MCB 110 Problem set 2. DNA replication - Answers
... 10. Consider Fig. 5-28 in Alberts (pg. 254) showing a mammalian DNA replication fork. How does this eukaryotic fork differ from the fork in prokaryotes? Are these differences actual (real) or do they reflect gaps in knowledge or inaccuracies in the figure? ...
... 10. Consider Fig. 5-28 in Alberts (pg. 254) showing a mammalian DNA replication fork. How does this eukaryotic fork differ from the fork in prokaryotes? Are these differences actual (real) or do they reflect gaps in knowledge or inaccuracies in the figure? ...
DNA Structure exercise v2.pptx
... 1) Explore how hydrogen bond donors and acceptors of the nucleosides(tides) influences the overall double helical structure of DNA including isosteric nature of the W-C basepairs and groove structure. 2) Predict the impact of a non-W-C pairing on a DNA double helix? Find evidence to support or ref ...
... 1) Explore how hydrogen bond donors and acceptors of the nucleosides(tides) influences the overall double helical structure of DNA including isosteric nature of the W-C basepairs and groove structure. 2) Predict the impact of a non-W-C pairing on a DNA double helix? Find evidence to support or ref ...
DNA Twizzler Model Lab - Manhasset Public Schools
... a. Refer to the table to the right to choose the correct color marshmallow to represent the chemical bases in your sequence. b. Place a marshmallow on the end of a toothpick so that the point of the toothpick goes all the way through. Stick the toothpick into the twizzler as pictured on the right. D ...
... a. Refer to the table to the right to choose the correct color marshmallow to represent the chemical bases in your sequence. b. Place a marshmallow on the end of a toothpick so that the point of the toothpick goes all the way through. Stick the toothpick into the twizzler as pictured on the right. D ...
Biotechnology - Genetic Engineering
... 3. Restriction enzyme “cuts” the DNA into many pieces every time it recognizes its specific recognition site. 4. Place DNA sample into the gel electrophoresis apparatus. 5. One end of apparatus is negative and the other is positive (like a battery). DNA is negative. ...
... 3. Restriction enzyme “cuts” the DNA into many pieces every time it recognizes its specific recognition site. 4. Place DNA sample into the gel electrophoresis apparatus. 5. One end of apparatus is negative and the other is positive (like a battery). DNA is negative. ...
Genes for Speed or Endurance?
... Fast twitch fibres fire more rapidly with more force than slow twitch fibres. Slow twitch fibres are more efficient in using oxygen to generate energy, while fast twitch fibres are less efficient in energy generation. Genetics The DNA molecule is the carrier of genetic information. Genes consist of ...
... Fast twitch fibres fire more rapidly with more force than slow twitch fibres. Slow twitch fibres are more efficient in using oxygen to generate energy, while fast twitch fibres are less efficient in energy generation. Genetics The DNA molecule is the carrier of genetic information. Genes consist of ...
FIGURE 9.2
... Proofreading by DNA polymerase (a) corrects errors during replication. In mismatch repair (b), the incorrectly added base is detected after replication. The mismatch repair proteins detect this base and remove it from the newly synthesized strand by nuclease action. The gap is now filled with the co ...
... Proofreading by DNA polymerase (a) corrects errors during replication. In mismatch repair (b), the incorrectly added base is detected after replication. The mismatch repair proteins detect this base and remove it from the newly synthesized strand by nuclease action. The gap is now filled with the co ...
Supplemental Material
... DNA manipulations, and chkA and chkB deletion strains isolation: DNA manipulations were according to SAMBROOK et al. (2001). Isolation of A. nidulans DNA was performed using standard procedures. DNA fragment probes for Southern analysis were labeled with [-32P]dCTP using the RTS Random Primer DNA l ...
... DNA manipulations, and chkA and chkB deletion strains isolation: DNA manipulations were according to SAMBROOK et al. (2001). Isolation of A. nidulans DNA was performed using standard procedures. DNA fragment probes for Southern analysis were labeled with [-32P]dCTP using the RTS Random Primer DNA l ...
DNA RNA structure
... DNA is in the nucleus. RNA is made in the nucleus but travels to the cytoplasm • RNA is made in the nucleoli but can travel out to the cytoplasm ...
... DNA is in the nucleus. RNA is made in the nucleus but travels to the cytoplasm • RNA is made in the nucleoli but can travel out to the cytoplasm ...
Biotechnology - Solon City Schools
... Bacteria as vectors • Bacteria are used because they have plasmids • A plasmid is a circular piece of DNA that exists apart from the chromosome and replicates independently of it. ...
... Bacteria as vectors • Bacteria are used because they have plasmids • A plasmid is a circular piece of DNA that exists apart from the chromosome and replicates independently of it. ...
cell division notes -
... check out animations and diagrams flash animation comparing mitosis and meiosis another animation 1. binary fission – reproduction in bacteria – p. 127 fig. 8.3 2. cell cycle p. 129 fig. 8.5 3. stages of mitosis – p. 130-131 4. cytokinesis – p. 132, fig 8.7 5. controls on the cell cycle - fig. 8.9 6 ...
