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Profile Documents Logout
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Document
Document

...  Elements of complementary nature spontaneously “stick” together.  This “complementary-attraction-principle” seems to pervade many aspects of life (both molecular and higher levels). ...
Document
Document

... DNA strand separation but do not catalyze the strand separation process. They bind to single strand DNA as soon as it forms and coat it so that it cannot anneal to reform a double helix. Topoisomerases: introduce transient single or double stranded breaks into DNA and thereby allow it to change its ...
Recombinant DNA technology article
Recombinant DNA technology article

... Recombinant DNA technology is another major DNA-based tool that has gained popular attention in the past decade. This technology allows scientists to find individual genes, cut them out, and insert them into the genome of another organism. Recombinant DNA technology has applications in health and nu ...
Chapter 9 Answers to Even Numbered Study Questions
Chapter 9 Answers to Even Numbered Study Questions

... each of the two daughter origins). Thus there are six replication forks active when the cell is born. The first round of replication terminates at five minutes, leaving four active replication forks. The cell now has two separate chromosomes, each of which is about half replicated, so there are now ...
Second Strand cDNA Synthesis Kit
Second Strand cDNA Synthesis Kit

... abm’s Second Strand cDNA Synthesis Kit is an efficient system of generating double stranded cDNA from first strand cDNA templates. The E. coli RNase H nicks RNA in the DNA:RNA hybrid, while the E. coli DNA Polymerase replaces the RNA with deoxyribonucleotides. The E. coli DNA Ligase completes the do ...
Recombinant DNA Simulation
Recombinant DNA Simulation

... Introduction: One of the most important processes developed by biotechnologists was the procedure where a gene is removed from the DNA of one organism and inserted into the DNA of another organism. This technique is called Recombinant DNA. The entire procedure is dependent upon using the correct res ...
DNA - Images
DNA - Images

... These are specific nucleotide sequences encoded in the DNA strands that act as “starting points”. The enzyme helicase unwinds the DNA double helix to create a Replication Bubble (This provides “space” to do the actual building work of making the new complimentary side of the new DNA molecule by othe ...
411-4 OUTLINE I. Spontaneous mutation A. Single base pair
411-4 OUTLINE I. Spontaneous mutation A. Single base pair

... Limitation to fingerprinting system: single base pair changes only detected= Surprise- only 10% of spontaneous changes are base pair changes! OH Frameshifts- one incredible hotspot- ca 2/3 of all mutations ...
Biotechnology:
Biotechnology:

... • Samples of digested DNA are placed in the wells • Electrical leads are attached to the ends of the box creating an electrical potential across the apparatus. • Because DNA has a negative electrical charge. It is "pulled" towards the positive side of the apparatus. • Also, since the smaller molecul ...
Something`s Fishy
Something`s Fishy

... You have learned that DNA is a linear sequence of nucleotides made up of adenine, thymine, guanine, and cytosine. This sequence of A, T, G, and C is unique to each individual. Restriction enzymes cut DNA. Each restriction enzyme recognizes a specific group of “target” base pairs and makes a cut with ...
transcription, translation
transcription, translation

... molecule to be easily transcribed. Whys is this important for genetic information? 3. Whys is RNA important to the cell? How does an mRNA molecule carry information from DNA? 4. If DNA strand read AAC GTC GCG TAC, what would the mRNA strand be? ...
DNA - Southgate Schools
DNA - Southgate Schools

... Nucleotides are joined together to form the DNA chain by links between – A.) deoxyribose molecules and phosphate groups. – B.) adenine and thymine nitrogenous bases. – C.) phosphate groups and guanine nitrogenous bases ...
Restriction Enzymes, Gel Electrophoresis and Mapping DNA
Restriction Enzymes, Gel Electrophoresis and Mapping DNA

Use the following additional information to - biology-with
Use the following additional information to - biology-with

DNA Extraction from Strawberry
DNA Extraction from Strawberry

What does DNA do?
What does DNA do?

... ___ 3) Follow the base pair rule to fill in the missing base pairs for each strand. ___ 4) Compare your strands. Are they similar? What have you done? Where did this process take place in the cell? Why is this process important? They are identical copies of each other (mirror images). This process t ...
Powerpoint Slides 6.1 Part B
Powerpoint Slides 6.1 Part B

... Host Cell Proliferation  Inactivates host p53 (to by pass G1 arrest and prevent apoptosis).  Inactivates the pRB family of proteins (to release E2F transactivator).  stimulates resting cells to move thru the cell cycle and replicate DNA. Viral Gene Expression  Represses its own synthesis  Activ ...
DNA Replication
DNA Replication

...  But they MUST DIVIDE A SECOND TIME to end up with 23 chromosomes in each cell (sperm or egg) created ...
EOC Review Packet #2
EOC Review Packet #2

... 5. What is the process that allows DNA to make copies of itself before cell division? REPLICATION 6. Where in the cell does this take place? THE NUCLEUS 7. Write the Complement of this sequence : ATTCGTTCAG TAAGCAAGTC ...
DNA
DNA

Biology B Final Review ANSWERS
Biology B Final Review ANSWERS

... Describe the structure of DNA proposed by Watson and Crick. The structure was a double helix that twists as it goes Apply Chargaff ’s rule to decide how many guanine bases a length of DNA will have if it has 26 cytosine bases. Explain. It would 26 guanine bases since A=G ...
DNA
DNA

... helping to maximize the odds that each molecule is a perfect copy of the original DNA DNA ligase – connects together the Okazaki fragments on the lagging strand ...
Cloning
Cloning

...  Cut a DNA vector at a unique restriction site and ligate into it the DNA that you want to make a library out of.  If you want a library of human genomic DNA, you use fragmented human DNA.  The ligation mix is not yet considered the library. The library comes after the generation of E. coli cells ...
Individual Identification
Individual Identification

Individual Identification
Individual Identification

... Only semen were collected from both murder scenes. ...
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DNA polymerase



The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.
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