivar Lumai 5 and whea ...
... translocations (Fedak and Han 2005; Li et al. 2008; Li and Wang 2009). But there had no reports about the reduced height gene introduced from Th. ponticum. We had developed an addition line 31504, with reduced plant height than its wheat parent, from the cross between wheat cultivar Lumai 5 and whea ...
DNA breathing dynamics distinguish binding from nonbinding
... versus three). The second term of the Hamiltonian represents the part of the stacking interactions between two consecutive nucleotides that influence the transverse stretching motion. The phenomenological parameters and , were introduced for the first time in (12) to fulfill the requirement for a sh ...
... versus three). The second term of the Hamiltonian represents the part of the stacking interactions between two consecutive nucleotides that influence the transverse stretching motion. The phenomenological parameters and , were introduced for the first time in (12) to fulfill the requirement for a sh ...
Relatedness in the post-genomic era: is it still
... lineage paths in pedigrees (BOX 1) provides a mathematically beautiful structure that has historically been useful, but its weaknesses are apparent. Pedigree founders are typically assumed to be unrelated, but this is only realistic in certain settings, such as some designed breeding programmes or a ...
... lineage paths in pedigrees (BOX 1) provides a mathematically beautiful structure that has historically been useful, but its weaknesses are apparent. Pedigree founders are typically assumed to be unrelated, but this is only realistic in certain settings, such as some designed breeding programmes or a ...
Convergent Evolution in the Genetic Basis of Müllerian Mimicry in
... (10:0.1, pH 8.0). AFLP templates were prepared for 35 H. melpomene brood 44 progeny, F1 parents, and grandparents using half reactions of AFLP analysis system II (Invitrogen Life Technologies) according to manufacturer’s instructions, with EcoRI and MseI restriction enzymes. AFLP PCR reactions were ...
... (10:0.1, pH 8.0). AFLP templates were prepared for 35 H. melpomene brood 44 progeny, F1 parents, and grandparents using half reactions of AFLP analysis system II (Invitrogen Life Technologies) according to manufacturer’s instructions, with EcoRI and MseI restriction enzymes. AFLP PCR reactions were ...
Convergent Evolution in the Genetic Basis of Müllerian
... (10:0.1, pH 8.0). AFLP templates were prepared for 35 H. melpomene brood 44 progeny, F1 parents, and grandparents using half reactions of AFLP analysis system II (Invitrogen Life Technologies) according to manufacturer’s instructions, with EcoRI and MseI restriction enzymes. AFLP PCR reactions were ...
... (10:0.1, pH 8.0). AFLP templates were prepared for 35 H. melpomene brood 44 progeny, F1 parents, and grandparents using half reactions of AFLP analysis system II (Invitrogen Life Technologies) according to manufacturer’s instructions, with EcoRI and MseI restriction enzymes. AFLP PCR reactions were ...
selective genotyping and phenotyping strategies in a complex trait
... and a loss of information due to censoring, or to follow up the entire sample until all events have been observed, which implies greater cost but a minimal loss of information. As far as we are aware, these tradeoffs have not been studied. In studies where phenotyping is more expensive than genotypi ...
... and a loss of information due to censoring, or to follow up the entire sample until all events have been observed, which implies greater cost but a minimal loss of information. As far as we are aware, these tradeoffs have not been studied. In studies where phenotyping is more expensive than genotypi ...
Cytogenetics
... Results from errors in division during meiosis, where a daughter cell receives both pairs of a particular chromosome (nondisjunction errors). Addition of an extra chromosome, trisomy, has been described for all the chromosomes but only three autosomal trisomies survive to birth. Those are trisomies ...
... Results from errors in division during meiosis, where a daughter cell receives both pairs of a particular chromosome (nondisjunction errors). Addition of an extra chromosome, trisomy, has been described for all the chromosomes but only three autosomal trisomies survive to birth. Those are trisomies ...
TaqMan-Based Real-Time PCR for Genotyping Common
... corresponding reports of negative associations with disease (2 ). Thus, development of a simple, highthroughput HP genotyping method is needed to facilitate these association studies. Several methods for phenotyping HP1 and HP2 have been described. High-pressure gel-permeation chromatography and ge ...
... corresponding reports of negative associations with disease (2 ). Thus, development of a simple, highthroughput HP genotyping method is needed to facilitate these association studies. Several methods for phenotyping HP1 and HP2 have been described. High-pressure gel-permeation chromatography and ge ...
