File
... the ability to isolate a specific segment of DNA, modify it in a test tube, and transfer it back into the same or a different cell to create a genetically modified organism represents perhaps the most significant advance in the history of biology, with profound consequences for the future of our, an ...
... the ability to isolate a specific segment of DNA, modify it in a test tube, and transfer it back into the same or a different cell to create a genetically modified organism represents perhaps the most significant advance in the history of biology, with profound consequences for the future of our, an ...
Redesign of the monomer–monomer interface of Cre recombinase
... The loxP site consists of two 13 bp palindromic half-sites separated by an asymmetric 8 bp spacer that gives loxP its direction. The utility of targeting Cre mutants to altered RT sites is severely compromised if only pseudo-palindromic sites may be considered. This limitation has been addressed by ...
... The loxP site consists of two 13 bp palindromic half-sites separated by an asymmetric 8 bp spacer that gives loxP its direction. The utility of targeting Cre mutants to altered RT sites is severely compromised if only pseudo-palindromic sites may be considered. This limitation has been addressed by ...
Gene Section EVI1 (ecotropic viral integration site 1 (EVI1) and
... absent limb buds, a pale yolk sac and placenta, abnormal development of the nervous system and the heart, and massive haemorrhaging. EVI1 is thought to exert its biological functions mainly by acting as a transcription factor. In addition, however, EVI1 has been reported to inhibit c-jun Nterminal k ...
... absent limb buds, a pale yolk sac and placenta, abnormal development of the nervous system and the heart, and massive haemorrhaging. EVI1 is thought to exert its biological functions mainly by acting as a transcription factor. In addition, however, EVI1 has been reported to inhibit c-jun Nterminal k ...
Properties of Mitotic and Meiotic Recombination in the
... array, and heterozygous for markers flanking the array, we measured inter-homolog recombination and intra-/sister-chromatid exchange in the CUP1 locus. The rate of intra-/sister chromatid recombination exceeded the rate of inter-homolog recombination by more than ten-fold. Loss of the Rad51 and Rad5 ...
... array, and heterozygous for markers flanking the array, we measured inter-homolog recombination and intra-/sister-chromatid exchange in the CUP1 locus. The rate of intra-/sister chromatid recombination exceeded the rate of inter-homolog recombination by more than ten-fold. Loss of the Rad51 and Rad5 ...
Document
... DNA controls all the chemical changes which take place in cells The kind of cell which is formed, (muscle, blood, nerve etc) is controlled by DNA The kind of organism which is produced (buttercup, giraffe, herring, human etc) is controlled by DNA ...
... DNA controls all the chemical changes which take place in cells The kind of cell which is formed, (muscle, blood, nerve etc) is controlled by DNA The kind of organism which is produced (buttercup, giraffe, herring, human etc) is controlled by DNA ...
ARTICLES - Weizmann Institute of Science
... Eukaryotic genomes are packaged into nucleosome particles that occlude the DNA from interacting with most DNA binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the nucleosome structure. However, it ...
... Eukaryotic genomes are packaged into nucleosome particles that occlude the DNA from interacting with most DNA binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the nucleosome structure. However, it ...
Evolution of Closely Linked Gene Pairs in
... between human h2h pairs (Takai and Jones 2004). It is curious, however, that enrichment in closely linked h2h pairs is reported to be limited to mammals (Koyanagi et al. 2005); one would expect them to accumulate in all evolutionary lineages. We have therefore examined the evolution of closely linke ...
... between human h2h pairs (Takai and Jones 2004). It is curious, however, that enrichment in closely linked h2h pairs is reported to be limited to mammals (Koyanagi et al. 2005); one would expect them to accumulate in all evolutionary lineages. We have therefore examined the evolution of closely linke ...
Inference of homologous recombination in bacteria using whole
... its clonal genealogy [7] irrespective of how frequently recombination happened, by tracing back in time the ancestry of the isolates following the line of ancestry of the recipient (and not that of the donor) whenever recombination took place. 1 The clonal genealogy is a bifurcating tree where each ...
... its clonal genealogy [7] irrespective of how frequently recombination happened, by tracing back in time the ancestry of the isolates following the line of ancestry of the recipient (and not that of the donor) whenever recombination took place. 1 The clonal genealogy is a bifurcating tree where each ...
Document
... • Adenine must pair with Thymine • Guanine must pair with Cytosine • Their amounts in a given DNA molecule will be about the same. ...
