High-Throughput NGS Library Preparation
... In general, adapter ligation efficiency remains robust over a relatively wide range of adapter:insert molar ratios, making it unnecessary to adjust adapter concentrations for individual samples. It is nevertheless important to select an adapter concentration that is suitable for the range of DNA inp ...
... In general, adapter ligation efficiency remains robust over a relatively wide range of adapter:insert molar ratios, making it unnecessary to adjust adapter concentrations for individual samples. It is nevertheless important to select an adapter concentration that is suitable for the range of DNA inp ...
Physiological characterization of natural transformation in
... all the HindIII sites that are present in these plasmids. ...
... all the HindIII sites that are present in these plasmids. ...
The orientation bias of Chi sequences is a general tendency of G
... even higher fractions ( Fig. 3c). The orientation fractions of the three hexamers CTGGTG, TGGTGG and GCTGGT, which are composites of the Chi sequence, were also above the average (Fig. 3a). As for B. subtilis, the distributions that showed an obvious G content bias were similar to those of E. coli ( ...
... even higher fractions ( Fig. 3c). The orientation fractions of the three hexamers CTGGTG, TGGTGG and GCTGGT, which are composites of the Chi sequence, were also above the average (Fig. 3a). As for B. subtilis, the distributions that showed an obvious G content bias were similar to those of E. coli ( ...
lac
... mutated, will grow on lactose. • However they make β-galactosidase all of the time. These mutants that have lost the ability to control gene expression are called constitutive mutants. They are also recessive. ...
... mutated, will grow on lactose. • However they make β-galactosidase all of the time. These mutants that have lost the ability to control gene expression are called constitutive mutants. They are also recessive. ...
Analysis of Guanine Oxidation Products in Double
... 2.2.1. Isolation and Identification of Oligomers Containing Ghox In Figure 5, four major peaks were detected at 19.6 and 20.0 min, with the peaks at 20.5 and 21.2 min having shoulder peaks. Two products (19.6 and 20.0 min) from Figure 5 were isolated and analyzed using electrospray ionization-mass s ...
... 2.2.1. Isolation and Identification of Oligomers Containing Ghox In Figure 5, four major peaks were detected at 19.6 and 20.0 min, with the peaks at 20.5 and 21.2 min having shoulder peaks. Two products (19.6 and 20.0 min) from Figure 5 were isolated and analyzed using electrospray ionization-mass s ...
Protein Function
... is the bacterial single-stranded DNA–binding protein (SSB). SSB binds and protects single-stranded DNA as it is transiently created during DNA replication and repair. The importance of this protein is illustrated by a simple observation: if SSB function is lost, the cell dies. The structure of the ...
... is the bacterial single-stranded DNA–binding protein (SSB). SSB binds and protects single-stranded DNA as it is transiently created during DNA replication and repair. The importance of this protein is illustrated by a simple observation: if SSB function is lost, the cell dies. The structure of the ...
CORPORATE RESPONSIBILITY AT DNA CORPORATE
... thought to responsibility from the company point of view, or how wide-spread its scope is, until I participated in the training last spring. While I may not be the most ecoconscious person out there, it was great to hear about DNA’s commitment as a company to reduce greenhouse gas emissions and use ...
... thought to responsibility from the company point of view, or how wide-spread its scope is, until I participated in the training last spring. While I may not be the most ecoconscious person out there, it was great to hear about DNA’s commitment as a company to reduce greenhouse gas emissions and use ...
SNP Analysis of the PTC Gene Using PCR
... (Figure 2). This fact was discovered in 1931 in a series of events that involved impressive scientific curiosity and questionable laboratory safety. A chemist named Arthur Fox was mixing a powdered chemical when he accidentally let a bit of the powder blow into the air. A nearby colleague exclaimed h ...
... (Figure 2). This fact was discovered in 1931 in a series of events that involved impressive scientific curiosity and questionable laboratory safety. A chemist named Arthur Fox was mixing a powdered chemical when he accidentally let a bit of the powder blow into the air. A nearby colleague exclaimed h ...
Lecture 1
... • Prophase 1: Each chromosome duplicates and remains closely associated. These are called sister chromatids. Crossing-over can occur during the latter part of this stage. • Metaphase 1: Homologous chromosomes align at the equatorial ...
