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A molecular method for assessing meiofauna diversity in marine
A molecular method for assessing meiofauna diversity in marine

... found that using meiofauna data along with macrofauna data provides greater insight into the processes affecting a polluted area, since meiofauna are not as affected by physical disturbance as macrofauna. The cost of processing and identifying macrofauna is high and incorporating another component o ...
The dystrophin / utrophin homologues in Drosophila and in sea urchin
The dystrophin / utrophin homologues in Drosophila and in sea urchin

... consists of only the cysteine-rich and C-terminal domains of dystrophin (Bar et al., 1990; Lederfein et al., 1992). It is the most abundant non-muscle product of the DMD gene. The highest levels of Dp71 are found in the brain (Rapaport et al., 1992; Greenberg et al., 1996). The other known small pro ...
A set reduction and pattern matching problem motivated by Allele
A set reduction and pattern matching problem motivated by Allele

... 3. Design a primer pair to amplify the desired allele from the pool of sequences produced using the locus specific primer (Fig. 3). The three steps seem quite similar due to their common purpose: reduction of the existing set. However, they are distinct as the primers must be designed to leverage di ...
pDsRed-Monomer Vector Information
pDsRed-Monomer Vector Information

... Amino acid substitutions (DsRed→DsRed-Monomer) GCC→GAC (Ala-2 to Asp) mutation: 292–294 TCC→AAC (Ser-3 to Asn) mutation: 295–297 TCC→ACC (Ser-4 to Thr) mutation: 298–300 AAG→GAG (Lys-5 to Glu) mutation: 301–303 AAC→GAC (Asn-6 to Asp) mutation: 304–306 CGC→CAG (Arg-13 to Gln) mut ...
Nutrition and HIV Medications - I-Tech
Nutrition and HIV Medications - I-Tech

... What are common foods eaten? Any times when food is not available? Any recent weight loss? How much? Any current medications (consider modern and traditional medicines)? • What are specific food-medication interactions or common side effects of proposed ART regimen? Session 3: Nutrition and HIV Medi ...
Fidelity of Primate Cell Repair of a Double
Fidelity of Primate Cell Repair of a Double

... Dramatic differences in CTG length variations have been observed in several regions of the brain in DM1, HD, and DRPLA patients when compared with blood of the same patient (1). While many regions of the cerebral cortex show heterogeneous expansions larger than those observed in blood of the same pa ...
Identification and characterization of CHO endogenous gene
Identification and characterization of CHO endogenous gene

... affect correct posttranslational processing and may finally induce the premature activation of apoptosis. In addition, some viral promoters are cell cycle dependent and can also be silenced in certain stable cell lines resulting in a considerable heterogeneity within the cell population. These undes ...
Agarose Gel Electrophoresis
Agarose Gel Electrophoresis

... Copyright © 1991 by Current Protocols ...
Single nucleotide polymorphisms in the ectomycorrhizal mushroom
Single nucleotide polymorphisms in the ectomycorrhizal mushroom

... polymorphisms (SNPs) are among the fastest-developing categories in biomedical and biological research. This is mainly because SNPs are the most frequently observed differences between DNA sequences obtained from different individuals, or between alleles from within an individual in diploid or highe ...
DNA Review Sheet Plus 10 points on the exam tomorrow
DNA Review Sheet Plus 10 points on the exam tomorrow

... proteins 23. On what strand is the codon found (DNA, mRNA or tRNA)? mRNA 24. On what strand is the anticodon found (DNA, mRNA or tRNA)? ...
Crystal  Structure  of  Paired  Domain  -...
Crystal Structure of Paired Domain -...

... activity of paired domain was first demonstrated between the Drosophila paired protein (Prd) and the e5 DNA sequence in the evenskipped promoter (Treisman ...
NUCLEOTIDES AND NUCLEIC ACIDS
NUCLEOTIDES AND NUCLEIC ACIDS

... quence in the cell’s DNA. A segment of a DNA molecule that contains the information required for the synthesis of a functional biological product, whether protein or RNA, is referred to as a gene. A cell typically has many thousands of genes, and DNA molecules, not surprisingly, tend to be very larg ...
DNA Science A Hands-On Workshop - nslc.wustl.edu
DNA Science A Hands-On Workshop - nslc.wustl.edu

