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DNA & Protein Synthesis - Pottsgrove School District
DNA & Protein Synthesis - Pottsgrove School District

Bio1001Ch12W
Bio1001Ch12W

... • At the replication fork • the leading stand is copied ______________ from a single primer • the lagging strand is copied in ____________ using many primers. ...
BioRad #166-0007EDU: Forensic DNA Fingerprinting Checklist PREP
BioRad #166-0007EDU: Forensic DNA Fingerprinting Checklist PREP

... Restriction enzymes (endonucleases) are natural defense mechanisms of bacteria against invading bacteria. Restriction enzymes act like molecular scissors, making cuts at specific sequence of base pairs (palindromes) that it recognizes. Bacteria’s own DNA is protected by methyl groups at sites that w ...
DNA for Dummies Notes - Dr. Annette M. Parrott
DNA for Dummies Notes - Dr. Annette M. Parrott

Nucleic Acid review
Nucleic Acid review

... 29. What nitrogenous base is used in RNA in place of Thymine found in DNA? ...
Gel Electophoresis: Forensic Plasmid DNA identification
Gel Electophoresis: Forensic Plasmid DNA identification

Mutations - The Super Heroes of Biology
Mutations - The Super Heroes of Biology

Brooker Chapter 10
Brooker Chapter 10

... • It can also relax positive supercoils when they occur ...
Mitochondria are the - Charlin Manchester Terriers
Mitochondria are the - Charlin Manchester Terriers

... You've undoubtedly heard the debate before: those who insist the dam of the puppies has a greater genetic influence on them than the sire has, and those who just as adamantly insist that notion is biologically impossible. The sire and dam each contribute the same number of genes from their own DNA d ...
Is eating DNA safe?
Is eating DNA safe?

... reasonable question, but the answer seems to be In plants, different apps (genes) are on in leaves and roots but all the cells of a plant carry the same no. Imagine you dropped a smartphone in a blender or ate it (please don't) – all the components set of genes, i.e. the same genome. would be mashed ...
replication
replication

Biotechnological Tools and Techniques
Biotechnological Tools and Techniques

... AKA – Restriction Enzymes These enzymes cut DNA at specific sequences called recognition sites. The cuts at the recognition sites can be in the form of either blunt ends or sticky ends (with sticky ends being the more useful of the two). Restriction enzymes naturally occur in bacteria as a defense a ...
DNA Technology
DNA Technology

... • -when we alter/manipulate genes • For what reasons? – Cure diseases/mutations (GENE THERAPY) – Improve food crops – What else?! ...
Pipe cleaner DNA
Pipe cleaner DNA

... the thymine (green) and cytosine (yellow) pieces are folded in half. Make marks on the tape every 3 cm (15 total) for 5 codons of bases. Lay a pipe cleaner piece on the first mark perpendicular to the tape, so that it overlaps exactly one half of the tape. This step is repeated with random colors un ...
Building a DNA Model with K`nex
Building a DNA Model with K`nex

DNA Replication - SCF Faculty Site Homepage
DNA Replication - SCF Faculty Site Homepage

... • Proofreading of the newly-formed DNA is accomplished by other DNA polymerases. • Can occur AFTER replication has finished. • In this case – a Nuclease enzyme cuts out a segment containing the damaged DNA, which is then replaced by DNA Polymerase and Ligase. ...
Genetics Exam Review #2
Genetics Exam Review #2

... 2. DNA strand is unzipped between the nitrogen bases. The point at which it is unzipping is called the replication fork. 3. DNA Polymerase adds nucleotides to each side of the original DNA strand. 4. When DNA Polymerase reaches the ends of the strands, two identical copies of the DNA have been made. ...
DNA- HL sample test
DNA- HL sample test

... A biochemist isolated and purified molecules needed for DNA replication. When some DNA was added replication occurred, but the DNA molecules formed were defective. Each consisted of a normal DNA strand paired with segments of DNA a few hundred nucleotides long. Which of the following had been left o ...
document
document

... 2. The rows of the table should be labeled with characteristics found in both breeds of dogs. Examples might include size, color, type of coat, intelligence, aggression, and ...
RESTRICTION ENZYMES
RESTRICTION ENZYMES

... HOW DO RESTRICTION ENZYMES WORK? Usually cut DNA at a “palindrome” such as GAATTC. Palindrome – word or phrase when spelled backwords, spells the same word or phrase ...
Grade-Level Science Homework Due: Friday, October 7th, 2011
Grade-Level Science Homework Due: Friday, October 7th, 2011

... question is that almost everyone’s DNA (deoxyribonucleic acid) varies (varies = differs). DNA is a large organic compound located in the nucleus of eukaryotic cells, and it stores all the genetic information needed to build and operate an organism. In prokaryotic cells, the DNA floats in the cytopla ...
DNA Study Guide Answer Key
DNA Study Guide Answer Key

... 9. What is the shape of DNA? A double helix 10. How many strands of nucleotides are in DNA? 2 11. Why is DNA replication necessary and important? In order to pass exact copies to daughter cells 12. Define the following: a. Helicase: An enzyme that moves along the DNA molecules breaking hydrogen bond ...
DNA
DNA

... Purines have a two-ring structure Cytosine and Thymine are pyrimidines Pyrimidines have a one-ring structure ...
Enzyme POGIL-PCR
Enzyme POGIL-PCR

... to anneal before the Taq polymerase catalyzes the reactions to incorporated new nucleotides into the complimentary strands. The cycle is then repeated over and over until there are millions of copies of the target DNA. 3. EXPLAIN why this bacterial polymerase is used for PCR instead of human polymer ...
Chapter 13 Biotechnology 2013
Chapter 13 Biotechnology 2013

... If a species is called TRANSGENIC if it has foreign genetic material from a different species. ...
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DNA repair



DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.
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