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DNA extraction lab
DNA extraction lab

... 4.Gently run a teaspoonful of ice-cold ethanol into the tube. Methanol or rubbing alcohol isopropanol - should also work; make sure they are ice cold by placing the bottle in the freezer for a few hours before the experiment. Watch the point where the two layers meet. You may see strands of DNA form ...
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... replicated as the molecule unzips but the other strand (the 3’ to 5’ side, called the lagging strand) needs to be replicated in sections then joined together later by an enzyme called DNA ligase. ...
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Name: Date: Period: ______ Must

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DNA - The Double Helix - Ms. Robbins` PNHS Science Classes

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NOTES Ch. 12 DNA

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File - High School Biology

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... An analogy is a relationship between two pairs of terms or phrases written as a : b :: c : d. The symbol : is read as “is to,” and the symbol :: is read as “as.” In the space provided, write the letter of the pair of terms or phrases that best completes the analogy shown. ...
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... mathematically related to the distance between them • The further apart 2 genes are the higher the probability of a crossing over event separating them • Recombination frequencies vary between 0% and 50% • This method is useful for establishing gene maps (location of genes on a chromosome • Example: ...
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1. Enzyme responsible for the unwinding and "unzipping" of DNA

... 8. The five-carbon sugar found in DNA and to which nitrogen bases attach. 9. The stage of the cell cycle in which DNA replication takes place. 10. The model which states that parental strands of DNA serve as a blueprint for the new, complementary strands. 11. Scientist whose pictures helped to deter ...
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Ch6.1 - Cobb Learning
Ch6.1 - Cobb Learning

... 22. The pairing of bases allows the cell to ______________________, or make copies of DNA. 6. Complement of base in question 5: 23. Both sides of a DNA molecule are ______________________, binding only with their match. ...
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Homologous recombination



Homologous recombination is a type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of DNA. It is most widely used by cells to accurately repair harmful breaks that occur on both strands of DNA, known as double-strand breaks. Homologous recombination also produces new combinations of DNA sequences during meiosis, the process by which eukaryotes make gamete cells, like sperm and egg cells in animals. These new combinations of DNA represent genetic variation in offspring, which in turn enables populations to adapt during the course of evolution. Homologous recombination is also used in horizontal gene transfer to exchange genetic material between different strains and species of bacteria and viruses.Although homologous recombination varies widely among different organisms and cell types, most forms involve the same basic steps. After a double-strand break occurs, sections of DNA around the 5' ends of the break are cut away in a process called resection. In the strand invasion step that follows, an overhanging 3' end of the broken DNA molecule then ""invades"" a similar or identical DNA molecule that is not broken. After strand invasion, the further sequence of events may follow either of two main pathways discussed below (see Models); the DSBR (double-strand break repair) pathway or the SDSA (synthesis-dependent strand annealing) pathway. Homologous recombination that occurs during DNA repair tends to result in non-crossover products, in effect restoring the damaged DNA molecule as it existed before the double-strand break.Homologous recombination is conserved across all three domains of life as well as viruses, suggesting that it is a nearly universal biological mechanism. The discovery of genes for homologous recombination in protists—a diverse group of eukaryotic microorganisms—has been interpreted as evidence that meiosis emerged early in the evolution of eukaryotes. Since their dysfunction has been strongly associated with increased susceptibility to several types of cancer, the proteins that facilitate homologous recombination are topics of active research. Homologous recombination is also used in gene targeting, a technique for introducing genetic changes into target organisms. For their development of this technique, Mario Capecchi, Martin Evans and Oliver Smithies were awarded the 2007 Nobel Prize for Physiology or Medicine.
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