DNA Structure and Replication Notes
... Determined that nucleotides (the building blocks of DNA) occur in specific ratios - Guanine = cytosine - Adenine = thymine Did not know why this was true, only that every organism followed this rule ...
... Determined that nucleotides (the building blocks of DNA) occur in specific ratios - Guanine = cytosine - Adenine = thymine Did not know why this was true, only that every organism followed this rule ...
Molecular Bio
... runs in opposite directions (Crick) – one strand runs 5’ to 3’, while the other runs 3’ to 5’ – DNA polymerase only adds nucleotides at the free 3’ end, forming new DNA strands in the 5’ to 3’ direction only ...
... runs in opposite directions (Crick) – one strand runs 5’ to 3’, while the other runs 3’ to 5’ – DNA polymerase only adds nucleotides at the free 3’ end, forming new DNA strands in the 5’ to 3’ direction only ...
Study Questions for Chapter 16: The Molecular Basis of Inheritance
... Helicase: untwist and separate DNA strands *Single-strand binding protein (protein but not an enzyme): keep DNA strands apart Primase: makes a primer (RNA nucleotides) so that DNA Polymerase II has a nucleotide to add the 1st DNA nucleotide onto (on the 3’ end of RNA nucleotide at end of primer) DNA ...
... Helicase: untwist and separate DNA strands *Single-strand binding protein (protein but not an enzyme): keep DNA strands apart Primase: makes a primer (RNA nucleotides) so that DNA Polymerase II has a nucleotide to add the 1st DNA nucleotide onto (on the 3’ end of RNA nucleotide at end of primer) DNA ...
DNA and Protein Synthesis Review Sheet
... 26. Who discovered that the shape of DNA was a double helix? Watson and Crick 27. Be able to decode a codon to an amino acid. There are several types of mutation: DELETION (a base is lost) INSERTION (an extra base is inserted) Deletion and insertion may cause what’s called a FRAMESHIFT, meaning the ...
... 26. Who discovered that the shape of DNA was a double helix? Watson and Crick 27. Be able to decode a codon to an amino acid. There are several types of mutation: DELETION (a base is lost) INSERTION (an extra base is inserted) Deletion and insertion may cause what’s called a FRAMESHIFT, meaning the ...
TruePrime™ Single Cell WGA Kit
... 1 pg of human genomic DNA (~ 1/6 of the content of one human/mammalian cell) has been amplified using either TruePrime™ (TthPrimPol-based MDA) or random primed MDA reactions. Random primed reactions contain 20% of sequences that cannot be mapped to any organism in sequence databases. ...
... 1 pg of human genomic DNA (~ 1/6 of the content of one human/mammalian cell) has been amplified using either TruePrime™ (TthPrimPol-based MDA) or random primed MDA reactions. Random primed reactions contain 20% of sequences that cannot be mapped to any organism in sequence databases. ...
DNAMocktst
... He was researching bacteria and stumbled on a transforming agent. What three scientists eventually identified the transforming agent ? What was the mystery transforming agent? ...
... He was researching bacteria and stumbled on a transforming agent. What three scientists eventually identified the transforming agent ? What was the mystery transforming agent? ...
Working with Data The Meselson–Stahl Experiment
... replication. The DNA molecules were subjected to centrifugation using a cesium chloride gradient to separate the labeled molecules based on density. After the first round of replication, all of the DNA was of intermediate density, proving the conservative model false. After the second round of repli ...
... replication. The DNA molecules were subjected to centrifugation using a cesium chloride gradient to separate the labeled molecules based on density. After the first round of replication, all of the DNA was of intermediate density, proving the conservative model false. After the second round of repli ...
DNA
... • 3.4 Angstrom between two nucleotides in a single strand and 34 Angstrom every complete turn of the helix • The two strands of the helix are 20 Angstrom apart • major groove and a minor groove alternate ...
