How Cations Can Assist DNase I in DNA Binding and Hydrolysis
... degradation [20] and its two disulfide bridges from reduction. Equilibrium dialysis and various spectroscopic studies have led to the conclusion that bpDNase I contains two strong and several weak cation-binding sites [21,22]. More precise information on the number and the location of divalent catio ...
... degradation [20] and its two disulfide bridges from reduction. Equilibrium dialysis and various spectroscopic studies have led to the conclusion that bpDNase I contains two strong and several weak cation-binding sites [21,22]. More precise information on the number and the location of divalent catio ...
Neandertal DNA Sequences and the Origin of Modern Humans
... from 0.4 g of the bone. When the primers L16,209 and H16,271 were used in an amplification from this extract and the product cloned (Figure 2, extract B), ten clones carried the eight differences from the reference sequence observed in the amplifications from the first extract, as well as two change ...
... from 0.4 g of the bone. When the primers L16,209 and H16,271 were used in an amplification from this extract and the product cloned (Figure 2, extract B), ten clones carried the eight differences from the reference sequence observed in the amplifications from the first extract, as well as two change ...
Motifs that discriminate functions in genomic regions
... A previous study from this laboratory identified 63 DNA segments occupied by GATA1 in mouse erythroid cells (10). That study employed chromatin immunoprecipitation (ChIP) by anti-estrogen receptor (anti-ER) antibody to enrich for DNA segments occupied by the hybrid GATA1-ER protein in the G1E-ER4 ce ...
... A previous study from this laboratory identified 63 DNA segments occupied by GATA1 in mouse erythroid cells (10). That study employed chromatin immunoprecipitation (ChIP) by anti-estrogen receptor (anti-ER) antibody to enrich for DNA segments occupied by the hybrid GATA1-ER protein in the G1E-ER4 ce ...
Regulating the Environmental Release of Genetically Engineered
... nology is bringing about a revolution in industrial processes, agriculture, and health care. The scientific novelty of modifying life forms is raising complex and equally novel legal problems. In order to comprehend the profound effect recombinant DNA technology will have on the future of man and to ...
... nology is bringing about a revolution in industrial processes, agriculture, and health care. The scientific novelty of modifying life forms is raising complex and equally novel legal problems. In order to comprehend the profound effect recombinant DNA technology will have on the future of man and to ...
Cloning and characterization of dispersed repetitive DNA derived
... A sex chromosome specific library of R. acetosa was obtained after cloning the DOP-PCR product derived from sex chromosome DNA into the pGEMT-Easy cloning vector. To screen for DNA sequences in the sex chromosomes, the plasmid DNA from approximately 200 randomly selected clones was restricted with E ...
... A sex chromosome specific library of R. acetosa was obtained after cloning the DOP-PCR product derived from sex chromosome DNA into the pGEMT-Easy cloning vector. To screen for DNA sequences in the sex chromosomes, the plasmid DNA from approximately 200 randomly selected clones was restricted with E ...
(p50) 2 complexed to a high
... G8, G22, and G23 form the expanded major groove. The bases at the major groove facing the stack along one side are G18, G19, U20, U21, G22, G23, and G8. The bases that stack on the other side of the major groove are A11, C12, U13, and G14. Remarkably, these 11 bases were absolutely conserved in all ...
... G8, G22, and G23 form the expanded major groove. The bases at the major groove facing the stack along one side are G18, G19, U20, U21, G22, G23, and G8. The bases that stack on the other side of the major groove are A11, C12, U13, and G14. Remarkably, these 11 bases were absolutely conserved in all ...
The orientation bias of Chi sequences is a general tendency of G
... fractions than the averages, many other octamers have even higher fractions ( Fig. 3c). The orientation fractions of the three hexamers CTGGTG, TGGTGG and GCTGGT, which are composites of the Chi sequence, were also above the average (Fig. 3a). As for B. subtilis, the distributions that showed an obv ...