... • Adenine must pair with Thymine • Guanine must pair with Cytosine • Their amounts in a given DNA molecule will be about the same. ...
The Molecular Basis of Imidazolinone Herbicide Resistance in
... been partially purified and characterized (Muhitch et al., 1987). Since ALS is a labile enzyme it is very difficult to purify the active protein to homogeneity for further characterization and preparation of antibodies. Genetic analysis indicated that ALS genes are located at a single locus in the n ...
... been partially purified and characterized (Muhitch et al., 1987). Since ALS is a labile enzyme it is very difficult to purify the active protein to homogeneity for further characterization and preparation of antibodies. Genetic analysis indicated that ALS genes are located at a single locus in the n ...
document
... Precise sequence information was needed to design a gene specific TaQman assay for bm3. Public information was used to design 2 oligos which amplified the partial COMT gene from the bm3 and non bm3 lines, and then these fragments were then cloned and sent to Cogenics to obtain the full length sequen ...
... Precise sequence information was needed to design a gene specific TaQman assay for bm3. Public information was used to design 2 oligos which amplified the partial COMT gene from the bm3 and non bm3 lines, and then these fragments were then cloned and sent to Cogenics to obtain the full length sequen ...
Investigating the DNA-Binding Interactions of Small
... Figure 2.11. (A) Simplied directions to use BioPORTER peptide delivery kit. (B) Summary of how the BioPORTER reagent works. ...
... Figure 2.11. (A) Simplied directions to use BioPORTER peptide delivery kit. (B) Summary of how the BioPORTER reagent works. ...
genomebiology.com
... revealed that TEs are less likely to be fixed within transcribed regions relative to orthologous regions in human and mouse [16]. In G. gallus, D. rerio and C. intestinalis, 33.2%, 47.3% and 39.4% of TEs reside within introns, respectively, whereas in the human genome, approximately 60% of TEs resid ...
... revealed that TEs are less likely to be fixed within transcribed regions relative to orthologous regions in human and mouse [16]. In G. gallus, D. rerio and C. intestinalis, 33.2%, 47.3% and 39.4% of TEs reside within introns, respectively, whereas in the human genome, approximately 60% of TEs resid ...
Chapter 22: SV40 and Polyomavirus DNA Replication
... redundant sets of sequences in both the SV40 and PyV regulatory regions that can act as auxiliary elements (Fig. 1). For example, either one of two regions, termed alpha or beta, functions as an auxiliary element for PyV DNA replication (Muller et al. 1988). Similarly, the 21-bp or the 72-bp repeat ...
... redundant sets of sequences in both the SV40 and PyV regulatory regions that can act as auxiliary elements (Fig. 1). For example, either one of two regions, termed alpha or beta, functions as an auxiliary element for PyV DNA replication (Muller et al. 1988). Similarly, the 21-bp or the 72-bp repeat ...
Genome organization in dicots: Genome duplication in Arabidopsis
... Comparative genome analyses between soybean and Arabidopsis could facilitate cross-utilization of genetic resources and tools of both species and could shed light on evolutionary events associated with the divergence of their seemingly disparate genomes. The public availability of data generated fro ...
... Comparative genome analyses between soybean and Arabidopsis could facilitate cross-utilization of genetic resources and tools of both species and could shed light on evolutionary events associated with the divergence of their seemingly disparate genomes. The public availability of data generated fro ...
Phylogenetic Relationships between the Western Aster Yellows
... dodecyl sulfate, and the DNA was bound to the filters by alkaline hydrolysis and baking. Nitrocellulose filters containing recombinant phage generated from the EcoRI fragment transformation were hybridized with the 5’ 18-mer. After clones that hybridized with the 18-mer were identified, the bound pr ...
... dodecyl sulfate, and the DNA was bound to the filters by alkaline hydrolysis and baking. Nitrocellulose filters containing recombinant phage generated from the EcoRI fragment transformation were hybridized with the 5’ 18-mer. After clones that hybridized with the 18-mer were identified, the bound pr ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... and differences among sequences so that properties of DNA sequences can be understood (Matsumoto et al., 2000; Li et al., 2001). Current compression algorithms work by finding redundant information within the DNA sequence. For example, most compression algorithms tried to exploit exact/approximate r ...
... and differences among sequences so that properties of DNA sequences can be understood (Matsumoto et al., 2000; Li et al., 2001). Current compression algorithms work by finding redundant information within the DNA sequence. For example, most compression algorithms tried to exploit exact/approximate r ...