MONSTER Documentation
... variants may be included in the joint test, and the variants may come from, e.g., sequence or genotype data. In particular, MONSTER is suitable for testing for association between a trait and a set of rare variants. MONSTER performs a rapid and robust test against the null hypothesis that none of th ...
... variants may be included in the joint test, and the variants may come from, e.g., sequence or genotype data. In particular, MONSTER is suitable for testing for association between a trait and a set of rare variants. MONSTER performs a rapid and robust test against the null hypothesis that none of th ...
SNP-Based Mapping of Crossover Recombination in
... plifying scoring. In addition, multiple markers can be followed in a single cross (limited only by the number of PCRs one can carry out on the DNA sample obtained). A subset of these poly morphisms alter (create or destroy) cleavage sites for restriction endonucleases. Such polymorphisms, referred ...
... plifying scoring. In addition, multiple markers can be followed in a single cross (limited only by the number of PCRs one can carry out on the DNA sample obtained). A subset of these poly morphisms alter (create or destroy) cleavage sites for restriction endonucleases. Such polymorphisms, referred ...
TaqMan® SNP Genotyping Assays User Guide
... particular level of observation or actions as described below: Note: Provides information that may be of interest or help but is not critical to the use of the product. ...
... particular level of observation or actions as described below: Note: Provides information that may be of interest or help but is not critical to the use of the product. ...
The geography of introgression in a patchy environment and the
... enough to impede the flow of unlinked neutral genes, it has reached a “congealed” state so that a collection of unlinked loci under concordant, moderate, selection would roughly behave as a single superlocus under strong selection because strong linkage disequilibrium is maintained between them (Bar ...
... enough to impede the flow of unlinked neutral genes, it has reached a “congealed” state so that a collection of unlinked loci under concordant, moderate, selection would roughly behave as a single superlocus under strong selection because strong linkage disequilibrium is maintained between them (Bar ...
The geography of introgression in a patchy
... enough to impede the flow of unlinked neutral genes, it has reached a “congealed” state so that a collection of unlinked loci under concordant, moderate, selection would roughly behave as a single superlocus under strong selection because strong linkage disequilibrium is maintained between them (Bar ...
... enough to impede the flow of unlinked neutral genes, it has reached a “congealed” state so that a collection of unlinked loci under concordant, moderate, selection would roughly behave as a single superlocus under strong selection because strong linkage disequilibrium is maintained between them (Bar ...
Post-PCR sterilization: a method to control carryover contamination
... cloning techniques, the creation of allele-specific hybridization protocols and, most importantly, template-dependent polymerase extension reactions were developed into nucleic acid amplification protocols by using the oligonucleotides as specific primers (1 - 4 ) . The first of these procedures, th ...
... cloning techniques, the creation of allele-specific hybridization protocols and, most importantly, template-dependent polymerase extension reactions were developed into nucleic acid amplification protocols by using the oligonucleotides as specific primers (1 - 4 ) . The first of these procedures, th ...
Microbial Ecology: Where are we now?
... DNA, using either primers or probes each of which have been demonstrated to have unique NGS application. Target enrichment techniques are evaluated in terms of multiplexity, specificity, uniformity, drop-off rate and reproducibility. The above-mentioned techniques were all evaluated using these key ...
... DNA, using either primers or probes each of which have been demonstrated to have unique NGS application. Target enrichment techniques are evaluated in terms of multiplexity, specificity, uniformity, drop-off rate and reproducibility. The above-mentioned techniques were all evaluated using these key ...
INFINITI CYP450 2C19+ Assay
... Genetic polymorphisms in CYP2C19 are common and can affect therapeutic response to drugs. The enzyme activity is expressed at highly variable levels. Three phenotypes are identified: poor metabolizers (PM), intermediate metabolizers (IM) and normal metabolizers (NM). CYP2C19 acts on 5-10% of drugs i ...
... Genetic polymorphisms in CYP2C19 are common and can affect therapeutic response to drugs. The enzyme activity is expressed at highly variable levels. Three phenotypes are identified: poor metabolizers (PM), intermediate metabolizers (IM) and normal metabolizers (NM). CYP2C19 acts on 5-10% of drugs i ...
pdf
... for only 2.0 to 2.6% of the relative fluorescence signal, suggesting that the abundance of this gammaproteobacterial AOB were sufficiently low and uniform that their omission from the qPCR amplification does not change the observed trends. By contrast, in the Vineyard Sound coral experiment, AOA wer ...
... for only 2.0 to 2.6% of the relative fluorescence signal, suggesting that the abundance of this gammaproteobacterial AOB were sufficiently low and uniform that their omission from the qPCR amplification does not change the observed trends. By contrast, in the Vineyard Sound coral experiment, AOA wer ...
