keeSeek: searching distant non-existing words in genomes for PCR
... K-mers generation: Here we report the example and test of 20-mers. Because the number of different k-mers of length l that can be generated using four symbols is 4l, the amount of 20-mers that must be tested when looking for the most distant candidate compared with a reference genome is in the order ...
... K-mers generation: Here we report the example and test of 20-mers. Because the number of different k-mers of length l that can be generated using four symbols is 4l, the amount of 20-mers that must be tested when looking for the most distant candidate compared with a reference genome is in the order ...
GenomeCompress: A Novel Algorithm for DNA
... translated to proteins.[1] Proteins play a mojor role in regulating all the biological functions. It is well-known that DNA sequences, especially in higher eukaryotes, contain many tandem repeats; and also segments that produce noncoding RNA molecules like tRNA, rRNA. Genome may contain several copi ...
... translated to proteins.[1] Proteins play a mojor role in regulating all the biological functions. It is well-known that DNA sequences, especially in higher eukaryotes, contain many tandem repeats; and also segments that produce noncoding RNA molecules like tRNA, rRNA. Genome may contain several copi ...
Rec.DNA.BCH 446,31-32
... – Which will carry fragments of DNA into a host cell – Vector DNA functions to insert and amplify the DNA of intersit . • Vectors should contain an origin of replication – Enables the vector, together with the foreign DNA fragment inserted into it, to replicate • they contain one or more single (uni ...
... – Which will carry fragments of DNA into a host cell – Vector DNA functions to insert and amplify the DNA of intersit . • Vectors should contain an origin of replication – Enables the vector, together with the foreign DNA fragment inserted into it, to replicate • they contain one or more single (uni ...
Information. How to bring your samples
... (qPCR)—is one of the most powerful and sensitive gene analysis techniques available. It is used for a broad range of applications including quantitative gene expression analysis, genotyping, copy number, drug target validation, biomarker discovery, pathogen detection, and measuring RNA interference. ...
... (qPCR)—is one of the most powerful and sensitive gene analysis techniques available. It is used for a broad range of applications including quantitative gene expression analysis, genotyping, copy number, drug target validation, biomarker discovery, pathogen detection, and measuring RNA interference. ...
Meiosis
... • Ensures that the next generation will have a combination of traits from both parents ...
... • Ensures that the next generation will have a combination of traits from both parents ...
Genetic Mapping with CAPS Markers
... However, markers for genetic mapping don’t necessarily have to be mutations that cause phenotypic changes. They can also be variations in DNA sequences that are detectable by molecular methods. In Arabidopsis thaliana, molecular markers exploit the natural differences between distinct ecotypes (sub- ...
... However, markers for genetic mapping don’t necessarily have to be mutations that cause phenotypic changes. They can also be variations in DNA sequences that are detectable by molecular methods. In Arabidopsis thaliana, molecular markers exploit the natural differences between distinct ecotypes (sub- ...
Nucleic acids and chromosomes
... condenses to form two sister chromatids held together at the centromere. Describe the Human Karyotype Somatic cells are diploid and have 2 copies of each chromosome, 23 pairs of chromosome, contain 22 pairs of autosome and 1 pair of sex chromosomes In a standard karyotype the chromosomes are disting ...
... condenses to form two sister chromatids held together at the centromere. Describe the Human Karyotype Somatic cells are diploid and have 2 copies of each chromosome, 23 pairs of chromosome, contain 22 pairs of autosome and 1 pair of sex chromosomes In a standard karyotype the chromosomes are disting ...
File
... This DNA fingerprint can then be used to compare against other fingerprints (possible suspects, DNA found at a crime scene or family member DNA). Prior to 1993, these tests had limitations - they required LARGE amounts of DNA (not what you would see on CSI). ...
... This DNA fingerprint can then be used to compare against other fingerprints (possible suspects, DNA found at a crime scene or family member DNA). Prior to 1993, these tests had limitations - they required LARGE amounts of DNA (not what you would see on CSI). ...
CHAPTER 8 Recombinant DNA Technology
... 1. Screening the genomic library of an organism with a large genome is laborious. Screening time can be reduced if a gene has been localized to a chromosome, by examining a library made from only that chromosome. Human, for example, have 24 different chromosome libraries (22 autosomes, X and Y). 2. ...
... 1. Screening the genomic library of an organism with a large genome is laborious. Screening time can be reduced if a gene has been localized to a chromosome, by examining a library made from only that chromosome. Human, for example, have 24 different chromosome libraries (22 autosomes, X and Y). 2. ...
Analysis by pulsed-field gel electrophoresis mutations in the
... expression of this phenotype. Five such regulatory mutations were analysed in detail by PFGE and DNA hybridisation and were shown to be located at five different chromosomal loci, although three of the five loci were located on the same 330-kb SmaI fragment of the wild-type strain Eagan chromosome. ...
... expression of this phenotype. Five such regulatory mutations were analysed in detail by PFGE and DNA hybridisation and were shown to be located at five different chromosomal loci, although three of the five loci were located on the same 330-kb SmaI fragment of the wild-type strain Eagan chromosome. ...
Essential Cell Biology chapter 5 excerpt
... DNA and genes have come from experiments in a wide variety of organisms. We then consider how genes and other important segments of DNA are arranged in the long molecules of DNA that are present in the chromosomes of cells. Finally, we discuss how eucaryotic cells fold these long DNA molecules into ...
