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compute1
compute1

... comparison of the two input sequences • Local Alignment looks for local stretches of similarity and tries to align the most similar segments • Algorithms used may be similar, but output different, statistics needed to assess results ...
Reproductive Epigenetics: Preparing the Epigenome for the Next
Reproductive Epigenetics: Preparing the Epigenome for the Next

... occurs between generations; it is thought that this occurs for the purpose of resetting the control of gene expression patterns and in order to prohibit the inheritance of aberrant epigenetic information prior to the start of embryo or germ cell development. The two major epigenomic reprogramming p ...
Molecular Identification and Evolutionary Relationship of the New
Molecular Identification and Evolutionary Relationship of the New

... A total of 15 specimens of the Callistethus sp.7VF-2014 were collected from different parts in Duhok Governorate during period extended from May to July 2015. The present study was done for molecular identification and evolutionary relationships according to DNA sequence and RNA secondary structure ...
Unit VI Structure and Function of DNA/RNA Teaching Module B-4
Unit VI Structure and Function of DNA/RNA Teaching Module B-4

... relationship between DNA, genes and chromosomes and demonstrate how genes and chromosomes function. Following the introduction, students should complete the Chromosome Packing worksheet (Instructional Planning Activity Guide B-4.2a, p 20) as an analysis of genes and chromosomes. Students should be a ...
Finding Regulatory Motifs
Finding Regulatory Motifs

... Sites (TFBS), also known as motifs, which are specific for a given transcription factor. • TFs influence gene expression by binding to a specific TFBS. • A TFBS can be located anywhere within the regulatory region. • TFBS may vary slightly across different regulatory regions since non-essential base ...
DNA & Protein Synthesis
DNA & Protein Synthesis

... world to map the entire gene sequence of organisms. ...
Nair, B.G. and H.S. Chhatpar
Nair, B.G. and H.S. Chhatpar

... new mutations which belong to two complementation groups are closely linked to the wc loci already mapped (Perkins et al., 1982 Microbial. Rev. 46: 426-570), and to estimate the degree of linkage between mutations. Strains of mt a and A containing the seven mutations in complementation group wc-1 we ...
Habitat management and the use of plant-based
Habitat management and the use of plant-based

... female was introduced in each plastic box for three hours at room temperature and was observed every 10 min. Only those individuals that had been seen with the stylet inserted into the leaf at least three times were considered to have fed and were frozen until tested by PCR. Lepidopteran feeding tri ...
Synthetic chromosome arms function in yeast and
Synthetic chromosome arms function in yeast and

... Recent advances in DNA synthesis technology have enabled the construction of novel genetic pathways and genomic elements, furthering our understanding of system-level phenomena1–7. The ability to synthesize large segments of DNA allows the engineering of pathways and genomes according to arbitrary s ...
replication v 2015_21
replication v 2015_21

... – And then adds correct nucleotide and proceeds down the chain again in the 5’  3’ direction ...
A Long-Term Evolutionary Pressure on the Amount of Noncoding DNA
A Long-Term Evolutionary Pressure on the Amount of Noncoding DNA

... On the one hand, variability is a prerequisite for evolvability, the ability to innovate (Wagner and Altenberg 1996; Kirschner and Gerhart 1998; Radman et al. 1999; Burch and Chao 2000; Wagner 2005). On the other hand, the long-term evolutionary success also requires that a sufficient proportion of ...
File - Molecular Biology 2
File - Molecular Biology 2

... recombinant minichromosome is introduced into E. coli cells where it replicates to produce many copies of the recombinant DNA molecule. Although the entire procedure is often referred to as the recombinant DNA or gene-cloning technique, these terms actually refer to two separate steps in the proces ...
Sample Course Material - Sevas Educational Society, Visakhapatnam
Sample Course Material - Sevas Educational Society, Visakhapatnam

