Recombinant DNA Technology
... – Which will carry fragments of DNA into a host cell – Vector DNA functions to insert and amplify the DNA of intersite. • Vectors should contain an origin of replication – Enables the vector, together with the foreign DNA fragment inserted into it, to replicate • they contain one or more single (uni ...
... – Which will carry fragments of DNA into a host cell – Vector DNA functions to insert and amplify the DNA of intersite. • Vectors should contain an origin of replication – Enables the vector, together with the foreign DNA fragment inserted into it, to replicate • they contain one or more single (uni ...
Mitochondrial DNA
... The primers attach to complementary sequences on each half of the open target sequence. These primers then attract the polymerase, which binds to the 3’ end of each primer and proceeds to create a complementary strand to each of the two template strands in the 5’ to 3’ direction. Only DNA containing ...
... The primers attach to complementary sequences on each half of the open target sequence. These primers then attract the polymerase, which binds to the 3’ end of each primer and proceeds to create a complementary strand to each of the two template strands in the 5’ to 3’ direction. Only DNA containing ...
PDF sample
... I will at all costs avoid turning this book into a steaming pile of vocabulary lessons. I will use the authentic science terms when I feel it’s valuable, but I’ll gloss over them or use synonyms when I feel that it’s not. This may anger some of my scientist brethren, but to them I say, “Pffft. Whate ...
... I will at all costs avoid turning this book into a steaming pile of vocabulary lessons. I will use the authentic science terms when I feel it’s valuable, but I’ll gloss over them or use synonyms when I feel that it’s not. This may anger some of my scientist brethren, but to them I say, “Pffft. Whate ...
Clinical Cytogenomics Laboratory
... neutral aberrations. CytoScan HD Array has greater than 99 percent sensitivity and can reliably detect 25-50 kb copy number changes across the genome. With 2.67 million copy number markers (akin to performing 2.7 million simultaneous FISH experiments, including 750000 SNP’s, the Beaumont CytoScan Ar ...
... neutral aberrations. CytoScan HD Array has greater than 99 percent sensitivity and can reliably detect 25-50 kb copy number changes across the genome. With 2.67 million copy number markers (akin to performing 2.7 million simultaneous FISH experiments, including 750000 SNP’s, the Beaumont CytoScan Ar ...
Human Chromosomes - Speedway High School
... In females, nondisjunction can lead to Turner’s syndrome. A female with Turner’s syndrome usually inherits only one X chromosome (karyotype 45,X). Women with Turner’s syndrome are sterile. ...
... In females, nondisjunction can lead to Turner’s syndrome. A female with Turner’s syndrome usually inherits only one X chromosome (karyotype 45,X). Women with Turner’s syndrome are sterile. ...
PlantDirectTM Multiplex PCR System
... 10 second increments, or increasing the extension time in 1minute increments. It is recommended to change one parameter each time. ...
... 10 second increments, or increasing the extension time in 1minute increments. It is recommended to change one parameter each time. ...
techniques in molecular biology – methods
... Most methods start with a large number of bacterial cells, which contain the plasmid of choice and centrifuging down to a pellet. The cells are then lysed by a mixture of the detergent sodium dodecylsulfate (SDS) in basic conditions or by adding a protease (lysozyme) to weaken and disrupt the host c ...
... Most methods start with a large number of bacterial cells, which contain the plasmid of choice and centrifuging down to a pellet. The cells are then lysed by a mixture of the detergent sodium dodecylsulfate (SDS) in basic conditions or by adding a protease (lysozyme) to weaken and disrupt the host c ...
Leukaemia Section t(11;19)(q23;p13.1) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Note: Two different translocations (and two clinical entities), both involving 11q23 with a common breakpoint in MLL, and 19p13 with different breakpoints are now identified: the above mentioned, and the t(11;19)(q23;p13.3). ...
... Note: Two different translocations (and two clinical entities), both involving 11q23 with a common breakpoint in MLL, and 19p13 with different breakpoints are now identified: the above mentioned, and the t(11;19)(q23;p13.3). ...
Title Heterochromatin Blocks Constituting the Entire
... Genomic DNA was extracted from white blood cells, or cultured cells originating from epithelial tissue, using a standard method that included cell lysis with sodium dodecyl sulphate, protein digestion with proteinase K, salt sedimentation, and DNA precipitation with isopropanol. The DNA precipitate ...
... Genomic DNA was extracted from white blood cells, or cultured cells originating from epithelial tissue, using a standard method that included cell lysis with sodium dodecyl sulphate, protein digestion with proteinase K, salt sedimentation, and DNA precipitation with isopropanol. The DNA precipitate ...
Repression of E-cadherin by the Polycomb Group Protein
... promoter. ChIP was carried out using antibodies against HDAC1 and IgG control using DU145 cell line. Addition of 500nM SAHA curtails the recruitment of HDAC1 to the Ecadherin promoter. (d) Ectopically expressed EZH2 recruits PRC2 complex proteins to the E-cadherin promoter. ChIP was carried out usin ...
... promoter. ChIP was carried out using antibodies against HDAC1 and IgG control using DU145 cell line. Addition of 500nM SAHA curtails the recruitment of HDAC1 to the Ecadherin promoter. (d) Ectopically expressed EZH2 recruits PRC2 complex proteins to the E-cadherin promoter. ChIP was carried out usin ...
