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... manipulation of paramagnetic beads using a gradient of magnetic field [14]. The two techniques have been developing head to head to achieve nowadays a remarkable level of sophistication. They have their own unique advantages but, largely, offer similar capabilities and require the same workflow. The ...
... manipulation of paramagnetic beads using a gradient of magnetic field [14]. The two techniques have been developing head to head to achieve nowadays a remarkable level of sophistication. They have their own unique advantages but, largely, offer similar capabilities and require the same workflow. The ...
CORPORATE RESPONSIBILITY AT DNA CORPORATE
... a Finnish education development company. The initiative will also build a database for education innovations, which will be made available to schools and teachers globally. Other plans include a book, documentary and a series of international seminars. In November 2016, DNA organised a workshop for ...
... a Finnish education development company. The initiative will also build a database for education innovations, which will be made available to schools and teachers globally. Other plans include a book, documentary and a series of international seminars. In November 2016, DNA organised a workshop for ...
Mycobacterium tuberculosis DNA gyrase ATPase domain structures
... nanomachines present in all organisms. They solve topological problems of DNA by temporarily introducing a double-stranded break into one DNA segment and transporting another DNA [TDNA (transported DNA)] duplex through this temporary break [1,2]. Most bacteria have two type II topoisomerases (DNA gy ...
... nanomachines present in all organisms. They solve topological problems of DNA by temporarily introducing a double-stranded break into one DNA segment and transporting another DNA [TDNA (transported DNA)] duplex through this temporary break [1,2]. Most bacteria have two type II topoisomerases (DNA gy ...
Application Note: Targeted sequencing and chromosomal haplotype
... complete loci on the basis of crosslinking physically proximal sequences. Unlike other targeted sequencing methods, TLA works without prior detailed locus information, as one primer pair is sufficient to amplify tens to hundreds of kilobases of DNA surrounding that locus. In a separate application o ...
... complete loci on the basis of crosslinking physically proximal sequences. Unlike other targeted sequencing methods, TLA works without prior detailed locus information, as one primer pair is sufficient to amplify tens to hundreds of kilobases of DNA surrounding that locus. In a separate application o ...
Alu - Environmental
... Alu elements • Alu elements are only found in the primate branch • Each Alu insertion is a unique event and is inherited from each parent • Most occurred millions of years ago and are often on both pairs of chromosomes • There are Alu elements that have occurred since humans branched from other pri ...
... Alu elements • Alu elements are only found in the primate branch • Each Alu insertion is a unique event and is inherited from each parent • Most occurred millions of years ago and are often on both pairs of chromosomes • There are Alu elements that have occurred since humans branched from other pri ...
Full text - PAHdb - McGill University
... interspersed repeats to be 42.2% in the PAH gene, a value typical for a mammalian gene. RepeatMasker data and a table of DNA variations, mostly SNPs, are shown in PAHdb. Repetitive DNA is often the cause of large genomic deletions and duplications [Antonarakis et al., 2001]. A search for Alu repeat ...
... interspersed repeats to be 42.2% in the PAH gene, a value typical for a mammalian gene. RepeatMasker data and a table of DNA variations, mostly SNPs, are shown in PAHdb. Repetitive DNA is often the cause of large genomic deletions and duplications [Antonarakis et al., 2001]. A search for Alu repeat ...
Poly ADP-ribosylation: a histone shuttle mechanism in DNA excision
... ADP-ribose pyrophosphorylase (Tanuma, 1989). Further investigations are needed to determine the significance of these other activities in poly(ADPribose) metabolism. Poly(ADP-ribose) Polymerase ...
... ADP-ribose pyrophosphorylase (Tanuma, 1989). Further investigations are needed to determine the significance of these other activities in poly(ADPribose) metabolism. Poly(ADP-ribose) Polymerase ...
Principles and challenges of genome
... As noted above, 5meC is not readily distinguished from unmethylated cytosine by hybridization-based methods and, as DNA methyltransferases are not present during PCR or in biological cloning systems, DNA methylation information is erased during amplification. some investigators have suggested that i ...
... As noted above, 5meC is not readily distinguished from unmethylated cytosine by hybridization-based methods and, as DNA methyltransferases are not present during PCR or in biological cloning systems, DNA methylation information is erased during amplification. some investigators have suggested that i ...
Use of novel assays to measure in vivo base excision DNA repair
... The BER system consists of a group of lesion-specific enzymes termed DNA glycosylases, which recognize and excise specific base modifications, resulting in AP sites. Other enzymes act in a series of steps to fill in the missing base, and ultimately restore the correct sequence (Krokan, Nilsen, Skor ...
