Distortion of quantitative genomic and expression
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
NUCLEIC ACID ECONOMY IN BACTERIA INFECTED WITH
... 0.1 gin. gelatin, and HC1 to adjust to pH 7.4. Analytical Methods.--Most of the experiments required periodic measures of DNAP~ and mature phage P~. DNA-P 3~ was determined (Schmidt and Thannhauser, 1945) by precipitating a chilled 1 ml. sample of the culture in a conical tube with 9 ml. 0.3 M trich ...
... 0.1 gin. gelatin, and HC1 to adjust to pH 7.4. Analytical Methods.--Most of the experiments required periodic measures of DNAP~ and mature phage P~. DNA-P 3~ was determined (Schmidt and Thannhauser, 1945) by precipitating a chilled 1 ml. sample of the culture in a conical tube with 9 ml. 0.3 M trich ...
Maintenance of genomic integrity by p53: complementary
... state, i.e., in the absence of signals indicating DNA damage or other potentially harmful conditions, so far has not yet been considered at all. In contrast, the general assumption is that a non-activated p53 is a non-functional one (Hupp and Lane, 1994; Vogelstein and Kinzler, 1992). In this review ...
... state, i.e., in the absence of signals indicating DNA damage or other potentially harmful conditions, so far has not yet been considered at all. In contrast, the general assumption is that a non-activated p53 is a non-functional one (Hupp and Lane, 1994; Vogelstein and Kinzler, 1992). In this review ...
Investigating the DNA-Binding Interactions of Small
... and other disorders. In this dissertation, the DNA-binding interactions of small organic molecules were investigated. We developed a highly sensitive methodology to determine the DNA-binding mode of small molecules unambiguously unlike other conventional techniques. Furthermore, we established struc ...
... and other disorders. In this dissertation, the DNA-binding interactions of small organic molecules were investigated. We developed a highly sensitive methodology to determine the DNA-binding mode of small molecules unambiguously unlike other conventional techniques. Furthermore, we established struc ...
SURVEY AND SUMMARY New insights into Hoogsteen base pairs
... which the adenine base was flipped 180◦ to form a unique set of hydrogen bonds (H-bonds) (Figure 1). Two years earlier, Rich et al. had speculated that such a pairing scheme could explain how poly(rU) associates with poly(rA)-poly(rU) duplexes to form RNA triplexes (2). These bps are now referred to ...
... which the adenine base was flipped 180◦ to form a unique set of hydrogen bonds (H-bonds) (Figure 1). Two years earlier, Rich et al. had speculated that such a pairing scheme could explain how poly(rU) associates with poly(rA)-poly(rU) duplexes to form RNA triplexes (2). These bps are now referred to ...
Transposon stability and a role for conjugational transfer in adaptive mutability
... (16–21). Moreover, even lacI33 Lac⫺ cells, products of conjugal transfer to scavenger, frequently had become TetS. About 10% of the late-appearing Lac⫹ revertants that are TetS have lost the transposon DNA, most often precisely, and an equal number show evidence of amplification of the Lac兾Tn geneti ...
... (16–21). Moreover, even lacI33 Lac⫺ cells, products of conjugal transfer to scavenger, frequently had become TetS. About 10% of the late-appearing Lac⫹ revertants that are TetS have lost the transposon DNA, most often precisely, and an equal number show evidence of amplification of the Lac兾Tn geneti ...
Analysis of clones carrying repeated DNA sequences in two YAC
... coordinates of the YAC clones which hybridized to chloroplast DNA with the coordinates of clones which had been mapped previously on to chromosome 4 and the top half of chromosome 5 using RFLP markers as probes (Hwang eta/., 1991; Schmidt etal., unpublished results). One-hundred-and-ninety-five clon ...
... coordinates of the YAC clones which hybridized to chloroplast DNA with the coordinates of clones which had been mapped previously on to chromosome 4 and the top half of chromosome 5 using RFLP markers as probes (Hwang eta/., 1991; Schmidt etal., unpublished results). One-hundred-and-ninety-five clon ...
Structure, stability and function of 5-chlorouracil
... synthesized in the 1950s as potential anti-tumor agents (2,3). The 5-fluorouracil (FU) analog is a well-known anti-cancer drug for treatment of human malignancies (4). 5-Chlorouracil and 5-bromouracil (ClU and BrU, respectively) are associated with inflammation and are considered to be carcinogenic ( ...