... fractions than the averages, many other octamers have even higher fractions ( Fig. 3c). The orientation fractions of the three hexamers CTGGTG, TGGTGG and GCTGGT, which are composites of the Chi sequence, were also above the average (Fig. 3a). As for B. subtilis, the distributions that showed an obv ...
Peleg O., Brunak S., Trifonov E.N., Nevo E. and Bolshoy A.
... Use of thermodynamic calculations as a method for RNA structure predictions is, to say the least, controversial. In an early study, Trifonov and Bolshoi23 tried to overcome this problem by using multiple alignments for predicting canonical base matching in 5S rRNA. Using this genomic method, they pr ...
... Use of thermodynamic calculations as a method for RNA structure predictions is, to say the least, controversial. In an early study, Trifonov and Bolshoi23 tried to overcome this problem by using multiple alignments for predicting canonical base matching in 5S rRNA. Using this genomic method, they pr ...
Strategies for the in vitro Evolution of Protein
... occurs freely between any two. Furthermore, equal amounts of the initial DNA sequences are recombined. Consider the random recombination of three parent sequences, each of which contains a single mutation. Any given mutation will be incorporated into a progeny sequence with a probability of 1/3; the ...
... occurs freely between any two. Furthermore, equal amounts of the initial DNA sequences are recombined. Consider the random recombination of three parent sequences, each of which contains a single mutation. Any given mutation will be incorporated into a progeny sequence with a probability of 1/3; the ...
Crystal Structure of the Archaeal Heat Shock Regulator from
... High stability of the dimeric structure can be expected as more than one third of the amino acids from each subunit are involved in forming a large dimeric interface area (ca 4200 Å2). In addition to hydrophobic interactions, 18 hydrogen bonds as well as two salt-bridges are also formed at the inter ...
... High stability of the dimeric structure can be expected as more than one third of the amino acids from each subunit are involved in forming a large dimeric interface area (ca 4200 Å2). In addition to hydrophobic interactions, 18 hydrogen bonds as well as two salt-bridges are also formed at the inter ...
Current Topics LNA (Locked Nucleic Acid): High
... (PS) and/or phosphodiester linkages (8, 9). LNA resembles natural nucleic acids with respect to Watson-Crick base pairing. LNAs as fully modified oligomers or as mix-mers containing, for example, LNA and DNA or LNA and RNA nucleotides induce very high thermal stability of duplexes toward complementa ...
... (PS) and/or phosphodiester linkages (8, 9). LNA resembles natural nucleic acids with respect to Watson-Crick base pairing. LNAs as fully modified oligomers or as mix-mers containing, for example, LNA and DNA or LNA and RNA nucleotides induce very high thermal stability of duplexes toward complementa ...
Principles and challenges of genome
... As noted above, 5meC is not readily distinguished from unmethylated cytosine by hybridization-based methods and, as DNA methyltransferases are not present during PCR or in biological cloning systems, DNA methylation information is erased during amplification. some investigators have suggested that i ...
... As noted above, 5meC is not readily distinguished from unmethylated cytosine by hybridization-based methods and, as DNA methyltransferases are not present during PCR or in biological cloning systems, DNA methylation information is erased during amplification. some investigators have suggested that i ...
A TOPRIM Domain in the Crystal Structure of the Catalytic Core of
... metal-binding site of another. This arrangement positions the guanidium group of the arginine residue such that it mimics the metal observed in the TOPRIM domain of topoisomerase VI (not shown). Our attempts to soak nucleotides into this crystal form have been unsuccessful, almost certainly because ...
... metal-binding site of another. This arrangement positions the guanidium group of the arginine residue such that it mimics the metal observed in the TOPRIM domain of topoisomerase VI (not shown). Our attempts to soak nucleotides into this crystal form have been unsuccessful, almost certainly because ...
Replication of Nuclear DNA
... On this basis, it appears that the budding yeast ars core sequence may also be part of plant origins, despite the absence of this sequence in S. pombe origins (as discussed above). However, to date, there has been no direct demonstration of this. Further, since no other plant nuclear DNA replication ...
