Inhibition of RNA Synthesis by Anthracycline Analogs
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
bacterial plasmids - Acta Medica Medianae
... Plasmids contain genes essential for plasmid function and their preservation in a host cell (the beginning and the control of replication). Some of them possess genes which control plasmid stability. There is a common opinion that plasmids are unnecessary for a growth of bacterial population and the ...
... Plasmids contain genes essential for plasmid function and their preservation in a host cell (the beginning and the control of replication). Some of them possess genes which control plasmid stability. There is a common opinion that plasmids are unnecessary for a growth of bacterial population and the ...
Part III: Laboratory – Electrophoresis
... The laboratory is organized by part. Each part has notes for the instructor (when necessary), preparation instructions, and the experimental protocol for the lab. A separate Results and Discussion is also provided with some additional suggested analysis. The lab is set-up to have students work in pa ...
... The laboratory is organized by part. Each part has notes for the instructor (when necessary), preparation instructions, and the experimental protocol for the lab. A separate Results and Discussion is also provided with some additional suggested analysis. The lab is set-up to have students work in pa ...
Use of a novel cassette to label phenotypically a cryptic plasmid of
... replication A restriction map of pPOD2000 is shown in Fig. 2, using coordinates originating at the unique SalI site. Previous attempts to insert a selectable HindIII fragment into the HindIII sites of pPOD2000 gave insertions at all sites except that at coordinate 2.2 kb, suggesting that this site l ...
... replication A restriction map of pPOD2000 is shown in Fig. 2, using coordinates originating at the unique SalI site. Previous attempts to insert a selectable HindIII fragment into the HindIII sites of pPOD2000 gave insertions at all sites except that at coordinate 2.2 kb, suggesting that this site l ...
Natiiona Str An l D ate nua 20 NA gy l R 15/ A Da Boa epo /16 tab rd
... billion’. This is to ensure that the courts continue to understand the likelihood that the DNA found could match to a different individual than the one on trial. Certain cases might be reported with a more precise probability; this is assessed on a case-by-case basis. ...
... billion’. This is to ensure that the courts continue to understand the likelihood that the DNA found could match to a different individual than the one on trial. Certain cases might be reported with a more precise probability; this is assessed on a case-by-case basis. ...
Review Synthetic oligonucleotides as RNA mimetics
... dichroism (CD) spectra of phosphoramidate DNA duplexes were consistent with formation of an RNA-like A-form conformation [17]. This change in the helix geometry compared with DNA is likely due to N-type sugar puckering of 3%-aminonucleosides. These findings were confirmed by the X-ray analysis of cr ...
... dichroism (CD) spectra of phosphoramidate DNA duplexes were consistent with formation of an RNA-like A-form conformation [17]. This change in the helix geometry compared with DNA is likely due to N-type sugar puckering of 3%-aminonucleosides. These findings were confirmed by the X-ray analysis of cr ...
Binding of Hoechst with nucleic acids using fluorescence spectroscopy
... of Hoechst here was in ~ 1.5 - 2 times less than in the case of HP1 or t-RNA. It means that Hoechst interacts with the double helix not such strong as with single chains or hairpin structures. The life-time of Hoechst at binding with DNA was increased from 0.4 ns to only 3 ns. Furthermore, there was ...
... of Hoechst here was in ~ 1.5 - 2 times less than in the case of HP1 or t-RNA. It means that Hoechst interacts with the double helix not such strong as with single chains or hairpin structures. The life-time of Hoechst at binding with DNA was increased from 0.4 ns to only 3 ns. Furthermore, there was ...
Intelligent Icons: Integrating Lite-Weight Data Mining
... The three files in the example are ASCII text files, each of which contains approximately 16,000 base pairs of mitochondrial DNA. Here we used string edit distance as suggested in [15] to measure the distance between file names, and Euclidean distance to measure the distance between the file icons ( ...
... The three files in the example are ASCII text files, each of which contains approximately 16,000 base pairs of mitochondrial DNA. Here we used string edit distance as suggested in [15] to measure the distance between file names, and Euclidean distance to measure the distance between the file icons ( ...
RecA maintains the integrity of chloroplast DNA molecules in
... nuclear genomes of many organisms, including plants (Lin et al., 2006). Repair and recombination of chloroplast DNA (cpDNA) in Chlamydomonas reinhardtii is suppressed when a dominant-negative version of E. coli RecA is targeted to chloroplasts (Cerutti et al., 1995). The prevalence of chloroplast-ta ...