... synthesized in the 1950s as potential anti-tumor agents (2,3). The 5-fluorouracil (FU) analog is a well-known anti-cancer drug for treatment of human malignancies (4). 5-Chlorouracil and 5-bromouracil (ClU and BrU, respectively) are associated with inflammation and are considered to be carcinogenic ( ...
DNA Evidence: Probability, Population Genetics, and the Courts
... where, as the court of appeals put it, he "ravished" her and drove away in her car. A policeman issued a traffic citation to Kenneth Cobey, who was driving that car. Celimark Diagnostics performed a "DNA fingerprint analysis" showing "a 'match' between the DNA in Cobey's blood sample and the DNA [ex ...
... where, as the court of appeals put it, he "ravished" her and drove away in her car. A policeman issued a traffic citation to Kenneth Cobey, who was driving that car. Celimark Diagnostics performed a "DNA fingerprint analysis" showing "a 'match' between the DNA in Cobey's blood sample and the DNA [ex ...
Nanopore Unzipping of Individual DNA Hairpin Molecules
... 2003). The response time of the membrane potential to a step in the control voltage was 4 6 1 ms. In each experiment (performed at given conditions set by the voltage or the voltage ramp) we typically collected over 1000 unzipping events. Our software and hardware combination permits highthroughput ...
... 2003). The response time of the membrane potential to a step in the control voltage was 4 6 1 ms. In each experiment (performed at given conditions set by the voltage or the voltage ramp) we typically collected over 1000 unzipping events. Our software and hardware combination permits highthroughput ...
Press release
... based on the use of magnetic particles. These particles can specifically bind nucleic acids such as DNA and thus allow the separation of DNA from other contaminants. sbeadex is based on a two-stage binding mechanism, so that water can be used for the final washing step. This avoids having to dry the ...
... based on the use of magnetic particles. These particles can specifically bind nucleic acids such as DNA and thus allow the separation of DNA from other contaminants. sbeadex is based on a two-stage binding mechanism, so that water can be used for the final washing step. This avoids having to dry the ...
Replication timing as an epigenetic mark
... a few dozen individual gene loci using molecular approaches, with the finding that early replicating genes could be either expressed or silent, while late replicating genes were almost always silent, leading to the hypothesis that early replication is necessary for transcriptional competence but is ...
... a few dozen individual gene loci using molecular approaches, with the finding that early replicating genes could be either expressed or silent, while late replicating genes were almost always silent, leading to the hypothesis that early replication is necessary for transcriptional competence but is ...
microencapsula tion of dna within cross
... homogenized in the chitosan solution for approximately 20 minutes to obtain a uniform suspension. 5% (w/v) carbonyl iron powder (GAF) was then added to the suspension. The organic phase consisted of 50 .ml sunflower oil (Sun Oueen) with 2% (v/v) Span 85 (Atkemix, Orantford, Ont.) as the emulsifier. ...
... homogenized in the chitosan solution for approximately 20 minutes to obtain a uniform suspension. 5% (w/v) carbonyl iron powder (GAF) was then added to the suspension. The organic phase consisted of 50 .ml sunflower oil (Sun Oueen) with 2% (v/v) Span 85 (Atkemix, Orantford, Ont.) as the emulsifier. ...
Structure and function of the GINS complex, a key component of the
... and DDK substrates is key to understanding how replication initiation is regulated. S-CDK substrates are abundant in yeast but two essential proteins, Sld2 and Sld3, have been shown to comprise the minimal set required for replication initiation [21,22]. Phosphorylation of Sld3 by S-CDK on Thr600 an ...
... and DDK substrates is key to understanding how replication initiation is regulated. S-CDK substrates are abundant in yeast but two essential proteins, Sld2 and Sld3, have been shown to comprise the minimal set required for replication initiation [21,22]. Phosphorylation of Sld3 by S-CDK on Thr600 an ...
Assessing the Probative Value of DNA Evidence
... Criminal adjudication in the UK’s legal jurisdictions is strongly wedded to the principle of lay fact-finding by juries and magistrates employing their ordinary common sense reasoning. Notwithstanding the unquestionable merits of lay involvement in criminal trials, it cannot be assumed that jurors o ...
