Non-destructive DNA extraction methods that yield DNA barcodes in
... Qiagen DNeasy kit (Qiagen, 2006). The DNA extracted from these spiders could be entered into a national database, such as GenBank or the Consortium for the Barcode of Life (CBOL), in order to identify existing species and confirm new species. Non-destructive DNA extraction techniques needed to be ex ...
... Qiagen DNeasy kit (Qiagen, 2006). The DNA extracted from these spiders could be entered into a national database, such as GenBank or the Consortium for the Barcode of Life (CBOL), in order to identify existing species and confirm new species. Non-destructive DNA extraction techniques needed to be ex ...
DNA Probes with Different Specificities from a Cloned
... techniques allow rapid analysis of pure and mixed cultures. It should also be possible to identify organisms that are difficult or impossible to cultivate. Ribosomal RNA genes are especially useful for the construction of specific DNA probes for various groups such as the Pseudomonas JIuorescens gro ...
... techniques allow rapid analysis of pure and mixed cultures. It should also be possible to identify organisms that are difficult or impossible to cultivate. Ribosomal RNA genes are especially useful for the construction of specific DNA probes for various groups such as the Pseudomonas JIuorescens gro ...
Structure, stability and function of 5-chlorouracil
... considered to be carcinogenic (5). 5-Iodouracil (IU) was shown to have lethal and mutagenic effects on bacteriophage T4 (6). Halogenated uracil residues are expected to exhibit base pairing properties in double-stranded nucleic acids that are closely related to those of thymine, thus involving compl ...
... considered to be carcinogenic (5). 5-Iodouracil (IU) was shown to have lethal and mutagenic effects on bacteriophage T4 (6). Halogenated uracil residues are expected to exhibit base pairing properties in double-stranded nucleic acids that are closely related to those of thymine, thus involving compl ...
ABG701 and Intro 706
... strand of DNA. The structure also suggested a simple method for duplication: if the strands are separated, new partner strands can be reconstructed for each based on the sequence of the old strand. Although the structure of DNA showed how inheritance worked, it was still not known how DNA influenced ...
... strand of DNA. The structure also suggested a simple method for duplication: if the strands are separated, new partner strands can be reconstructed for each based on the sequence of the old strand. Although the structure of DNA showed how inheritance worked, it was still not known how DNA influenced ...
Identifying the Genetic Material
... The 35S-labeled and 32P-labeled phages were used to infect two separate batches of E. coli bacteria. Because radioactive elements release particles that can be detected with machines, they can be followed, or traced, in a biological process. Scientists could determine whether it was the DNA, the pro ...
... The 35S-labeled and 32P-labeled phages were used to infect two separate batches of E. coli bacteria. Because radioactive elements release particles that can be detected with machines, they can be followed, or traced, in a biological process. Scientists could determine whether it was the DNA, the pro ...
Function of E. coli RNA Polymerase Factor 70 in
... since a few sequence changes in the early transcribed segment, particularly just after the final T, can convert a 210 consensus-induced pause at 125 of phage 82 to an arrested complex (Yarnell and Roberts, unpublished data). Second, there is a background of short transcripts extending to about 118 t ...
... since a few sequence changes in the early transcribed segment, particularly just after the final T, can convert a 210 consensus-induced pause at 125 of phage 82 to an arrested complex (Yarnell and Roberts, unpublished data). Second, there is a background of short transcripts extending to about 118 t ...
Ilot CpG
... DNMT3B, which are bound to nucleosomes containing methylated DNA99. Enhancers tend to be CpG-poor and show incomplete methylation, suggesting a dynamic process of methylation or demethylation occurs, perhaps owing to the presence of ten-eleven translocation (TET) proteins in these regions, although ...
... DNMT3B, which are bound to nucleosomes containing methylated DNA99. Enhancers tend to be CpG-poor and show incomplete methylation, suggesting a dynamic process of methylation or demethylation occurs, perhaps owing to the presence of ten-eleven translocation (TET) proteins in these regions, although ...
Protein Function
... DNA. As a protein binds to DNA some of these ions are released, and some bound water molecules are released from both the protein and the DNA as well. The release of ions and water has both positive and negative effects on the association of a protein with DNA. The positive effects come from a gener ...
... DNA. As a protein binds to DNA some of these ions are released, and some bound water molecules are released from both the protein and the DNA as well. The release of ions and water has both positive and negative effects on the association of a protein with DNA. The positive effects come from a gener ...
The specificity of regulatory protein binding to DNA is due to a
... DNA structure^" . Therefore, it seems plausible that the recognition is based on the direct correspondence between the sequence of AT- and GC-specific reaction centres on the protein surface and base pair sequence in the corresponding control site on DNA. The most advantageous conditions for protein ...
