Detection of DNA molecules by SERS spectroscopy with silvered
... 1 Introduction Detection of DNA molecules today is widely used in many areas of human life such as medical diagnostics, gene therapy, forensic science, etc. [1, 2]. For many years, the polymerase chain reaction and fluorescent spectroscopy have been the most popular methods used in practice to study ...
... 1 Introduction Detection of DNA molecules today is widely used in many areas of human life such as medical diagnostics, gene therapy, forensic science, etc. [1, 2]. For many years, the polymerase chain reaction and fluorescent spectroscopy have been the most popular methods used in practice to study ...
DNA helicase activity in Werner`s syndrome gene
... prediction was made by comparing the sequences and by searching for a homology in a database, no biochemical evidence has been shown before to substantiate that the products of these genes are indeed functional DNA helicases. In general, DNA helicases bind one of the strands of duplex DNA and unwind ...
... prediction was made by comparing the sequences and by searching for a homology in a database, no biochemical evidence has been shown before to substantiate that the products of these genes are indeed functional DNA helicases. In general, DNA helicases bind one of the strands of duplex DNA and unwind ...
Protein–Nucleic Acid Interactions
... segment follows the surface curvature of the packaging protein, and here the bending of the DNA is associated with periodic changes in the geometry of the base steps. Finally, it should be noted that a DNA-binding protein seldom amounts to a rigid surface upon which the DNA moulds its shape: in the ...
... segment follows the surface curvature of the packaging protein, and here the bending of the DNA is associated with periodic changes in the geometry of the base steps. Finally, it should be noted that a DNA-binding protein seldom amounts to a rigid surface upon which the DNA moulds its shape: in the ...
DNA -‐ Compsci 201
... The code below finds all occurrences of a restriction enzyme like “gaattc” and splices in a new strand of DNA, represented by parameter splicee to create a recombinant strand. The strand ...
... The code below finds all occurrences of a restriction enzyme like “gaattc” and splices in a new strand of DNA, represented by parameter splicee to create a recombinant strand. The strand ...
Lactobacillus kimchii sp. nov., a new species from kimchi
... bacteria are known to have the potential to inhibit the growth of micro-organisms, especially pathogenic and spoilage bacteria. The antimicrobial activity of lactic acid bacteria is known to be due to organic acids, hydrogen peroxide, diacetyl and bacteriocins (Dahiya & Speck, 1968 ; Jay, 1982 ; Kla ...
... bacteria are known to have the potential to inhibit the growth of micro-organisms, especially pathogenic and spoilage bacteria. The antimicrobial activity of lactic acid bacteria is known to be due to organic acids, hydrogen peroxide, diacetyl and bacteriocins (Dahiya & Speck, 1968 ; Jay, 1982 ; Kla ...
Chapter 12: From DNA to Protein: Genotype to Phenotype
... • Proteins destined for the ER, Golgi apparatus, lysosomes, and outside the cell complete their synthesis on the ER surface. • They enter the ER by the interaction of a ...
... • Proteins destined for the ER, Golgi apparatus, lysosomes, and outside the cell complete their synthesis on the ER surface. • They enter the ER by the interaction of a ...
A novel assay for examining the molecular
... Nucleic synthesized DNA could be isolated on the paramagnetic, streptavidin beads (e.g. Fig. 2B, lanes 1 and 2). There was a significant enrichment of high molecular weight products and replication intermediates during isolation (Fig. 2B, lane 2). Although the basis of this enrichment has not been d ...
... Nucleic synthesized DNA could be isolated on the paramagnetic, streptavidin beads (e.g. Fig. 2B, lanes 1 and 2). There was a significant enrichment of high molecular weight products and replication intermediates during isolation (Fig. 2B, lane 2). Although the basis of this enrichment has not been d ...
Quantitative real-time PCR assay for rapid detection of
... Quantitative real-time PCR (qRT-PCR) based assays have advantages of speed, accuracy and sensitivity over other detection techniques (Gachon et al. 2004, Schaad and Frederick 2002, Schena et al. 2004, Wong et al. 2005). In addition the qRT-PCR method has been employed to a wide range of fungal patho ...
... Quantitative real-time PCR (qRT-PCR) based assays have advantages of speed, accuracy and sensitivity over other detection techniques (Gachon et al. 2004, Schaad and Frederick 2002, Schena et al. 2004, Wong et al. 2005). In addition the qRT-PCR method has been employed to a wide range of fungal patho ...
Part 1: Have Your DNA and Eat It Too!
... 1. Create a 9 base DNA sequence and record it in your notebook. You will use this sequence to create a DNA strand that is nine bases long. You must use at least one of each base. You can choose any order with a few exceptions. You are NOT allowed to choose the following 3 bases in order: ATC, ATT, A ...
... 1. Create a 9 base DNA sequence and record it in your notebook. You will use this sequence to create a DNA strand that is nine bases long. You must use at least one of each base. You can choose any order with a few exceptions. You are NOT allowed to choose the following 3 bases in order: ATC, ATT, A ...