... • Prophase 1: Each chromosome duplicates and remains closely associated. These are called sister chromatids. Crossing-over can occur during the latter part of this stage. • Metaphase 1: Homologous chromosomes align at the equatorial ...
RECOMBINEERING: A POWERFUL NEW TOOL FOR MOUSE
... site, the exonuclease activity of RecBCD is attenuated, but because it maintains its helicase activity, it acts on the linear dsDNA to produce a long, single-stranded DNA (ssDNA) tail with a 3′ end. This ssDNA tail is postulated to be the substrate for DNA strand transfer that is promoted by RecA (s ...
... site, the exonuclease activity of RecBCD is attenuated, but because it maintains its helicase activity, it acts on the linear dsDNA to produce a long, single-stranded DNA (ssDNA) tail with a 3′ end. This ssDNA tail is postulated to be the substrate for DNA strand transfer that is promoted by RecA (s ...
Cloning of genes from genomic DNA: Part 3
... by PCR were simply a blunt-ended fragment, we could just cut the plasmid with a restriction enzyme that leaves blunt ends as well and ligate the two together (blunt-ended cloning works, but is less efficient than cloning sticky ends). When PCR was first used to clone fragments, many people tried thi ...
... by PCR were simply a blunt-ended fragment, we could just cut the plasmid with a restriction enzyme that leaves blunt ends as well and ligate the two together (blunt-ended cloning works, but is less efficient than cloning sticky ends). When PCR was first used to clone fragments, many people tried thi ...
Title Heterochromatin Blocks Constituting the Entire
... Figure 3. Tandem repeat structure revealed through dot matrix analysis. Sequences were compared with dot matrices using the criterion that a 70% match should exist over a window of 10 nucleotides. The scales are shown in units of nucleotides. (A) Entire region of the 5.1-kb OwlRep sequence compared ...
... Figure 3. Tandem repeat structure revealed through dot matrix analysis. Sequences were compared with dot matrices using the criterion that a 70% match should exist over a window of 10 nucleotides. The scales are shown in units of nucleotides. (A) Entire region of the 5.1-kb OwlRep sequence compared ...
Using an Alu Insertion Polymorphism to Study Human
... recognition site for the endonuclease AluI in its middle. Although Alu is sometimes called a “jumping gene,” it is not properly a gene, because it does not produce a protein product. Alu transposons are found only in primate genomes and have accumulated in large numbers since primates diverged from ...
... recognition site for the endonuclease AluI in its middle. Although Alu is sometimes called a “jumping gene,” it is not properly a gene, because it does not produce a protein product. Alu transposons are found only in primate genomes and have accumulated in large numbers since primates diverged from ...
DNA damage in round spermatids of mice with a targeted disruption
... DNA damage in round spermatids assessed under phase contrast for the presence of testicular spermatozoa or round spermatids, in cases where very few spermatozoa were found. If testicular spermatozoa were found, these were assessed for concentration, motility and morphology using World Health Organi ...
... DNA damage in round spermatids assessed under phase contrast for the presence of testicular spermatozoa or round spermatids, in cases where very few spermatozoa were found. If testicular spermatozoa were found, these were assessed for concentration, motility and morphology using World Health Organi ...
Protein A gene expression is regulated by DNA supercoiling which
... As the expression of the protein A gene is dramatically repressed by high salt concentrations (1 M NaCl) and as high osmolarity alters the degree of plasmid DNA supercoiling, we looked at the effect of changing the degree of DNA supercoiling on protein A gene expression. We first determined the conc ...
... As the expression of the protein A gene is dramatically repressed by high salt concentrations (1 M NaCl) and as high osmolarity alters the degree of plasmid DNA supercoiling, we looked at the effect of changing the degree of DNA supercoiling on protein A gene expression. We first determined the conc ...
Mutual Interactions of the Phosphate Groups in Locally Deformed
... (Borah et al. 1985; Nishimura et al. 1986; Sarma et al. 1986). It is also worth of being noticed that at 0.7 mol/l NaCl the backbones of both B- and A-DNA tend to overwind, presumably to counteract base stacking tendencies to unwind the double helices (Šponer and Kypr 1989). At high-salt, even the i ...
... (Borah et al. 1985; Nishimura et al. 1986; Sarma et al. 1986). It is also worth of being noticed that at 0.7 mol/l NaCl the backbones of both B- and A-DNA tend to overwind, presumably to counteract base stacking tendencies to unwind the double helices (Šponer and Kypr 1989). At high-salt, even the i ...