... DNA structure^" . Therefore, it seems plausible that the recognition is based on the direct correspondence between the sequence of AT- and GC-specific reaction centres on the protein surface and base pair sequence in the corresponding control site on DNA. The most advantageous conditions for protein ...
Making sense of deep sequencing
... been transformative in two ways that are both strongly tied to throughput. First, NGS enables the sequencing of large regions of genomes and even whole genomes by the efforts of one person, and by one run on one machine rather than via the work of hundreds of people, hundreds of machines and thousan ...
... been transformative in two ways that are both strongly tied to throughput. First, NGS enables the sequencing of large regions of genomes and even whole genomes by the efforts of one person, and by one run on one machine rather than via the work of hundreds of people, hundreds of machines and thousan ...
Part 1
... expression is generated by PCR and encodes the protein of interest, a T7 promoter, sequences for initiation of translation, suitable termination sequences as well as an N- or C-terminal tag sequence for immobilization onto the microarray surface. 2. Cell-free lysate: Crude cell lysates obtained from ...
... expression is generated by PCR and encodes the protein of interest, a T7 promoter, sequences for initiation of translation, suitable termination sequences as well as an N- or C-terminal tag sequence for immobilization onto the microarray surface. 2. Cell-free lysate: Crude cell lysates obtained from ...
Nucleic Acids - Farmasi Unand
... enzymes directly responsible for the formation of nucleic acids or the variety of enzymes that catalyse the various stages in the formation of the pirimidine and purine bases required for the formation of nucleic acids. prof. aza ...
... enzymes directly responsible for the formation of nucleic acids or the variety of enzymes that catalyse the various stages in the formation of the pirimidine and purine bases required for the formation of nucleic acids. prof. aza ...
Bubble dynamics in DNA
... along the DNA, whereas larger bubbles become localized in AT-rich regions [7]. Finally, upon heating, dsDNA exhibits denaturation bubbles of increasing size and number, and eventually the two strands separate altogether in a process called denaturation transition or melting [8, 9]. Depending on the ...
... along the DNA, whereas larger bubbles become localized in AT-rich regions [7]. Finally, upon heating, dsDNA exhibits denaturation bubbles of increasing size and number, and eventually the two strands separate altogether in a process called denaturation transition or melting [8, 9]. Depending on the ...
Factorindependent transcription pausing caused by recognition of
... core (Figure 2A). Complexes were similar to those used in our and other studies previously (Sidorenkov et al, 1998; Yuzenkova et al, 2010), and are based on the sequence of the transcribed region of the T7A1 promoter (the initial sequence will be referred to as ‘wild type’, wt). Complexes were assem ...
... core (Figure 2A). Complexes were similar to those used in our and other studies previously (Sidorenkov et al, 1998; Yuzenkova et al, 2010), and are based on the sequence of the transcribed region of the T7A1 promoter (the initial sequence will be referred to as ‘wild type’, wt). Complexes were assem ...
Chapter 13 - Bellbrook
... animals with desired traits. Genetic engineering can be used to increase or decrease the expression of specific genes in selected organisms. It has many applications from human health to agriculture. To understand the impact of genetic engineering, it is helpful to understand the basic tools involve ...
... animals with desired traits. Genetic engineering can be used to increase or decrease the expression of specific genes in selected organisms. It has many applications from human health to agriculture. To understand the impact of genetic engineering, it is helpful to understand the basic tools involve ...
DNA Fingerprinting by Restriction Enzyme Patterns
... base pairs. A restriction enzyme having a 6-base pair recognition site, such as Eco RI, would be expected to cut human DNA into approximately 750,000 different fragments. ...
... base pairs. A restriction enzyme having a 6-base pair recognition site, such as Eco RI, would be expected to cut human DNA into approximately 750,000 different fragments. ...
Identification of Specific DNA Binding Residues in the
... To analyze the predicted structural properties of the TCP domain, secondary elements, solvent accessibility, conformational disorder, and DNA binding properties were studied using various prediction methods. Secondary structure prediction analysis for the core DNA binding domain (Figure 2C) suggeste ...
... To analyze the predicted structural properties of the TCP domain, secondary elements, solvent accessibility, conformational disorder, and DNA binding properties were studied using various prediction methods. Secondary structure prediction analysis for the core DNA binding domain (Figure 2C) suggeste ...
PDF
... and the appearance of NHEJ hallmarks in the fusion points [15– 17,34]. We tested this hypothesis in this study. Numts inserted from the mitochondria potentially could be inserted by homologous recombination between chromosomal and mitochondrial DNA. If this is true, numt-flanking regions should show ...