... The BER system consists of a group of lesion-specific enzymes termed DNA glycosylases, which recognize and excise specific base modifications, resulting in AP sites. Other enzymes act in a series of steps to fill in the missing base, and ultimately restore the correct sequence (Krokan, Nilsen, Skor ...
Biology 30 June 2000 Grade 12 Diploma Examinations
... regeneration of damaged axons, peripheral nerve axons can regenerate. ...
... regeneration of damaged axons, peripheral nerve axons can regenerate. ...
MA112 Expresso® Rhamnose SUMO Cloning and
... is designed for expression in E. coli, fusion clones constructed using this system can also be transferred into eukaryotic expression vectors for expression of SUMO-tagged proteins in mammalian or insect cells. The rhaP BAD promoter is a versatile tool for protein expression. In the absence of rhamn ...
... is designed for expression in E. coli, fusion clones constructed using this system can also be transferred into eukaryotic expression vectors for expression of SUMO-tagged proteins in mammalian or insect cells. The rhaP BAD promoter is a versatile tool for protein expression. In the absence of rhamn ...
Identification of editing positions in the ndhB transcript from maize
... of the used sequencing primers). The positions of the six editing sites within the sequence of the ndhB coding strand and with respect to the encoded amino acids are indicated in Figure 2. Five of the affected ndhB triplets are edited at the second codon position as was observed for all chloroplast ...
... of the used sequencing primers). The positions of the six editing sites within the sequence of the ndhB coding strand and with respect to the encoded amino acids are indicated in Figure 2. Five of the affected ndhB triplets are edited at the second codon position as was observed for all chloroplast ...
The National DNA Database
... Except for identical siblings, each person’s DNA is unique. The technology used to obtain DNA profiles for The National DNA Database® does not examine every single difference between people’s DNA. It is restricted to looking at only specific areas of the DNA, known as short tandem repeats (STR’s). S ...
... Except for identical siblings, each person’s DNA is unique. The technology used to obtain DNA profiles for The National DNA Database® does not examine every single difference between people’s DNA. It is restricted to looking at only specific areas of the DNA, known as short tandem repeats (STR’s). S ...
Solving a Crime Using DNA Analysis and Chemistry
... The manager of the college food services promised the president of the college a plate of his favorite chocolate chip cookies for the upcoming meeting with the executive council of the Board of Directors. The chocolate chip cookies are a specialty of Chef Lombardo's and are famous across the college ...
... The manager of the college food services promised the president of the college a plate of his favorite chocolate chip cookies for the upcoming meeting with the executive council of the Board of Directors. The chocolate chip cookies are a specialty of Chef Lombardo's and are famous across the college ...
Unraveling DNA helicases
... ssDNA translocases, whereas SF2 members (RecG, PriA and HSV NS3) are dsDNA translocases. This property may be the distinction between the SF1 and SF2 family. Another group called family 4 (F4) contains five motifs (1, 1a, 2–4) [3]. The seven motifs of SF1 and SF2 are usually clustered in a region of ...
... ssDNA translocases, whereas SF2 members (RecG, PriA and HSV NS3) are dsDNA translocases. This property may be the distinction between the SF1 and SF2 family. Another group called family 4 (F4) contains five motifs (1, 1a, 2–4) [3]. The seven motifs of SF1 and SF2 are usually clustered in a region of ...
Discovering the Distribution of Palindromic Sequences in the
... mutations on the SMAD4 database were converted into a readable .csv file (6 columns: (1) Segment (exon, etc.) (2) Type of mutation (3) Start location of the mutation (integer) (4) End location of the mutation (integer) (5) Nucleotide change (6) Type of protein change) titled “mutations.csv”. Three J ...
... mutations on the SMAD4 database were converted into a readable .csv file (6 columns: (1) Segment (exon, etc.) (2) Type of mutation (3) Start location of the mutation (integer) (4) End location of the mutation (integer) (5) Nucleotide change (6) Type of protein change) titled “mutations.csv”. Three J ...
Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
... required for the formationof an inverted dimer plasmid, we investigated the DNA concentration dependence of transformation with linearized plasmid DNA. If the formation of an inverted dimer plasmid requires only a single plasmid molecule, this dependence should be first order. A requirement for two ...
... required for the formationof an inverted dimer plasmid, we investigated the DNA concentration dependence of transformation with linearized plasmid DNA. If the formation of an inverted dimer plasmid requires only a single plasmid molecule, this dependence should be first order. A requirement for two ...
