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IB Biology 11 SL (H) - Anoka
IB Biology 11 SL (H) - Anoka

... Analyse a human karyotype to determine gender and whether non-disjunction has occurred Define genotype, phenotype, dominant allele, recessive allele, codominant alleles, locus, homozygous, heterozygous, carrier and test cross Determine the genotypes and phenotypes of the offspring of a monohybrid cr ...
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Transient cold shock enhances zinc-finger nuclease
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... target a ZFN to virtually any DNA sequence and the evolutionary conservation of the DNA repair pathways acting on ZFNinduced DNA double-stranded breaks. Simple methods that could be broadly applied to increase the cellular activity of a ZFN pair across a variety of experimental settings would facili ...
Ross - Tree Improvement Program
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BioSc 231 Exam 5 2008
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... histidine sidechains, and by hydrophobic amino acid sidechains that are buried on the inside of the folded motif. Zinc finger proteins function as regulators of gene expression. They bind to the negatively-charged backbone of DNA through a positively-charged arginine amino acid sidechain located at ...
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... A way to screen plasmid-based genome libraries for a DNA fragment of interest • Host bacteria containing a plasmid-based library of DNA fragments are plated on a petri dish and allowed to grow overnight to form colonies • Replica of dish made with a nitrocellulose disk ...
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... Specifically, biosensor experiments and structural and chemical shift analysis via NMR were used to examine the interaction between the zinc finger domain of Nup153 and the small GTPase Ran. Single zinc fingers within the zinc finger domain of Nup153 were able to bind Ran with micromolar affinity, a ...
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Genomics
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... Coli K12 could infect K12 but not E. Coli B: K12 phage growth was "restricted" in B strain. ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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