... • 3.4 Angstrom between two nucleotides in a single strand and 34 Angstrom every complete turn of the helix • The two strands of the helix are 20 Angstrom apart • major groove and a minor groove alternate ...
The History of Molecular Genetics
... By studying pea plants, determined: – Characteristics are inherited in discrete units (later called genes) – Inherited units are independently segregated and assorted – Inherited units can be dominant or recessive ...
... By studying pea plants, determined: – Characteristics are inherited in discrete units (later called genes) – Inherited units are independently segregated and assorted – Inherited units can be dominant or recessive ...
DNA Extraction Lab
... In this investigation, you will isolate DNA from strawberries and liver. DNA is 100 000 times longer than the cell itself, but only takes up about 10% of the space in the cell. It achieves this by tight folding and packaging with proteins. DNA extraction is the first step in many biotechnological pr ...
... In this investigation, you will isolate DNA from strawberries and liver. DNA is 100 000 times longer than the cell itself, but only takes up about 10% of the space in the cell. It achieves this by tight folding and packaging with proteins. DNA extraction is the first step in many biotechnological pr ...
poster - GeoGenetics
... breaks in DNA backbones. Those groups prevent their sequencing using the HeliScope platform since they must be poly-A tailed on their free 3’-OH (Fig 1, B). Furthermore, regarding the short size of ancient templates, two denaturation temperatures were evaluated. Extracts from Pleistocene horse bones ...
... breaks in DNA backbones. Those groups prevent their sequencing using the HeliScope platform since they must be poly-A tailed on their free 3’-OH (Fig 1, B). Furthermore, regarding the short size of ancient templates, two denaturation temperatures were evaluated. Extracts from Pleistocene horse bones ...
DNA
... Base Pairing • In order to form the rungs of the ladder, the bases need to be paired • A pyrimidine and a purine are paired together – Cytosine (C) + Guanine (G) – Thymine (T) + Adenine (A) • Hydrogen bonds form between the pairs and hold them together ...
... Base Pairing • In order to form the rungs of the ladder, the bases need to be paired • A pyrimidine and a purine are paired together – Cytosine (C) + Guanine (G) – Thymine (T) + Adenine (A) • Hydrogen bonds form between the pairs and hold them together ...
Yellow Review Guide
... 2. If we think of a DNA molecule as a ladder…. Alternating _______________ and ______________ make up the sides of the ladder. The sides of the ladder are considered the “backbones” of the DNA molecule. ______________________ make up the “rungs” (middle bars) of the ladder. The “rungs” are held toge ...
... 2. If we think of a DNA molecule as a ladder…. Alternating _______________ and ______________ make up the sides of the ladder. The sides of the ladder are considered the “backbones” of the DNA molecule. ______________________ make up the “rungs” (middle bars) of the ladder. The “rungs” are held toge ...
5 POINT QUESTIONS 1. A. Give the anticodon sequences (with 5` 3
... A woman is born with Turner Syndrome (an aneuploid condition) and red-green color blindness; associated with expression of an X-linked allele. Both her parents had normal vision. Explain as fully as possible. The woman inherited the X-linked recessive allele from her mother, who was heterozygous for ...
... A woman is born with Turner Syndrome (an aneuploid condition) and red-green color blindness; associated with expression of an X-linked allele. Both her parents had normal vision. Explain as fully as possible. The woman inherited the X-linked recessive allele from her mother, who was heterozygous for ...
Southern_Hybridization2
... • Blotting paper and a stack of dry absorbent paper (such as paper towels) are then placed on the membrane • Capillary action – fluid is drawn from reservoir up through gel and into stack of dry paper. - Carries denatured DNA fragments up with it out of gel onto membrane (stick forming same pattern) ...
... • Blotting paper and a stack of dry absorbent paper (such as paper towels) are then placed on the membrane • Capillary action – fluid is drawn from reservoir up through gel and into stack of dry paper. - Carries denatured DNA fragments up with it out of gel onto membrane (stick forming same pattern) ...
