499 Med Chem Chap 6 problems
... 24) To which of the following does adenine form hydrogen bonds in DNA? a. Adenine. b. Thymine. c. Cytosine. d. Guanine. 25) To which of the following does cytosine form hydrogen bonds in DNA? a. Adenine. b. Thymine. c. Cytosine. d. Guanine. 26) Which of the following statements is not true about DNA ...
... 24) To which of the following does adenine form hydrogen bonds in DNA? a. Adenine. b. Thymine. c. Cytosine. d. Guanine. 25) To which of the following does cytosine form hydrogen bonds in DNA? a. Adenine. b. Thymine. c. Cytosine. d. Guanine. 26) Which of the following statements is not true about DNA ...
DNA Replication
... Lagging Strand Segments • ________ ________ - series of short segments on the lagging strand • Must be joined together by an _______ DNA ...
... Lagging Strand Segments • ________ ________ - series of short segments on the lagging strand • Must be joined together by an _______ DNA ...
![[4] Fologea, D., Brandin, E., Uplinger, J., Branton, D., Li, J](http://s1.studyres.com/store/data/022017731_1-3d28463eccad2ba4cd92d75bb9791a40-300x300.png)
[4] Fologea, D., Brandin, E., Uplinger, J., Branton, D., Li, J
... kbp or longer, and, we had not demonstrated directly that the DNA molecules were translocated through the Ion Beam Sculpted solid state nanopores. In this experiment, we provide such a proof using a ~1000 base pair long dsDNA. SI.2a. The DNA used. We used a 1 kbp linear double strand DNA segment tha ...
... kbp or longer, and, we had not demonstrated directly that the DNA molecules were translocated through the Ion Beam Sculpted solid state nanopores. In this experiment, we provide such a proof using a ~1000 base pair long dsDNA. SI.2a. The DNA used. We used a 1 kbp linear double strand DNA segment tha ...
Forensics Glossary
... Angle of impact—angle formed by the direction of a blood drop and the plane of the surface it strikes Antemortem—before death Anthropometry—method of identification, devised by Alphonse Bertillon in the late 19th century, consisting of a set of body measurements thought to form a unique Profile; obs ...
... Angle of impact—angle formed by the direction of a blood drop and the plane of the surface it strikes Antemortem—before death Anthropometry—method of identification, devised by Alphonse Bertillon in the late 19th century, consisting of a set of body measurements thought to form a unique Profile; obs ...
Crafting Super Hero Powers
... By making exact copies of DNA, and then going through mitosis and cytokinesis, eukaryotic organisms can ensure that they have the exact same DNA (and the exact same instructions) in every cell in their body. But what is actually done with those instructions? The instructions coded in your DNA are us ...
... By making exact copies of DNA, and then going through mitosis and cytokinesis, eukaryotic organisms can ensure that they have the exact same DNA (and the exact same instructions) in every cell in their body. But what is actually done with those instructions? The instructions coded in your DNA are us ...
CLARK LAP Wednesday March 26 2014 STRAWBERRY DNA
... through the cheesecloth and into the tall glass until there is very little liquid left in the funnel (only wet pulp remains). How does the filtered strawberry liquid look? • Pour the filtered strawberry liquid from the tall glass into the small glass jar so that the jar is one quarter full. • Measur ...
... through the cheesecloth and into the tall glass until there is very little liquid left in the funnel (only wet pulp remains). How does the filtered strawberry liquid look? • Pour the filtered strawberry liquid from the tall glass into the small glass jar so that the jar is one quarter full. • Measur ...
Chapter 31 - faculty at Chemeketa
... Thymine and adenine are complementary bases in DNA; therefore each time one appears, its complement appears. There is no fixed relationship between the amount of thymine and the amount of guanine, since they are not complementary. Table 31.2 shows that the one set of bases is often far different fro ...
... Thymine and adenine are complementary bases in DNA; therefore each time one appears, its complement appears. There is no fixed relationship between the amount of thymine and the amount of guanine, since they are not complementary. Table 31.2 shows that the one set of bases is often far different fro ...
No Slide Title
... 7. Final report: 30 points Assignment 1 Pick an enzyme/organism combination • Try to convince the group in 5-10 minutes why yours is best ...
... 7. Final report: 30 points Assignment 1 Pick an enzyme/organism combination • Try to convince the group in 5-10 minutes why yours is best ...