Sequence-specific RNA Photocleavage by Single
... for isoalloxazine derivatives which was obtained from in vitro selection18–20. As shown in Fig. 1A, they found the riboflavin could specifically recognize the G•U wobble in RNA duplex and induce the oxidative cleavage reaction through photosensitization. The Cleavage occurred independently of the re ...
... for isoalloxazine derivatives which was obtained from in vitro selection18–20. As shown in Fig. 1A, they found the riboflavin could specifically recognize the G•U wobble in RNA duplex and induce the oxidative cleavage reaction through photosensitization. The Cleavage occurred independently of the re ...
Diversity of DNA methyltransferases that recognize asymmetric
... palindromic DNA sequences and add a methyl group to the target base (either adenine or cytosine) on both strands. However, there are a number of MTases that recognize asymmetric target sequences and differ in their subunit organization. In a bacterial cell, after each round of replication, the subst ...
... palindromic DNA sequences and add a methyl group to the target base (either adenine or cytosine) on both strands. However, there are a number of MTases that recognize asymmetric target sequences and differ in their subunit organization. In a bacterial cell, after each round of replication, the subst ...
HLA-B27 real-time PCR using TaqMan
... The aim of our study was to adapt our conventional allele specific HLA-B27 protocol to a fluorogenic real time (RT-PCR) method. The allele specific PCR detects the presence of the B*27 genotype by amplifying a region between primer sets that recognise only B*27 specific sequences. The PCR reaction a ...
... The aim of our study was to adapt our conventional allele specific HLA-B27 protocol to a fluorogenic real time (RT-PCR) method. The allele specific PCR detects the presence of the B*27 genotype by amplifying a region between primer sets that recognise only B*27 specific sequences. The PCR reaction a ...
Text S2.
... Tables S2-S3. A fragment of divL encoding residues 523-769 (used in crystallization) was amplified by PCR from the template cosmid 2G1 [1] using primers WSCp8 and WSCp11. These primers introduced NdeI and EcoR1 restriction sites at the 5’- and 3’-ends. This PCR fragment was digested with NdeI and Ec ...
... Tables S2-S3. A fragment of divL encoding residues 523-769 (used in crystallization) was amplified by PCR from the template cosmid 2G1 [1] using primers WSCp8 and WSCp11. These primers introduced NdeI and EcoR1 restriction sites at the 5’- and 3’-ends. This PCR fragment was digested with NdeI and Ec ...
The Structures of DNA and RNA
... he discovery that DNA is the prime genetic molecule, carrying all the hereditary information within chromosomes, immediately focused attention on its structure. It was hoped that knowledge of the structure would reveal how DNA carries the genetic messages that are replicated when chromosomes divide ...
... he discovery that DNA is the prime genetic molecule, carrying all the hereditary information within chromosomes, immediately focused attention on its structure. It was hoped that knowledge of the structure would reveal how DNA carries the genetic messages that are replicated when chromosomes divide ...
DNA Profile 911 - Bertino Forensics
... of accident victims, plane crashes, paternity and forensic cases. This lab provides an example of how DNA technology is being applied to solve real life situations. The 911 lab is an introductory lab that provides students with a background of STRs and the graphic print-out of a STR profile known as ...
... of accident victims, plane crashes, paternity and forensic cases. This lab provides an example of how DNA technology is being applied to solve real life situations. The 911 lab is an introductory lab that provides students with a background of STRs and the graphic print-out of a STR profile known as ...
Distinct mechanisms of DNA repair in mycobacteria - MCBL
... into a murine macrophage cell line (Kurthkoti et al., 2008; our unpublished results). Taken together, these observations suggest NER to be a useful drug target, and the gene knockouts in NER pathway as a means to generate attenuated strains. Recently, a chemical inhibitor 2-(5-amino-1, 3, 4-thiadiaz ...
... into a murine macrophage cell line (Kurthkoti et al., 2008; our unpublished results). Taken together, these observations suggest NER to be a useful drug target, and the gene knockouts in NER pathway as a means to generate attenuated strains. Recently, a chemical inhibitor 2-(5-amino-1, 3, 4-thiadiaz ...
Noninvasive prenatal diagnosis of fetal chromosomal aneuploidies
... particularly valuable because methylation-sensitive restriction enzymes that cut hypomethylated sequences but leave hypermethylated sequences intact can be used for the selective destruction of the maternal sequences in maternal plasma (22 ). The extension of such a restriction enzyme– based approac ...
... particularly valuable because methylation-sensitive restriction enzymes that cut hypomethylated sequences but leave hypermethylated sequences intact can be used for the selective destruction of the maternal sequences in maternal plasma (22 ). The extension of such a restriction enzyme– based approac ...
Infectivity analysis of two variable DNA B components of Mungbean
... (pGA1⋅9B22) (Jacob et al 2003) and KA27 (pGA1⋅5B27) DNA B components of MYMV-Vig, each containing two origins of replication as direct repeats, were constructed in a modified version of the binary vector pGA472 (An et al 1985). The partial dimer clone pGA1⋅5B27 was constructed in this study by cloni ...
