The novel mutation of CYP21A2 gene and congenital adrenal
... surgery because of malformation of external genitalia. DNA sequence analysis of CYP21A2 revealed the compound heterozygous mutations (g.6119T>A and g.6699delA) in this patient and her elder sister. Interestingly, the g.6119T>A mutation is associated with a Simple Virilizing (SV) phenotype of CAH, an ...
... surgery because of malformation of external genitalia. DNA sequence analysis of CYP21A2 revealed the compound heterozygous mutations (g.6119T>A and g.6699delA) in this patient and her elder sister. Interestingly, the g.6119T>A mutation is associated with a Simple Virilizing (SV) phenotype of CAH, an ...
Bioinformatics, Data Analysis and Troubleshooting
... You measure the gene expression of 40 onion plant genes given 3 types of pesticides and 2 types of fertilizers. Estimate if there are significant differences on the expression of these genes depending of these factors. ...
... You measure the gene expression of 40 onion plant genes given 3 types of pesticides and 2 types of fertilizers. Estimate if there are significant differences on the expression of these genes depending of these factors. ...
Mutational Analysis of CRP Binding Site in the Regulatory Region of
... In E. coli and other related bacteria, cAMP receptor protein (CRP) forms a complex with cAMP, and plays an important role in the regulation of gene expression by binding to highly specific sites on DNA (Ullmann and Danchin, 1983; Webster et ai., 1988). The DNA regions of the cAMP-CRP binding sites i ...
... In E. coli and other related bacteria, cAMP receptor protein (CRP) forms a complex with cAMP, and plays an important role in the regulation of gene expression by binding to highly specific sites on DNA (Ullmann and Danchin, 1983; Webster et ai., 1988). The DNA regions of the cAMP-CRP binding sites i ...
Simultaneous mutation scanning for gross deletions
... Primers were designed to amplify all 79 exons of the DMD gene (the entire 30 UTR is not covered), two alternative promoters (purkinje and cortical) and two exons of the myelin protein zero gene (MPZ) located at 1q22 to control for whole gene deletions or duplications. In all cases, primers were desi ...
... Primers were designed to amplify all 79 exons of the DMD gene (the entire 30 UTR is not covered), two alternative promoters (purkinje and cortical) and two exons of the myelin protein zero gene (MPZ) located at 1q22 to control for whole gene deletions or duplications. In all cases, primers were desi ...
A novel de novo frame-shift mutation of the EDA gene in a
... the maxilla) (Sekiguchi et al. 2005), suggesting phenotypic heterogeneity of this syndrome. It is interesting to note that heterozygous mutation carriers of XLHED may have variable clinical features, displaying minor or moderate degrees of hypodontia, hypotrichosis, and hypohidrosis. In some familie ...
... the maxilla) (Sekiguchi et al. 2005), suggesting phenotypic heterogeneity of this syndrome. It is interesting to note that heterozygous mutation carriers of XLHED may have variable clinical features, displaying minor or moderate degrees of hypodontia, hypotrichosis, and hypohidrosis. In some familie ...
Study Guide Chapter 16- Molecular basis of Inheritance
... 3. Primase adds a short sequence of RNA nucleotides (called a primer). The primer is complementary to the DNA template strand. 4. DNA pol III adds nucleotides to 3’ end of the primer, synthesizing the new DNA strand in the 5’ 3’ direction. The DNA Pol III synthesizes the new DNA strand away from t ...
... 3. Primase adds a short sequence of RNA nucleotides (called a primer). The primer is complementary to the DNA template strand. 4. DNA pol III adds nucleotides to 3’ end of the primer, synthesizing the new DNA strand in the 5’ 3’ direction. The DNA Pol III synthesizes the new DNA strand away from t ...
Development of a codominant PCR-based marker for the wheat Wx
... producing udon noodles have a low amylose level due to a lack of Wx-B1 protein conditioned by the Wx-B1 null allele. It was previously determined that the entire coding region of the wheat Wx-B1 gene is deleted in the most common null allele. However, the extent and breakpoints of the deletion have ...
... producing udon noodles have a low amylose level due to a lack of Wx-B1 protein conditioned by the Wx-B1 null allele. It was previously determined that the entire coding region of the wheat Wx-B1 gene is deleted in the most common null allele. However, the extent and breakpoints of the deletion have ...
The 2013 Thomas Hunt Morgan Medal Thomas Douglas
... efficient homologous recombination in this system, provided a tool to modify the yeast genome in very precise and predetermined manners (Rothstein 1991). Tom cleverly exploited this new technology to construct defined recombination substrates, publishing a seminal study of meiotic recombination betwee ...