... Criminal adjudication in the UK’s legal jurisdictions is strongly wedded to the principle of lay fact-finding by juries and magistrates employing their ordinary common sense reasoning. Notwithstanding the unquestionable merits of lay involvement in criminal trials, it cannot be assumed that jurors o ...
The Role of DNA-PKcs and Artemis in Opening Viral DNA
... 3,000 ds-vg/dge). Unusual bands that were not present in samples from wild-type mice were observed in PstI (noncutter) DNA digests in DNA-PKcs-deficient mouse livers (shown with arrows). The major unusual bands migrated slightly faster than a 3,049-bp marker, which is 20 bp shorter than full-length ...
... 3,000 ds-vg/dge). Unusual bands that were not present in samples from wild-type mice were observed in PstI (noncutter) DNA digests in DNA-PKcs-deficient mouse livers (shown with arrows). The major unusual bands migrated slightly faster than a 3,049-bp marker, which is 20 bp shorter than full-length ...
Molecular Biology of Transcription and RNA Processing
... Figure 8.1. The structure of uracil is similar to that of thymine, but notice, by comparing the structure of uracil in Figure 8.1 with that of thymine in Figure 7.5, that thymine has a methyl group (CH3) at the 5 carbon of the pyrimidine ring, whereas uracil does not. In all other respects, uracil i ...
... Figure 8.1. The structure of uracil is similar to that of thymine, but notice, by comparing the structure of uracil in Figure 8.1 with that of thymine in Figure 7.5, that thymine has a methyl group (CH3) at the 5 carbon of the pyrimidine ring, whereas uracil does not. In all other respects, uracil i ...
Defining characteristics of Tn5 Transposase non
... domains of the bacteriophage Mu Tnp (MuA) independently bind DNA non-specifically. Filter binding experiments with proteolytic fragments of MuA reveal that the N-terminal 26 amino acids of domain III binds supercoiled DNA nonspecifically (3) and that domain II can interact with linear non-specific D ...
... domains of the bacteriophage Mu Tnp (MuA) independently bind DNA non-specifically. Filter binding experiments with proteolytic fragments of MuA reveal that the N-terminal 26 amino acids of domain III binds supercoiled DNA nonspecifically (3) and that domain II can interact with linear non-specific D ...
CC 1 070-71..8171c ose chapter .. Page70
... classes of molecules with high triplex affinity can be identified. A pyrene–neomycin conjugate has therefore been synthesized by forming an amide linkage between neomycin B and 1-pyrenebutyric acid N-hydroxysuccinimide ester (Fig. 1). We report that pyrene–neomycin is more potent in stabilizing DNA ...
... classes of molecules with high triplex affinity can be identified. A pyrene–neomycin conjugate has therefore been synthesized by forming an amide linkage between neomycin B and 1-pyrenebutyric acid N-hydroxysuccinimide ester (Fig. 1). We report that pyrene–neomycin is more potent in stabilizing DNA ...
quantitation of male and female dna in mixed biological samples
... noncoding regions of autosomal chromosomes and are useful for human identification purposes because they provide the ability to discriminate between samples. When biological fluids are recovered (normally as stains) from a crime scene, DNA can be isolated from nucleated cells present in the fluid. ...
... noncoding regions of autosomal chromosomes and are useful for human identification purposes because they provide the ability to discriminate between samples. When biological fluids are recovered (normally as stains) from a crime scene, DNA can be isolated from nucleated cells present in the fluid. ...
Article Mitochondrial DNA turnover occurs during preimplantation
... Each mitochondrion contains many copies of its own circular genome, which is continuously turned over, being synthesized by a mitochondrial specific DNA polymerase γ (Lestienne, 1987). The exact molecular mechanism controlling the amount of mtDNA per cell is still uncertain (Moraes, 2001; Bogenhagen ...
... Each mitochondrion contains many copies of its own circular genome, which is continuously turned over, being synthesized by a mitochondrial specific DNA polymerase γ (Lestienne, 1987). The exact molecular mechanism controlling the amount of mtDNA per cell is still uncertain (Moraes, 2001; Bogenhagen ...
DNA polymerase
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.