Evolutionary Origin and Adaptive Function of Meiosis
... 2004). In eukaryotes such as mammals, tens to hundreds of thousands of naturally caused DNA damages occur per cell per day (see next section). While most of these DNA damages can be repaired, such repair is not 100% efficient. Unrepaired DNA damages accumulate, especially in non-replicating or slowl ...
... 2004). In eukaryotes such as mammals, tens to hundreds of thousands of naturally caused DNA damages occur per cell per day (see next section). While most of these DNA damages can be repaired, such repair is not 100% efficient. Unrepaired DNA damages accumulate, especially in non-replicating or slowl ...
Impact of Sample Type and DNA Isolation Procedure on
... sequencing. Our results suggest that inferred community composition was dependent on ...
... sequencing. Our results suggest that inferred community composition was dependent on ...
Quantum Tunneling in DNA
... DNA (deoxyribonucleic acid) is the molecule that encodes all necessary genetic information for use in living organisms–as the atom is said to be the building block of the universe, DNA can be thought of as the building block, or perhaps more accurately as the blueprint for life. Much research has go ...
... DNA (deoxyribonucleic acid) is the molecule that encodes all necessary genetic information for use in living organisms–as the atom is said to be the building block of the universe, DNA can be thought of as the building block, or perhaps more accurately as the blueprint for life. Much research has go ...
Chapter 22: SV40 and Polyomavirus DNA Replication
... also influenced by the nature and composition of the spacer region between pentanucleotide repeats (DeLucia et al. 1983; Dean et al. 1987; Deb et al. 1987). Large T antigen also binds to single-stranded DNA; this activity may be integral to its ability to unwind DNA at the replication fork (for revi ...
... also influenced by the nature and composition of the spacer region between pentanucleotide repeats (DeLucia et al. 1983; Dean et al. 1987; Deb et al. 1987). Large T antigen also binds to single-stranded DNA; this activity may be integral to its ability to unwind DNA at the replication fork (for revi ...
letters
... is fundamental to cell determination and function1. The essential epigenetic systems involved in heritable repression of gene activity are the Polycomb group (PcG) proteins2,3 and the DNA methylation4,5 systems. Here we show that the corresponding silencing pathways are mechanistically linked. We fi ...
... is fundamental to cell determination and function1. The essential epigenetic systems involved in heritable repression of gene activity are the Polycomb group (PcG) proteins2,3 and the DNA methylation4,5 systems. Here we show that the corresponding silencing pathways are mechanistically linked. We fi ...
Electrophoresis Systems for Nucleic Acids
... When nucleic acid (DNA/RNA) is dissolved in buffer solution, etc. it has a negative charge. When this solution (sample) is added to agarose gel and electrophoresed in buffer solution for a regulated time, the level of migration shown is in accordance with the size of nucleic acid due to the molecula ...
... When nucleic acid (DNA/RNA) is dissolved in buffer solution, etc. it has a negative charge. When this solution (sample) is added to agarose gel and electrophoresed in buffer solution for a regulated time, the level of migration shown is in accordance with the size of nucleic acid due to the molecula ...
Non-homologous end-joining factors of Saccharomyces cerevisiae
... DNA double-strand breaks (DSB) are considered to be a severe form of DNA damage, because if left unrepaired, they can cause a cell death and, if misrepaired, they can lead to genomic instability and, ultimately, the development of cancer in multicellular organisms. The budding yeast Saccharomyces ce ...
... DNA double-strand breaks (DSB) are considered to be a severe form of DNA damage, because if left unrepaired, they can cause a cell death and, if misrepaired, they can lead to genomic instability and, ultimately, the development of cancer in multicellular organisms. The budding yeast Saccharomyces ce ...
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... Griffith’s work. Led by the Canadian biologist Oswald Avery, the scientists wanted to determine which molecule in the heat-killed bacteria was most important for transformation. They reasoned that if they could find this particular molecule, it might reveal the chemical nature of the gene. Avery and h ...
... Griffith’s work. Led by the Canadian biologist Oswald Avery, the scientists wanted to determine which molecule in the heat-killed bacteria was most important for transformation. They reasoned that if they could find this particular molecule, it might reveal the chemical nature of the gene. Avery and h ...
DNA polymerase
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.