Phylogenomics of type II DNA topoisomerases
... All these data suggest that Topo IIA and Topo IIB enzymes have been created independently by the assembly of several modules, some of them being common (the Bergerat fold, the CAP-like domain and the toprim domain), but other being unrelated. Thus, the Topo II activity has been most likely invented ...
... All these data suggest that Topo IIA and Topo IIB enzymes have been created independently by the assembly of several modules, some of them being common (the Bergerat fold, the CAP-like domain and the toprim domain), but other being unrelated. Thus, the Topo II activity has been most likely invented ...
Chapter 11 Slides
... • Small polynucleotide chains - 73 to 94 residues each • Several bases usually methylated • Each a.a. has at least one unique tRNA which carries the a.a. to the ribosome • 3'-terminal sequence is always CCA-a.a. • Aminoacyl tRNA molecules are the substrates of protein synthesis Copyright © 1999 by H ...
... • Small polynucleotide chains - 73 to 94 residues each • Several bases usually methylated • Each a.a. has at least one unique tRNA which carries the a.a. to the ribosome • 3'-terminal sequence is always CCA-a.a. • Aminoacyl tRNA molecules are the substrates of protein synthesis Copyright © 1999 by H ...
An Introduction to Genetic Engineering: Third Edition
... 1.1 What is genetic engineering? Progress in any scientific discipline is dependent on the availability of techniques and methods that extend the range and sophistication of experiments that may be performed. Over the past 35 years or so this has been demonstrated in a spectacular way by the emergenc ...
... 1.1 What is genetic engineering? Progress in any scientific discipline is dependent on the availability of techniques and methods that extend the range and sophistication of experiments that may be performed. Over the past 35 years or so this has been demonstrated in a spectacular way by the emergenc ...
Sequence dependence of isothermal DNA amplification via EXPAR
... the interaction becomes stabilized upon polymerase extension. The newly formed trigger, however, readily dissociates from the template, which facilitates rapid amplification. In previous studies (data not shown), we found that if the trigger-template melting temperature is lower than 35 C, trigger-t ...
... the interaction becomes stabilized upon polymerase extension. The newly formed trigger, however, readily dissociates from the template, which facilitates rapid amplification. In previous studies (data not shown), we found that if the trigger-template melting temperature is lower than 35 C, trigger-t ...
Lecture 1. Introduction
... • Different DNA molecules differ only in the base, so we can represent a DNA strand simply by a string with the alphabet {A, C, G, T} Image credit: Wikipedia Last update: 2-Jan-2017 ...
... • Different DNA molecules differ only in the base, so we can represent a DNA strand simply by a string with the alphabet {A, C, G, T} Image credit: Wikipedia Last update: 2-Jan-2017 ...
Turning h Cro into a Transcriptional Activator
... equally tightly to 0~1 and 0~2. In order to facilitate assays of activation by Cro67, we introduced four base changes into OR2 that make its sequence more like that of 0~1 (Figure 3); Figure 4 shows that Cro67 bound to 0~1 and the modified OR2 equally tightly. None of these operator changes altered ...
... equally tightly to 0~1 and 0~2. In order to facilitate assays of activation by Cro67, we introduced four base changes into OR2 that make its sequence more like that of 0~1 (Figure 3); Figure 4 shows that Cro67 bound to 0~1 and the modified OR2 equally tightly. None of these operator changes altered ...
pptx - Department of Computer Science and Engineering
... • Different DNA molecules differ only in the base, so we can represent a DNA strand simply by a string with the alphabet {A, C, G, T} Image credit: Wikipedia Last update: 4-Jan-2015 ...
... • Different DNA molecules differ only in the base, so we can represent a DNA strand simply by a string with the alphabet {A, C, G, T} Image credit: Wikipedia Last update: 4-Jan-2015 ...
Optimization and the Robustness of BOX A1R PCR for DNA
... was then spun down and washed with sterile distilled water once. The pellet was then re-suspended in 100 µl of sterile distilled water followed by a heat lysis step (heating for 10 minutes at 95ºC). Seven cell suspension PCR were performed (2A, 2B, 2C, 2D, 2E, 2F and 2G) with 35 PCR cycles per react ...
... was then spun down and washed with sterile distilled water once. The pellet was then re-suspended in 100 µl of sterile distilled water followed by a heat lysis step (heating for 10 minutes at 95ºC). Seven cell suspension PCR were performed (2A, 2B, 2C, 2D, 2E, 2F and 2G) with 35 PCR cycles per react ...
Design-O-Saur - Beyond Benign
... Decode provided information to build a genetically unique dinosaur. Understand that this is a model that could be used to look at human genetics Understand gene sequences and the results of these sequences Materials: Clay – model magic works very well Student sheets Time needed: 1 x 45-60 ...
... Decode provided information to build a genetically unique dinosaur. Understand that this is a model that could be used to look at human genetics Understand gene sequences and the results of these sequences Materials: Clay – model magic works very well Student sheets Time needed: 1 x 45-60 ...