Restriction enzymes
... • A person’s DNA profile as seen on an electrophoresis gel usually shows two lines for each of the STRs tested. This is because usually, the STRs inherited from the parents are of different lengths. Occasionally, only one line appears because both STRs in a pair are of the same length. • When the D ...
... • A person’s DNA profile as seen on an electrophoresis gel usually shows two lines for each of the STRs tested. This is because usually, the STRs inherited from the parents are of different lengths. Occasionally, only one line appears because both STRs in a pair are of the same length. • When the D ...
Assurance
... Section III-D (experiments falling into any of the following categories below) Experiments Using Risk Group 2, Risk Group 3, Risk Group 4, or Restricted Agents as Host-Vector Systems Section III-D-1-a. Experiments involving the introduction of recombinant DNA into Risk Group 2 agents will usually b ...
... Section III-D (experiments falling into any of the following categories below) Experiments Using Risk Group 2, Risk Group 3, Risk Group 4, or Restricted Agents as Host-Vector Systems Section III-D-1-a. Experiments involving the introduction of recombinant DNA into Risk Group 2 agents will usually b ...
DNA Methylation Mechanisms and Analysis Methods to
... • Primers do not contain any CpG sites, but do contain non-CpG cytosines • Bisulfite-specific primer pair: amplifies both bisulfite-converted methylated and bisulfite converted unmethylated DNA • Wildtype-specific primer pair: amplifies unconverted DNA, both methylated or unmethylated sequences ...
... • Primers do not contain any CpG sites, but do contain non-CpG cytosines • Bisulfite-specific primer pair: amplifies both bisulfite-converted methylated and bisulfite converted unmethylated DNA • Wildtype-specific primer pair: amplifies unconverted DNA, both methylated or unmethylated sequences ...
The full-length HIV-1 molecular clone pLAI 61 was used to produce
... capped G residue. Supplementary Table 1 lists all oligonucleotides used in this study. The tat exon 1 was amplified by PCR on pLAI with primers NotI-WdV005 and WdV002; tat exon 2 with primers WdV007 and NotI-WdV004; rev exon 1 with primers NotI-WdV001 and WdV002; rev exon 2 with primers WdV003 and N ...
... capped G residue. Supplementary Table 1 lists all oligonucleotides used in this study. The tat exon 1 was amplified by PCR on pLAI with primers NotI-WdV005 and WdV002; tat exon 2 with primers WdV007 and NotI-WdV004; rev exon 1 with primers NotI-WdV001 and WdV002; rev exon 2 with primers WdV003 and N ...
No Slide Title
... • aatg aatg aatg aatg aatg aatg aatg aatg aatg aatg - 10 • aatg aatg aatg aatg aatg aatg atg aatg aatg aatg - 9.3 ...
... • aatg aatg aatg aatg aatg aatg aatg aatg aatg aatg - 10 • aatg aatg aatg aatg aatg aatg atg aatg aatg aatg - 9.3 ...
dna extraction - Medical Research Council
... »» Set a timer for 5 minutes. – Use this time to discuss DNA or combine with Zebra Fish Activity Plan. »» Proceed to step 4 The soap and heat have done their job to break the cell apart and release the DNA. Now invite participants to use pipettes/droppers to add pineapple juice – this will pull away ...
... »» Set a timer for 5 minutes. – Use this time to discuss DNA or combine with Zebra Fish Activity Plan. »» Proceed to step 4 The soap and heat have done their job to break the cell apart and release the DNA. Now invite participants to use pipettes/droppers to add pineapple juice – this will pull away ...
DNA Profile 911 - Bertino Forensics
... When trying to identify someone from their DNA, their physical appearance is not being examined. Instead, personal identification is based on their genes or alleles for different STRs. The more STRs being examined, the more reliable is the identification of the person. Today, DNA is analyzed using t ...
... When trying to identify someone from their DNA, their physical appearance is not being examined. Instead, personal identification is based on their genes or alleles for different STRs. The more STRs being examined, the more reliable is the identification of the person. Today, DNA is analyzed using t ...
22 Illumina library production, MITOBIM,MITOS
... login email without @unm.edu pw localgalaxy go to shared data select AdemaClass2016 select fastq and reference, import into history (name history) go to analyze data left pane NGS: QC and manipulations ...
