A new heavy lanthanide-dependent DNAzyme
... RESULTS AND DISCUSSION In vitro selection Free Ln3+ and some of their complexes can cleave RNA at high metal concentrations (1,17). We hope to use DNA as a scaffold to more efficiently use Ln3+ ions so that the same reaction takes place at much lower Ln3+ concentrations and at a designated position. ...
... RESULTS AND DISCUSSION In vitro selection Free Ln3+ and some of their complexes can cleave RNA at high metal concentrations (1,17). We hope to use DNA as a scaffold to more efficiently use Ln3+ ions so that the same reaction takes place at much lower Ln3+ concentrations and at a designated position. ...
Polymorphisms and Genomic Organization of Repetitive
... The centromere of each chromosome (except for Y and deleted or rearranged abnormal chromosomes) includes tandemly arrayed units of the 170-bp monomer repeat arranged in head-to-tail orientation, and many thousands of units occur in each single array that may be megabases long. Different chromosomes ...
... The centromere of each chromosome (except for Y and deleted or rearranged abnormal chromosomes) includes tandemly arrayed units of the 170-bp monomer repeat arranged in head-to-tail orientation, and many thousands of units occur in each single array that may be megabases long. Different chromosomes ...
3-4 DNA F11 - De Anza College
... Copyright © 2007 Pearson Education Inc., publishing as Pearson Benjamin Cummings ...
... Copyright © 2007 Pearson Education Inc., publishing as Pearson Benjamin Cummings ...
1-6 DNA Sp12
... The daughter strand is compared to the parent DNA to check for mistakes Proofreading is not perfect – mutations are still possible, although rare ...
... The daughter strand is compared to the parent DNA to check for mistakes Proofreading is not perfect – mutations are still possible, although rare ...
Preventing transcriptional gene silencing by active DNA demethylation
... specific ribonuclease III, Dicer [16]. In several systems, these small interfering RNAs (siRNAs) have been shown to interact with other proteins such as Argonaute to form an RNA-induced silencing complex (RISC) and target homologous mRNAs for degradation [17,18]. Several well-known epigenetic phenome ...
... specific ribonuclease III, Dicer [16]. In several systems, these small interfering RNAs (siRNAs) have been shown to interact with other proteins such as Argonaute to form an RNA-induced silencing complex (RISC) and target homologous mRNAs for degradation [17,18]. Several well-known epigenetic phenome ...
Genetic Information in the Age of DNA Sequencing
... Crick managed to model the double helical structure of DNA in 1953. Crick has always described Edwin Schrödinger’s What Is Life? as a decisive reading for his move to biology.9 Schrödinger was one of the founders of quantum theory, and in this book, published in 1944, he attempted to apply quantum ...
... Crick managed to model the double helical structure of DNA in 1953. Crick has always described Edwin Schrödinger’s What Is Life? as a decisive reading for his move to biology.9 Schrödinger was one of the founders of quantum theory, and in this book, published in 1944, he attempted to apply quantum ...
Insights into Protein–DNA Interactions through Structure
... investigations have been carried out from the protein point of view (protein-centric), and the present network approach aims to combine both the protein-centric and the DNA-centric points of view. Part of the study involves the development of methodology to investigate protein–DNA graphs/networks wi ...
... investigations have been carried out from the protein point of view (protein-centric), and the present network approach aims to combine both the protein-centric and the DNA-centric points of view. Part of the study involves the development of methodology to investigate protein–DNA graphs/networks wi ...
Intrinsic sequence specificity of the Cas1 integrase directs
... activity for a variety of substrates differing in the nature of the 5’-flap or duplex arm released by the ...
... activity for a variety of substrates differing in the nature of the 5’-flap or duplex arm released by the ...
Atomistic model of DNA: Phonons and base-pair opening
... A fully atomistic model of B-DNA using the CHARMM 共chemistry at Harvard molecular mechanics兲 force field is presented. Molecular dynamics simulations were used to prepare an equilibrium structure. The Hessian of interatomic forces obtained from CHARMM for the equilibrium structure was used as input ...
... A fully atomistic model of B-DNA using the CHARMM 共chemistry at Harvard molecular mechanics兲 force field is presented. Molecular dynamics simulations were used to prepare an equilibrium structure. The Hessian of interatomic forces obtained from CHARMM for the equilibrium structure was used as input ...
