Cotranscriptional coupling of splicing factor recruitment and
... is cotranscriptionally spliced. Notably, the topoisomerase I inhibitor camptothecin, which stalls elongating Pol II, increased cotranscriptional splicing factor accumulation and splicing in parallel. This provides direct evidence for a kinetic link between transcription, splicing factor recruitment ...
... is cotranscriptionally spliced. Notably, the topoisomerase I inhibitor camptothecin, which stalls elongating Pol II, increased cotranscriptional splicing factor accumulation and splicing in parallel. This provides direct evidence for a kinetic link between transcription, splicing factor recruitment ...
Trawling DNA Databases for Partial Matches: What is the FBI Afraid
... skeptical of such extreme claims. Keith Devlin, a mathematician at Stanford University, calls them "total nonsense" and a "damned lie."' 2 In Devlin's view, it is "disgraceful" that courts allow experts to provide such small random-match probabilities: "They may as well admit al3 chemy and astrology ...
... skeptical of such extreme claims. Keith Devlin, a mathematician at Stanford University, calls them "total nonsense" and a "damned lie."' 2 In Devlin's view, it is "disgraceful" that courts allow experts to provide such small random-match probabilities: "They may as well admit al3 chemy and astrology ...
Identification of Specific DNA Binding Residues in the
... vitro (Kosugi and Ohashi, 2002). These motifs have been identified as cis-elements in many plant genes coding for proteins such as CYCLIN (Li et al., 2005), PCNA (Kosugi and Ohashi, 1997), RADIALIS (Costa et al., 2005), LIPOXIGENASE2 (Schommer et al., 2008), CIRCADIAN ASSOCIATED1 (PrunedaPaz et al., ...
... vitro (Kosugi and Ohashi, 2002). These motifs have been identified as cis-elements in many plant genes coding for proteins such as CYCLIN (Li et al., 2005), PCNA (Kosugi and Ohashi, 1997), RADIALIS (Costa et al., 2005), LIPOXIGENASE2 (Schommer et al., 2008), CIRCADIAN ASSOCIATED1 (PrunedaPaz et al., ...
PDF version - EpiGeneSys
... saturation, and thus chromatin fibre compaction. However, this method consumes a large amount of material, and thus is often not practical.(comment 7) In some circumstances, a thorough analysis of protein content may be necessary ? see (Huynh et al, 2005). This may be the case when working with unus ...
... saturation, and thus chromatin fibre compaction. However, this method consumes a large amount of material, and thus is often not practical.(comment 7) In some circumstances, a thorough analysis of protein content may be necessary ? see (Huynh et al, 2005). This may be the case when working with unus ...
Rapid and Quantitative Detection of Toxoplasma Gondii by PCR
... PCR has greatly improved and simplified the “home brew” applications for prenatal diagnosis of toxoplasmosis, in particular, making it faster, more sensitive, and decreases morbidity, because it is currently based on amniocentesis alone [4]. However, the main risk concerns false-positive results ari ...
... PCR has greatly improved and simplified the “home brew” applications for prenatal diagnosis of toxoplasmosis, in particular, making it faster, more sensitive, and decreases morbidity, because it is currently based on amniocentesis alone [4]. However, the main risk concerns false-positive results ari ...
Cell-Specific Organization of the 5S Ribosomal RNA Gene Cluster
... The 5S rRNA gene cluster was localized as a single focus in fully condensed sperm nuclei with dimensions of less than 1 plm 2 (Fig. c). It should be noted that the 5S rDNA does not occupy the same position in each nucleus. A more detailed examination of the position of this gene in the fully condens ...
... The 5S rRNA gene cluster was localized as a single focus in fully condensed sperm nuclei with dimensions of less than 1 plm 2 (Fig. c). It should be noted that the 5S rDNA does not occupy the same position in each nucleus. A more detailed examination of the position of this gene in the fully condens ...
Epigenetic Mechanisms of Gene Regulation
... associated with transcriptional regulation and higher order chromatin structure. In mammals, DNA methylation patterns are not randomly distributed throughout the genome, but rather methylated DNA is localized to discrete regions of the genome enriched in repetitive DNA and transposable elements, imp ...
... associated with transcriptional regulation and higher order chromatin structure. In mammals, DNA methylation patterns are not randomly distributed throughout the genome, but rather methylated DNA is localized to discrete regions of the genome enriched in repetitive DNA and transposable elements, imp ...
