Genome
... The goal of this exercise is to gain experience using BLAST, particularly blastN, and the UCSC genome browser to answer biologically relevant questions. Jian Ma | Sequence Comparison and Genome Alignment | 2015 ...
... The goal of this exercise is to gain experience using BLAST, particularly blastN, and the UCSC genome browser to answer biologically relevant questions. Jian Ma | Sequence Comparison and Genome Alignment | 2015 ...
structure and mechanism of dna polymerases
... idea, proposed by Watson and Crick (1953a,b) based on the structure of DNA, was that selection could be determined by the A‐T and G‐C hydrogen bond–mediated base pairing. However, replicative polymerases have error rates for nucleotide insertion in the range of 10–3–10–6/base replicated and the diff ...
... idea, proposed by Watson and Crick (1953a,b) based on the structure of DNA, was that selection could be determined by the A‐T and G‐C hydrogen bond–mediated base pairing. However, replicative polymerases have error rates for nucleotide insertion in the range of 10–3–10–6/base replicated and the diff ...
Characterization of sparfloxacin-resistant mutants of Staphylococcus aureus Original article
... New fluoroquinolones are broad-spectrum antibacterial agents that showed good activity against Gram-negative bacteria [1–4] and Staphylococcus aureus at the time of their introduction [5]. The continuous rise in fluoroquinolone resistance levels in both Gram-negative and Gram-positive microorganisms ...
... New fluoroquinolones are broad-spectrum antibacterial agents that showed good activity against Gram-negative bacteria [1–4] and Staphylococcus aureus at the time of their introduction [5]. The continuous rise in fluoroquinolone resistance levels in both Gram-negative and Gram-positive microorganisms ...
Developmental Validation of the Quantifiler Real-Time
... accumulates to the point where it can be detected over background fluorescence, and crosses a set fluorescence threshold. The cycle number at which the fluorescent signal crosses the threshold is defined as the “Threshold Cycle”, or CT . There is an exact inverse mathematical relationship between th ...
... accumulates to the point where it can be detected over background fluorescence, and crosses a set fluorescence threshold. The cycle number at which the fluorescent signal crosses the threshold is defined as the “Threshold Cycle”, or CT . There is an exact inverse mathematical relationship between th ...
The Preservation and Persistence of Human DNA in Soil during
... and Tibbett (2008) stress the need to understand basic issues surrounding taphonomy, with particular emphasis on changes in site ecology with the release of organic material into the soil during cadaver decomposition. It is with this focus in mind that the current ...
... and Tibbett (2008) stress the need to understand basic issues surrounding taphonomy, with particular emphasis on changes in site ecology with the release of organic material into the soil during cadaver decomposition. It is with this focus in mind that the current ...
Document
... The goal of this exercise is to gain experience using BLAST, particularly blastN, and the UCSC genome browser to answer biologically relevant questions. Jian Ma | Sequence Comparison and Genome Alignment ...
... The goal of this exercise is to gain experience using BLAST, particularly blastN, and the UCSC genome browser to answer biologically relevant questions. Jian Ma | Sequence Comparison and Genome Alignment ...
Paediatric HIV Lit Review - Factors Affecting ART Uptake
... Overall the proportion of Children under ART is low (only 8.3%) The Eastern region appears to have high numbers of children enrolled – probably because good record keeping. Low access in the Mid-Northern data set could be due to poor record keeping or real difficulties in acces Research center ...
... Overall the proportion of Children under ART is low (only 8.3%) The Eastern region appears to have high numbers of children enrolled – probably because good record keeping. Low access in the Mid-Northern data set could be due to poor record keeping or real difficulties in acces Research center ...
Analysis of Guanine Oxidation Products in Double
... guanine-rich sequences can form quadruplex structures. In a previous study using 6-mer DNA d(TGGGGT), which is the shortest oligomer capable of forming quadruplex structures, we demonstrated that guanine oxidation products of quadruplex DNA differ from those of single-stranded DNA. Therefore, the ho ...
... guanine-rich sequences can form quadruplex structures. In a previous study using 6-mer DNA d(TGGGGT), which is the shortest oligomer capable of forming quadruplex structures, we demonstrated that guanine oxidation products of quadruplex DNA differ from those of single-stranded DNA. Therefore, the ho ...