... login email without @unm.edu pw localgalaxy go to shared data select AdemaClass2016 select fastq and reference, import into history (name history) go to analyze data left pane NGS: QC and manipulations ...
Promoter Analysis for Intestinally
... a. The sequences of all hits were extracted and flipped to the strand that maximized As and Gs. b. The sequences were then run through ClustalW. Alignments can be seen in the following file: i. C. elegans : Cele_all_hits_aligned.txt ii. C. briggsae: Cbri_all_hits_aligned.txt iii. C. remanei : Crem_a ...
... a. The sequences of all hits were extracted and flipped to the strand that maximized As and Gs. b. The sequences were then run through ClustalW. Alignments can be seen in the following file: i. C. elegans : Cele_all_hits_aligned.txt ii. C. briggsae: Cbri_all_hits_aligned.txt iii. C. remanei : Crem_a ...
Nuclear Genome Sequence Survey of the
... surveys to analyse cDNAs. To complement this, there are some studies of the physical properties of dinoflagellate chromosomes, but no direct survey of the nature of the sequences contained within them. To start to build a picture of the contents of these genomes, we have sequenced over 230,000 bp fr ...
... surveys to analyse cDNAs. To complement this, there are some studies of the physical properties of dinoflagellate chromosomes, but no direct survey of the nature of the sequences contained within them. To start to build a picture of the contents of these genomes, we have sequenced over 230,000 bp fr ...
Powerpoint slides - SEAS - The George Washington University
... - basic assumption: secondary structure of a functional RNA will be conserved in the evolution of the molecule (at least more conserved than the primary structure); when a set of homologous sequences has a certain structure in common, this structure can be deduced by comparing the structures possibl ...
... - basic assumption: secondary structure of a functional RNA will be conserved in the evolution of the molecule (at least more conserved than the primary structure); when a set of homologous sequences has a certain structure in common, this structure can be deduced by comparing the structures possibl ...
CHNOPS Document
... mRNA leaves the nucleus and travels to the ribosome where proteins are made. The 3-base codons in the mRNA strand will pair up with anticodons on the transfer RNA (tRNA) molecules. Each tRNA carries an amino acid to the ribosome, and these amino acids link together to form a protein. The process i ...
... mRNA leaves the nucleus and travels to the ribosome where proteins are made. The 3-base codons in the mRNA strand will pair up with anticodons on the transfer RNA (tRNA) molecules. Each tRNA carries an amino acid to the ribosome, and these amino acids link together to form a protein. The process i ...
A Genetic Linkage Map for the Zebrafish
... Because the mapping of cloned genes facilitates the identification of candidate genes for mutations, we developed a strategy to map genes known by DNA sequence. In this approach, DNA from each haploid of the linkage map cross serves as a template for a PCR with the use of a pair of primers that ampl ...
... Because the mapping of cloned genes facilitates the identification of candidate genes for mutations, we developed a strategy to map genes known by DNA sequence. In this approach, DNA from each haploid of the linkage map cross serves as a template for a PCR with the use of a pair of primers that ampl ...
little piggy
... Sue was busy doing what scientists do most of the time. She was waiting for something to happen. In this case the “what” was a sequencing gel. The phone rang. It was an old friend of hers currently working at the meat processing plant in town. He had a problem for her. He needed a way to determine i ...
... Sue was busy doing what scientists do most of the time. She was waiting for something to happen. In this case the “what” was a sequencing gel. The phone rang. It was an old friend of hers currently working at the meat processing plant in town. He had a problem for her. He needed a way to determine i ...
DNA polymerase I
... around the circle until they have opened and copied the entire chromosome Replicon- contains an origin and is replicated as a unit ...
... around the circle until they have opened and copied the entire chromosome Replicon- contains an origin and is replicated as a unit ...
Optimized Methods for Gene Disruption Using CRISPR/Cas9 Editing
... transfection reagents, we consistently observed poor transfection efficiency of large plasmids which express both Cas9 and the targeting sgRNA (efficiencies of 5‐40% were seen, even in easy‐to‐transfect HEK293 cells where short plasmids can give 90% efficiency). The Cas9 plasmid was decreased in siz ...
... transfection reagents, we consistently observed poor transfection efficiency of large plasmids which express both Cas9 and the targeting sgRNA (efficiencies of 5‐40% were seen, even in easy‐to‐transfect HEK293 cells where short plasmids can give 90% efficiency). The Cas9 plasmid was decreased in siz ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.