... nuclear genomes of many organisms, including plants (Lin et al., 2006). Repair and recombination of chloroplast DNA (cpDNA) in Chlamydomonas reinhardtii is suppressed when a dominant-negative version of E. coli RecA is targeted to chloroplasts (Cerutti et al., 1995). The prevalence of chloroplast-ta ...
Week 2. DNA isolation and PCR
... calculated in their pre-laboratory assignment. Some students will not have found the reverse PCR primer because they do not understand that the reverse primer sequence needs to be reversed and complemented before they can find and exact sequence match of the primer in the gene sequence. I often have ...
... calculated in their pre-laboratory assignment. Some students will not have found the reverse PCR primer because they do not understand that the reverse primer sequence needs to be reversed and complemented before they can find and exact sequence match of the primer in the gene sequence. I often have ...
The Large Loop Repair and Mismatch Repair Pathways
... mitotic growth. NER functions to repair bulky DNA lesions, such as thymine dimers and other helix-distorting lesions. During NER the damaged nucleotide is recognized and bound by several NER proteins, and the DNA surrounding the lesion is unwound. The single-stranded DNA containing the lesion is rem ...
... mitotic growth. NER functions to repair bulky DNA lesions, such as thymine dimers and other helix-distorting lesions. During NER the damaged nucleotide is recognized and bound by several NER proteins, and the DNA surrounding the lesion is unwound. The single-stranded DNA containing the lesion is rem ...
light - Microbiology
... population by increasing the number of cells which can transfer the bacterial chromosome. In contrast, under similar conditions the fertility of irradiated Hfr populations falls in proportion to the survivors. Following irradiation, the effect begins to develop after about 30 min. incubation in brot ...
... population by increasing the number of cells which can transfer the bacterial chromosome. In contrast, under similar conditions the fertility of irradiated Hfr populations falls in proportion to the survivors. Following irradiation, the effect begins to develop after about 30 min. incubation in brot ...
Ultraviolet Induction of Chromosome Transfer by
... population by increasing the number of cells which can transfer the bacterial chromosome. In contrast, under similar conditions the fertility of irradiated Hfr populations falls in proportion to the survivors. Following irradiation, the effect begins to develop after about 30 min. incubation in brot ...
... population by increasing the number of cells which can transfer the bacterial chromosome. In contrast, under similar conditions the fertility of irradiated Hfr populations falls in proportion to the survivors. Following irradiation, the effect begins to develop after about 30 min. incubation in brot ...
RHD - Labex
... Wiener and Landsteiner discovered the Rh factor in 1937/1940 The importance of the Rh factor was the better blood finger print for criminal matters M, N, or P factors where known and Rh factor was just an additional one Later it was recognized that the new Rh factor was associated with probl ...
... Wiener and Landsteiner discovered the Rh factor in 1937/1940 The importance of the Rh factor was the better blood finger print for criminal matters M, N, or P factors where known and Rh factor was just an additional one Later it was recognized that the new Rh factor was associated with probl ...
Analysis and nucleotide sequence of an origin of DNA replication in
... complementing a trpE mutation carried a 20-kb insertion and transformed with a 30-fold higher efficiency when compared to the vector. A deletion analysis of the shuttle plasmid indicates that 2.2 kb is necessary for autonomous replication and stable maintenance in A. calcoaceticus. No rearrangements ...
... complementing a trpE mutation carried a 20-kb insertion and transformed with a 30-fold higher efficiency when compared to the vector. A deletion analysis of the shuttle plasmid indicates that 2.2 kb is necessary for autonomous replication and stable maintenance in A. calcoaceticus. No rearrangements ...
Intelligent Icons: Integrating Lite-Weight Data Mining
... These results are something of a mixed bag for us. Although the clustering is objectively correct, the differences detected by Euclidean distance measure are very subtle to the naked eye. For example one must look quite closely to observe that the top right element of the primates bitmap is pink, wh ...
... These results are something of a mixed bag for us. Although the clustering is objectively correct, the differences detected by Euclidean distance measure are very subtle to the naked eye. For example one must look quite closely to observe that the top right element of the primates bitmap is pink, wh ...
Reconstruction of the original mycoflora in pelleted feed by PCR
... quantify fungal DNA in these feed types, PCR-based methods were applied. The DNA bands in SSCP gel were analyzed with the GELCOMPAR II program. The results show that the similarities of DNA band profiles from both feed types (ground vs. pellet) were > 85% up to 97.6%. The within-group variability o ...
