Non-homologous end-joining partners in a helical dance: structural
... stabilizing DNA ligase IV at DSBs in NHEJ DNA DSBs (double-strand breaks) can be caused by ionizing radiation or toxic chemical exposure, but are also present as intermediates in V(D)J recombination and class switch recombination for antigen receptor diversity formation. Unrepaired DSBs lead to chro ...
... stabilizing DNA ligase IV at DSBs in NHEJ DNA DSBs (double-strand breaks) can be caused by ionizing radiation or toxic chemical exposure, but are also present as intermediates in V(D)J recombination and class switch recombination for antigen receptor diversity formation. Unrepaired DSBs lead to chro ...
HIV-1 Reverse Transcriptase-associated
... The reverse transcriptase (RT) of HIV-1 contains two active sites, polymerase and ribonuclease (RNase) H that are both absolutely necessary for viral replication and disease progression. While several drugs currently used in the clinic to treat HIV-1 target the polymerase activity of RT, none curren ...
... The reverse transcriptase (RT) of HIV-1 contains two active sites, polymerase and ribonuclease (RNase) H that are both absolutely necessary for viral replication and disease progression. While several drugs currently used in the clinic to treat HIV-1 target the polymerase activity of RT, none curren ...
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
... genomes can be produced and propagated.-A physical and genetic map of the P22 genome near the prophage attachment site was constructed which shows that the insertion from the R-factor in P22Tc-10 is not a t the attachment site: it is therefore unlikely that P22Tc-10 was formed in an abnormal prophag ...
... genomes can be produced and propagated.-A physical and genetic map of the P22 genome near the prophage attachment site was constructed which shows that the insertion from the R-factor in P22Tc-10 is not a t the attachment site: it is therefore unlikely that P22Tc-10 was formed in an abnormal prophag ...
Getting Started With Gel Electrophoresis
... This gel can then be placed in an electrophoresis chamber, which along with a power supply, creates an electrical field through the gel. When a charged molecule is placed in this electric field, a force will act upon it. Negative charges will be pulled towards the positive side of the electric field ...
... This gel can then be placed in an electrophoresis chamber, which along with a power supply, creates an electrical field through the gel. When a charged molecule is placed in this electric field, a force will act upon it. Negative charges will be pulled towards the positive side of the electric field ...
Downloaded - Laboratoire de Chimie Théorique
... are either potent mutagens or inhibitors. We used structural and topological analyses based on ab initio QM/MM calculations to study human DNA polymerase l (Poll) with different metals in the active site. Our results indicate a slightly longer O3 0 –Pa distance (B3.6 Å) for most inhibitor cations co ...
... are either potent mutagens or inhibitors. We used structural and topological analyses based on ab initio QM/MM calculations to study human DNA polymerase l (Poll) with different metals in the active site. Our results indicate a slightly longer O3 0 –Pa distance (B3.6 Å) for most inhibitor cations co ...
Human RIF1 and protein phosphatase 1 stimulate DNA replication
... in RIF1 overexpressing cells and ascribe this observation to reduced helicase activation, yet overexpression of the RIF1-PP1 interaction mutant does not curb this observation as their hypothesis predicts. The authors show RIF1 overexpression exacerbates the replication defects observed using a DDK i ...
... in RIF1 overexpressing cells and ascribe this observation to reduced helicase activation, yet overexpression of the RIF1-PP1 interaction mutant does not curb this observation as their hypothesis predicts. The authors show RIF1 overexpression exacerbates the replication defects observed using a DDK i ...
Single-stranded heteroduplex intermediates in l Red homologous
... Background: The Red proteins of lambda phage mediate probably the simplest and most efficient homologous recombination reactions yet described. However the mechanism of dsDNA recombination remains undefined. Results: Here we show that the Red proteins can act via full length single stranded intermed ...
... Background: The Red proteins of lambda phage mediate probably the simplest and most efficient homologous recombination reactions yet described. However the mechanism of dsDNA recombination remains undefined. Results: Here we show that the Red proteins can act via full length single stranded intermed ...
Gel immobilization of acrylamide-modified single
... involves tedious procedures. There are two widely used strategies for preparing ssDNA templates. One is directly preparing the templates from double-stranded PCR products with a set of enzymatic reactions that remove excess primers and nucleotides [9]. However, the template/primer complexes formed b ...
