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12.1 DNA
12.1 DNA

SAR_Gene_technology
SAR_Gene_technology

... The principle • mRNA is complimentary to the DNA in a specific gene • Reverse transcriptase is able to make a strand of DNA that is complimentary to the mRNA • If the mRNA for a specific gene is isolated then the gene can be synthesised using reverse transcriptase • The DNA formed is called complim ...
Entry task
Entry task

... • HOW DO YOU THINK SCIENTISTS WERE ABLE TO DETERMINE THAT DNA WAS THE INHERITANCE MOLECULE THAT WAS PASSED FROM PARENTS TO OFFSPRING? (12.1) ...
Recombinant DNA Using Bacterial Plasmids NAME: Background
Recombinant DNA Using Bacterial Plasmids NAME: Background

... 2. Obtain scissors and a piece of tape (your teacher will come around and give you one piece that you can further cut into smaller pieces). 3. Cut the PLASMID (White) strips. Tape the strips in ANY order to make a circular plasmid. 4. As one partner works on step 4, the other partner should cut out ...
DNAandReplication Exam Qs.doc
DNAandReplication Exam Qs.doc

... In part (i) there were some vague answers which did not gain credit. Although examiners allowed a variety of correct responses, some candidates simply offered nucleotide. This was not specific enough. A small number referred to the phosphate chain or deoxyribose backbone, showing a lack of understan ...
DNA and Mutations Webquest
DNA and Mutations Webquest

... 1. What is sickle-cell anemia? 2. People with _________ copies of the gene have the disease. 3. What are the effects of the sickle cell gene? ...
DNA: Deoxyribonucleic acid
DNA: Deoxyribonucleic acid

... They used Phosphorous – 32 because proteins have no phosphorous in them and Sulfur – 35 because DNA has almost no sulfur in it. They wanted to see which isotopes would spread into new cells and which one would not. ...
CfE Higher Biology
CfE Higher Biology

... • This strand is called the lagging strand and its formation known as discontinuous. • After both strands have been joined by their complementary bases 2 new DNA molecules are formed. ...
2.6 & 7.1 DNA & RNA Structure Notes 2.6 DNA RNA
2.6 & 7.1 DNA & RNA Structure Notes 2.6 DNA RNA

... cytoplasm ...
Restriction Enzymes
Restriction Enzymes

... Restriction enzymes are part of a bacteria's ''immune'' system. These are enzymes that cut DNA at specific sites (typically a four or a 6 base-pair sequence). Bacterial DNA is modified to be protected by methylation while foreign DNA, such as incoming viruses, are not. Usually, organisms that make ...
B. They contain the Homeobox (A unique DNA nucleotide sequence.)
B. They contain the Homeobox (A unique DNA nucleotide sequence.)

... A. The first step in this process uses Restriction Enzymes to create “Sticky Ends” on a plasmid AND DNA from other source. 1. These are enzymes that cut DNA at specific nucleotide sequences. a. This specific DNA sequence is referred to as the Restriction Site. (Fig: 20.3) 2. These enzymes create Res ...
Research Highlights: Highlights from the last year in nanomedicine
Research Highlights: Highlights from the last year in nanomedicine

DNA - Community College of Rhode Island
DNA - Community College of Rhode Island

... ◦ piece of DNA must be spliced into a plasmid  Accomplished using restriction enzymes  cut DNA at specific nucleotide sequences  cuts produce pieces of DNA called restriction fragments ...
The Molecule of Life: DNA
The Molecule of Life: DNA

... The “rungs” are made up of four bases: A, G, T, C. ...
PCR amplifies any target DNA sequence. (N)
PCR amplifies any target DNA sequence. (N)

... Doc note, I dissent. A fast never prevents a fatness. I diet on cod. ...
Harlem DNA Lab brochure
Harlem DNA Lab brochure

... Laboratory experiences for students in grades 5-8 include a variety of hands-on experiments to introduce basic genetics and molecular biology. Labs include, but are not limited to: • DNA Models (1 hour) Build a model of the double helix to better understand the genetic code. • Observing Mutant Organ ...
Chapter 20 Notes
Chapter 20 Notes

... DNA polymerase and ligase What else would be necessary for the process to work? Primers and nucleotides! How was the original DNA initially uncoiled and unwound? Heat Why did the heat cause difficulty with the procedure? ...
Key Area 2 – Pupil Booklet
Key Area 2 – Pupil Booklet

... 1. state that genes are located on chromosomes in the nucleus of every cell. 2. state that a gene is a section of DNA which controls an inherited characteristic. 3. state that genes are passed on from parents to offspring in the egg and ...
DNA, Genes and Chromosomes
DNA, Genes and Chromosomes

Chromosome Mapping by Recombination Genes on the same
Chromosome Mapping by Recombination Genes on the same

DNA
DNA

... DNA maintains continuity (composition, order) by a process called _____________________ (which means duplication) ...
Ch 13 student notes
Ch 13 student notes

... from one organism could work in a different organism. 2. Some scientists isolated the gene from fireflies and inserted it into a plant gene. The plants glowed in the dark. 3. This showed that both plants and animals use the same process to translate DNA into proteins. 4. The glowing plant is transge ...
DISCOVERY OF DNA
DISCOVERY OF DNA

... 4. Which of the following is NOT a correct structure of a nucleotide? a. adenine—deoxyribose—phosphate c. cytosine—deoxyribose—phosphate b. adenine—ribose—phosphate d. guanine—deoxyribose—phosphate 5. The percentage of adenine in DNA is a. equal to the percentage of cytosine. b. equal to the percent ...
Cell Cycle DNA Structure and Replication Student PPT Nts
Cell Cycle DNA Structure and Replication Student PPT Nts

... • ______________________: when a chunk of DNA (usually large) is removed from 1 chromosome and attached to another ...
DNA and Mutations Webquest
DNA and Mutations Webquest

... 1. What is sickle-cell anemia? 2. People with _________ copies of the gene have the disease. 3. What are the effects of the sickle cell gene? ...
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DNA repair



DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.
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