... and is encoded in the DNA (or, for some viruses, RNA). Genomes can be represented as base pairs (AT or CG) of nucleic acids. Human Genome consists of 3 Billion Base Pairs. etc……. ...
Protein Synthesis
Protein Synthesis

... Proteins are made in the ribosomes! ...
Dating the Origin of the CCR5-Δ32 AIDS
Dating the Origin of the CCR5-Δ32 AIDS

... Estimating the age of CCR-32 • Stephens et al. present an equation to calculate the age of the CCR-32 mutation based on it’s level of LD • Assuming the mutation was unique, at time zero it will be in complete LD with the alleles at the neighboring loci • With an estimate of the rate of recombinat ...
Identification of Bacterial Species Using Colony PCR
Identification of Bacterial Species Using Colony PCR

... DNA is visualized in the gel using a DNA binding stain. The stain can either be incorporated into the gel itself or applied post-gel electrophoresis. Stains can be viewed using a UV light. Ethidium bromide was the most commonly used until it was found to be extremely toxic because it can easily soak ...
Design of a High School Laboratory: `Visualizing DNA Sequences`
Design of a High School Laboratory: `Visualizing DNA Sequences`

... the sequence into a protein sequence by hand and with an online tool, finding similar proteins and finding the function of the protein. o Part 2: MATLAB activities to show how technology can enhance the field of Bioinformatics and make a scientist’s work easier, faster, more accurate and more depend ...
Lecture 2
Lecture 2

... a) Space-filling model of B DNA, the most common form of DNA in cells. The sugar and phosphate residues (gray) in each strand form the backbone, which is traced by a red line, showing the helical twist of the overall molecule. The bases project inward, but are accessible through major and minor groo ...
Mutations and Genetic Variability 1. What is occurring in the diagram
Mutations and Genetic Variability 1. What is occurring in the diagram

... different chromosome. The type of chromosome translocation shown in the diagram is known as reciprocal translocation, which involves the exchange of material between two chromosomes. Reciprocal translocations are the most common type of translocation and do not result in a loss of genetic informatio ...
DNA SEQUENCING (using a Li
DNA SEQUENCING (using a Li

... methods, and their derivations. Both methods were first described in 1977. The first method (Maxam and Gilbert 1977) is based on specific chemical degradation of the DNA. The DNA is first end-labeled using 35s or 33P, followed by separation of the two strands on a gel. Four aliquots of the desired s ...
From Gene to Protein—Transcription and Translation
From Gene to Protein—Transcription and Translation

... (tRNA), is required to ensure that the correct amino acid is brought in for each codon in the mRNA. There are multiple different types of tRNA. Each type of tRNA molecule has three nucleotides that form an anti-codon. The three nucleotides in the tRNA anti-codon are complementary to the three nucleo ...
PPT1
PPT1

... • Collect all known sequences that bind a certain TF. • Align all sequences (using multiple sequence alignment). • Compute the frequency of each nucleotide in each position (PSPM). • Incorporate background frequency for each nucleotide (PSSM). ...
Drosophila
Drosophila

... • Transposes as DNA without an RNA intermediate? • Contains long terminal repeats on its ends? • Generates direct repeats as a result of transposition? • Carries a gene for reverse transcriptase? • Can insert a copy in a new location while leaving the old copy at the original site? ...
HSV 1 & 2 - Scioto County Medical Society
HSV 1 & 2 - Scioto County Medical Society

... are defined by the nucleotide base sequences on their DNA or RNA.  For our purposes, each unique DNA or RNA sequence is loosely defined as a “target.” ...
DNA Mutation and Repair
DNA Mutation and Repair

... • Spontaneous mutations • DNA polymerase has a proofreading activity that normally keeps mutation rates low but accidents happen and • Some types of mutation are invisible to the polymerase • Strand slippage is common in repetitive regions of the genome • DNA forms a temporary hairpin • DNA slips al ...
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Microsatellite



A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.
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