... involves tedious procedures. There are two widely used strategies for preparing ssDNA templates. One is directly preparing the templates from double-stranded PCR products with a set of enzymatic reactions that remove excess primers and nucleotides [9]. However, the template/primer complexes formed b ...
Basic 2D gel protocols - Personal Web Site Joel Huberman
... bromide), with 22 wells across the top, each well having a cross section of 5 mm 1-2 mm. Due to focussing at the well/gel boundary, the resolution obtained with the 2 mm well width is as good as with the 1 mm width. The gel may be formed as thick as necessary to accommodate the sample volume, up ...
... bromide), with 22 wells across the top, each well having a cross section of 5 mm 1-2 mm. Due to focussing at the well/gel boundary, the resolution obtained with the 2 mm well width is as good as with the 1 mm width. The gel may be formed as thick as necessary to accommodate the sample volume, up ...
Human DNA Ligase IV and the Ligase IV/XRCC4 Complex: Analysis
... intolerance of nicks containing mismatched base pairs. Although human DNA ligase I (Okazaki fragment processing) and the human DNA ligase III/XRCC1 complex (general DNA repair) have been shown to be relatively intolerant of nicks containing mismatched base pairs, the human DNA ligase IV/XRCC4 comple ...
... intolerance of nicks containing mismatched base pairs. Although human DNA ligase I (Okazaki fragment processing) and the human DNA ligase III/XRCC1 complex (general DNA repair) have been shown to be relatively intolerant of nicks containing mismatched base pairs, the human DNA ligase IV/XRCC4 comple ...
DNA METHODS FOR HLA TYPING A WORKBOOK FOR - ASHI-U
... lower than the example shown above? What would be the melting temperature of the 18 base pair sequence if it was made up of only G-C pairs? Only A-T pairs? ...
... lower than the example shown above? What would be the melting temperature of the 18 base pair sequence if it was made up of only G-C pairs? Only A-T pairs? ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... tree weighting method and the LZ-based compression. Long exact/approximate repeats and complementary palindromes repeats are encoded by the LZ-based algorithm, whereas short subsequences are compressed using CTW. Although it obtains good compression ratio, its execution time is too high for long seq ...
... tree weighting method and the LZ-based compression. Long exact/approximate repeats and complementary palindromes repeats are encoded by the LZ-based algorithm, whereas short subsequences are compressed using CTW. Although it obtains good compression ratio, its execution time is too high for long seq ...
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
... of DNA. Most importantly, these proposals challenge our understanding of, and ability to manipulate and probe, physical processes at the interface between solids, liquids, and biomolecules down to the nanometer scale regime (Di Ventra et al., 2004). Indeed, in order to understand the feasibility, sp ...
... of DNA. Most importantly, these proposals challenge our understanding of, and ability to manipulate and probe, physical processes at the interface between solids, liquids, and biomolecules down to the nanometer scale regime (Di Ventra et al., 2004). Indeed, in order to understand the feasibility, sp ...
Replication timing and transcriptional control: beyond
... assays for replication [29]. There are only two mammalian loci for which both replication-timing switches and origin localization have been studied. At the mouse immunoglobulin IgH locus, new origins are activated in pre-B cells when the promoter region of this locus becomes transcriptionally active ...
... assays for replication [29]. There are only two mammalian loci for which both replication-timing switches and origin localization have been studied. At the mouse immunoglobulin IgH locus, new origins are activated in pre-B cells when the promoter region of this locus becomes transcriptionally active ...
Two Components of the RNA-Directed DNA Methylation Pathway
... single-stranded RNAs produced by Pol IV into double-stranded RNAs [14]. The Dicer-like protein DCL3 acts as an RNase to cleave the double-stranded RNAs into 24-nucleotide (nt) small interfering RNAs (siRNAs), which are loaded onto AGO4 and form an AGO4-siRNA complex required for DNA methylation [19– ...
... single-stranded RNAs produced by Pol IV into double-stranded RNAs [14]. The Dicer-like protein DCL3 acts as an RNase to cleave the double-stranded RNAs into 24-nucleotide (nt) small interfering RNAs (siRNAs), which are loaded onto AGO4 and form an AGO4-siRNA complex required for DNA methylation [19– ...
Gene Detection Systems Catalog
... your name from the dropdown USER name field. If you are a 'new' user, select NEW and enter your name, email address and telephone number. This information will be stored and your name will appear the next time you login. Order Changes and Cancellation All catalog product orders are routinely shipped ...
