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1. Enzyme responsible for the unwinding and "unzipping" of DNA
1. Enzyme responsible for the unwinding and "unzipping" of DNA

... 11. Scientist whose pictures helped to determine the shape of DNA. 14. Scientists who built the first accurate model of DNA. 17. Number of identical strands of DNA following DNA replication. 18. The type of macromolecule which includes DNA and RNA. 19. The double stranded molecule which contains the ...
1b Unit 5 DNA structure and replication powerpoint
1b Unit 5 DNA structure and replication powerpoint

DNA extraction lab
DNA extraction lab

... 4.Gently run a teaspoonful of ice-cold ethanol into the tube. Methanol or rubbing alcohol isopropanol - should also work; make sure they are ice cold by placing the bottle in the freezer for a few hours before the experiment. Watch the point where the two layers meet. You may see strands of DNA form ...
PHYS 4xx Intro 3 1 PHYS 4xx Intro 3
PHYS 4xx Intro 3 1 PHYS 4xx Intro 3

... Genetic information is stored in the DNA sequences using the GCAT alphabet. Both the sequence for a gene, and it's complement (ie, CGTA) is stored, although other information is also encoded to indicate which is the correct direction for transcription. The sequence on the DNA master blueprint corres ...
Chapter 47
Chapter 47

... 12. A bacterial plasmid is 100 kb in length. The plasmid DNA was digested to completion with two restriction enzymes in three separate treatments: EcoRI, HaeIII, and EcoRI + HaeIII (double digest). The fragments were then separated with electrophoresis, as shown. ...
History of DNA
History of DNA

... Why is deoxyribose called a pentose sugar? ...
DNA
DNA

... • These 4 nucleotides are linked in various ways and order – creating DNA. • There are 2 strands of DNA • The sugar and the phosphate make up the backbone of the DNA. • One nucleotide from each strand come bond together to form what looks like ...
PowerPoint
PowerPoint

... Dr. MAHA SMAISM ...
Diapositive 1 - Swisstransfusion
Diapositive 1 - Swisstransfusion

... DNA and RNA sequencing “the process of determining the precise order of nucleotides within a nucleic acid molecule” DNA ...
HOW SAGE WORKS (Reference http://www
HOW SAGE WORKS (Reference http://www

DNA - Miss Gleason`s Science
DNA - Miss Gleason`s Science

... • X-ray showed strands of DNA are twisted around each other like coils • Angle of the X shows there are 2 strands ...
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... antibiotic resistance and sexual reproduction of bacteria ...
Microbial Genetics
Microbial Genetics

DNA – The Double Helix In 1952, Rosalind Franklin discovered that
DNA – The Double Helix In 1952, Rosalind Franklin discovered that

... In 1952, Rosalind Franklin discovered that DNA (deoxyribonucleic acid) is two chains of molecules in a spiral form. In 1953, using the work of Franklin and other scientists, James Watson and Francis Crick established the structure of DNA. They determined that the shape of DNA is a double helix, whic ...
Lecture #7 Date - Woodland Hills School District
Lecture #7 Date - Woodland Hills School District

Macromolecular Sequence Analysis Introduction
Macromolecular Sequence Analysis Introduction

... Environmental  DNA  is  then  sheared  into  fragments  that  are  used  in   construction  of  a  DNA  clone  library  (either  small-­ or  medium-­insert   libraries  (2-­15  kb  insert  size)  or  large-­insert  (up  to  150  kb  insert  size)),   that  may  be  sequenced  in  either  a  random   ...
Unit 3 (Chapter 20).
Unit 3 (Chapter 20).

Vocabulary Glossary - CTAE Resource Network
Vocabulary Glossary - CTAE Resource Network

... 6. DNA Ligase: Type of enzyme that can link two DNA strands together 7. EcoRI: Enzyme isolated from E. coli used as a restriction enzyme 8. Electroporation: Applying an electric current to a living surface in order to open pores through which something may pass 9. Ethidium Bromide: Fluorescent biolo ...
Document
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...  One of the basic tools of modern biotechnology is DNA restriction enzyme digestion.  This is the process of cutting DNA into smaller fragments using enzymes.  This allows scientists to analyze and compare DNA from different sources, and to study the smaller more manageable pieces of DNA.  Becau ...
Table S1: Description of the cohort used for the novel - HAL
Table S1: Description of the cohort used for the novel - HAL

... (http://evs.gs.washington.edu/EVS/). For whole genome sequencing, we used 54 individual genomes sequenced by Complete Genomics (www.completegenomics.com). For both methods, we observed a very low sequencing coverage of SHANK1 and SHANK3 (Figure S3). Whole genome sequencing seems to be a better appro ...
GLOSSARY:
GLOSSARY:

... Deionized water (dH2O) - Purified water that lacks ions such as sodium, calcium, and chloride. Denaturation - The change in a macromolecule's structure due to unfavorable conditions, such as extreme temperature pH. Denaturation is used in polymerase chain reaction (PCR) to break hydrogen bonds betwe ...
Neanderthals get in on the action - Max
Neanderthals get in on the action - Max

... protein and studied its properties. This led them to discover that the activity of a certain gene variant in the melanocortin receptor in two Neanderthals was significantly reduced. Gene variants with a similarly reduced activity are also observed in modern humans – with visible consequences: indivi ...
DNA SEQUENCING DNA sequencing
DNA SEQUENCING DNA sequencing

... DNA molecules are first attached to primers on a slide and amplified so that local clonal colonies are formed (bridge amplification). Four types of reversible terminator bases (RT-bases) are added, and non-incorporated nucleotides are washed away. Unlike pyrosequencing, the DNA can only be extended ...
What organelle is responsible for storing DNA in eukaryotic cells
What organelle is responsible for storing DNA in eukaryotic cells

... order: the human species has 23 pairs, designated 1 to 22 in order of decreasing size and X and Y for the female and male sex chromosomes respectively. • Our definition: The place where all the genes of an organisms are held. • Other forms: chromosomes, chromosomal ...
1_genomics
1_genomics

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DNA sequencing



DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a DNA sequencer, DNA sequencing has become easier and orders of magnitude faster.
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