Coffee, B, Muralidharan, K, Highsmith Jr., WE, Lapunzina, P and Warren, ST: Molecular diagnosis of Beckwith-Wiedemann Syndrome using quantitative methylation-sensitive polymerase chain reaction; Genetics in Medicine, Vol 8 - No.10 (2006).
... In this method, genomic DNA is treated with sodium bisulfite, which deaminates unmethylated cytosines to uracil. Methylated cytosines are resistant to this deamination and after sodium bisulfite treatment will remain as cytosines. After desulfonation and purification the DNA is used as a template in ...
... In this method, genomic DNA is treated with sodium bisulfite, which deaminates unmethylated cytosines to uracil. Methylated cytosines are resistant to this deamination and after sodium bisulfite treatment will remain as cytosines. After desulfonation and purification the DNA is used as a template in ...
RNA vs. DNA
... Question for Structure- $400 • These two parts of the nucleotide make up the sides to the structure. ...
... Question for Structure- $400 • These two parts of the nucleotide make up the sides to the structure. ...
Genome-wide DNA methylation analysis
... of DNA involved in a variety of biological processes, including embryogenesis and development, silencing of transposable elements, regulation of gene transcription and tumorigenesis and progression. The methylation pattern of DNA is highly variable among cells ...
... of DNA involved in a variety of biological processes, including embryogenesis and development, silencing of transposable elements, regulation of gene transcription and tumorigenesis and progression. The methylation pattern of DNA is highly variable among cells ...
jeopardy practice
... Question for Structure- $400 • These two parts of the nucleotide make up the sides to the structure. ...
... Question for Structure- $400 • These two parts of the nucleotide make up the sides to the structure. ...
Synthesis and Evaluation of Guanidino Phthalocyanines for G
... repeats can fold into triple helix-containing structures called H-DNA. Upon H-DNA formation, the DNA double helix within one-half of the H-palindrome denatures into two single strands, and one of these complementary strands folds back to form a DNA triplex with the nondenatured half of the H-palindr ...
... repeats can fold into triple helix-containing structures called H-DNA. Upon H-DNA formation, the DNA double helix within one-half of the H-palindrome denatures into two single strands, and one of these complementary strands folds back to form a DNA triplex with the nondenatured half of the H-palindr ...
Education®
... 2. Antiparallel – a term describing the two side rails of the ladder-like structure of a double stranded DNA molecule. The ladder is formed when two strands of DNA lie parallel to each other and are hydrogen-bonded together through the nitrogen-containing bases that form the “rungs.” Rep ...
... 2. Antiparallel – a term describing the two side rails of the ladder-like structure of a double stranded DNA molecule. The ladder is formed when two strands of DNA lie parallel to each other and are hydrogen-bonded together through the nitrogen-containing bases that form the “rungs.” Rep ...
Essential knowledge 3.A.1 - local.brookings.k12.sd.us
... Essential knowledge 3.C.2: Biological systems have multiple processes that increase genetic variation. c. Sexual reproduction in eukaryotes involving gamete formation, including crossing-over during meiosis and the random assortment of chromosomes during meiosis, and fertilization serve to increase ...
... Essential knowledge 3.C.2: Biological systems have multiple processes that increase genetic variation. c. Sexual reproduction in eukaryotes involving gamete formation, including crossing-over during meiosis and the random assortment of chromosomes during meiosis, and fertilization serve to increase ...
Translation
... • tRNA carries over the proper amino acid – tRNA anticodon matches with the mRNA codon – Prevents delivery of wrong amino acid • One by one, amino acids are linked together • Translation ends when a “stop” codon is reached • What just happened?: A ribosome made a protein ...
... • tRNA carries over the proper amino acid – tRNA anticodon matches with the mRNA codon – Prevents delivery of wrong amino acid • One by one, amino acids are linked together • Translation ends when a “stop” codon is reached • What just happened?: A ribosome made a protein ...
Transcription blockage by stable H-DNA analogs in
... and another as a donor of the third strand (Figure 1A). During H-DNA formation, the donor stretch dissociates into two single strands. One strand winds back down the major groove of the acceptor stretch forming the triplex via Hoogsteen-type base pairing, while the other remains single-stranded. Bot ...
... and another as a donor of the third strand (Figure 1A). During H-DNA formation, the donor stretch dissociates into two single strands. One strand winds back down the major groove of the acceptor stretch forming the triplex via Hoogsteen-type base pairing, while the other remains single-stranded. Bot ...
