9.18 Metal Complexes as Drugs and Chemotherapeutic
... interactions. Human serum albumin is of fundamental importance in the transport of drugs, metabolites, endogenous ligands, and metal ions.25 The crystal structure, combined with physical and biophysical studies, allows visualization of likely binding sites of many potential inorganic drugs.26 Abunda ...
... interactions. Human serum albumin is of fundamental importance in the transport of drugs, metabolites, endogenous ligands, and metal ions.25 The crystal structure, combined with physical and biophysical studies, allows visualization of likely binding sites of many potential inorganic drugs.26 Abunda ...
Understanding Molecular Pathology and the Recent
... face each other The two chains in the double helix are connected by hydrogen bonds Watson-Crick base-pairing rules – Adenine always pairs with thymine (uracil) – Guanine always pairs with cytosine GC base pairs (bps) have more energy than AT bps Since one strand of DNA is complementary to the other, ...
... face each other The two chains in the double helix are connected by hydrogen bonds Watson-Crick base-pairing rules – Adenine always pairs with thymine (uracil) – Guanine always pairs with cytosine GC base pairs (bps) have more energy than AT bps Since one strand of DNA is complementary to the other, ...
Infertility and aneuploidy in mice lacking a type IA
... animals, over time and through successive generations, particularly in the male germ cells. Therefore, we examined the meiotic metaphases in infertile top3⫺/⫺ males and in TOP3⫹/⫹ control animals by fluorescence in situ hybridization. A mixture of differently tagged probes that specifically hybrid ...
... animals, over time and through successive generations, particularly in the male germ cells. Therefore, we examined the meiotic metaphases in infertile top3⫺/⫺ males and in TOP3⫹/⫹ control animals by fluorescence in situ hybridization. A mixture of differently tagged probes that specifically hybrid ...
Nuclear DNA Content Estimates in Green Algal Lineages
... Streptophyta superimposed on a contemporary molecular phylogeny indicate that early diverging lineages, including some members of Chlorokybales, Coleochaetales and Klebsormidiales, have genomes as small as 0.1 – 0.5 pg. It is proposed that the streptophyte ancestral nuclear genome common to both the ...
... Streptophyta superimposed on a contemporary molecular phylogeny indicate that early diverging lineages, including some members of Chlorokybales, Coleochaetales and Klebsormidiales, have genomes as small as 0.1 – 0.5 pg. It is proposed that the streptophyte ancestral nuclear genome common to both the ...
Unit VI – Structure and Function of DNA and RNA Unit VI: Structure
... The DNA is stretched out in the nucleus as chromatin before cell division so that it can be read. DNA is the blueprint for the synthesis of proteins, and proteins determine the traits of an organism. Proteins are made up of amino acids. Each amino acid is determined by a sequence of 3 base pairs of ...
... The DNA is stretched out in the nucleus as chromatin before cell division so that it can be read. DNA is the blueprint for the synthesis of proteins, and proteins determine the traits of an organism. Proteins are made up of amino acids. Each amino acid is determined by a sequence of 3 base pairs of ...
Graph-based Methods for the Design of DNA - TUBdok
... DNA computing is a rapidly evolving field utilizing DNA molecules instead of silicon-based electronic units to perform calculations. The reliability of such computations strongly depends on the DNA sequences that represent units of information. Recently, the thermodynamic constraints, based on the f ...
... DNA computing is a rapidly evolving field utilizing DNA molecules instead of silicon-based electronic units to perform calculations. The reliability of such computations strongly depends on the DNA sequences that represent units of information. Recently, the thermodynamic constraints, based on the f ...
Charge Transport in DNA - Insights from
... DNA molecules between the electrodes were breaking. The fundamental features of CT in DNA have been described as well. First, the exponential dependence of CT efficiency on the length of the DNA oligomers. Second, the role of experimental setup that is both crucial and challenging to characterize. In ...
... DNA molecules between the electrodes were breaking. The fundamental features of CT in DNA have been described as well. First, the exponential dependence of CT efficiency on the length of the DNA oligomers. Second, the role of experimental setup that is both crucial and challenging to characterize. In ...
Indirect Recognition in Sequence
... WATCAA starting near the center of the site and the second sequence TTR located 4 bp in the 3⬘ direction from WATCAA (9). Some IHF sites also contain a poly(A)-tract containing 4 – 6 adenines and located 8 –9 bp in the 5⬘ direction from the WATCAA element (12, 13). Despite a nonrandom distribution o ...
... WATCAA starting near the center of the site and the second sequence TTR located 4 bp in the 3⬘ direction from WATCAA (9). Some IHF sites also contain a poly(A)-tract containing 4 – 6 adenines and located 8 –9 bp in the 5⬘ direction from the WATCAA element (12, 13). Despite a nonrandom distribution o ...
Hydrogen bond geometry in DNA–minor groove
... Overall, hydrogen bonds in this sample deviate significantly from a linear geometry (Table 3), with average values of 2.32 Å for the H···A distance and 124 for the N–H···A angle. Typical, linear, hydrogen bonds show H···A distances into the 1.9–2.0 Å range and N–H···A angles close to 160 (24). Thi ...