DNA Markers: Explanation of Validation and Utilization
... discovery population or research herd where cattle have been measured for a number of traits of interest. These animals are extensively genotyped using a large number of markers and then studies are performed to see if there is any association between alleles at these markers and phenotypes for trai ...
... discovery population or research herd where cattle have been measured for a number of traits of interest. These animals are extensively genotyped using a large number of markers and then studies are performed to see if there is any association between alleles at these markers and phenotypes for trai ...
qPCR DNA Extraction and Inhibition Control
... • The optimized control doesn’t match with any sequence routinely found in a lab • The optimized control is detected using a Yakima-Yellow® (VIC® equivalent)-labelled probe • Avoid amplification of endogenous genes. As Sample Processing Control, a given quantity of control DNA is spiked into samples ...
... • The optimized control doesn’t match with any sequence routinely found in a lab • The optimized control is detected using a Yakima-Yellow® (VIC® equivalent)-labelled probe • Avoid amplification of endogenous genes. As Sample Processing Control, a given quantity of control DNA is spiked into samples ...
Whole genome sequencing and assembly of an avian genome, the
... put together into a single continuous sequence of DNA using a computer program called “Genome assembler”. The genome assembler looks for overlapping regions between the sequenced fragments and makes use of this information to place the different fragments with respect to each other. This method of g ...
... put together into a single continuous sequence of DNA using a computer program called “Genome assembler”. The genome assembler looks for overlapping regions between the sequenced fragments and makes use of this information to place the different fragments with respect to each other. This method of g ...
The Art and Science of PCR
... where the primers will sit down are indicated in blue. Note that one primer is the complement of ...
... where the primers will sit down are indicated in blue. Note that one primer is the complement of ...
Chapter 3: Presentation Slides
... which are non-identical but share some genes • Males are genetically haploid for most genes on the X chromosome which results in unique pattern of X-linked inheritance • Autosomes = non-sex chromosomes ...
... which are non-identical but share some genes • Males are genetically haploid for most genes on the X chromosome which results in unique pattern of X-linked inheritance • Autosomes = non-sex chromosomes ...
3rd Lecture
... strand breaks, and DNA-protein cross-links N7 of G is the most nucleophilic site in DNA, at which many ultimate carcinogens form covalent adducts ...
... strand breaks, and DNA-protein cross-links N7 of G is the most nucleophilic site in DNA, at which many ultimate carcinogens form covalent adducts ...
Chromosomes and DNA Replication
... strand is oriented in the 5' to 3' direction while the other strand is oriented in the 3' to 5' direction, Figure 6.26. DNA replication, however, is inflexible: the enzyme that carries out the replication, DNA polymerase, only functions in the 5' to 3' direction. This characteristic of DNA polymeras ...
... strand is oriented in the 5' to 3' direction while the other strand is oriented in the 3' to 5' direction, Figure 6.26. DNA replication, however, is inflexible: the enzyme that carries out the replication, DNA polymerase, only functions in the 5' to 3' direction. This characteristic of DNA polymeras ...
Laboratory 9: Plasmid Isolation
... 1. growth of the bacterial culture, 2. harvesting and lysis of the bacteria, 3. purification of the plasmid DNA Growth of the Bacterial Culture Plasmids should be purified from bacterial cultures that have been inoculated with a single transformed colony picked from an agar plate. At all times, the ...
... 1. growth of the bacterial culture, 2. harvesting and lysis of the bacteria, 3. purification of the plasmid DNA Growth of the Bacterial Culture Plasmids should be purified from bacterial cultures that have been inoculated with a single transformed colony picked from an agar plate. At all times, the ...
Biochemistry
... Nucleotides have a variety of roles in cellular metabolism. They are the energy currency in metabolic transactions, the essential chemical links in the response of cells to hormones and other extracellular stimuli, and the structural components of an array of enzyme cofactors and metabolic intermedi ...
... Nucleotides have a variety of roles in cellular metabolism. They are the energy currency in metabolic transactions, the essential chemical links in the response of cells to hormones and other extracellular stimuli, and the structural components of an array of enzyme cofactors and metabolic intermedi ...
Screening for Recombinants
... Introduction Now that you’ve transformed your DNA and allowed the colonies to grow overnight, you need to determine if they contain the insert of interest. You can either screen them by colony PCR or the more traditional plasmid miniprep followed by restriction digestion. Colony PCR is the most rapi ...
... Introduction Now that you’ve transformed your DNA and allowed the colonies to grow overnight, you need to determine if they contain the insert of interest. You can either screen them by colony PCR or the more traditional plasmid miniprep followed by restriction digestion. Colony PCR is the most rapi ...
LiMA overview
... LiMA – advantages compared to direct detection of bacterial genomes by PCR • LiMA is generic – all bacteria tested contain NAD-dependent DNA ligase. It is difficult to ensure that direct PCR is generic. • LiMA is more sensitive than direct PCR. LiMA involves lysis of the bacilli and release of many ...
... LiMA – advantages compared to direct detection of bacterial genomes by PCR • LiMA is generic – all bacteria tested contain NAD-dependent DNA ligase. It is difficult to ensure that direct PCR is generic. • LiMA is more sensitive than direct PCR. LiMA involves lysis of the bacilli and release of many ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.