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... “dominance”, but it refers to an advantage or disadvantage, not just to which allele is expressed. Overdominant alleles are alleles with codominance or incomplete dominance in which the heterozygote is better than either homozygote. Almost all commercial grain crops have overdominant alleles so that ...
Protein A gene expression is regulated by DNA supercoiling which
Protein A gene expression is regulated by DNA supercoiling which

... As the expression of the protein A gene is dramatically repressed by high salt concentrations (1 M NaCl) and as high osmolarity alters the degree of plasmid DNA supercoiling, we looked at the effect of changing the degree of DNA supercoiling on protein A gene expression. We first determined the conc ...
Analysis of Drosophila Species Genome Size and Satellite DNA
Analysis of Drosophila Species Genome Size and Satellite DNA

... Accepted for publication June 20, 2007 ABSTRACT The size of eukaryotic genomes can vary by several orders of magnitude, yet genome size does not correlate with the number of genes nor with the size or complexity of the organism. Although ‘‘whole’’-genome sequences, such as those now available for 12 ...
DNA Fingerprinting by Restriction Enzyme Patterns
DNA Fingerprinting by Restriction Enzyme Patterns

... DNA typing (also called DNA profile analysis or DNA fingerprinting) is the process whereby the genomic DNA of an organism is analyzed by examining several specific, variable DNA sequences located throughout the genome. In humans, DNA fingerprinting is now used routinely for identification purposes. Human ...
Methylation of the Factor IX Gene is the Main Source of Mutations
Methylation of the Factor IX Gene is the Main Source of Mutations

... A total of 750 mutations in the human coagulation factor IX gene in 806 patients with hemophilia В were analyzed. It was found that 40% of all point mutations occur in 11 "hot spots," which are CG methylation sites where *CG TG or *CG CA substitutions take place. A mechanism is proposed which expl ...
module 8- lecture 1 gene therapy: introduction and methods
module 8- lecture 1 gene therapy: introduction and methods

... NPTEL – Bio Technology – Genetic Engineering & Applications ...
Molecular Genetics and Genomics
Molecular Genetics and Genomics

... the other two had lost both phenotypes. Chromosomal exoB Tn5 insertions could not be obtained by following the plasmid incompatibility method (Ditta 1986). A disruption mutant of the chromosomal exoB gene was obtained by using gene sacB (Quandt and Hynes 1993), which renders cells unable to grow in ...
20Sexual Reproduction, Meiosis, and Genetic Recombination
20Sexual Reproduction, Meiosis, and Genetic Recombination

... Because sexual reproduction combines genetic information from two different parents into a single offspring, at some point in its life cycle every sexually reproducing organism has cells that contain two copies of each type of chromosome, one inherited from each parent. The two members of each chrom ...
Unit VI – Structure and Function of DNA and RNA Unit VI: Structure
Unit VI – Structure and Function of DNA and RNA Unit VI: Structure

... Water and DNA are polar molecules. Because water is a polar molecule, it is a good solvent for other polar substances. Lipids are non-polar and do not dissolve in water which is polar. Detergents have both a non-polar end (which loves the lipids) and a polar end (which loves the water), so detergent ...
HIV Epidemiology, Testing Strategies, and Prevention - IAS-USA
HIV Epidemiology, Testing Strategies, and Prevention - IAS-USA

... (Abstract 982). From 1996 to 2007, the median CD4+ cell count at first presentation increased from 234/µL to 327/µL (P < .01), with increases seen in all risk groups. Although the proportion of persons with a CD4+ cell count of at least 350/µL at first presentation also increased (34% vs 47%; P = .0 ...
functionless dna - Semantic Scholar
functionless dna - Semantic Scholar

... DNA, the order of nucleotides is under selection. Strictly, a DNA element of length l is defined as literal DNA if its function can be performed by a very small subset of the 4 ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA

... approximately 1200 bp PCR product besides the approximately 1500 bp specific product, making direct sequencing impossible. Sequence analyses gave interesting results. The reported 0.2 % difference between 16S rRNA gene sequences of type strains of R. qingshengii and R. jialingiae was not found, beca ...
Molecular evidence for the existence of additional members of the
Molecular evidence for the existence of additional members of the