Gene Technology Quest – Study Guide KEY What is a genome? A
... 1. The ingredients to make new DNA are added to PCR machine (DNA strands, DNA polymerase, DNA ligase, primers, and free nucleotides) DNA is heated in order to separate the strands. 2. Sample is cooled down and primers are added to segments in order for DNA polymerase to attach to strands. 3. DNA pol ...
... 1. The ingredients to make new DNA are added to PCR machine (DNA strands, DNA polymerase, DNA ligase, primers, and free nucleotides) DNA is heated in order to separate the strands. 2. Sample is cooled down and primers are added to segments in order for DNA polymerase to attach to strands. 3. DNA pol ...
A Unit 6 Videoscript
... your hair color gene was working in your liver? Or the information for making toenails was turned on as you were making earlobe cells? Hey, it’s pretty remarkable that we end up halfway normal at all. When cells divide, to either make a copy of themselves when you grow, or to make an egg or sperm ...
... your hair color gene was working in your liver? Or the information for making toenails was turned on as you were making earlobe cells? Hey, it’s pretty remarkable that we end up halfway normal at all. When cells divide, to either make a copy of themselves when you grow, or to make an egg or sperm ...
The controversial DNA search that helped nab the `Grim Sleeper` is
... Finally, they searched for a partial match to see whether he had a relative in the database. They got lucky — the man had a brother in custody, which led authorities to the assailant. The “Roaming Rapist” is one of a handful of cases that California authorities have quietly solved in recent years us ...
... Finally, they searched for a partial match to see whether he had a relative in the database. They got lucky — the man had a brother in custody, which led authorities to the assailant. The “Roaming Rapist” is one of a handful of cases that California authorities have quietly solved in recent years us ...
DNA – Deoxyribose Nucleic Acid
... Errors in DNA replication? Mutations can be in form of deletions, additions, and/or change of nucleotide types. Errors are usually very few – a type of DNA polymerase is involved in proof-reading and repair of mistakes. Final errors are usually about one in a billion. DNA polymerases and DNA ligase ...
... Errors in DNA replication? Mutations can be in form of deletions, additions, and/or change of nucleotide types. Errors are usually very few – a type of DNA polymerase is involved in proof-reading and repair of mistakes. Final errors are usually about one in a billion. DNA polymerases and DNA ligase ...
bio ch 8 - Saint Joseph High School
... Franklin had died in 1958 and never got credit for her accomplishment. Watson and Crick are still working in GeneticsWatson headed up the Human Genome Project that recently decoded all 3 billion ‘rungs’ of human code. ...
... Franklin had died in 1958 and never got credit for her accomplishment. Watson and Crick are still working in GeneticsWatson headed up the Human Genome Project that recently decoded all 3 billion ‘rungs’ of human code. ...
ChapteR 16 The molecular basis of inheritance
... • Eukaryotic = linear DNA molecules associated with large amounts of protein ...
... • Eukaryotic = linear DNA molecules associated with large amounts of protein ...
Experiment Methods
... Sheared DNA was purified with AmPure XP beads to further reduce polysaccharide and polyphenol contaminants. The purified DNA was end-‐‐repaired and ligated to splinkerette adaptors. Splinkerette-‐‐PCR [1] has proved very efficient for the isolation of transposon insertion sites [2]. Its design ensur ...
... Sheared DNA was purified with AmPure XP beads to further reduce polysaccharide and polyphenol contaminants. The purified DNA was end-‐‐repaired and ligated to splinkerette adaptors. Splinkerette-‐‐PCR [1] has proved very efficient for the isolation of transposon insertion sites [2]. Its design ensur ...
Make sure Welcome to Biology sheet is signed by a parent or
... During his experiment he mixed heat killed pneumonia causing bacteria with a strain of harmless bacteria and discovered that some factor is transferred between the two types to ...
... During his experiment he mixed heat killed pneumonia causing bacteria with a strain of harmless bacteria and discovered that some factor is transferred between the two types to ...
DNA profiling
DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.