... Overall, hydrogen bonds in this sample deviate significantly from a linear geometry (Table 3), with average values of 2.32 Å for the H···A distance and 124 for the N–H···A angle. Typical, linear, hydrogen bonds show H···A distances into the 1.9–2.0 Å range and N–H···A angles close to 160 (24). Thi ...
Rosalind Franklin and the Double Helix
... The smaller single-ringed pyrimidines, cytosine (C) and thymine (T), are always paired with larger double-ringed purines, guanine (G) and adenine (A). Indeed, the consistent pairing of G with C and of A with T, as first proposed by Watson, explains the identical size of the ladder rungs and also Erw ...
... The smaller single-ringed pyrimidines, cytosine (C) and thymine (T), are always paired with larger double-ringed purines, guanine (G) and adenine (A). Indeed, the consistent pairing of G with C and of A with T, as first proposed by Watson, explains the identical size of the ladder rungs and also Erw ...
Mutation Detection by Single Strand Conformation Polymorphism
... in the past the importance of temperature on the conformational changes of the DNA fragments. It is conceivable that the known temperature effect on the stabilization of the secondary structure of singlestranded DNA fragments may affect to a varying degree (depending on the primary sequence) the SSC ...
... in the past the importance of temperature on the conformational changes of the DNA fragments. It is conceivable that the known temperature effect on the stabilization of the secondary structure of singlestranded DNA fragments may affect to a varying degree (depending on the primary sequence) the SSC ...
Gene organization, transcription signals and processing of the
... antisense strand and hybridized with total cellular RNA to reveal an band of about 4000 nucleotides (data not shown); we inferred, therefore, that the gene is expressed. The putative gene is preceded by an A-T rich sequences although none was unambiguously identified as a promoter (Fig. 2). Moreover ...
... antisense strand and hybridized with total cellular RNA to reveal an band of about 4000 nucleotides (data not shown); we inferred, therefore, that the gene is expressed. The putative gene is preceded by an A-T rich sequences although none was unambiguously identified as a promoter (Fig. 2). Moreover ...
Field and laboratory methods for DNA studies on deep−sea isopod
... verse taxa, but also represent one of the better known groups inhabiting the deep sea (Rex and Etter 2010). Their correct classification is important for evolutionary, ecological, and biogeographic studies but also for conservation issues as industrial pressures on the deep−sea environment grow (Gla ...
... verse taxa, but also represent one of the better known groups inhabiting the deep sea (Rex and Etter 2010). Their correct classification is important for evolutionary, ecological, and biogeographic studies but also for conservation issues as industrial pressures on the deep−sea environment grow (Gla ...
Select this.
... • DNA is appropriate for nanotechnological methods for several reasons: It is a (relatively) stable chemical, which exists in different forms (nucleotides, nucleic acids) As a polymer it can form very long molecules It has a well defined, repetitive structure “Rules” for determining the structur ...
... • DNA is appropriate for nanotechnological methods for several reasons: It is a (relatively) stable chemical, which exists in different forms (nucleotides, nucleic acids) As a polymer it can form very long molecules It has a well defined, repetitive structure “Rules” for determining the structur ...
Unit 8 - OpenWetWare
... Griffith called this process transformation because one strain of bacteria (the harmless strain) had changed permanently into another (the disease-causing strain). ...
... Griffith called this process transformation because one strain of bacteria (the harmless strain) had changed permanently into another (the disease-causing strain). ...
Solving a Crime Using DNA Analysis and Chemistry
... The manager of the college food services promised the president of the college a plate of his favorite chocolate chip cookies for the upcoming meeting with the executive council of the Board of Directors. The chocolate chip cookies are a specialty of Chef Lombardo's and are famous across the college ...
... The manager of the college food services promised the president of the college a plate of his favorite chocolate chip cookies for the upcoming meeting with the executive council of the Board of Directors. The chocolate chip cookies are a specialty of Chef Lombardo's and are famous across the college ...
1 Introduction - Wiley-VCH
... that are present on one molecule can fold back onto each other and form partial double stranded RNA stretches. Single stranded regions are looped out and socalled hairpins (also referred to as stem-loop structures) are formed (Figure 1.7). The double stranded portion is termed the stem and the singl ...
... that are present on one molecule can fold back onto each other and form partial double stranded RNA stretches. Single stranded regions are looped out and socalled hairpins (also referred to as stem-loop structures) are formed (Figure 1.7). The double stranded portion is termed the stem and the singl ...
the mass spectrometry-based method EpiTYPER
... amplicon sequence, look for the Excel file named after this tab delimited file with the extension: “T Report” and open this file. 22. This file contains the sequences and masses of each so-called “CpG unit”, that is a fragment containing one or multiple CpG sites. The CpG sites are numbered from the ...
... amplicon sequence, look for the Excel file named after this tab delimited file with the extension: “T Report” and open this file. 22. This file contains the sequences and masses of each so-called “CpG unit”, that is a fragment containing one or multiple CpG sites. The CpG sites are numbered from the ...