... Design of primers and probes. The 16s rRNA gene was chosen as the target, since it was the only gene of which the sequence was available for all known members of the Chlamydiaceae. All sequences were downloaded from GenBank and aligned. Possible primer and probe locations compatible with all known C ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA

... and R. jialingiae shared 99.4 % gyrB gene homology, while they showed 98.6 % and 98.9 % homology with type strain of R. erythropolis (their closest relative based on the gyrB gene), respectively (Fig. 1a). The gyrB-based phylogenetic tree shows that R. qingshengii–R. jialingiae strains cluster close ...
The orientation bias of Chi sequences is a general tendency of G
The orientation bias of Chi sequences is a general tendency of G

... even higher fractions ( Fig. 3c). The orientation fractions of the three hexamers CTGGTG, TGGTGG and GCTGGT, which are composites of the Chi sequence, were also above the average (Fig. 3a). As for B. subtilis, the distributions that showed an obvious G content bias were similar to those of E. coli ( ...
Laboratory Support to HIV Diagnosis and Monitoring of Antiretroviral
Laboratory Support to HIV Diagnosis and Monitoring of Antiretroviral

... mother-to-child was 4.4%. Reports also indicate that more than 78% of the cases are in the age range of 20-39 with a male to female ratio of 3.3 to 1. HIV prevalence in pregnant women rose from 0% in 1989 to 2.3% in 1995, before decreasing to 1.5% in 1998. This rate increased again to 1.8% in 1999 a ...
DNA/RNA/Transcription/Translation Chapter CHAP 13 all reading
DNA/RNA/Transcription/Translation Chapter CHAP 13 all reading

... were enriched with different radioactive isotopes. One set of viruses had radioactive sulfur (35S) atoms attached to proteins. The other set had radioactive phosphorus (32P) atoms attached to DNA. Step 2 Second, each set of viruses was allowed to infect separate batches of nonradioactive bacteria. B ...
The Rate and Tract Length of Gene Conversion between
The Rate and Tract Length of Gene Conversion between

... the HO site in the M AT locus [39] and target site of I-Sce I endonuclease [40]) and some did not. A technical problem is that, in a simple experimental design with a single selected marker, an estimated rate may include both gene conversion and unequal crossing-over because they have an identical o ...
CDC’s Interim Guidance on the Use of
CDC’s Interim Guidance on the Use of

... Limited breastfeeding safety data on TDF/FTC Limited data re: transfer into milk compartment ...
transposon
transposon

...  Transposable elements confer neither advantage nor disadvantage on the phenotype, but could constitute “selfish DNA,” concerned only with their own propagation. ...
foreign
foreign

... Eg. Round worm in human intestine // cuscuta and higher plants (angiosperms) // hedge plants or any other suitable example = ½ Commensalism - Interaction in which one species is benefitted & the other is neither harmed nor benefitted = ½ Eg. Orchid growing as an epiphyte on mango tree branch / any o ...
Topologically Non-linked Circular Duplex DNA
Topologically Non-linked Circular Duplex DNA

... 1981). It was first observed in synthetic copolymers. It is not considered to be the structure of purified DNA in solution for any DNA obtained from natural sources, so that the presumption that DNA from natural sources can exist in the left-handed configuration is unproven. Nevertheless, we need to ...
The specificity of regulatory protein binding to DNA is due to a
The specificity of regulatory protein binding to DNA is due to a

... at the interior of an uninterrupted sequence of bonded reaction centres, S(j) is the corresponding stability constant. V=--jRT6n6' is the free energy change associated with the formation of a boundary between adjacent stretches of bonded and nonbonded ligand reaction centres ( V ^ O ) . The free ene ...
The presence of two UvrB subunits in the UvrAB complex ensures
The presence of two UvrB subunits in the UvrAB complex ensures

... signi®cant average volume. Since these unwrapped complexes are expected to have lost the ATP molecule, we decided to analyse these complexes further by determining the volumes of UvrB±DNA complexes that were isolated by washing in the absence of ATP. As expected, no wrapped complexes were observed a ...
Relaxosome function and conjugation regulation in Flike plasmids a
Relaxosome function and conjugation regulation in Flike plasmids a

... Transcription from PY, as well as the transcription of the major plasmid transcription factor, TraJ, depends on a number of host-encoded proteins which are sensitive to cellular conditions like nutrient availability and stress. Transcription from the traJ promoter, PJ, is controlled by the global tr ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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