... DNA and genes have come from experiments in a wide variety of organisms. We then consider how genes and other important segments of DNA are arranged in the long molecules of DNA that are present in the chromosomes of cells. Finally, we discuss how eucaryotic cells fold these long DNA molecules into ...
MyTaq™ Blood PCR Kit
... simultaneously during the 1st step of gene amplification followed by the 2nd step of analyte-specific primer extension of 29 different primers without additional MyTaq enzyme; (4) amplify detectable quantities of all amplicons despite the variability in number of buccal cells collected from patient ...
... simultaneously during the 1st step of gene amplification followed by the 2nd step of analyte-specific primer extension of 29 different primers without additional MyTaq enzyme; (4) amplify detectable quantities of all amplicons despite the variability in number of buccal cells collected from patient ...
Identification of disease genes by whole genome
... oligonucleotide microarray analysis (29,30) and single nucleotide polymorphism oligonucleotide arrays (SNP arrays) (31). When compared with conventional karyotyping, array CGH provides a higher resolution, a higher dynamic range and better possibilities for automation. In addition, it allows for dir ...
... oligonucleotide microarray analysis (29,30) and single nucleotide polymorphism oligonucleotide arrays (SNP arrays) (31). When compared with conventional karyotyping, array CGH provides a higher resolution, a higher dynamic range and better possibilities for automation. In addition, it allows for dir ...
Improvement of DNA Extraction Protocols for Nostochopsis spp.
... DNA (Figure 4C). Bead-beating is used as the extraction method of nucleic acids from a wide variety of organisms for which lysis can otherwise be difficult [20]. Sample materials became more disrupted when liquid nitrogen was used in conjunction with bead mill. However, if the disruption is prolonge ...
... DNA (Figure 4C). Bead-beating is used as the extraction method of nucleic acids from a wide variety of organisms for which lysis can otherwise be difficult [20]. Sample materials became more disrupted when liquid nitrogen was used in conjunction with bead mill. However, if the disruption is prolonge ...
Solid Tumour Section Nervous system: Medulloblastoma Atlas of Genetics and Cytogenetics
... Classic medulloblastoma composed of densely jacked round-cells with round to oval hyperchromatic nuclei. Desmoplastic medulloblastoma represents a variant with abundant reticulin and collagen. Large cell medulloblastoma is a rare variant composed of cells with large round nuclei. ...
... Classic medulloblastoma composed of densely jacked round-cells with round to oval hyperchromatic nuclei. Desmoplastic medulloblastoma represents a variant with abundant reticulin and collagen. Large cell medulloblastoma is a rare variant composed of cells with large round nuclei. ...
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS)
... to the offspring, while in other cases they can hamper meiosis up to the arrest of gametogenesis, or may give rise to unbalanced gametes (13,14,15,16,17,18,19). Most diagnosed CCRs are three-way rearrangements, and only a minority consists of highly complex aberrations (20, 21). When the number of b ...
... to the offspring, while in other cases they can hamper meiosis up to the arrest of gametogenesis, or may give rise to unbalanced gametes (13,14,15,16,17,18,19). Most diagnosed CCRs are three-way rearrangements, and only a minority consists of highly complex aberrations (20, 21). When the number of b ...
gen-305-presentation-14-16
... recombinant DNA. In this case, the ends are ‘sticky’ in that they have a short, single-stranded end that can base-pair with another piece of DNA cut with the same enzyme. ...
... recombinant DNA. In this case, the ends are ‘sticky’ in that they have a short, single-stranded end that can base-pair with another piece of DNA cut with the same enzyme. ...
QIAquick® Gel Extraction Kit
... Ordering www.qiagen.com/contact | Technical Support support.qiagen.com | Website www.qiagen.com ...
... Ordering www.qiagen.com/contact | Technical Support support.qiagen.com | Website www.qiagen.com ...
Table 3.1. List of suppliers of restriction enzymes. Name of
... (v) Mix the lysate with phenol. Phenol and water is separated into layers. Phenol layer contains the contaminating proteins and RNAase, and water based lysate layer contains plasmid DNA. (vi) Remove the phenol layer. Precipitate the water based layer with alcohol as the genomic DNA is removed. Make ...
... (v) Mix the lysate with phenol. Phenol and water is separated into layers. Phenol layer contains the contaminating proteins and RNAase, and water based lysate layer contains plasmid DNA. (vi) Remove the phenol layer. Precipitate the water based layer with alcohol as the genomic DNA is removed. Make ...
Fatma El-Sayed Ibrahim Ali_A Symmetric Encryption Algorithm
... messages with various sizes to test the proposed scheme, the estimated storage size in Kilobytes. The experiments are conducted using Intel(R) Core(TM) i5-2430M CPU, 2.40 GHz,64 bit processor with 4 GB of RAM. The simulation program is compiled using NetBeans 7.1.1 for java windows application under ...
... messages with various sizes to test the proposed scheme, the estimated storage size in Kilobytes. The experiments are conducted using Intel(R) Core(TM) i5-2430M CPU, 2.40 GHz,64 bit processor with 4 GB of RAM. The simulation program is compiled using NetBeans 7.1.1 for java windows application under ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.