... On this basis, it appears that the budding yeast ars core sequence may also be part of plant origins, despite the absence of this sequence in S. pombe origins (as discussed above). However, to date, there has been no direct demonstration of this. Further, since no other plant nuclear DNA replication ...
Reconstruction of the original mycoflora in pelleted feed by PCR
... GELCOMPAR II program. The results show that the similarities of DNA band profiles from both feed types (ground vs. pellet) were > 85% up to 97.6%. The within-group variability of ground feed was as high as the group-to-group variability between ground feed and pelleted feed. All intense DNA bands f ...
... GELCOMPAR II program. The results show that the similarities of DNA band profiles from both feed types (ground vs. pellet) were > 85% up to 97.6%. The within-group variability of ground feed was as high as the group-to-group variability between ground feed and pelleted feed. All intense DNA bands f ...
Binding of Hoechst with nucleic acids using fluorescence spectroscopy
... chains. Therefore, the easy penetration of Hoechst into these flexible sites (not into the rigid double helix) in such samples could occur. While binding to cellular DNA, the Hoechst dye can block the DNA replication during cell division. Consequently, Hoechst at low concentrations could be applied, ...
... chains. Therefore, the easy penetration of Hoechst into these flexible sites (not into the rigid double helix) in such samples could occur. While binding to cellular DNA, the Hoechst dye can block the DNA replication during cell division. Consequently, Hoechst at low concentrations could be applied, ...
SHV-type Extended-spectrum ß-lactamase(ESBL) are
... they were confirmed as ESBL producers by the double disc method. In general the MICs for ceftazidime and cefotaxime were heterogeneous. Five of these clinical isolates represent outbreaks or endemic clones at each hospital, some of them reported previously: K806-4, K910-5, K1332-2, K1335-2, K1509-6 a ...
... they were confirmed as ESBL producers by the double disc method. In general the MICs for ceftazidime and cefotaxime were heterogeneous. Five of these clinical isolates represent outbreaks or endemic clones at each hospital, some of them reported previously: K806-4, K910-5, K1332-2, K1335-2, K1509-6 a ...
Categorical spectral analysis of periodicity in
... known genomes. This approach also detects reassortment events, such as those that led to the 2009 pandemic H1N1 virus. INTRODUCTION Genomic nucleotide sequences contain functional and structural information at multiple levels. Even though consensus sequence motifs resulting from local alignments hav ...
... known genomes. This approach also detects reassortment events, such as those that led to the 2009 pandemic H1N1 virus. INTRODUCTION Genomic nucleotide sequences contain functional and structural information at multiple levels. Even though consensus sequence motifs resulting from local alignments hav ...
Detection of Agrobacterium vitis by polymerase chain reaction in
... has rapidly become a basic diagnostic and identification protocol in plant pathology as well (HENSON and FRENCH 1993, LOUWS et al. 1999). Early studies to identify Agrobacterium with PCR used pure bacterial cultures to determine the suitability of primers which were usually designed on ...
... has rapidly become a basic diagnostic and identification protocol in plant pathology as well (HENSON and FRENCH 1993, LOUWS et al. 1999). Early studies to identify Agrobacterium with PCR used pure bacterial cultures to determine the suitability of primers which were usually designed on ...
Identification of a cis-Element That Determines Autonomous DNA
... template in an in vitro DNA replication system using HeLa cell extracts as a source of replication proteins (11). (Previously, we have shown that in this in vitro replication system, initiation is site-specific and maps to the same site as in vivo (8, 11, 19).) To eliminate unreplicated DNA, the rec ...
... template in an in vitro DNA replication system using HeLa cell extracts as a source of replication proteins (11). (Previously, we have shown that in this in vitro replication system, initiation is site-specific and maps to the same site as in vivo (8, 11, 19).) To eliminate unreplicated DNA, the rec ...