Evaluation of genomic DNA from paraffin
... Dogs that are clinically diagnosed with DCM reveal two distinct histological forms of DCM. Cardiomyopathy of Boxers and Doberman Pinschers are called “fatty infiltration-degenerative” type and in many giant, large- and medium-sized breeds DCM can be classified as “attenuated wavy fiber” type. Attenu ...
... Dogs that are clinically diagnosed with DCM reveal two distinct histological forms of DCM. Cardiomyopathy of Boxers and Doberman Pinschers are called “fatty infiltration-degenerative” type and in many giant, large- and medium-sized breeds DCM can be classified as “attenuated wavy fiber” type. Attenu ...
Obligate phototrophy in cyanobacteria: more than a lack of sugar
... acid sequence identity with, and a predictable structure similar to, that of a family of non-phosphorylating sugar carriers largely distributed among prokaryotes and eukaryotes. It thus appeared probable that the carrier of other facultative phototrophic cyanobacteria using glucose as substrate woul ...
... acid sequence identity with, and a predictable structure similar to, that of a family of non-phosphorylating sugar carriers largely distributed among prokaryotes and eukaryotes. It thus appeared probable that the carrier of other facultative phototrophic cyanobacteria using glucose as substrate woul ...
dissertationes biologicae universitatis tartuensis 118
... Rep from Escherichia coli have been solved for the helicases from SF1 (Subramanya et al., 1996; Korolev et al., 1997). In addition, the crystal structure of the RecBCD complex from E. coli has been solved (Singleton et al., 2004), two components of which — RecB and RecD — are SF1 helicases (Boehmer ...
... Rep from Escherichia coli have been solved for the helicases from SF1 (Subramanya et al., 1996; Korolev et al., 1997). In addition, the crystal structure of the RecBCD complex from E. coli has been solved (Singleton et al., 2004), two components of which — RecB and RecD — are SF1 helicases (Boehmer ...
Indirect Recognition in Sequence
... taken from the literature for 32 IHF sites that have been carefully characterized quantitatively suggested both the need and an approach to separate effects of DNA flexibility from sequence-specific, albeit still indirect, recognition mechanisms that pertain to the conserved elements. A subsequent a ...
... taken from the literature for 32 IHF sites that have been carefully characterized quantitatively suggested both the need and an approach to separate effects of DNA flexibility from sequence-specific, albeit still indirect, recognition mechanisms that pertain to the conserved elements. A subsequent a ...
Characterisation of marsupial PHLDA2 reveals eutherian specific acquisition of imprinting Open Access
... controls placental growth [35-37] and acts as a true rheostat for placental growth [36]. Recently, using a single copy transgenic mouse, Tunster et al., (2010) reported that Phlda2 regulates extraembryonic energy stores. Two-fold over-expression of Phlda2 caused a 60% loss of the spongiotrophoblast ...
... controls placental growth [35-37] and acts as a true rheostat for placental growth [36]. Recently, using a single copy transgenic mouse, Tunster et al., (2010) reported that Phlda2 regulates extraembryonic energy stores. Two-fold over-expression of Phlda2 caused a 60% loss of the spongiotrophoblast ...
DNA breathing dynamics distinguish binding from nonbinding
... Oncology, University of Texas Southwestern Medical School, Dallas, TX, 75390 USA ...
... Oncology, University of Texas Southwestern Medical School, Dallas, TX, 75390 USA ...
Applied and Environmental Microbiology
... mineralization. There is evidence that proteases from Bacillus cereus and B. mycoides, which belong to the neutral metalloprotease class, play an important role in proteolytic processes in soils (6, 16–18). The DNA sequence of the B. cereus thermolysin-like enzyme has a high degree of homology to th ...
... mineralization. There is evidence that proteases from Bacillus cereus and B. mycoides, which belong to the neutral metalloprotease class, play an important role in proteolytic processes in soils (6, 16–18). The DNA sequence of the B. cereus thermolysin-like enzyme has a high degree of homology to th ...