... check out animations and diagrams flash animation comparing mitosis and meiosis another animation 1. binary fission – reproduction in bacteria – p. 127 fig. 8.3 2. cell cycle p. 129 fig. 8.5 3. stages of mitosis – p. 130-131 4. cytokinesis – p. 132, fig 8.7 5. controls on the cell cycle - fig. 8.9 6 ...
de novo
... and discrimination when using the modified nucleotide triphosphates and anchored DNA templates. ...
... and discrimination when using the modified nucleotide triphosphates and anchored DNA templates. ...
Lecture 6 Quiz
... 4. Which of the correct functions defined in the previous exercise is the fastest? Hint. You will need to generate a very large string to test them on, and the function clock() from the time module to time each function. ...
... 4. Which of the correct functions defined in the previous exercise is the fastest? Hint. You will need to generate a very large string to test them on, and the function clock() from the time module to time each function. ...
HLA typing of renal patients and investigation of disease
... Amplification of DNA takes place in a thermal cycler. The process involves a series of up to thirty cycles consisting of three steps. 1) The double stranded DNA is heated to 95ºC breaking the hydrogen bonds between them and separating the two strands. 2) As the temperature is reduced, the primers an ...
... Amplification of DNA takes place in a thermal cycler. The process involves a series of up to thirty cycles consisting of three steps. 1) The double stranded DNA is heated to 95ºC breaking the hydrogen bonds between them and separating the two strands. 2) As the temperature is reduced, the primers an ...
3.4 DNA Replication - hrsbstaff.ednet.ns.ca
... DNA replication is a process that copies a strand of DNA to produce a new strand with the same sequence of bases. DNA replication is semi-conservative because each new strand produced by replication contains half of the original parent strand. In other words, half the parent strand is conserved ...
... DNA replication is a process that copies a strand of DNA to produce a new strand with the same sequence of bases. DNA replication is semi-conservative because each new strand produced by replication contains half of the original parent strand. In other words, half the parent strand is conserved ...
-‐ CHROMOSOME STRUCTURE, REPLICATION, TRANSCRIPTION
... DNA REPLICATION – CELL DIVISION • DNA has to be replicated ONLY ONCE per cell division 1. DNA opens (DNA elicase) 2. Synthesis of new complementary strand ...
... DNA REPLICATION – CELL DIVISION • DNA has to be replicated ONLY ONCE per cell division 1. DNA opens (DNA elicase) 2. Synthesis of new complementary strand ...
5 POINT QUESTIONS 1. A. Give the anticodon sequences (with 5` 3
... transferring bacterial genes. ...
... transferring bacterial genes. ...
Chapter 20 Terms to Know
... Insulin is made by the pancreas. This peptide hormone (protein) ensures that glucose is taken up by the ...
... Insulin is made by the pancreas. This peptide hormone (protein) ensures that glucose is taken up by the ...
DNA Structure - Mr. Lesiuk
... So you should be able to look at DNA and determine which base is a double-ringed Purine and which are a single-ringed Pyrimidine. Then you should observe how many H-bonds are found between the bases. From the above information you should then be able to identify each base. Try it. Try this pairing ...
... So you should be able to look at DNA and determine which base is a double-ringed Purine and which are a single-ringed Pyrimidine. Then you should observe how many H-bonds are found between the bases. From the above information you should then be able to identify each base. Try it. Try this pairing ...
History_of_DNA
... E.Coli DNA polymerase I requires: 1. All four dNTPs (dATP, dGTP, dCTP and dTTP) 2. A primer chain with a free 3`-OH end 3. A template strand to which the primer is basepaired • Double-stranded DNA that is fully intact and lacking a free 3`-OH end will not be replicated (Ex: Intact circular DNA) 4. M ...
... E.Coli DNA polymerase I requires: 1. All four dNTPs (dATP, dGTP, dCTP and dTTP) 2. A primer chain with a free 3`-OH end 3. A template strand to which the primer is basepaired • Double-stranded DNA that is fully intact and lacking a free 3`-OH end will not be replicated (Ex: Intact circular DNA) 4. M ...
DNA STUDY GUIDE
... 3. Where in the cell are chromosomes located? 4. Where can DNA be found in the cell? How is each of these forms of DNA inherited? 5. What is the shape of DNA? 6. What are the “rungs” on the DNA ladder made of? 7. What sugar is found in DNA? 8. A bonds with _______ 9. C bonds with _______ 10. Where a ...
... 3. Where in the cell are chromosomes located? 4. Where can DNA be found in the cell? How is each of these forms of DNA inherited? 5. What is the shape of DNA? 6. What are the “rungs” on the DNA ladder made of? 7. What sugar is found in DNA? 8. A bonds with _______ 9. C bonds with _______ 10. Where a ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.