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DNA HISTORY NOTES
DNA HISTORY NOTES

... • Sugars and phosphates make the sides of the ladder, nitrogen bases are the rungs • The atoms within the two strands are held ...
DNA: The molecular basis of mutations
DNA: The molecular basis of mutations

... 2. External influences can create mutations Mutations can also be caused by exposure to specific chemicals or radiation. These agents cause the DNA to break down. This is not necessarily unnatural — even in the most isolated and pristine environments, DNA breaks down. Nevertheless, when the cell rep ...
DNA Structure Notes (12.1)
DNA Structure Notes (12.1)

... •DNA carries information in a triplet code. •Each sequence of 3 nucleotides codes for a certain amino acid or for the beginning or end of a sequence. •The genetic code is unique for each organism. ...
DNA is a double helix
DNA is a double helix

Obtain PCR-Ready Genomic DNA from Buccal Cells, HeLa Cells, Hair
Obtain PCR-Ready Genomic DNA from Buccal Cells, HeLa Cells, Hair

... range of sample types, requires only heating. The DNA obtained is readily amplifiable by PCR, as shown here using the FailSafe PCR System. The QuickExtract method allows for the inexpensive processing of one to hundreds of samples in less than an hour without centrifugation, spin columns, or use of ...
DNA/RNA/protSynth practicE/REVIEW quiz KEY dna_practice_quiz
DNA/RNA/protSynth practicE/REVIEW quiz KEY dna_practice_quiz

... 3- two molecules of DNA identical to each other and the original molecule are made 1- DNA “unzips” 1. What process is shown in these diagrams? ...
Study Questions
Study Questions

... 20.8. The Ti plasmid of Agrobacterium is often used to transform_____________. A) E. coli B) bacteria C) plants D) pigs E) cows 20.9. In situ hybridization can be used to: A) transfer electrons to cytochrome c B) breed plants in a common garden C) locate proteins in the mitochondria D) locate DNA po ...
Understanding DNA Technology - Southern Beef Technology Services
Understanding DNA Technology - Southern Beef Technology Services

DNA
DNA

History of DNA - Duplin County Schools
History of DNA - Duplin County Schools

... What are four sources of DNA that he used? http://fig.cox.miami.edu/~cmallery/150/gene/chargaff.htm Adenine (A) pairs with _____________ Guanine (G) pairs with _____________ ...
DNA methylation
DNA methylation

... What is epigenetic inheritance? A heritable change in gene expression that is not caused by changes in the DNA seguence → DNA methylation → Histone post-translational modifications → Histone variants → non-histone protein composition of chromatin → chromatin remodelling complexes → (RNA – antisense ...
Please mark the correct answer for each question on the separate
Please mark the correct answer for each question on the separate

... b. ligation of DNA fragments by DNA ligase d. heat denaturation of double stranded DNA ...
Lecture 6 S - BEHESHTI MAAL
Lecture 6 S - BEHESHTI MAAL

... Mutagens Ultraviolet damage – Nucleotide excision repair Enzymes cut out distorted thymines Creates gap Gap is filled with newly synthesized DNA DNA ligase joins strand to surrounding backbone ...
Document
Document

... together __________________ of both organisms. 5. What are the risks of inbreeding? ______________________________________________ 6. Scientists can increase genetic variation by inducing ______________________________ (the ultimate source of variation). 7. When organisms have double or triple the n ...
DNA BARCODING CHILLIES
DNA BARCODING CHILLIES

Southern Transfer
Southern Transfer

Everyone Needs a Repair Crew: Elizabethkingia anophelis R26
Everyone Needs a Repair Crew: Elizabethkingia anophelis R26

... interpretations of our findings. Our next task was to determine whether a gene was on or off by using the Blast database. We began this task by first navigating to the RAST database where we went to the list of our five genes and clicked on each one which would take us to a new page where we would t ...
DNA - TeacherWeb
DNA - TeacherWeb

... (twisted ladder) side/rails: sugar-phosphate backbone steps/rungs: N bases ...
Document
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... pairing of bases ...
Student Handout - University of California, Irvine
Student Handout - University of California, Irvine

... ____________ pieces of DNA will have more difficulty moving through the gel than ___________ fragments. Thus, larger fragments will move _____________ than smaller fragments. This allows separation of all different __________ of DNA fragments. 10 min. ...
DNA REPLICATION
DNA REPLICATION

... coded information for your cell is found within the base pair sequence of DNA • But in truth, where are these base pair sequences? They are in fact closed off from the outside world; remember that the structure of DNA is a double helix – it is very difficult to get to the base pairs because DNA is a ...
The Discovery of DNA
The Discovery of DNA

... • Wilkins passed on the photo that Franklin had taken to Watson and Crick. • Using Franklin’s photo and Chargaff’s rule, Watson and Crick were able to explain and prove the double helical structure of DNA. • Using Chargaff’s data, they were able to come up with the “complementary base pairing” in DN ...
The Production of a
The Production of a

... Endonucleases – enzymes that cut RNA or DNA at specific sites; restriction enzymes are endonucleases that cut DNA Sticky cells – restriction fragments in which one end of the double stranded DNA is longer than the other; necessary for the formation of recombinant DNA Restriction enzyme mapping – det ...
Lecture 7 DNA REPLICATION
Lecture 7 DNA REPLICATION

... of the newly synthesized DNA appears as short pieces(1000-2000 nt); if no ligation, short pieces will accumulate. Replication of T4 phage DNA in E. coli, wild type vs. ligase mutant, with 3Hthymidine pulse labeling (when ung- E. coli mutant was used, >50% of newly labeled DNA was still in short piec ...
6.G Meiosis Graphic Organizer 6.H Genetic Variation
6.G Meiosis Graphic Organizer 6.H Genetic Variation

... _____5. If an individual possesses two recessive alleles for the same trait, the individual is said to be a. homozygous for the trait. b. haploid for the trait c. heterozygous for the trait. d. mutated. ...
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DNA repair



DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.
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