Chemical and organic fertilizers affect physiological performance
... hundreds of host plants. In the current study, zinc sulfate and vermicompost as chemical and organic fertilizers, were added into cultural soil of Capsicum annuum to determine their effects on physiology and antioxidant activities of M. persicae. The aphids reared on zinc sulfate-treated culture sho ...
... hundreds of host plants. In the current study, zinc sulfate and vermicompost as chemical and organic fertilizers, were added into cultural soil of Capsicum annuum to determine their effects on physiology and antioxidant activities of M. persicae. The aphids reared on zinc sulfate-treated culture sho ...
Amplification of 16S rRNA Genes from Frankia Strains in Root
... ranged from 0 to 5 CFU on LB agar and R2A agar per 104 hyphal clusters. Each hyphal cluster contained between 102 and 103 Frankia genomes, so the measurable proportion of contaminating bacterial genomes was quite low. Phenolic compounds present in actinorhizal nodule homogenates normally turn bright ...
... ranged from 0 to 5 CFU on LB agar and R2A agar per 104 hyphal clusters. Each hyphal cluster contained between 102 and 103 Frankia genomes, so the measurable proportion of contaminating bacterial genomes was quite low. Phenolic compounds present in actinorhizal nodule homogenates normally turn bright ...
Eliminating Perinatal HIV Transmission
... perinatal period, perinatal HIV transmission is usually preventable in all but 2% or less cases ...
... perinatal period, perinatal HIV transmission is usually preventable in all but 2% or less cases ...
No Slide Title
... SHORT TANDEM REPEATS • Easy to amplify • Both heterozygote alleles amplify well • Number of repeats highly variable • Good for identification ...
... SHORT TANDEM REPEATS • Easy to amplify • Both heterozygote alleles amplify well • Number of repeats highly variable • Good for identification ...
Electrophoresis Revised
... Liquid is never drawn into the barrel of the micropipette itself. An appropriate tip should always be placed firmly on the end. Since the principle by which the micropipette works is the creation of a vacuum in the tip, causing liquid to be drawn up, it is critical that the tip be on tight enough to ...
... Liquid is never drawn into the barrel of the micropipette itself. An appropriate tip should always be placed firmly on the end. Since the principle by which the micropipette works is the creation of a vacuum in the tip, causing liquid to be drawn up, it is critical that the tip be on tight enough to ...
Mutual Interactions of the Phosphate Groups in Locally Deformed
... (Borah et al. 1985; Nishimura et al. 1986; Sarma et al. 1986). It is also worth of being noticed that at 0.7 mol/l NaCl the backbones of both B- and A-DNA tend to overwind, presumably to counteract base stacking tendencies to unwind the double helices (Šponer and Kypr 1989). At high-salt, even the i ...
... (Borah et al. 1985; Nishimura et al. 1986; Sarma et al. 1986). It is also worth of being noticed that at 0.7 mol/l NaCl the backbones of both B- and A-DNA tend to overwind, presumably to counteract base stacking tendencies to unwind the double helices (Šponer and Kypr 1989). At high-salt, even the i ...
Use of a novel cassette to label phenotypically a cryptic plasmid of
... suitable dilution to give approximately 100 colonies per plate. Incubation at 37 "C was continued until colonies were 2-3 mm in diameter, a t which stage the plates were sprayed with a 0.5 M-catechol solution (Zukowski et al., 1983). After 5 min the colonies were scored XylE+or X,ylE-. A selection o ...
... suitable dilution to give approximately 100 colonies per plate. Incubation at 37 "C was continued until colonies were 2-3 mm in diameter, a t which stage the plates were sprayed with a 0.5 M-catechol solution (Zukowski et al., 1983). After 5 min the colonies were scored XylE+or X,ylE-. A selection o ...
draft dna profiling bill 2007
... administration of justice, analysis of DNA found at the scene of crime, of the victim or offender has been used to establish identity. The DNA analysis offers sensitive information which, if, misused can cause harm to person or society. There is, thus, need to regulate the use of DNA Profiles throug ...
... administration of justice, analysis of DNA found at the scene of crime, of the victim or offender has been used to establish identity. The DNA analysis offers sensitive information which, if, misused can cause harm to person or society. There is, thus, need to regulate the use of DNA Profiles throug ...