CHUKRASIA TABULARIS OXIDATIVE STRESS Research Article
... extract of different concentrations was mixed with 2.5 ml of phosphate buffer (200mM, pH 6.6) and 2.5 ml of 1% potassium ferricyanide. The mixture was incubated at 50°C for 20 minutes. A volume of 2.5 ml of 10% TCA was then added to the mixture and centrifuged at 3000 rpm for 10 minutes. 2.5 ml of s ...
... extract of different concentrations was mixed with 2.5 ml of phosphate buffer (200mM, pH 6.6) and 2.5 ml of 1% potassium ferricyanide. The mixture was incubated at 50°C for 20 minutes. A volume of 2.5 ml of 10% TCA was then added to the mixture and centrifuged at 3000 rpm for 10 minutes. 2.5 ml of s ...
Mutagenesis Point mutations Deletions Insertions Types of
... and synthesized for processes ranging from biofuel production to pharmaceutical manufacture. Bacteria and plants often carry out syntheses that far exceed what can be readily achieved by the best organic chemists. The genes that control desirable pathways can be chemically synthesized, placed in art ...
... and synthesized for processes ranging from biofuel production to pharmaceutical manufacture. Bacteria and plants often carry out syntheses that far exceed what can be readily achieved by the best organic chemists. The genes that control desirable pathways can be chemically synthesized, placed in art ...
Crystal Structure of Paired Domain -...
... DNA-binding proteins. Among other fields, transcription factors play central roles in the field of development biology --- regulating cell development, differentiation, and cell growth, by binding to specific DNA sites and thereafter activating or inhibiting gene ...
... DNA-binding proteins. Among other fields, transcription factors play central roles in the field of development biology --- regulating cell development, differentiation, and cell growth, by binding to specific DNA sites and thereafter activating or inhibiting gene ...
Formosa haliotis sp. nov., a brown-alga
... Isolates LMG 28520T and LMG 28521 were obtained from the gut of the abalone Haliotis gigantea collected at an abalone hatchery (Owase, Mie, Japan) in July 2009. Isolates LMG 28522 and LMG 28523 were obtained from the same abalone species and the same location in August 2009. These four isolates were ...
... Isolates LMG 28520T and LMG 28521 were obtained from the gut of the abalone Haliotis gigantea collected at an abalone hatchery (Owase, Mie, Japan) in July 2009. Isolates LMG 28522 and LMG 28523 were obtained from the same abalone species and the same location in August 2009. These four isolates were ...
Reclassification of Koreibacter algae as a later heterotypic synonym
... recharacterization under direct comparison in order to clarify their taxonomic position. PvuII RiboPrint patterns and quantitative ratios of cellular fatty acids revealed strain-specific differences between P. marina DSM 21750T (5CTT-37T) and K. algae DSM 22126T (5DSW-2T). The percentage of DNA–DNA ...
... recharacterization under direct comparison in order to clarify their taxonomic position. PvuII RiboPrint patterns and quantitative ratios of cellular fatty acids revealed strain-specific differences between P. marina DSM 21750T (5CTT-37T) and K. algae DSM 22126T (5DSW-2T). The percentage of DNA–DNA ...
Gel immobilization of acrylamide-modified single
... of ssDNA templates immobilized on streptavidin-coated beads. A recently reported third method to prepare ssDNA template involves immobilizing DNA targets, PCR-amplified by primers modified with different functional groups, onto an activated solid surface [19]. This method is effective in fluorescenc ...
... of ssDNA templates immobilized on streptavidin-coated beads. A recently reported third method to prepare ssDNA template involves immobilizing DNA targets, PCR-amplified by primers modified with different functional groups, onto an activated solid surface [19]. This method is effective in fluorescenc ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... Present address: Code 6030, Laboratory for the Structure of Matter, Naval Research Laboratory, Washington DC 20375-5000, USA ...
... Present address: Code 6030, Laboratory for the Structure of Matter, Naval Research Laboratory, Washington DC 20375-5000, USA ...
Non-homologous end-joining partners in a helical dance: structural
... mediated by the β6–β7 loop inserting into the hydrophobic pocket created by the helix–turn–helix structure and β7 from the neighbouring homodimer head domain. This is very similar to the binding model described here between XLF and XRCC4. The interaction region between XLF-(1–233) and XRCC4-(1–164) ...
... mediated by the β6–β7 loop inserting into the hydrophobic pocket created by the helix–turn–helix structure and β7 from the neighbouring homodimer head domain. This is very similar to the binding model described here between XLF and XRCC4. The interaction region between XLF-(1–233) and XRCC4-(1–164) ...
The Role of DNA-PKcs and Artemis in Opening Viral DNA
... DNA hairpin structures in the classical nonhomologous endjoining (NHEJ) repair pathway (26). The Rag proteins also have some roles in opening hairpin-coding ends in V(D)J recombination (13). Although our understanding of the metabolism of DNA hairpin structures at cellular DNA DSBs has progressed, l ...
