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PPT File
PPT File

... ribosomes, the site of protein synthesis • only a few types of rRNA exist in cells • ribosomes consist of 60 to 65% rRNA and 35 to 40% protein • in both prokaryotes and eukaryotes, ribosomes consist of two subunits, one larger than the other • analyzed by analytical ultracentrifugation • particles c ...
DNA - The Double Helix
DNA - The Double Helix

... but how? It is the sequence of bases that determine which protein is to be made. The sequence is like a code that we can now interpret. The sequence determines which proteins are made and the proteins determine which activities will be performed. And that is how the nucleus is the control center of ...
DNA, Transcription, and Translation*.
DNA, Transcription, and Translation*.

...  How it’s done: (This happens in the Nucleus!) 1. Transcription begins with Helicase (another enzyme) binding to a region of DNA called a promoter, and then unwinding the double helix and separating a section of the 2 DNA strands 2. RNA polymerase then moves along one strand of the separate DNA lik ...
Plasmid w/ kanamycin resistance (pKAN)
Plasmid w/ kanamycin resistance (pKAN)

... • Mix plasmids with restriction enzymes – BamH1 and Hind III – Restriction enzymes cut the plasmids at precise locations ...
DNA conformation and restriction enzyme activity
DNA conformation and restriction enzyme activity

DNA: The Genetic Material
DNA: The Genetic Material

... the outside of the cell. Chargaff discovered in 1949 that in DNA, the amount of adenine always equals the amount of thymine, and the amount of cytosine always equals the amount of guanine. The X-ray diffraction photographs of DNA taken by Wilkins and Franklin in 1952 revealed a tightly coiled helix ...
Genetic Diversity Of Freshwater Snails in The Peconic River Using
Genetic Diversity Of Freshwater Snails in The Peconic River Using

DNA extraction from cheek cells protocol I mailed to you
DNA extraction from cheek cells protocol I mailed to you

... 6. Complete the following sentences to describe the structure of DNA. In the backbone of each strand in the DNA double helix molecule, the sugar of one nucleotide is bonded to the __________________ in the next nucleotide. The ________________ of the nucleotides in each strand of DNA extend toward e ...
Document
Document

... DNA molecule packed together with proteins • The bacterial chromosome is a double-stranded, circular DNA molecule associated with a small amount of protein • Eukaryotic chromosomes have linear DNA molecules associated with a large amount of ...
DNA (Deoxyribonucleic Acid)
DNA (Deoxyribonucleic Acid)

... cell extends in a single thread for almost 1-2 meters long!!! • It contains information equal to some 600,000 printed pages of 500 words each!!! (a library of about 1,000 books) ...
Export to PDF
Export to PDF

... 65% of the students will effectively communicate how they designed and carried out the experiment. ...
File
File

... on one side form one replication fork 2) Displace strand on two sides form replication forks b. Forks proceed around circle creating a daughter c. When complete, circles of DNA are present 2. Eukaryote DNA is not circular, but in a. Each chromosome has replication forks b. Each zone replicated as di ...
Product Datasheets
Product Datasheets

... product cloning. This System takes only 20-30 minutes to fuse DNA fragments to one DNA molecule. This System is recommended for the following application, but not limited to: 1. Cloning PCR products ...
Transcription / Translation Poster
Transcription / Translation Poster

... Your assignment: Use your DNA and two other colors of construction paper (one for uracil and one for ribose) to construct a transcribed model of mRNA that is complimentary to one strand of your DNA molecule. Use those models as a basis to illustrate a poster that shows transcription of mRNA. Your tr ...
My DNA RNA and Protein Notes
My DNA RNA and Protein Notes

... start the chain and DNA polymerase adds nucleotides in the 5’  3’ direction 15. The lagging strand runs (5’  3’), so a new strand built from this template strand must copy (5’  3’) heading out of the replication fork… Primase adds RNA primers at various spots as the fork opens and DNA polymerase ...
fance - Baylor College of Medicine
fance - Baylor College of Medicine

... hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. Characteristic clinical features include developmental abnormalities in major organ systems, early-onset bone marrow failure, and a high predisposition to cancer. Definitive genotype/phenotype corr ...
dna replication activity
dna replication activity

... 4. Disconnect the nucleotides on the 2nd DNA model, so that you have 12 bases to use for replication. Open one end of your remaining DNA model (one or two base at a time to each side on the original model by removing two hydrogen bonds (pretend you are helicase) Then add complementary nucleotides to ...
DNA - HCC Learning Web
DNA - HCC Learning Web

... structure consists of two DNA strands running in opposite directions, held together by complementary base pairing, and twisted into a double helix. DNA’s structure allows organisms to store and replicate the information needed to grow and reproduce. ...
DNA - The Double Helix
DNA - The Double Helix

... to build the organism. Yet, how can a heart be so different from a brain if all the cells contain the same instructions? Although much work remains in genetics, it has become apparent that a cell has the ability to turn off most genes and only work with the genes necessary to do a job. We also know ...
DNA replication
DNA replication

... The genetic code is redundant: many amino acids are encoded by more than one triplets The genetic code is „comma-free”: the triplets are not isolated units The genetic code is universal: every living being is descended from a single common ancestor Few exception: mitochondria, chloroplasts, protista ...
MICROBIAL GENETICS
MICROBIAL GENETICS

... Semi conservative which means that one DNA ...
Chapter 16
Chapter 16

... 1. How did the work of TH Morgan and company contribute to the eventual discovery that DNA was the genetic material in the cell? 2. Explain the transformation experiment conducted by Frederick Griffith. What did the results of the experiment suggest? 3. Explain how the work of Avery, McCarty, and Mc ...
Document
Document

... Name the three components of a nucleotide. Adenine pairs with _________ Guanine pairs with _________ The complimentary strand of TACGGT is ________. _________ is the scientist who used x-rays to study DNA. _________ is the scientist who found nitrogen bases occur in the same proportion. 7. _______ a ...
DNA replication
DNA replication

Chapter 12-1: DNA - SandersBiologyStuff
Chapter 12-1: DNA - SandersBiologyStuff

... waves around an edge or barrier) to see the structure of DNA. She aimed a powerful x-ray beam at DNA samples and recorded the scattering pattern of the x-rays on film. From her work she was able to see that the DNA strands were ______________________________ forming a ____________. 3. The Double Hel ...
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DNA profiling



DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.
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