... and the appearance of NHEJ hallmarks in the fusion points [15– 17,34]. We tested this hypothesis in this study. Numts inserted from the mitochondria potentially could be inserted by homologous recombination between chromosomal and mitochondrial DNA. If this is true, numt-flanking regions should show ...
Electronic Fingerprints of DNA Bases on Graphene
... and graphene plane. These peaks are marked by the colored shaded regions in the curves of Figure 3a−d. In Figure 3e, we summarize these angle dependent results for the positive and negative bias energies for all four bases. By examining Figure 3, one can recognize the dominant STS peak features, exc ...
... and graphene plane. These peaks are marked by the colored shaded regions in the curves of Figure 3a−d. In Figure 3e, we summarize these angle dependent results for the positive and negative bias energies for all four bases. By examining Figure 3, one can recognize the dominant STS peak features, exc ...
Transcription Flip-Book
... or mRNA that specifies a particular amino acid or termination signal and that functions as the basic unit of the genetic code. double Helix: The form of native DNA, referring to its two adjacent polynucleotide strands wound into a spiral shape. DNA: (Deoxyribonucleic acid) A doublestranded, helical ...
... or mRNA that specifies a particular amino acid or termination signal and that functions as the basic unit of the genetic code. double Helix: The form of native DNA, referring to its two adjacent polynucleotide strands wound into a spiral shape. DNA: (Deoxyribonucleic acid) A doublestranded, helical ...
The coiled-coil of the human Rad50 DNA repair protein contains
... For polymers like DNA, this method yields an average persistence length that is in good agreement with values obtained by other techniques (8). This method has been modified to describe known segments of different persistence length (9). Another method for the determination of overall flexibility of ...
... For polymers like DNA, this method yields an average persistence length that is in good agreement with values obtained by other techniques (8). This method has been modified to describe known segments of different persistence length (9). Another method for the determination of overall flexibility of ...
The legal, social and ethical controversy of the collection and
... He developed a technique to examine the length variation of these DNA repeat sequences, thus creating the ability to perform identification tests [8, pp.2f]. The smallest building block of DNA is known as the nucleotide. Each nucleotide contains a deoxyribose, a phosphate group and a base. When we a ...
... He developed a technique to examine the length variation of these DNA repeat sequences, thus creating the ability to perform identification tests [8, pp.2f]. The smallest building block of DNA is known as the nucleotide. Each nucleotide contains a deoxyribose, a phosphate group and a base. When we a ...
Chapter 6: DNA Replication and Telomere Maintenance I
... B. DNA Synthesis: Addition of Nucleotides to a Growing DNA Strand 1. For all organisms, DNA synthesis occurs in the 5’ 3’ direction a. Nucleotides (dNTP) are added onto the 3’ end of the growing strand with new phosphodiester bonds being formed b. In the condensation reaction, the β and γ phospha ...
... B. DNA Synthesis: Addition of Nucleotides to a Growing DNA Strand 1. For all organisms, DNA synthesis occurs in the 5’ 3’ direction a. Nucleotides (dNTP) are added onto the 3’ end of the growing strand with new phosphodiester bonds being formed b. In the condensation reaction, the β and γ phospha ...
Helicases - Maintenance
... unidirectionally along ssDNA and unwind dsDNA. They play a role in every aspect of DNA (RNA) metabolism ...
... unidirectionally along ssDNA and unwind dsDNA. They play a role in every aspect of DNA (RNA) metabolism ...
DNA nanotechnology
DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. Researchers in the field have created static structures such as two- and three-dimensional crystal lattices, nanotubes, polyhedra, and arbitrary shapes, as well as functional devices such as molecular machines and DNA computers. The field is beginning to be used as a tool to solve basic science problems in structural biology and biophysics, including applications in crystallography and spectroscopy for protein structure determination. Potential applications in molecular scale electronics and nanomedicine are also being investigated.The conceptual foundation for DNA nanotechnology was first laid out by Nadrian Seeman in the early 1980s, and the field began to attract widespread interest in the mid-2000s. This use of nucleic acids is enabled by their strict base pairing rules, which cause only portions of strands with complementary base sequences to bind together to form strong, rigid double helix structures. This allows for the rational design of base sequences that will selectively assemble to form complex target structures with precisely controlled nanoscale features. A number of assembly methods are used to make these structures, including tile-based structures that assemble from smaller structures, folding structures using the DNA origami method, and dynamically reconfigurable structures using strand displacement techniques. While the field's name specifically references DNA, the same principles have been used with other types of nucleic acids as well, leading to the occasional use of the alternative name nucleic acid nanotechnology.