... DNA hairpin structures in the classical nonhomologous endjoining (NHEJ) repair pathway (26). The Rag proteins also have some roles in opening hairpin-coding ends in V(D)J recombination (13). Although our understanding of the metabolism of DNA hairpin structures at cellular DNA DSBs has progressed, l ...
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
... have one trait in common: they employ nanoscale probes to examine the structural or electronic signatures of individual DNA bases. That is, they rely on physical differences between the bases. This is a major departure from existing sequencing paradigms which rely on chemical techniques and physical ...
... have one trait in common: they employ nanoscale probes to examine the structural or electronic signatures of individual DNA bases. That is, they rely on physical differences between the bases. This is a major departure from existing sequencing paradigms which rely on chemical techniques and physical ...
History of Discoveries in Molecular Biology
... like a Honey-comb, but that the pores of it were not regular. . . . these pores, or cells, . . . were indeed the first microscopical pores I ever saw, and perhaps, that were ever seen, for I had not met with any Writer or Person, that had made any mention of them before this. . . BTC 563 ...
... like a Honey-comb, but that the pores of it were not regular. . . . these pores, or cells, . . . were indeed the first microscopical pores I ever saw, and perhaps, that were ever seen, for I had not met with any Writer or Person, that had made any mention of them before this. . . BTC 563 ...
Hormesis - Illinois Institute of Technology
... neighboring cells More recent studies (Human & Experimental Toxicology (2004) 23: 59ff) show these effects can be observed in whole animals too Mechanisms thought to involve migration of small molecules from one cell to another through gap junctions ...
... neighboring cells More recent studies (Human & Experimental Toxicology (2004) 23: 59ff) show these effects can be observed in whole animals too Mechanisms thought to involve migration of small molecules from one cell to another through gap junctions ...
quantitation of male and female dna in mixed biological samples
... determining chromosomes. Normal males receive an X chromosome from their mother and a Y chromosome from their father and therefore males carry the XY genotype. Normal females receive an X chromosome from their mother and the other X chromosome from their father and are genotypically XX (Butler, 2005 ...
... determining chromosomes. Normal males receive an X chromosome from their mother and a Y chromosome from their father and therefore males carry the XY genotype. Normal females receive an X chromosome from their mother and the other X chromosome from their father and are genotypically XX (Butler, 2005 ...
dna model project - Jessamine County Schools
... Make a fully colored Model of DNA. Your model may be out of any materials that you choose as long as they are not perishable and are sturdy. Please make sure that your model includes at least 16 nucleotides. Each structure should look different from the others, and should clearly show which 2 other ...
... Make a fully colored Model of DNA. Your model may be out of any materials that you choose as long as they are not perishable and are sturdy. Please make sure that your model includes at least 16 nucleotides. Each structure should look different from the others, and should clearly show which 2 other ...
80A Statistical evaluation in forensic DNA typing
... linkage equilibrium — a state in which multilocus genotype proportions are the same in successive generations in a population; where there is statistical independence between alleles at different loci and where the genotype at one locus does not influence the probability of a genotype at another mat ...
... linkage equilibrium — a state in which multilocus genotype proportions are the same in successive generations in a population; where there is statistical independence between alleles at different loci and where the genotype at one locus does not influence the probability of a genotype at another mat ...
microencapsula tion of dna within cross
... homogenized in the chitosan solution for approximately 20 minutes to obtain a uniform suspension. 5% (w/v) carbonyl iron powder (GAF) was then added to the suspension. The organic phase consisted of 50 .ml sunflower oil (Sun Oueen) with 2% (v/v) Span 85 (Atkemix, Orantford, Ont.) as the emulsifier. ...
... homogenized in the chitosan solution for approximately 20 minutes to obtain a uniform suspension. 5% (w/v) carbonyl iron powder (GAF) was then added to the suspension. The organic phase consisted of 50 .ml sunflower oil (Sun Oueen) with 2% (v/v) Span 85 (Atkemix, Orantford, Ont.) as the emulsifier. ...
DNA nanotechnology
DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. Researchers in the field have created static structures such as two- and three-dimensional crystal lattices, nanotubes, polyhedra, and arbitrary shapes, as well as functional devices such as molecular machines and DNA computers. The field is beginning to be used as a tool to solve basic science problems in structural biology and biophysics, including applications in crystallography and spectroscopy for protein structure determination. Potential applications in molecular scale electronics and nanomedicine are also being investigated.The conceptual foundation for DNA nanotechnology was first laid out by Nadrian Seeman in the early 1980s, and the field began to attract widespread interest in the mid-2000s. This use of nucleic acids is enabled by their strict base pairing rules, which cause only portions of strands with complementary base sequences to bind together to form strong, rigid double helix structures. This allows for the rational design of base sequences that will selectively assemble to form complex target structures with precisely controlled nanoscale features. A number of assembly methods are used to make these structures, including tile-based structures that assemble from smaller structures, folding structures using the DNA origami method, and dynamically reconfigurable structures using strand displacement techniques. While the field's name specifically references DNA, the same principles have been used with other types of nucleic acids as well, leading to the occasional use of the alternative name nucleic acid nanotechnology.