Revisiting Plus-Strand DNA Synthesis in Retroviruses and Long
... positioned for RNase H-mediated cleavage at the biologically-relevant PPT/U3 junction, this structure nonetheless revealed anomalous hydrogen bonding between base pairs -9 and -15 (defining position -1 as the first g:C base pair upstream of the PPT/U3 junction (note that throughout the manuscript, R ...
... positioned for RNase H-mediated cleavage at the biologically-relevant PPT/U3 junction, this structure nonetheless revealed anomalous hydrogen bonding between base pairs -9 and -15 (defining position -1 as the first g:C base pair upstream of the PPT/U3 junction (note that throughout the manuscript, R ...
DNA sequence selectivity of guanine–N7 alkylation by nitrogen
... Nucleic Acids Research amine piperidine converts these modified base sites into strand breaks (18). If the DNA is labeled only at one end of one strand, and is of known sequence, the lengths of the labeled fragments produced after alkylation and subsequent alkaline piperidine treatment indicate the ...
... Nucleic Acids Research amine piperidine converts these modified base sites into strand breaks (18). If the DNA is labeled only at one end of one strand, and is of known sequence, the lengths of the labeled fragments produced after alkylation and subsequent alkaline piperidine treatment indicate the ...
The cleavage site of the restriction endonuclease
... GACN and GTCN. Inspection of their data, however, shows that N is predominantly C. We suggest that the very slight ...
... GACN and GTCN. Inspection of their data, however, shows that N is predominantly C. We suggest that the very slight ...
16 System and a 10X Primer Pair Mix Stored in TE
... dNTP and 1.6mg/ml BSA) and added MgCl2 to the reactions separately to a final concentration of 1mM, 1.25mM, 1.5mM, 1.75mM or 2mM. For both primer formulations, the reactions produced similar results (Figure 1). The best balance between loci was achieved with 1.5mM MgCl2. Increasing MgCl2 concentrati ...
... dNTP and 1.6mg/ml BSA) and added MgCl2 to the reactions separately to a final concentration of 1mM, 1.25mM, 1.5mM, 1.75mM or 2mM. For both primer formulations, the reactions produced similar results (Figure 1). The best balance between loci was achieved with 1.5mM MgCl2. Increasing MgCl2 concentrati ...
DNA metabarcoding multiplexing and validation of
... 2012). This feature, summed to the ability of using uniquely tagged primers for pooling together several PCR products in a single sequencing run (Valentini et al. 2009b; Coissac 2012), makes the processing of large sample numbers feasible and cost effective. This strategy has an obvious benefit for ...
... 2012). This feature, summed to the ability of using uniquely tagged primers for pooling together several PCR products in a single sequencing run (Valentini et al. 2009b; Coissac 2012), makes the processing of large sample numbers feasible and cost effective. This strategy has an obvious benefit for ...
More Taxonomy, Not DNA Barcoding
... that there exist “DNA species” that are impossible to tell apart from known species except through sequence comparisons, many new species identified by barcoding would not be morphologically distinguishable. The resulting, much larger number of “species” would be a number that means very little. Wha ...
... that there exist “DNA species” that are impossible to tell apart from known species except through sequence comparisons, many new species identified by barcoding would not be morphologically distinguishable. The resulting, much larger number of “species” would be a number that means very little. Wha ...
DNA nanotechnology
DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. Researchers in the field have created static structures such as two- and three-dimensional crystal lattices, nanotubes, polyhedra, and arbitrary shapes, as well as functional devices such as molecular machines and DNA computers. The field is beginning to be used as a tool to solve basic science problems in structural biology and biophysics, including applications in crystallography and spectroscopy for protein structure determination. Potential applications in molecular scale electronics and nanomedicine are also being investigated.The conceptual foundation for DNA nanotechnology was first laid out by Nadrian Seeman in the early 1980s, and the field began to attract widespread interest in the mid-2000s. This use of nucleic acids is enabled by their strict base pairing rules, which cause only portions of strands with complementary base sequences to bind together to form strong, rigid double helix structures. This allows for the rational design of base sequences that will selectively assemble to form complex target structures with precisely controlled nanoscale features. A number of assembly methods are used to make these structures, including tile-based structures that assemble from smaller structures, folding structures using the DNA origami method, and dynamically reconfigurable structures using strand displacement techniques. While the field's name specifically references DNA, the same principles have been used with other types of nucleic acids as well, leading to the occasional use of the alternative name nucleic acid nanotechnology.