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Fig1 from Nature Rev Mol. Cell Biol (Nov2003) 4(11):865
Fig1 from Nature Rev Mol. Cell Biol (Nov2003) 4(11):865

BIOCHEMISTRY 461 Dr. Bourque Chapter 28 Study Questions Fall
BIOCHEMISTRY 461 Dr. Bourque Chapter 28 Study Questions Fall

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... 16.  The  seven  stages,  which  break  down  the  events  of  DNA  replication,  are  listed  below:     i. The  enzyme  gyrase  relieves  any  tension  from  the  unwinding  double  helix.     ii. The  enzyme  helicase  breaks  the ...
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... bacteria also contains an omega subunit, but omega is not involved in RNA polymerization). A single RNA pol found in archeae, but the enzyme has a more complex structure: 8-10 subunits, more similar in its sequence to eukaryotic RNA pol Enzyme in Bacteria has 2 states: Core RNA pol with 2’ and Ho ...
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... Time to put together the two sides. Using the toothpicks and marshmallows, you are to add the nitrogen bases to the sides of the ladder. Remember, the nitrogen bases “come off” the sugar part of the nucleotide. As a result, they should be coming off of the red twizzler. ...
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... 23. The DNA strand C GA T G C G A C A T T undergoes a mutation in which the section coding for the amino acid threonine is lost. Which of the following would be the correct codons after this mutation? a) A C G C U G U AA b) G C U A C G C UG c) G C U C U G U AA d) G C U A C G U AA 24. Give an explana ...
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Plasmid w/ kanamycin resistance (pKAN)
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No Slide Title
No Slide Title

...  There are already more than 1200 type II enzymes isolated from prokaryotic organism  They recognize more than 130 different nucleotide sequence  They scan a DNA molecule, stopping only when it recognizes a specific sequence of nucleotides that are composed of symetrical, palindromic sequence Pal ...
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DNA polymerase



The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.
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