Statistical analysis of simple repeats in the human genome
... associated with any of the single- or di-nucleotide probabilities, nor with any mnucleotide probability, with m42: This finding is consistent with the general acceptance of the failure of zeroth- and first-order Markovian models of simple repeats to fully account for ‘‘linguistic’’ features of non-cod ...
... associated with any of the single- or di-nucleotide probabilities, nor with any mnucleotide probability, with m42: This finding is consistent with the general acceptance of the failure of zeroth- and first-order Markovian models of simple repeats to fully account for ‘‘linguistic’’ features of non-cod ...
Illustrating Python via Bioinformatics Examples
... make the difference between the cells. This regulation of genes is orchestrated by an immensely complex mechanism, which we have only started to understand. A central part of this mechanism consists of molecules called transcription factors that float around in the cell and attach to DNA, and in doi ...
... make the difference between the cells. This regulation of genes is orchestrated by an immensely complex mechanism, which we have only started to understand. A central part of this mechanism consists of molecules called transcription factors that float around in the cell and attach to DNA, and in doi ...
Distortion of quantitative genomic and expression
... genomic DNA, in the presence and absence of Cot-1 DNA. Reactions were hybridized, washed, stained with SPE and then analyzed by flow cytometry. Quantitative PCR QPCR and data analysis were performed using the Chromo4 QPCR system (BioRad Laboratories, Hercules, CA). Primers and amplified intervals we ...
... genomic DNA, in the presence and absence of Cot-1 DNA. Reactions were hybridized, washed, stained with SPE and then analyzed by flow cytometry. Quantitative PCR QPCR and data analysis were performed using the Chromo4 QPCR system (BioRad Laboratories, Hercules, CA). Primers and amplified intervals we ...
as a PDF - CiteSeerX
... chromosomes. In mitotically growing cells of lower organisms, recombination provides for efficient repair of DNA damage, particularly double-strand breaks, through interactions between homologs or sister chromatids. In mammals, somatic recombination is an important component in the development of th ...
... chromosomes. In mitotically growing cells of lower organisms, recombination provides for efficient repair of DNA damage, particularly double-strand breaks, through interactions between homologs or sister chromatids. In mammals, somatic recombination is an important component in the development of th ...
Human Inheritance Lab
... Tongue roller (T): the ability to roll their tongues into a “U” shape when it is extended. o Roller- heterozygous o People with the recessive alleles cannot roll their tongues. Tongue folder (F): the ability to fold their tongues over while sticking it out. o Folder- homozygous dominant o People wit ...
... Tongue roller (T): the ability to roll their tongues into a “U” shape when it is extended. o Roller- heterozygous o People with the recessive alleles cannot roll their tongues. Tongue folder (F): the ability to fold their tongues over while sticking it out. o Folder- homozygous dominant o People wit ...
Nucleotide sequence and taxonomic value of the
... peptidoglycan (Bavoil et al., 1984). Disulphide interlinking between MOMP polypeptide chains is thought to play an important role in regulating MOMP porin function, nutrient ingress through the EB outer envelope and ultimately the initiation of chlamydial differentiation itself (Bavoil et al., 1984) ...
... peptidoglycan (Bavoil et al., 1984). Disulphide interlinking between MOMP polypeptide chains is thought to play an important role in regulating MOMP porin function, nutrient ingress through the EB outer envelope and ultimately the initiation of chlamydial differentiation itself (Bavoil et al., 1984) ...
2- pcr primer design and reaction optimisation
... Polymerase chain reaction (PCR) is still the most popular amplification method, however alternatives to PCR have successfully invaded the area. The emergence of such methodologies has significantly widened the range of approaches for DNA amplification and dramatically improved the technological abil ...
... Polymerase chain reaction (PCR) is still the most popular amplification method, however alternatives to PCR have successfully invaded the area. The emergence of such methodologies has significantly widened the range of approaches for DNA amplification and dramatically improved the technological abil ...
Reference genome sequence of the model plant Setaria
... element classes, insertion dates were approximated for each element family (Supplementary Fig. 3). The data indicate a very recent burst of LTR retrotransposon amplification, peaking within the last few hundred thousand years, whereas the LINE and CACTA elements exhibit a broader range of activity o ...
... element classes, insertion dates were approximated for each element family (Supplementary Fig. 3). The data indicate a very recent burst of LTR retrotransposon amplification, peaking within the last few hundred thousand years, whereas the LINE and CACTA elements exhibit a broader range of activity o ...