Chapter 6: Cell Growth and Reproduction Lesson 6.2
... strand is oriented in the 5' to 3' direction while the other strand is oriented in the 3' to 5' direction, Figure 6.26. DNA replication, however, is inflexible: the enzyme that carries out the replication, DNA polymerase, only functions in the 5' to 3' direction. This characteristic of DNA polymeras ...
... strand is oriented in the 5' to 3' direction while the other strand is oriented in the 3' to 5' direction, Figure 6.26. DNA replication, however, is inflexible: the enzyme that carries out the replication, DNA polymerase, only functions in the 5' to 3' direction. This characteristic of DNA polymeras ...
Applicability and sensitivity of PCR SSCP method for milk species
... techniques. Food Control, 18, 1246-1249. AMILLS, M., FRANCINO, O., JANSA, M. & SÁNCHEZ, A. (1997): Isolation of genomic DNA from milk samples by using Chelex resin. Journal of Dairy Research, 64, 231-238. COMMISSION REGULATION (2006): The protection of geographical indications and designations of or ...
... techniques. Food Control, 18, 1246-1249. AMILLS, M., FRANCINO, O., JANSA, M. & SÁNCHEZ, A. (1997): Isolation of genomic DNA from milk samples by using Chelex resin. Journal of Dairy Research, 64, 231-238. COMMISSION REGULATION (2006): The protection of geographical indications and designations of or ...
Recombinant DNA Technology
... in tandem: TGTGTGTGTGTG is 6 tandem repeats of TG. SSRs are found in and near many genes throughout the genome--they are quite common and easy to find. During normal replication of the DNA in the nucleus, DNA polymerase sometimes slips and creates extra copies or deletes a few copies of the repeat. ...
... in tandem: TGTGTGTGTGTG is 6 tandem repeats of TG. SSRs are found in and near many genes throughout the genome--they are quite common and easy to find. During normal replication of the DNA in the nucleus, DNA polymerase sometimes slips and creates extra copies or deletes a few copies of the repeat. ...
Activity #5b. Plasmid DNA Isolation, Restriction Enzyme Digestion
... endA+ strains such as the JM series, HB101 and its derivatives, or any wild-type strain, which have high levels of nuclease activity or high carbohydrate content. Host strains such as XL-1 Blue and DH5 do not require this additional wash step.) C11. Wash QIAprep Spin Column by adding 0.75 ml Buffer ...
... endA+ strains such as the JM series, HB101 and its derivatives, or any wild-type strain, which have high levels of nuclease activity or high carbohydrate content. Host strains such as XL-1 Blue and DH5 do not require this additional wash step.) C11. Wash QIAprep Spin Column by adding 0.75 ml Buffer ...
Rapid DNA Extraction from Plant Seeds for PCR
... protective devices are a myriad of compounds, some very complex in structure, that allow plants to repel pests.1 These protective compounds include polyphenols and tannins, sometimes found in seeds and seed coats. The presence of storage carbohydrates and polyphenols can interfere with successful am ...
... protective devices are a myriad of compounds, some very complex in structure, that allow plants to repel pests.1 These protective compounds include polyphenols and tannins, sometimes found in seeds and seed coats. The presence of storage carbohydrates and polyphenols can interfere with successful am ...
Human Herpes Virus 8 (Kaposi Sarcoma)
... contains a positive control template. This can be used to generate a standard curve of HHV8 copy number / CT value. Alternatively the positive control can be used at a single dilution where full quantitative analysis of the samples is not required. Each time the kit is used, at least one positive co ...
... contains a positive control template. This can be used to generate a standard curve of HHV8 copy number / CT value. Alternatively the positive control can be used at a single dilution where full quantitative analysis of the samples is not required. Each time the kit is used, at least one positive co ...
Shotgun DNA sequencing using cloned DNase I
... fragments produced by a given enzyme will not yield sequences that meaningfully overlap, and because restriction enzyme sites are usually distributed non-uniformly in complex ...
... fragments produced by a given enzyme will not yield sequences that meaningfully overlap, and because restriction enzyme sites are usually distributed non-uniformly in complex ...
- Department of Molecular Biology and Genetics, Faculty
... Publication of genome sequences permit the design of ‘short’ DNA oligonucleotides (25 70 nts) that are sequencfe specific for particular genes. These single stranded oligos can then by spotted onto the microarray slide and hybridised in ‘ two colour competitive hybridisations’ Oligo probes ...