Transposon stability and a role for conjugational transfer in adaptive mutability
... presence of the inducer Tet (13). For the episomal allele, the analysis was carried in the presence of streptomycin (Sm), conditions in which conjugal transfer to scavenger cells is required for detection of reversion (10), because the lacI33carrying cells are sensitive to Sm and the scavenger cells ...
... presence of the inducer Tet (13). For the episomal allele, the analysis was carried in the presence of streptomycin (Sm), conditions in which conjugal transfer to scavenger cells is required for detection of reversion (10), because the lacI33carrying cells are sensitive to Sm and the scavenger cells ...
Part III: Laboratory – Electrophoresis
... frequencies of recombination between the gene of interest and the genes previously mapped allow the gene of interest to be placed on the map. However, markers for genetic mapping don’t necessarily have to be mutations that cause phenotypic changes. They can also be variations in DNA sequences that a ...
... frequencies of recombination between the gene of interest and the genes previously mapped allow the gene of interest to be placed on the map. However, markers for genetic mapping don’t necessarily have to be mutations that cause phenotypic changes. They can also be variations in DNA sequences that a ...
The Rad50 Signature Motif: Essential to ATP Binding and
... The repair of double-strand breaks in DNA is an essential process in all organisms, and requires the coordinated activities of evolutionarily conserved protein assemblies. One of the most critical of these is the Mre11/ Rad50 (M/R) complex, which is present in all three biological kingdoms, but is n ...
... The repair of double-strand breaks in DNA is an essential process in all organisms, and requires the coordinated activities of evolutionarily conserved protein assemblies. One of the most critical of these is the Mre11/ Rad50 (M/R) complex, which is present in all three biological kingdoms, but is n ...
Rapid and accurate cancer somatic mutation
... sample quality. In application examples, the PCR-based mutation detection results are consistent with Pyrosequencing results for the same samples. The qBiomarker Somatic Mutation PCR Arrays, combining laboratory-verified assays, comprehensive content, and integrated data analysis software, are highl ...
... sample quality. In application examples, the PCR-based mutation detection results are consistent with Pyrosequencing results for the same samples. The qBiomarker Somatic Mutation PCR Arrays, combining laboratory-verified assays, comprehensive content, and integrated data analysis software, are highl ...
The Role of DNA-PKcs and Artemis in Opening Viral DNA
... DNA hairpin structures in the classical nonhomologous endjoining (NHEJ) repair pathway (26). The Rag proteins also have some roles in opening hairpin-coding ends in V(D)J recombination (13). Although our understanding of the metabolism of DNA hairpin structures at cellular DNA DSBs has progressed, l ...
... DNA hairpin structures in the classical nonhomologous endjoining (NHEJ) repair pathway (26). The Rag proteins also have some roles in opening hairpin-coding ends in V(D)J recombination (13). Although our understanding of the metabolism of DNA hairpin structures at cellular DNA DSBs has progressed, l ...
Recombinant DNA technology and molecular cloning
... can be isolated and amplified. One method of isolation and amplification of a gene of interest is to clone the gene by inserting it into another DNA molecule that serves as a vehicle or vector that can be replicated in living cells. When these two DNAs of different origin are combined, the result is ...
... can be isolated and amplified. One method of isolation and amplification of a gene of interest is to clone the gene by inserting it into another DNA molecule that serves as a vehicle or vector that can be replicated in living cells. When these two DNAs of different origin are combined, the result is ...
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides
... This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal lattice, with the packing shown in Figure 1a. The conformation of a sin ...
... This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal lattice, with the packing shown in Figure 1a. The conformation of a sin ...
3D DNA Crystals and Nanotechnology
... origami, and DNA brick methods have clearly demonstrated the power of DNA as a self-assembling construction material, but they have also shifted emphasis from the initial motivations of the field. Further, there are several significant differences between these types of nanoscale assemblies of large ...
... origami, and DNA brick methods have clearly demonstrated the power of DNA as a self-assembling construction material, but they have also shifted emphasis from the initial motivations of the field. Further, there are several significant differences between these types of nanoscale assemblies of large ...
The biased nucleotide composition of the HIV genome: a constant
... The RNA genomes of HIV-1 group M virus isolates contain a similar amount of A-nucleotides as those of group O (35%, Table 1). Group N and P viruses appear to contain slightly higher (group N) or lower (group P) levels of A-nucleotides, but only one (group P) or no (group N) full-length genomes with ...
... The RNA genomes of HIV-1 group M virus isolates contain a similar amount of A-nucleotides as those of group O (35%, Table 1). Group N and P viruses appear to contain slightly higher (group N) or lower (group P) levels of A-nucleotides, but only one (group P) or no (group N) full-length genomes with ...