A Highly Efficient Method for the Construction of a Plasmid
... library. The new method was based on the addition of complementary single stranded oligomers to cDNA and vector as 5' overhangs and annealing of the 5' overhangs before ligation to increase ligation efficiency. To generate the long, complementary 5' overhangs to cDNA and vector DNA a common un phosp ...
... library. The new method was based on the addition of complementary single stranded oligomers to cDNA and vector as 5' overhangs and annealing of the 5' overhangs before ligation to increase ligation efficiency. To generate the long, complementary 5' overhangs to cDNA and vector DNA a common un phosp ...
The Art of DNA Strings: Sixteen Years of DNA Coding Theory
... 1) Variable Neighborhood Search Approach: This approach employs different local search algorithms to search the DNA codes [48]–[51]. Some of the search algorithms are described here. a) Seed Building(SB) : Seed Building (SB) algorithm examines all the possible codewords randomly with respect to seed ...
... 1) Variable Neighborhood Search Approach: This approach employs different local search algorithms to search the DNA codes [48]–[51]. Some of the search algorithms are described here. a) Seed Building(SB) : Seed Building (SB) algorithm examines all the possible codewords randomly with respect to seed ...
Validation of picogram- and femtogram-input DNA libraries
... Over the last decade, advances in high-throughput sequencing technologies have accelerated the exploration of the uncultured microbial majority (Rappe & Giovannoni, 2003). The direct sequencing of environmental samples, termed metagenomics, has revolutionized microbial ecology by providing new insig ...
... Over the last decade, advances in high-throughput sequencing technologies have accelerated the exploration of the uncultured microbial majority (Rappe & Giovannoni, 2003). The direct sequencing of environmental samples, termed metagenomics, has revolutionized microbial ecology by providing new insig ...
Non-homologous end-joining factors of Saccharomyces cerevisiae
... Possesses 5 0 to 3 0 exonuclease activity on its own; however, upon complex formation with, and subsequent phosphorylation by, DNA-PKcs it acquires endonucleolytic activity on 5 0 and 3 0 overhangs, as well as hairpins Involved in nucleolytic end-processing step of NHEJ Interacts with DNA-PKcs ...
... Possesses 5 0 to 3 0 exonuclease activity on its own; however, upon complex formation with, and subsequent phosphorylation by, DNA-PKcs it acquires endonucleolytic activity on 5 0 and 3 0 overhangs, as well as hairpins Involved in nucleolytic end-processing step of NHEJ Interacts with DNA-PKcs ...
13 The Klenow fragment of DNA polymerase I Pol I actually appears
... T7 is an E. coli bacteriophage (virus) that encodes its own DNA polymerase. Phage T7 polymerase is a small, single polypeptide enzyme (gp5, ~80 kDa) that contains a highly active 3'→ 5' exonuclease activity in addition to a 5'→ 3' polymerase activity. It is the enzyme responsible for replicating the ...
... T7 is an E. coli bacteriophage (virus) that encodes its own DNA polymerase. Phage T7 polymerase is a small, single polypeptide enzyme (gp5, ~80 kDa) that contains a highly active 3'→ 5' exonuclease activity in addition to a 5'→ 3' polymerase activity. It is the enzyme responsible for replicating the ...
High-Throughput NGS Library Preparation
... KAPA HTP Library Preparation Kit Technical Data Sheet (TDS) can be carried out manually, and most protocol development and validation work is usually done this way. Although it is possible to achieve moderately high sample throughput by using multi-channel pipettes and 96-well plates, automated liqu ...
... KAPA HTP Library Preparation Kit Technical Data Sheet (TDS) can be carried out manually, and most protocol development and validation work is usually done this way. Although it is possible to achieve moderately high sample throughput by using multi-channel pipettes and 96-well plates, automated liqu ...
CORPORATE RESPONSIBILITY AT DNA CORPORATE
... Customer satisfaction in DNA’s fixed-network data services among corporate customers was also better than competing operators’ for the second year in a row. ...
... Customer satisfaction in DNA’s fixed-network data services among corporate customers was also better than competing operators’ for the second year in a row. ...
DNA nanotechnology
DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. Researchers in the field have created static structures such as two- and three-dimensional crystal lattices, nanotubes, polyhedra, and arbitrary shapes, as well as functional devices such as molecular machines and DNA computers. The field is beginning to be used as a tool to solve basic science problems in structural biology and biophysics, including applications in crystallography and spectroscopy for protein structure determination. Potential applications in molecular scale electronics and nanomedicine are also being investigated.The conceptual foundation for DNA nanotechnology was first laid out by Nadrian Seeman in the early 1980s, and the field began to attract widespread interest in the mid-2000s. This use of nucleic acids is enabled by their strict base pairing rules, which cause only portions of strands with complementary base sequences to bind together to form strong, rigid double helix structures. This allows for the rational design of base sequences that will selectively assemble to form complex target structures with precisely controlled nanoscale features. A number of assembly methods are used to make these structures, including tile-based structures that assemble from smaller structures, folding structures using the DNA origami method, and dynamically reconfigurable structures using strand displacement techniques. While the field's name specifically references DNA, the same principles have been used with other types of nucleic acids as well, leading to the occasional use of the alternative name nucleic acid nanotechnology.