... Publication of genome sequences permit the design of ‘short’ DNA oligonucleotides (25 70 nts) that are sequencfe specific for particular genes. These single stranded oligos can then by spotted onto the microarray slide and hybridised in ‘ two colour competitive hybridisations’ Oligo probes ...
genetic recombination-unit-2-study material- 2012
... plasmid) in vivo: (1) a general, homologous recombination; (2) a site.specific recombination; and (3) a non-homologous recombination. 1. General homologous recombination. Homologous recombination comprises the mechanism by which the DNA that has been transferred into the recipient cell recombine wit ...
... plasmid) in vivo: (1) a general, homologous recombination; (2) a site.specific recombination; and (3) a non-homologous recombination. 1. General homologous recombination. Homologous recombination comprises the mechanism by which the DNA that has been transferred into the recipient cell recombine wit ...
Cytogenetic and AZF microdeletions on the Y chromosome of
... Intervals V and VI of Yq11.23 regions contain responsible genes for spermatogenesis, and are named as “azoospermia factor locus” (AZF). Deletions of these genes are thought to be pathogenetically involved in some cases of male infertility associated with azoospermia or oligozoospermia. The aim of th ...
... Intervals V and VI of Yq11.23 regions contain responsible genes for spermatogenesis, and are named as “azoospermia factor locus” (AZF). Deletions of these genes are thought to be pathogenetically involved in some cases of male infertility associated with azoospermia or oligozoospermia. The aim of th ...
Taq DNA Polymerase
... Start the PCR program. Once the thermal cycler has reached 94°C, place the PCR tubes in the thermal cycler. In many cases, this simplified hot start improves the specificity of the PCR. Note: After amplification, samples can be stored overnight at 2–8°C, or at –20°C for longer storage. 7. PCR produc ...
... Start the PCR program. Once the thermal cycler has reached 94°C, place the PCR tubes in the thermal cycler. In many cases, this simplified hot start improves the specificity of the PCR. Note: After amplification, samples can be stored overnight at 2–8°C, or at –20°C for longer storage. 7. PCR produc ...
Lab_6_Part3
... You are about to calculate the transformation efficiency, which gives you an indication of how effective you were in getting DNA molecules into bacterial cells. Transformation efficiency is a number. It represents the total number of bacterial cells that express the green protein, divided by the amo ...
... You are about to calculate the transformation efficiency, which gives you an indication of how effective you were in getting DNA molecules into bacterial cells. Transformation efficiency is a number. It represents the total number of bacterial cells that express the green protein, divided by the amo ...
Sample newsletter January 2017
... It is also possible to separate proteins using a special type of agarose, but in contrast to the procedure using polyacryamide, with agarose the proteins are separated by electrical charge only (not charge and size). This is because the pores within the agarose gel are relatively large and the prote ...
... It is also possible to separate proteins using a special type of agarose, but in contrast to the procedure using polyacryamide, with agarose the proteins are separated by electrical charge only (not charge and size). This is because the pores within the agarose gel are relatively large and the prote ...
Karyotype Lab information
... 6. Pair #23 = the Gender Determining Chromosomes (Sex Chromosomes) *may or may not match exactly. *referred to as the X sex chromosome and the Y sex chromosome. *The Y sex chromosome carries the Sex Determining Gene (SRY gene) which is the master switch to the male gender. ...
... 6. Pair #23 = the Gender Determining Chromosomes (Sex Chromosomes) *may or may not match exactly. *referred to as the X sex chromosome and the Y sex chromosome. *The Y sex chromosome carries the Sex Determining Gene (SRY gene) which is the master switch to the male gender. ...
3.3 How Do You Identify and Clone a Gene of Interest?
... • Samples are separated on a single-lane capillary gel that is scanned with a laser beam • Creates different color patterns for each nucleotide • Converted by computer to the sequence ...
... • Samples are separated on a single-lane capillary gel that is scanned with a laser beam • Creates different color patterns for each nucleotide • Converted by computer to the sequence ...
keeSeek: searching distant non-existing words in genomes for PCR
... K-mers generation: Here we report the example and test of 20-mers. Because the number of different k-mers of length l that can be generated using four symbols is 4l, the amount of 20-mers that must be tested when looking for the most distant candidate compared with a reference genome is in the order ...
... K-mers generation: Here we report the example and test of 20-mers. Because the number of different k-mers of length l that can be generated using four symbols is 4l, the amount of 20-mers that must be tested when looking for the most distant candidate compared with a reference genome is in the order ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.