Higher order structural effects stabilizing the
... amount of experimental and theoretical studies devoted to characterize energetically and geometrically each of these interactions, with the general idea that they can be considered as building blocks contributing to the overall structure and stability of the nucleic acids. Among theoretical methods, ...
... amount of experimental and theoretical studies devoted to characterize energetically and geometrically each of these interactions, with the general idea that they can be considered as building blocks contributing to the overall structure and stability of the nucleic acids. Among theoretical methods, ...
Frazier, Tamara: The Use of Computational Methods to Describe and Establish Utility of a DNA Sequence for Purposes of Patenting
... composition of matter if they are “made by man”—i.e. created in the lab or isolated from nature. In general, matter in its naturally occurring state cannot be patented, but isolated and purified “products of nature” are eligible for patent protection. Thus, it is now clear that “a DNA sequence itse ...
... composition of matter if they are “made by man”—i.e. created in the lab or isolated from nature. In general, matter in its naturally occurring state cannot be patented, but isolated and purified “products of nature” are eligible for patent protection. Thus, it is now clear that “a DNA sequence itse ...
Rosalind Franklin: Unsung Hero of the DNA Revolution
... actual knowledge of the structure of DNA,6 though they had elementary knowledge of heredity from such scientists as Gregor Mendel.7 The race to discover the structure of DNA was run by many scientists.8 The most notable were Linus Pauling, an American chemist working at Cal Tech, James Watson, an Am ...
... actual knowledge of the structure of DNA,6 though they had elementary knowledge of heredity from such scientists as Gregor Mendel.7 The race to discover the structure of DNA was run by many scientists.8 The most notable were Linus Pauling, an American chemist working at Cal Tech, James Watson, an Am ...
The Preservation and Persistence of Human DNA in Soil during
... the impact of human cadaveric decomposition on the grave soil environment; however, despite such advances, the fate of important biological correlates in grave soil, including human DNA, have remained relatively understudied. This study redresses the current lack of knowledge regarding the preservat ...
... the impact of human cadaveric decomposition on the grave soil environment; however, despite such advances, the fate of important biological correlates in grave soil, including human DNA, have remained relatively understudied. This study redresses the current lack of knowledge regarding the preservat ...
PicoMaxx High Fidelity PCR System
... The PicoMaxx high fidelity PCR system,* is a blend of cloned Taq and Pfu DNA polymerases,** and Agilent’s ArchaeMaxx polymerase-enhancing factor. Together with a specially optimized buffer, this enzyme blend provides the highest success rate of any PCR enzyme, even higher than other Taq-based blends ...
... The PicoMaxx high fidelity PCR system,* is a blend of cloned Taq and Pfu DNA polymerases,** and Agilent’s ArchaeMaxx polymerase-enhancing factor. Together with a specially optimized buffer, this enzyme blend provides the highest success rate of any PCR enzyme, even higher than other Taq-based blends ...
DNA nanotechnology
DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. Researchers in the field have created static structures such as two- and three-dimensional crystal lattices, nanotubes, polyhedra, and arbitrary shapes, as well as functional devices such as molecular machines and DNA computers. The field is beginning to be used as a tool to solve basic science problems in structural biology and biophysics, including applications in crystallography and spectroscopy for protein structure determination. Potential applications in molecular scale electronics and nanomedicine are also being investigated.The conceptual foundation for DNA nanotechnology was first laid out by Nadrian Seeman in the early 1980s, and the field began to attract widespread interest in the mid-2000s. This use of nucleic acids is enabled by their strict base pairing rules, which cause only portions of strands with complementary base sequences to bind together to form strong, rigid double helix structures. This allows for the rational design of base sequences that will selectively assemble to form complex target structures with precisely controlled nanoscale features. A number of assembly methods are used to make these structures, including tile-based structures that assemble from smaller structures, folding structures using the DNA origami method, and dynamically reconfigurable structures using strand displacement techniques. While the field's name specifically references DNA, the same principles have been used with other types of nucleic acids as well, leading to the occasional use of the alternative name nucleic acid nanotechnology.