Real-time PCR Handbook
... can withstand is typically used (usually 95°C). The denaturation time can be increased if template GC content is high. 2. Annealing: During annealing, complementary sequences have an opportunity to hybridize, so an appropriate temperature is used that is based on the calculated melting temperature ...
... can withstand is typically used (usually 95°C). The denaturation time can be increased if template GC content is high. 2. Annealing: During annealing, complementary sequences have an opportunity to hybridize, so an appropriate temperature is used that is based on the calculated melting temperature ...
Characterization of the unique intron
... acid sequence and their genes often contain multiple introns. Euglena gracilis, a unicellular protist, has a LHCPII precursor (pLHCPII) that is a polyprotein containing multiple copies of LHCPII covalently joined by a conserved decapeptide linker (3-5). The amino acid sequence of Euglena pLHCPII was ...
... acid sequence and their genes often contain multiple introns. Euglena gracilis, a unicellular protist, has a LHCPII precursor (pLHCPII) that is a polyprotein containing multiple copies of LHCPII covalently joined by a conserved decapeptide linker (3-5). The amino acid sequence of Euglena pLHCPII was ...
Rapid Microscope Based Identification Method for Tuberculosis and
... identification of different species of microorganisms without prior cultivation. This technique (oligonucleotide FISH) was then further developed and applied by Amann et al in the early 1990s on a variety of environmental samples [30]. Some of the first oligonucleotide FISH applications on medical s ...
... identification of different species of microorganisms without prior cultivation. This technique (oligonucleotide FISH) was then further developed and applied by Amann et al in the early 1990s on a variety of environmental samples [30]. Some of the first oligonucleotide FISH applications on medical s ...
Genetic Analysis of HNF4A Polymorphisms in Caucasian
... equilibrium and to calculate pairwise linkage disequilibrium (LD) statistics. Within the regions of strongest LD, HAPLO.SCORE (25 and http://www.mayo. edu/statgen/) and Dandelion (26) were used to estimate haplotypes and to test for association of these haplotypes within the case-control population. ...
... equilibrium and to calculate pairwise linkage disequilibrium (LD) statistics. Within the regions of strongest LD, HAPLO.SCORE (25 and http://www.mayo. edu/statgen/) and Dandelion (26) were used to estimate haplotypes and to test for association of these haplotypes within the case-control population. ...
Haplotypes at LBX1 Have Distinct Inheritance Patterns with
... After visual inspection of the genotype clusters of the top 500 associated SNPs, we identified rs11190878 (Ptrend = 4.18×10–9, OR = 0.63) in the LBX1 locus as associated with AIS (Fig. C in S2 File). None of the other genotyped markers reached the genome-wide significance threshold (P<5×10–8). Resul ...
... After visual inspection of the genotype clusters of the top 500 associated SNPs, we identified rs11190878 (Ptrend = 4.18×10–9, OR = 0.63) in the LBX1 locus as associated with AIS (Fig. C in S2 File). None of the other genotyped markers reached the genome-wide significance threshold (P<5×10–8). Resul ...
Engineered Cpf1 Enzymes with Altered PAM Specificities
... Inhibitor Cocktail (Roche). T7-driven crRNA was transcribed in vitro using custom oligonucleotides and HiScribe T7 in vitro Transcription Kit (NEB) following the manufacturer’s recommended protocol. For the PAM library, a degenerate 8 bp sequence preceding a 33 bp target site1 was cloned into the MC ...
... Inhibitor Cocktail (Roche). T7-driven crRNA was transcribed in vitro using custom oligonucleotides and HiScribe T7 in vitro Transcription Kit (NEB) following the manufacturer’s recommended protocol. For the PAM library, a degenerate 8 bp sequence preceding a 33 bp target site1 was cloned into the MC ...
exon junctions of Euglena gene(s) - DigitalCommons@University of
... containing 7.4 kb of the 3' end of a LHCPII gene (4). Nine exons were identified in this clone based on sequence homology with Arabidopsis LHCPII. They can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of an LHCPII, followed by 4 complete LHCPIIs (4). I ...
... containing 7.4 kb of the 3' end of a LHCPII gene (4). Nine exons were identified in this clone based on sequence homology with Arabidopsis LHCPII. They can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of an LHCPII, followed by 4 complete LHCPIIs (4). I ...
Molecular Inversion Probe
Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping as well as for studying gene copy alterationsand characteristics of specific genomic loci to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.