Etude Annotation
... My screen isn’t quite big enough to comfortably display both the frame window and my sequence window, so I will be flipping back and forth between the windows. In the Frames window, I see my fo ...
... My screen isn’t quite big enough to comfortably display both the frame window and my sequence window, so I will be flipping back and forth between the windows. In the Frames window, I see my fo ...
The universal reagent for genome tailoring
... The frequency of homologous recombination using a given target vector depends on many factors, including the amount of mismatch between the arms of homology and the targeted gene. Although the targeting frequency may negatively correlate with the number of mismatches, the targeting is still feasible ...
... The frequency of homologous recombination using a given target vector depends on many factors, including the amount of mismatch between the arms of homology and the targeted gene. Although the targeting frequency may negatively correlate with the number of mismatches, the targeting is still feasible ...
Mechanistic Comparison of High-Fidelity and Error
... Discovered in the early 1970s, DNA polymerase β (Pol β) was the first mammalian DNA repair polymerase to be characterized. Its relatively small size (39 kD) and the fact that it lacks an exonuclease proofreading activity make it a tractable system for studying the mechanism by which fidelity is achi ...
... Discovered in the early 1970s, DNA polymerase β (Pol β) was the first mammalian DNA repair polymerase to be characterized. Its relatively small size (39 kD) and the fact that it lacks an exonuclease proofreading activity make it a tractable system for studying the mechanism by which fidelity is achi ...
Sequence requirements for function of the
... gene loci (Asano and Wharton, 1999; Calvi et al., 1998; Royzman et al., 1999). The data demonstrate that Drosophila chorion gene amplification uses evolutionarily conserved machinery for initiation; however, some mechanism must exist to uniquely mark the chorion gene loci origins for activation duri ...
... gene loci (Asano and Wharton, 1999; Calvi et al., 1998; Royzman et al., 1999). The data demonstrate that Drosophila chorion gene amplification uses evolutionarily conserved machinery for initiation; however, some mechanism must exist to uniquely mark the chorion gene loci origins for activation duri ...
HIV-1 resistance analyses from therapy-naïve
... events of SIV strains into the human host still frequently occur and it remains unclear why HIV in its current form has become so predominant (Kalish et al., 2005, Weiss and Wrangham, 1999). ...
... events of SIV strains into the human host still frequently occur and it remains unclear why HIV in its current form has become so predominant (Kalish et al., 2005, Weiss and Wrangham, 1999). ...
Neandertal DNA Sequences and the Origin of Modern Humans
... In addition, three sequence positions carried changes occurring in five and four clones. These changes were not observed in combination in the previous four amplifications covering this sequence segment. Since they occurred in only one amplification product, they are probably due to polymerase error ...
... In addition, three sequence positions carried changes occurring in five and four clones. These changes were not observed in combination in the previous four amplifications covering this sequence segment. Since they occurred in only one amplification product, they are probably due to polymerase error ...
Replication of Nuclear DNA
... All eukaryotic organisms organise their DNA for replication as multiple units known as replicons. In cells undergoing DNA replication, these multiple units are readily visualised by fibre autoradiography (Cairns 1966; Huberman and Riggs 1968). This was first applied to plants by Van’t Hof (1975) and ...
... All eukaryotic organisms organise their DNA for replication as multiple units known as replicons. In cells undergoing DNA replication, these multiple units are readily visualised by fibre autoradiography (Cairns 1966; Huberman and Riggs 1968). This was first applied to plants by Van’t Hof (1975) and ...
The DnaE polymerase from Deinococcus radiodurans features
... competence, D. radiodurans does also feature additional and peculiar biochemical properties responsible for genome integrity maintenance. In particular, when considering that ionizing radiations induce severe oxidative stress, it was realized that the radiation-resistance of D. radiodurans is mainly ...
... competence, D. radiodurans does also feature additional and peculiar biochemical properties responsible for genome integrity maintenance. In particular, when considering that ionizing radiations induce severe oxidative stress, it was realized that the radiation-resistance of D. radiodurans is mainly ...
A structural determinant in the uracil DNA glycosylase superfamily
... (1–7). Family 1 UNG is a highly efficient enzyme that ex* To ...
... (1–7). Family 1 UNG is a highly efficient enzyme that ex* To ...