... efficient homologous recombination in this system, provided a tool to modify the yeast genome in very precise and predetermined manners (Rothstein 1991). Tom cleverly exploited this new technology to construct defined recombination substrates, publishing a seminal study of meiotic recombination betwee ...
incidence and detection of aviadenoviruses of serotypes 1 and 5 in
... described in September 2007 (9). In the clinical case of inclusion body hepatitis (IBH), the first isolated serotype was adenovirus serotype 5. However, serotypes 2 and 8 were the most commonly isolated serotypes from the cases of IBH (11, 17). For this reason, a molecular diagnostic technique based ...
... described in September 2007 (9). In the clinical case of inclusion body hepatitis (IBH), the first isolated serotype was adenovirus serotype 5. However, serotypes 2 and 8 were the most commonly isolated serotypes from the cases of IBH (11, 17). For this reason, a molecular diagnostic technique based ...
R5C - Royal Society of Chemistry
... Apart from any fair dealing for the purposes of research or private study, or criticism or review, as permitted under the UK Copyright Designs and Patents Act, 1988, this publication may not be reproduced, stored, or transmitted, in any form or by any means, without the prior permission in writing o ...
... Apart from any fair dealing for the purposes of research or private study, or criticism or review, as permitted under the UK Copyright Designs and Patents Act, 1988, this publication may not be reproduced, stored, or transmitted, in any form or by any means, without the prior permission in writing o ...
module three
... Most horse genes have far more DNA in them than is actually needed to code for the amino acids in their polypeptide products. Within the coding DNA, known as exons, are stretches of non-coding DNA, called introns. Many genes are composed mostly of introns. When transcription occurs all of the DNA ba ...
... Most horse genes have far more DNA in them than is actually needed to code for the amino acids in their polypeptide products. Within the coding DNA, known as exons, are stretches of non-coding DNA, called introns. Many genes are composed mostly of introns. When transcription occurs all of the DNA ba ...
Slide 1 - Cal State LA - Instructional Web Server
... -hemi-nested PCR: targets NP gene, however, mismatches with primers and published sequences and long turnaround times -Seeplex RT-PCR kit: targets M gene, however, at time of outbreak manufacturer had not disclosed M primer sequence -CDC kit: targets M gene, however, did not become available until l ...
... -hemi-nested PCR: targets NP gene, however, mismatches with primers and published sequences and long turnaround times -Seeplex RT-PCR kit: targets M gene, however, at time of outbreak manufacturer had not disclosed M primer sequence -CDC kit: targets M gene, however, did not become available until l ...
DNA Tribes Digest for May 31, 2011
... populations are relatively small, contrasted with larger contributions from geographically proximate indigenous populations of Mexico. This suggests that despite linguistic links with Western Canada and Alaska, Athabaskan speaking populations4 retain substantial connections with other peoples neighb ...
... populations are relatively small, contrasted with larger contributions from geographically proximate indigenous populations of Mexico. This suggests that despite linguistic links with Western Canada and Alaska, Athabaskan speaking populations4 retain substantial connections with other peoples neighb ...
The development of restriction analysis and PCR
... methods, using agarose gel electrophoresis to approximate DNA fragment lengths. Standard curves were constructed to allow approximation of fragment lengths (and thus plasmid identity) using the proposed methods. The efficacy of the restriction analysis and PCR-based techniques was confirmed, with th ...
... methods, using agarose gel electrophoresis to approximate DNA fragment lengths. Standard curves were constructed to allow approximation of fragment lengths (and thus plasmid identity) using the proposed methods. The efficacy of the restriction analysis and PCR-based techniques was confirmed, with th ...
Use of Alternative Primers for Gender Discrimination in Human
... determination loci will expand our repertoire of robust primers for use in forensic sample identification. This is important because of the null-Y mutation that results in a deletion of the amelogenin gene located at 6.79Mbp on the Y chromosome.3 4 5 The mutation occurs especially within certain Ind ...
... determination loci will expand our repertoire of robust primers for use in forensic sample identification. This is important because of the null-Y mutation that results in a deletion of the amelogenin gene located at 6.79Mbp on the Y chromosome.3 4 5 The mutation occurs especially within certain Ind ...
De novo Structure Variations of the Y Chromosome in a 47,XXY
... was then used to detect the CNVs in the genome of the patient. Two large deletions were identified on the short arm of the Y chromosome (fig. 1c). Deletion 1 was ∼6 Mb in size and spanned Yp11.32 to Yp11.2 (positions from the gene chip data: 179,542–6,110,498 bp; hg19) and included the genes ZBED1, ...
... was then used to detect the CNVs in the genome of the patient. Two large deletions were identified on the short arm of the Y chromosome (fig. 1c). Deletion 1 was ∼6 Mb in size and spanned Yp11.32 to Yp11.2 (positions from the gene chip data: 179,542–6,110,498 bp; hg19) and included the genes ZBED1, ...
A Comparative Study on the Yield of DNA Extracted from Fresh
... fluids such as saliva and perspiration. Mitochondrial DNA, which follows the maternal line of an individual, can be extracted from hair and bone samples. This can be used to examine relatedness and common ancestry between individuals, and to verify the identity of buried remains. This technique was ...