Directionality in FLP Protein-promoted Site
... recombination occurs. The 13-base pair repeats are shaded. Cleav- presence of ethidium bromide. The sequence of all synthetic inserts age sites areindicated with arrows. See test for details. was verified by recloning the fragments into the bacterophage M13 derivative, mp8 and sequencing (12). For i ...
... recombination occurs. The 13-base pair repeats are shaded. Cleav- presence of ethidium bromide. The sequence of all synthetic inserts age sites areindicated with arrows. See test for details. was verified by recloning the fragments into the bacterophage M13 derivative, mp8 and sequencing (12). For i ...
Introduction - Stellenbosch University
... Distance from the sun to Uranus = 2.8 ×109km Each single human contains enough DNA to stretch from the sun to Uranus Bioinformatics, Stellenbosch University ...
... Distance from the sun to Uranus = 2.8 ×109km Each single human contains enough DNA to stretch from the sun to Uranus Bioinformatics, Stellenbosch University ...
the significance of hydrogen bonding
... ribozymes have been an ambitious goal for many research groups. Modelling the active site of an enzyme by smaller molecules may provide more detailed information about the mechanism of action. Such compounds could also have various applications in the fields of medicinal chemistry and molecular biol ...
... ribozymes have been an ambitious goal for many research groups. Modelling the active site of an enzyme by smaller molecules may provide more detailed information about the mechanism of action. Such compounds could also have various applications in the fields of medicinal chemistry and molecular biol ...
DNA PPT - Alevelsolutions
... molecule. The double helix enzyme known as DNA helicase untwists the DNA and breaks the Hbonds between the bases. • Nucleotides then pair up with complimentary bases – DNA Polymerase link these bases together • Each of the new DNA molecule is made up of one old strand and one new strand – hence the ...
... molecule. The double helix enzyme known as DNA helicase untwists the DNA and breaks the Hbonds between the bases. • Nucleotides then pair up with complimentary bases – DNA Polymerase link these bases together • Each of the new DNA molecule is made up of one old strand and one new strand – hence the ...
Fragaria multicipita - DigitalCommons@University of Nebraska
... primed by primer pair P1/P7 but from only rrnA in PCR primed by primer pair R16mF2/R16mR1. Preferential amplification of DNA from operon rrnA was explained by base mismatches between the R16mF2/R16mR1 primers and primer annealing sites in rrnB. The results revealed potential for classification of a ...
... primed by primer pair P1/P7 but from only rrnA in PCR primed by primer pair R16mF2/R16mR1. Preferential amplification of DNA from operon rrnA was explained by base mismatches between the R16mF2/R16mR1 primers and primer annealing sites in rrnB. The results revealed potential for classification of a ...
UK guideline for the use of HIV Post-Exposure Prophylaxis
... sexual health. The guideline is intended to be complementary to existing Department of Health and Expert Advisory Group on AIDS (EAGA) guidance on PEP.1 It is aimed primarily at clinicians and policy-makers in sexual health, sexual assault referral centres (SARCs), and primary and emergency care pro ...
... sexual health. The guideline is intended to be complementary to existing Department of Health and Expert Advisory Group on AIDS (EAGA) guidance on PEP.1 It is aimed primarily at clinicians and policy-makers in sexual health, sexual assault referral centres (SARCs), and primary and emergency care pro ...
A Novel CpG Island Set Identifies Tissue-Specific
... resulting library was sequenced to define a novel human blood CGI set that includes many that are not detected by current algorithms. Approximately half of CGIs were associated with annotated gene transcription start sites, the remainder being intra- or intergenic. Using an array representing over 1 ...
... resulting library was sequenced to define a novel human blood CGI set that includes many that are not detected by current algorithms. Approximately half of CGIs were associated with annotated gene transcription start sites, the remainder being intra- or intergenic. Using an array representing over 1 ...
New peptide and gene coding for same
... 151 amino acids wherein a sequence of from 26 to 151 corresponds to the sequence of the 7-hANP set forth in Figure 1; Figure 3 represents a base sequence coding for the amino acid sequence set forth in Figure 1; Figure 4 represents a base sequence coding for the amino acid sequence set forth in Figu ...
... 151 amino acids wherein a sequence of from 26 to 151 corresponds to the sequence of the 7-hANP set forth in Figure 1; Figure 3 represents a base sequence coding for the amino acid sequence set forth in Figure 1; Figure 4 represents a base sequence coding for the amino acid sequence set forth in Figu ...