The Mechanism of Insertion of a Segment of
... employed to determine the map position of cloned fragments of unknown origin (Haldenwang et al., 1980; Wilson et al., 1981). Thirdly, an insertion vector has been used to clone one DNA sequence repeatedly from different strains of the same organism (Mkjean et al., 1981). This represents an important ...
... employed to determine the map position of cloned fragments of unknown origin (Haldenwang et al., 1980; Wilson et al., 1981). Thirdly, an insertion vector has been used to clone one DNA sequence repeatedly from different strains of the same organism (Mkjean et al., 1981). This represents an important ...
Revisiting Plus-Strand DNA Synthesis in Retroviruses and Long
... center of HIV-1 RT to alternative sites of hydrolysis. Although the precise mechanism by which this unusual cleavage occurs requires further analysis, we propose that it reflected increased affinity of RT for nucleic acid at the sites of isostere insertion. A protein motif most likely to mediate thi ...
... center of HIV-1 RT to alternative sites of hydrolysis. Although the precise mechanism by which this unusual cleavage occurs requires further analysis, we propose that it reflected increased affinity of RT for nucleic acid at the sites of isostere insertion. A protein motif most likely to mediate thi ...
Diversity of DNA methyltransferases that recognize asymmetric
... DNA methyltransferases (MTases) are a group of enzymes that catalyze the methyl group transfer from S-adenosyl-L-methionine in a sequence-specific manner. Orthodox Type II DNA MTases usually recognize palindromic DNA sequences and add a methyl group to the target base (either adenine or cytosine) on ...
... DNA methyltransferases (MTases) are a group of enzymes that catalyze the methyl group transfer from S-adenosyl-L-methionine in a sequence-specific manner. Orthodox Type II DNA MTases usually recognize palindromic DNA sequences and add a methyl group to the target base (either adenine or cytosine) on ...
The Pif1 family in prokaryotes: what are our helicases doing in your
... Maizels, 2001), not all bacterial genomes the Pif1 family signature sequence are shown (protein sequences were aligned as in Figure 1). Residues that are completely conserved in all six sequences are bold. Residues that are identical encode RecQ homologues (Table 1). It is therefore plausible that p ...
... Maizels, 2001), not all bacterial genomes the Pif1 family signature sequence are shown (protein sequences were aligned as in Figure 1). Residues that are completely conserved in all six sequences are bold. Residues that are identical encode RecQ homologues (Table 1). It is therefore plausible that p ...
Prevention of DNA Rereplication Through a Meiotic Recombination
... ABSTRACT In the budding yeast Saccharomyces cerevisiae, unnatural stabilization of the cyclin-dependent kinase inhibitor Sic1 during meiosis can trigger extra rounds of DNA replication. When programmed DNA double-strand breaks are generated but not repaired due to absence of DMC1, a pathway inv ...
... ABSTRACT In the budding yeast Saccharomyces cerevisiae, unnatural stabilization of the cyclin-dependent kinase inhibitor Sic1 during meiosis can trigger extra rounds of DNA replication. When programmed DNA double-strand breaks are generated but not repaired due to absence of DMC1, a pathway inv ...
Geminivirus Replication Origins Have a Modular
... on the A component encode viral proteins involved in replication. The only viral protein essential for replication is AL1 (Elmer et al., 1988; Hayes and Buck, 1989; Hanley-Bowdoin et al., 1990), but AL3 is necessary for efficient accumulation of viral DNA in plants (Elmer et al., 1988) and in protop ...
... on the A component encode viral proteins involved in replication. The only viral protein essential for replication is AL1 (Elmer et al., 1988; Hayes and Buck, 1989; Hanley-Bowdoin et al., 1990), but AL3 is necessary for efficient accumulation of viral DNA in plants (Elmer et al., 1988) and in protop ...
Propeller-twisted Adenin.Thymine and Guanine.Cytosine Base Pairs
... where e,, e} are partial atomic charges, c is dielectric permitivity, r u are distances between atoms i and j , each of which belongs to one base in the pair. A,}, BtJ and Ci}, D,j are constants describing van der Waals and hydrogen bonding interactions, respectively. The base bond lengths and angle ...
... where e,, e} are partial atomic charges, c is dielectric permitivity, r u are distances between atoms i and j , each of which belongs to one base in the pair. A,}, BtJ and Ci}, D,j are constants describing van der Waals and hydrogen bonding interactions, respectively. The base bond lengths and angle ...
Raven/Johnson Biology 8e Chapter 17 Answers 1. A recombinant
... c. primers to allow PCR amplification of the region between the primers d. a nested set of DNA fragments that end with known bases The correct answer is d— A. Answer a is incorrect. Restriction enzymes cut specific sites but not frequently enough to allow sequencing. ...