... segment of a DNA strand. PCR allows researchers to study very small amounts of DNA without resorting to laborious cloning procedures. The technique has had an impact in many areas of biology and has greatly facilitated the field of forensics. Although the DNA from different individuals is more alike ...
USB® Thermo Sequenase Cycle Sequencing Kit
USB® Thermo Sequenase Cycle Sequencing Kit

... convenience, RapidGel™ gel mixes are strongly recommended. RapidGel-XL formulations yield up to 40% more readable sequence per gel. See ‘Related products’ section for range of USB Ultrapure gel products. Specialized sequencing primers—Some sequencing projects will require the use of primers which ar ...
Aus dem  Max-Planck-Institut für Psychiatrie
Aus dem Max-Planck-Institut für Psychiatrie

... 1.1.1 Overview of Function All organisms strive towards maintaining their homeostasis, i.e. the dynamic equilibrium of their internal milieus that is essential for survival. The challenge of homeostasis by internal or external factors is classically referred to as “stress”. “Stress reaction” is the ...
Mechanisms of Unidirectional Translocation & Unwinding
Mechanisms of Unidirectional Translocation & Unwinding

... • A critical arginine residue (R522 in T7 gp4 helicase) from a neighboring subunit is within hydrogen-bonding distance of the gamma phosphate of NTP bound at the interface, and is implicated in transducing conformational changes between subunits of the hexamer. • The central channel of the ring is l ...
Rolling circle transcription on smallest size double stranded DNA
Rolling circle transcription on smallest size double stranded DNA

... While DNA is primarily known as the information carrier of living organisms it has found recent application as a synthetic nano-scale engineering material. DNA has several key characteristics that makes the molecule highly suited for the creation of designed structures such as high stiffness, ease o ...
HIV - Delaware General Health District
HIV - Delaware General Health District

... p24 antigen testing to exclude infection in children <18 months of age is not recommended because of poor sensitivity, especially in the presence of HIV antibody. Commercial tests for RNA and DNA detection have become widely available. Quantitative RNA tests have been approved by the Food and Drug A ...
MOLECULAR STRUCTURE OF DNA AND RNA
MOLECULAR STRUCTURE OF DNA AND RNA

... viral genetic material, the bacterial cytoplasm provides all of the machinery necessary to make viral proteins and DNA. The viral proteins and DNA assemble to make new viruses that are subsequently released from the cell by lysis (i.e., cell breakage). To verify that DNA is the genetic material of T ...
Chromosomal breakpoint positions suggest a direct role for radiation
Chromosomal breakpoint positions suggest a direct role for radiation

... chromosomal regions with increased lability (fragile) sites (Ehrlich et al., 1993; Stary and Sarasin, 1992; Hyrien et al., 1987). By contrast, our results indicate that in post-Chernobyl tumors the RET/PTC breakpoints were distributed relatively randomly across the respective introns, except for clu ...
Pharmaceutical HIV prevention technologies in the UK: six domains
Pharmaceutical HIV prevention technologies in the UK: six domains

... sexual HIV risk involves individuals negotiating protean ‘landscapes of risk’ (Green & Sobo, 2000; Lupton, 1999). Thus reductive or deficit models of risk have been supplanted by an approach that characterises risk as productive in terms of the self and the possibilities afforded by intimate relatio ...
The Structures of DNA and RNA
The Structures of DNA and RNA

... the hereditary information within chromosomes, immediately focused attention on its structure. It was hoped that knowledge of the structure would reveal how DNA carries the genetic messages that are replicated when chromosomes divide to produce two identical copies of themselves. During the late 194 ...
Chpt8_RecombineDNA.doc
Chpt8_RecombineDNA.doc

... from two parental DNA molecules or different segments of the same DNA molecule; this will be the topic of this chapter. Transposition is a highly specialized form of recombination in which a segment of DNA moves from one location to another, either on the same chromosome or a different chromosome; t ...
UCSC genome support forum
UCSC genome support forum

... Identifying rRNA in the Genome Browser is going to depend on which assembly you are using as some assemblies have better annotation than others. If you are looking at the human (hg19, hg38) or mouse (mm10) genomes, you can use the "GENCODE Gene Annotation" tracks to view rRNA. You can also use the E ...
manualE6901
manualE6901

... to undergo thiol-induced cleavage at its N-terminus (3,5). The vector allows for the purification of a target protein without any extra amino acids by cloning into the NdeI and SapI sites. The target protein is fused at its C-terminus to a selfcleavable intein tag (~28 kDa) that contains the chitin ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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