... (pGA1⋅9B22) (Jacob et al 2003) and KA27 (pGA1⋅5B27) DNA B components of MYMV-Vig, each containing two origins of replication as direct repeats, were constructed in a modified version of the binary vector pGA472 (An et al 1985). The partial dimer clone pGA1⋅5B27 was constructed in this study by cloni ...
6 Core Chapter 6
... the same? • All the cells in your body contain the same DNA. But do all the cells make the same ...
... the same? • All the cells in your body contain the same DNA. But do all the cells make the same ...
Bacteria Screening PCR Kit
... pH8.0) then suspend in a 200 μl of Chelex® solution. 2) Heat at 99℃ for a period of 5 minutes then quickly cool by placing on ice for 1 minute or more. 3) Centrifuge at 12,000 rpm (approx. 13,000 X g ) for 1 minute. 4) Use the supernatant as the DNA Sample Solution for PCR. [ Option 2 ] (Use o ...
... pH8.0) then suspend in a 200 μl of Chelex® solution. 2) Heat at 99℃ for a period of 5 minutes then quickly cool by placing on ice for 1 minute or more. 3) Centrifuge at 12,000 rpm (approx. 13,000 X g ) for 1 minute. 4) Use the supernatant as the DNA Sample Solution for PCR. [ Option 2 ] (Use o ...
Evidence for Variable Selective Pressures at a
... For permissions, please e-mail: [email protected] ...
... For permissions, please e-mail: [email protected] ...
The energetic basis of the DNA double helix: a
... and widely accepted, that the enthalpy of DNA duplex dissociation/association does not depend on temperature (10–13). On the other hand, the enthalpy of DNA dissociation at elevated temperatures, determined by DSC, was found to be in conflict with the enthalpy of association of the complementary str ...
... and widely accepted, that the enthalpy of DNA duplex dissociation/association does not depend on temperature (10–13). On the other hand, the enthalpy of DNA dissociation at elevated temperatures, determined by DSC, was found to be in conflict with the enthalpy of association of the complementary str ...
Lesson Overview
... Comparing RNA and DNA Each nucleotide in both DNA and RNA is made up of a 5-carbon sugar, a phosphate group, and a nitrogenous base. There are three important differences between RNA and DNA: (1) The sugar in RNA is ribose instead of deoxyribose. (2) RNA is generally single-stranded and not double-s ...
... Comparing RNA and DNA Each nucleotide in both DNA and RNA is made up of a 5-carbon sugar, a phosphate group, and a nitrogenous base. There are three important differences between RNA and DNA: (1) The sugar in RNA is ribose instead of deoxyribose. (2) RNA is generally single-stranded and not double-s ...
Lesson Overview
... Comparing RNA and DNA Each nucleotide in both DNA and RNA is made up of a 5-carbon sugar, a phosphate group, and a nitrogenous base. There are three important differences between RNA and DNA: (1) The sugar in RNA is ribose instead of deoxyribose. (2) RNA is generally single-stranded and not double-s ...
... Comparing RNA and DNA Each nucleotide in both DNA and RNA is made up of a 5-carbon sugar, a phosphate group, and a nitrogenous base. There are three important differences between RNA and DNA: (1) The sugar in RNA is ribose instead of deoxyribose. (2) RNA is generally single-stranded and not double-s ...
Chapter 5 PowerPoint
... • A DNA molecule has two polynucleotides spiraling around an imaginary axis, forming a double helix • In the DNA double helix, the two backbones run in opposite 5 → 3 directions from each other, an arrangement referred to as antiparallel • One DNA molecule includes many genes • The nitrogenous bas ...
... • A DNA molecule has two polynucleotides spiraling around an imaginary axis, forming a double helix • In the DNA double helix, the two backbones run in opposite 5 → 3 directions from each other, an arrangement referred to as antiparallel • One DNA molecule includes many genes • The nitrogenous bas ...
DNA nanotechnology
DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. Researchers in the field have created static structures such as two- and three-dimensional crystal lattices, nanotubes, polyhedra, and arbitrary shapes, as well as functional devices such as molecular machines and DNA computers. The field is beginning to be used as a tool to solve basic science problems in structural biology and biophysics, including applications in crystallography and spectroscopy for protein structure determination. Potential applications in molecular scale electronics and nanomedicine are also being investigated.The conceptual foundation for DNA nanotechnology was first laid out by Nadrian Seeman in the early 1980s, and the field began to attract widespread interest in the mid-2000s. This use of nucleic acids is enabled by their strict base pairing rules, which cause only portions of strands with complementary base sequences to bind together to form strong, rigid double helix structures. This allows for the rational design of base sequences that will selectively assemble to form complex target structures with precisely controlled nanoscale features. A number of assembly methods are used to make these structures, including tile-based structures that assemble from smaller structures, folding structures using the DNA origami method, and dynamically reconfigurable structures using strand displacement techniques. While the field's name specifically references DNA, the same principles have been used with other types of nucleic acids as well, leading to the occasional use of the alternative name nucleic acid nanotechnology.