... fluids such as saliva and perspiration. Mitochondrial DNA, which follows the maternal line of an individual, can be extracted from hair and bone samples. This can be used to examine relatedness and common ancestry between individuals, and to verify the identity of buried remains. This technique was ...
DNA Extraction - Utah Agriculture in the Classroom
... 18.What can be done with my extracted DNA? This sample could be used for gel electrophoresis, for example, but all you will see is a smear. The DNA you have extracted is genomic, meaning that you have the entire collection of DNA from each cell. Unless you cut the DNA with restriction enzymes, it ...
... 18.What can be done with my extracted DNA? This sample could be used for gel electrophoresis, for example, but all you will see is a smear. The DNA you have extracted is genomic, meaning that you have the entire collection of DNA from each cell. Unless you cut the DNA with restriction enzymes, it ...
(S) tet Resistance Determinant Element Containing the Tetracycline
... ECL Direct Nucleic Acid Labeling and Detection system (Amersham Biosciences, Little Chalfont, United Kingdom). Southern blots were probed with pAM120 (Table 1) and PCR products derived from tet(S), tet(M), and the int and xis genes of Tn916 (Table 1). PCR assays were also carried out as previously d ...
... ECL Direct Nucleic Acid Labeling and Detection system (Amersham Biosciences, Little Chalfont, United Kingdom). Southern blots were probed with pAM120 (Table 1) and PCR products derived from tet(S), tet(M), and the int and xis genes of Tn916 (Table 1). PCR assays were also carried out as previously d ...
Reversible supramolecular assembly at specific DNA sites: Ni
... responsiveness to external stimuli (metal ions), and even reversibility. The strategy, which relies on a planned, dual role for nickel (II) as both a dimerizing agent and folding promoter, involves a programmed self-assembly of dissimilar components. Design and synthesis: As starting point for our d ...
... responsiveness to external stimuli (metal ions), and even reversibility. The strategy, which relies on a planned, dual role for nickel (II) as both a dimerizing agent and folding promoter, involves a programmed self-assembly of dissimilar components. Design and synthesis: As starting point for our d ...
Recombinant DNA Technology
... • Contains long terminal repeats on its ends? • Generates direct repeats as a result of transposition? • Carries a gene for reverse transcriptase? • Can insert a copy in a new location while leaving the old copy at the original site? ...
... • Contains long terminal repeats on its ends? • Generates direct repeats as a result of transposition? • Carries a gene for reverse transcriptase? • Can insert a copy in a new location while leaving the old copy at the original site? ...
BYB2 - TheAllPapers
... Callus tissue develops into either shoots or roots depending on the relative concentration of the plant growth regulators used. Use your knowledge of genes to suggest how these plant growth regulators determine the type of plant tissue formed. ...
... Callus tissue develops into either shoots or roots depending on the relative concentration of the plant growth regulators used. Use your knowledge of genes to suggest how these plant growth regulators determine the type of plant tissue formed. ...
Four-color DNA sequencing by synthesis using cleavable
... identify many bases in parallel, allowing the simultaneous sequencing of a large number of DNA molecules. We have previously established the feasibility of performing SBS on a chip using four photocleavable fluorescent nucleotide analogues (26) and discovered that an allyl group can be used as a cle ...
... identify many bases in parallel, allowing the simultaneous sequencing of a large number of DNA molecules. We have previously established the feasibility of performing SBS on a chip using four photocleavable fluorescent nucleotide analogues (26) and discovered that an allyl group can be used as a cle ...
11357_2014_9648_MOESM1_ESM
... DNA methylation in response to WY14,643-treatment was found on the additional CpGs confirming present in the promoter of the FABP4 gene again confirming the 450K BeadChip results. Bisulfite conversion and pyrosequencing procedure 300ng of genomic DNA for each sample was bisulfite-treated using EZ-96 ...
... DNA methylation in response to WY14,643-treatment was found on the additional CpGs confirming present in the promoter of the FABP4 gene again confirming the 450K BeadChip results. Bisulfite conversion and pyrosequencing procedure 300ng of genomic DNA for each sample was bisulfite-treated using EZ-96 ...
Protein Synthesis: Transcription and Translation
... 1. Place your constructed DNA molecule on your desk. 2. Construct 9 mRNA nucleotides. A nucleotide of mRNA consists of a phosphate, ribose sugar, and one of four bases (A, U, C, or G). 3. Unzip your molecule of DNA. 4. On your data sheet, list the sequence of bases found along the left side of your ...
... 1. Place your constructed DNA molecule on your desk. 2. Construct 9 mRNA nucleotides. A nucleotide of mRNA consists of a phosphate, ribose sugar, and one of four bases (A, U, C, or G). 3. Unzip your molecule of DNA. 4. On your data sheet, list the sequence of bases found along the left side of your ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.