... your name from the dropdown USER name field. If you are a 'new' user, select NEW and enter your name, email address and telephone number. This information will be stored and your name will appear the next time you login. Order Changes and Cancellation All catalog product orders are routinely shipped ...
as a PDF - CiteSeerX
... not altered by pms1, msh2, and msh3 mismatch repair mutations. Similar to our previous observations for replication slippage-mediated deletions, there were >5-bp identical runs at the recombination breakpoints. We propose that the dramatic increase in recombination results from enhancement of the ef ...
... not altered by pms1, msh2, and msh3 mismatch repair mutations. Similar to our previous observations for replication slippage-mediated deletions, there were >5-bp identical runs at the recombination breakpoints. We propose that the dramatic increase in recombination results from enhancement of the ef ...
Phylogenomics of type II DNA topoisomerases
... the A subunit of TopoVI is homologous to the eukaryotic Spo11 protein.(4) This relationship led to an important breakthrough in the understanding of the molecular basis of meiosis with the identification of Spo11. This enzyme is responsible for the formation of the chromosomal double-strand breaks t ...
... the A subunit of TopoVI is homologous to the eukaryotic Spo11 protein.(4) This relationship led to an important breakthrough in the understanding of the molecular basis of meiosis with the identification of Spo11. This enzyme is responsible for the formation of the chromosomal double-strand breaks t ...
Multiplex in-vitro Detection using SERS
... changes which occurs upon interaction with the chemotherapeutic agent cisplatin. They were able to observe greater spectral changes on interaction with cisplatin than with its analogue transplatin, which possesses a lower affinity for DNA, therefore it was ...
... changes which occurs upon interaction with the chemotherapeutic agent cisplatin. They were able to observe greater spectral changes on interaction with cisplatin than with its analogue transplatin, which possesses a lower affinity for DNA, therefore it was ...
pyrimidine
... Uses short primer that attaches to the 3’ end of the ssDNA, after which a specially engineered DNA polymerase Each vial includes one dideoxyXTP and 3 ordinary dXTPs; the dideoxyXTP will be incorporated but will halt synthesis because the 3’ position is blocked. See figs. 11.3 & 11.4 for how these ar ...
... Uses short primer that attaches to the 3’ end of the ssDNA, after which a specially engineered DNA polymerase Each vial includes one dideoxyXTP and 3 ordinary dXTPs; the dideoxyXTP will be incorporated but will halt synthesis because the 3’ position is blocked. See figs. 11.3 & 11.4 for how these ar ...
Mutagenesis Point mutations Deletions Insertions Types of
... previously been synthesized (4). Since then, two additional in vitro recombination methods have been developed by JCVI to join and clone DNA molecules larger than 300 kb in a single step (2-4). The simplest of these methods is Gibson Assembly, a one-step isothermal approach that utilizes the same th ...
... previously been synthesized (4). Since then, two additional in vitro recombination methods have been developed by JCVI to join and clone DNA molecules larger than 300 kb in a single step (2-4). The simplest of these methods is Gibson Assembly, a one-step isothermal approach that utilizes the same th ...
Molecular Biology and Applied Genetics
... Molecular genetics, or molecular biology, is the study of the biochemical mechanisms of inheritance. It is the study of the biochemical nature of the genetic material and its control of phenotype. It is the study of the connection between genotype and phenotype. The connection is a chemical one. Con ...
... Molecular genetics, or molecular biology, is the study of the biochemical mechanisms of inheritance. It is the study of the biochemical nature of the genetic material and its control of phenotype. It is the study of the connection between genotype and phenotype. The connection is a chemical one. Con ...
Bacteriophage A cloning system for the construction of
... libraries. The wild-type A genome contains five BamHI, five EcoRI, and seven Hindu restriction sites that have all been removed from the genome of AORF8. Sites for these endonucleases are present within the multiple cloning site of AORF8. We report a method for preparing cDNAs that can be cloned in ...
... libraries. The wild-type A genome contains five BamHI, five EcoRI, and seven Hindu restriction sites that have all been removed from the genome of AORF8. Sites for these endonucleases are present within the multiple cloning site of AORF8. We report a method for preparing cDNAs that can be cloned in ...
DNA polymerase
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.