... quantify fungal DNA in these feed types, PCR-based methods were applied. The DNA bands in SSCP gel were analyzed with the GELCOMPAR II program. The results show that the similarities of DNA band profiles from both feed types (ground vs. pellet) were > 85% up to 97.6%. The within-group variability o ...
Transparent DNA/RNA Co-extraction Workflow Protocol Suitable for
... Adequate comparisons of DNA and cDNA libraries from complex environments require methods for co-extraction of DNA and RNA due to the inherent heterogeneity of such samples, or risk bias caused by variations in lysis and extraction efficiencies. Still, there are few methods and kits allowing simultan ...
... Adequate comparisons of DNA and cDNA libraries from complex environments require methods for co-extraction of DNA and RNA due to the inherent heterogeneity of such samples, or risk bias caused by variations in lysis and extraction efficiencies. Still, there are few methods and kits allowing simultan ...
What is PCR? - Cobb Learning
... Can’t make it’s own reverse transcriptase (RT)…so…it hijacks another gene ...
... Can’t make it’s own reverse transcriptase (RT)…so…it hijacks another gene ...
Relationships Between RNA Polymerase II Activity and Spt
... yeast genome and many of these exhibit modulation by many factors (Martens et al. 2004, 2005; Hainer et al. 2011; Bird et al. 2006). Furthermore, widespread antisense transcription is being revealed as a mechanism for shaping gene regulation in a number of ways, including transcription over promoter ...
... yeast genome and many of these exhibit modulation by many factors (Martens et al. 2004, 2005; Hainer et al. 2011; Bird et al. 2006). Furthermore, widespread antisense transcription is being revealed as a mechanism for shaping gene regulation in a number of ways, including transcription over promoter ...
Optimization and the Robustness of BOX A1R PCR for DNA
... shaped bands were seen in the gel. The PCR products from reaction 2G, with the same conditions as reactions 2E and 2F, were separated in 3 different 1.5% agarose gels made from Biorad, Amresco or Invitrogen agarose but no banding patterns were observed from these products. However, there were also n ...
... shaped bands were seen in the gel. The PCR products from reaction 2G, with the same conditions as reactions 2E and 2F, were separated in 3 different 1.5% agarose gels made from Biorad, Amresco or Invitrogen agarose but no banding patterns were observed from these products. However, there were also n ...
CYP2B6 NESTED PCR: A GOOD APPROACH FOR PATIENTS ON METHADONE Original Article
... The importance of pharmacogenomic research for determining patient-specific drug susceptibilities and elucidating drug toxicities is increasing progressively. Hence, genotyping pharmacologically relevant SNPs may be a useful forensic tool [15–17]. It has been shown that most of the pharmacologically ...
... The importance of pharmacogenomic research for determining patient-specific drug susceptibilities and elucidating drug toxicities is increasing progressively. Hence, genotyping pharmacologically relevant SNPs may be a useful forensic tool [15–17]. It has been shown that most of the pharmacologically ...
mMESSAGE mMACHINE® Kit User Guide
... mMACHINE® Kits are ideal for the routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection and other applications. The high yields are achieved by optimizing reaction conditions for RNA synthesis in the presence of high nucleotide concentrations. In addition, the ...
... mMACHINE® Kits are ideal for the routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection and other applications. The high yields are achieved by optimizing reaction conditions for RNA synthesis in the presence of high nucleotide concentrations. In addition, the ...
SNP
... Synchronized DNA synthesis – Pyrosequencing Structure specific cleavage -- the Invader assay The 5’ exonuclease activity -- the TaqMan assay ...
... Synchronized DNA synthesis – Pyrosequencing Structure specific cleavage -- the Invader assay The 5’ exonuclease activity -- the TaqMan assay ...
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides
... Two ruthenium cations (Ru1) are symmetrically equivalent, related by the twofold axis running through the centre of the duplex, with a third cation (Ru2) lying on the same axis and being bound at the central step of the duplex (T5A6/T5A6). The ruthenium atoms lie in the minor groove of the duplex, w ...
... Two ruthenium cations (Ru1) are symmetrically equivalent, related by the twofold axis running through the centre of the duplex, with a third cation (Ru2) lying on the same axis and being bound at the central step of the duplex (T5A6/T5A6). The ruthenium atoms lie in the minor groove of the duplex, w ...
DNA polymerase
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.