The genomic landscape of meiotic crossovers and gene
... chromosomes generally form pairs, with each chromosome in the pair containing the same number of genes. The genes also come in the same order, but the exact sequence of DNA bases within the genes can be different. When sex cells—such as egg, sperm or pollen cells—are made, each pair of chromosomes i ...
... chromosomes generally form pairs, with each chromosome in the pair containing the same number of genes. The genes also come in the same order, but the exact sequence of DNA bases within the genes can be different. When sex cells—such as egg, sperm or pollen cells—are made, each pair of chromosomes i ...
Industrial Zinc Plating Processes
... The metal deposit distribution is affected by the variation of the cathode efficiency with current density. In plating solutions in which the cathode efficiencies decrease rapidly as current density increases, excess deposits will plate on edges and corners. This phenomenon is coined throwing power. ...
... The metal deposit distribution is affected by the variation of the cathode efficiency with current density. In plating solutions in which the cathode efficiencies decrease rapidly as current density increases, excess deposits will plate on edges and corners. This phenomenon is coined throwing power. ...
Genetic Polymorphism of Human CYP2E1
... To evaluate the functional importance of the mutations in the CYP2E1*2 and CYP2E1*3 alleles, site-directed mutagenesis was used to introduce these mutations into the wild-type CYP2E1 cDNA. The cDNAs were inserted into the pCMV4 expression vector and subsequently expressed in COS-1 cells. For compari ...
... To evaluate the functional importance of the mutations in the CYP2E1*2 and CYP2E1*3 alleles, site-directed mutagenesis was used to introduce these mutations into the wild-type CYP2E1 cDNA. The cDNAs were inserted into the pCMV4 expression vector and subsequently expressed in COS-1 cells. For compari ...
Generalized Transduction by Phage P22 in Salmonella typhimurium. II. Mechanisms of Integration of Transducing DNA.
... in this experiment the density of the his+ particles can be calculated from the known densities of the phage (p = 1.506 g/cm3) and the pur+ transducing particles (p = l-511 g/om3). The average density of the his+ transducing particles in this gradient is calculated to be approximately l-512 g/cm3 (a ...
... in this experiment the density of the his+ particles can be calculated from the known densities of the phage (p = 1.506 g/cm3) and the pur+ transducing particles (p = l-511 g/om3). The average density of the his+ transducing particles in this gradient is calculated to be approximately l-512 g/cm3 (a ...
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... carried out in pUCl8. To do so, pUCl8 was linearized with Hindlll and then partially cut with HaeII. The ...
... carried out in pUCl8. To do so, pUCl8 was linearized with Hindlll and then partially cut with HaeII. The ...
Visual gene developer: a fully programmable bioinformatics
... cannot be guaranteed, particularly in diverse host organisms. Therefore, there is a need to develop flexible software that offers a greater variability of gene optimization strategies as well as modeling tools, and the ability to couple experimental studies with algorithm development. At the same ti ...
... cannot be guaranteed, particularly in diverse host organisms. Therefore, there is a need to develop flexible software that offers a greater variability of gene optimization strategies as well as modeling tools, and the ability to couple experimental studies with algorithm development. At the same ti ...
A large scale analysis of resistance gene
... A. thaliana (eg. Simonich and Innes 1995; Gassmann et al. 1999; Deslandes et al. 2002) and in several other plant species (eg. Lawrence et al. 1995; Thomas et al. 1997; Milligan et al. 1998; Wang et al. 1999). R-genes can be divided into families based upon homologous domains in their protein produc ...
... A. thaliana (eg. Simonich and Innes 1995; Gassmann et al. 1999; Deslandes et al. 2002) and in several other plant species (eg. Lawrence et al. 1995; Thomas et al. 1997; Milligan et al. 1998; Wang et al. 1999). R-genes can be divided into families based upon homologous domains in their protein produc ...
- Philsci-Archive
... molecular biologists. Moreover, in the philosophical literature there are discussions of closely related ideas, although the connections with causal notions of biological interest are rarely explicitly recognized. In particular, as I discuss below, the notion of noncontingency of association is clos ...
... molecular biologists. Moreover, in the philosophical literature there are discussions of closely related ideas, although the connections with causal notions of biological interest are rarely explicitly recognized. In particular, as I discuss below, the notion of noncontingency of association is clos ...