... c. primers to allow PCR amplification of the region between the primers d. a nested set of DNA fragments that end with known bases The correct answer is d— A. Answer a is incorrect. Restriction enzymes cut specific sites but not frequently enough to allow sequencing. ...
Detection of Agrobacterium vitis by polymerase chain reaction in
... Although we have not found a primer pair which can detect all pathogenic A. vitis, for routine use the PGF/PGR primers can be used since most (11 out of 14) colonies found in bleeding sap were pathogenic. For large scale application of PCR it is necessary to have a simple rapid protocol to obtain te ...
... Although we have not found a primer pair which can detect all pathogenic A. vitis, for routine use the PGF/PGR primers can be used since most (11 out of 14) colonies found in bleeding sap were pathogenic. For large scale application of PCR it is necessary to have a simple rapid protocol to obtain te ...
Two DNA sites for MelR in the same orientation are sufficient for
... MelR target site 2, whilst in the 227 base pair TB23 fragment, MelR target site 2 is deleted. Results illustrated in Fig. 1c show that, as expected, the deletion of site 2 in the TB23 fragment causes a clear reduction in MelRdependent repression of the melR promoter and confirms previous observation ...
... MelR target site 2, whilst in the 227 base pair TB23 fragment, MelR target site 2 is deleted. Results illustrated in Fig. 1c show that, as expected, the deletion of site 2 in the TB23 fragment causes a clear reduction in MelRdependent repression of the melR promoter and confirms previous observation ...
Chapter 12 Recombinant DNA Technology Key Concepts
... polylinker or multiple cloning site, which contains many unique restriction target sites useful for inserting donor fragments. The polylinker is in frame translationally with the β-galactosidase fragment and does not interfere with its translation. The transformation protocol uses recipient cells th ...
... polylinker or multiple cloning site, which contains many unique restriction target sites useful for inserting donor fragments. The polylinker is in frame translationally with the β-galactosidase fragment and does not interfere with its translation. The transformation protocol uses recipient cells th ...
20.4 RNA polymerase I has a bipartite promoter
... Initiation of transcription requires the enzyme RNA polymerase and transcription factors. Any protein that is needed for the initiation of transcription, but which is not itself part of RNA polymerase, is defined as a transcription factor. Many transcription factors act by recognizing cis-acting sit ...
... Initiation of transcription requires the enzyme RNA polymerase and transcription factors. Any protein that is needed for the initiation of transcription, but which is not itself part of RNA polymerase, is defined as a transcription factor. Many transcription factors act by recognizing cis-acting sit ...
Real-time Quantification of HER2/neu Gene Amplification by
... The LightCycler-CK20 Quantification Kit is specifically adapted for PCR in glass capillaries using the LightCycler Instrument. Detection of cytokeratin 20 (CK20) RNA is conducted by a two-step procedure. In the first step, cDNA is reverse-transcribed from RNA using AMV reverse transcriptase and rand ...
... The LightCycler-CK20 Quantification Kit is specifically adapted for PCR in glass capillaries using the LightCycler Instrument. Detection of cytokeratin 20 (CK20) RNA is conducted by a two-step procedure. In the first step, cDNA is reverse-transcribed from RNA using AMV reverse transcriptase and rand ...
Molecular Design of Unnatural Base
... the shapes of Z and F mimic those of A and T, respectively (Figure 4).11-13 For the Z base, the 1- and 3-nitrogens in A were replaced with carbons, and the 6-amino group was replaced with a methyl group. For the F base, the 3-imino group in T was replaced with a C-H group, and the 2- and 4-keto grou ...
... the shapes of Z and F mimic those of A and T, respectively (Figure 4).11-13 For the Z base, the 1- and 3-nitrogens in A were replaced with carbons, and the 6-amino group was replaced with a methyl group. For the F base, the 3-imino group in T was replaced with a C-H group, and the 2- and 4-keto grou ...
S4O3 Pretest 2015-2016
... 64. Imagine that you are a geneticist working with a couple who are expecting a child. The mother is 41 and the father’s brother has cystic fibrosis. Please describe the risks and benefits of performing amniocentesis and give them information that will help them make a decision as to whether or not ...
... 64. Imagine that you are a geneticist working with a couple who are expecting a child. The mother is 41 and the father’s brother has cystic fibrosis. Please describe the risks and benefits of performing amniocentesis and give them information that will help them make a decision as to whether or not ...
DNA polymerase
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.