GENETIC ENGINEERING QUESTIONS
... BIO H DR WEINER Multiple Choice (6 pts) 1. A scientist wants to mass produce a recombinant form of a protein for human use. He should first a. Purify the protein b. Clone the gene for the protein c. Perform PCR on the protein d. Grow bacteria to make the protein 2. People show restriction fragment l ...
... BIO H DR WEINER Multiple Choice (6 pts) 1. A scientist wants to mass produce a recombinant form of a protein for human use. He should first a. Purify the protein b. Clone the gene for the protein c. Perform PCR on the protein d. Grow bacteria to make the protein 2. People show restriction fragment l ...
Biodosimetry - Arkansas State University
... “high throughput” biodosimetry can, at best, assess a few hundred individuals / day. The Center for High-Throughput Minimally-Invasive Radiation Biodosimetry is a research consortium devoted to developing highthroughput radiation dose assessment ...
... “high throughput” biodosimetry can, at best, assess a few hundred individuals / day. The Center for High-Throughput Minimally-Invasive Radiation Biodosimetry is a research consortium devoted to developing highthroughput radiation dose assessment ...
Comparative Genomic Hybridization in Chronic B
... chromosomal regions can be examined in a single experiment. With the recently developed methodof comparative genomic hybridization (CGH),I6 tumor genomes can be rapidly tested for the presenceof chromosomal imbalances (such as partial or complete monosomies and trisomies).“”* Differentially labeled ...
... chromosomal regions can be examined in a single experiment. With the recently developed methodof comparative genomic hybridization (CGH),I6 tumor genomes can be rapidly tested for the presenceof chromosomal imbalances (such as partial or complete monosomies and trisomies).“”* Differentially labeled ...
Genetic Technology
... Sorts DNA based on size & charge Can combine with specific probes to label particular DNA bands ...
... Sorts DNA based on size & charge Can combine with specific probes to label particular DNA bands ...
biotechnology
... 7. Statement A: During genetically engineered biosynthesis of insulin, A-chain and B-chain are separated from their respective fusion proteins by treatment with carboxypeptidase and trypsin. Statement B: After the separation of A and B chains from fusion proteins, the two chains are bonded by disulp ...
... 7. Statement A: During genetically engineered biosynthesis of insulin, A-chain and B-chain are separated from their respective fusion proteins by treatment with carboxypeptidase and trypsin. Statement B: After the separation of A and B chains from fusion proteins, the two chains are bonded by disulp ...
Transgenic Organisms
... can be used to produce human proteins 2. Animals can be used to improve food supply, or to study effect of human diseases 3. Plants – genes can be implanted that provide plants with natural insecticides, or resistance to various chemicals ...
... can be used to produce human proteins 2. Animals can be used to improve food supply, or to study effect of human diseases 3. Plants – genes can be implanted that provide plants with natural insecticides, or resistance to various chemicals ...
Why the scientists want to extract the DNA from the cells? With the
... Thirdly, the DNA samples can help with the extinction of different kinds of organisms, to maintain the biodiversity. Lots of organisms face extinction, but once their DNA samples store in the database, they still have chances to rebirth again, in another way to keep the world’s biodiveristy. Finally ...
... Thirdly, the DNA samples can help with the extinction of different kinds of organisms, to maintain the biodiversity. Lots of organisms face extinction, but once their DNA samples store in the database, they still have chances to rebirth again, in another way to keep the world’s biodiveristy. Finally ...
Unit 2 Review: Molecular Genetics
... -DNA must be packed tightly to fit in nucleus (1.8m long) -double helix is wrapped around histones to form nucleosomes, which are coiled into chromatin fibres, which are then supercoiled -individuals have microsatellites (random repeats, non-coding) that make them unique -some can cause diseases (Hu ...
... -DNA must be packed tightly to fit in nucleus (1.8m long) -double helix is wrapped around histones to form nucleosomes, which are coiled into chromatin fibres, which are then supercoiled -individuals have microsatellites (random repeats, non-coding) that make them unique -some can cause diseases (Hu ...
DNA-protein interaction
... Chromatin immunoprecipitation coupled with highthroughput sequencing A different way to read out the number of sequence bound by a protein Potentially more accurate because not cross-hybridization ...
... Chromatin immunoprecipitation coupled with highthroughput sequencing A different way to read out the number of sequence bound by a protein Potentially more accurate because not cross-hybridization ...
Biology 303 EXAM II 3/14/00 NAME
... During replication of DNA, strand elongation proceeds A. in a 5' to 3' direction on the leading strand, but in a 3' to 5' direction on the lagging strand. B. in a 3' to 5' direction on the leading strand, but in a 5' to 3' direction on the lagging strand. C. in a 5' to 3' direction on both the leadi ...
... During replication of DNA, strand elongation proceeds A. in a 5' to 3' direction on the leading strand, but in a 3' to 5' direction on the lagging strand. B. in a 3' to 5' direction on the leading strand, but in a 5' to 3' direction on the lagging strand. C. in a 5' to 3' direction on both the leadi ...
Biology 303 EXAM II 3/14/00 NAME
... Which of the following clusters of terms accurately describes DNA as it is generally viewed to exist in prokaryotes and eukaryotes? A. Double-stranded, parallel, (A+T)/(C+G)= variable, (A+G)/(C+T)=1.0 B. Single-stranded, antiparallel, (A+T)/(C+G)=1.0, (A+G)/C+T)=1.0 C. Double-stranded, antiparallel, ...
... Which of the following clusters of terms accurately describes DNA as it is generally viewed to exist in prokaryotes and eukaryotes? A. Double-stranded, parallel, (A+T)/(C+G)= variable, (A+G)/(C+T)=1.0 B. Single-stranded, antiparallel, (A+T)/(C+G)=1.0, (A+G)/C+T)=1.0 C. Double-stranded, antiparallel, ...
CST Review
... functions of DNA, RNA, and protein. BI1. d. Students know the central dogma of molecular biology outlines the flow of information from transcription of ribonucleic acid (RNA) in the nucleus to translation of proteins on ribosomes in the cytoplasm. BI2. a. Students know meiosis is an early step in se ...
... functions of DNA, RNA, and protein. BI1. d. Students know the central dogma of molecular biology outlines the flow of information from transcription of ribonucleic acid (RNA) in the nucleus to translation of proteins on ribosomes in the cytoplasm. BI2. a. Students know meiosis is an early step in se ...
lecture2
... the -OH at the 3′ carbon atom (red arrow). A dideoxynucleotide (dideoxythymidine triphosphate — ddTTP — is the one shown here) can be added to the growing DNA strand but when it is, chain elongation stops because there is no 3′ -OH for the next nucleotide to be attached to. For this reason, the dide ...
... the -OH at the 3′ carbon atom (red arrow). A dideoxynucleotide (dideoxythymidine triphosphate — ddTTP — is the one shown here) can be added to the growing DNA strand but when it is, chain elongation stops because there is no 3′ -OH for the next nucleotide to be attached to. For this reason, the dide ...
Chapter 5
... Chapter 5 Part III. Recombinant DNA technology • Cloning strategies • Polymerase chain reaction (PCR) • Applications ...
... Chapter 5 Part III. Recombinant DNA technology • Cloning strategies • Polymerase chain reaction (PCR) • Applications ...
E coli
... • Bacterial chromosome is a large (4 Mb in E coli) circular molecule • Bacterial cells may also contain small circular chromosomes called plasmids (4kb - 100kb; 1 - 1000 copies) that code for optional functions such as antibiotic resistance • Will look at circular DNA in this lecture • The bacterial ...
... • Bacterial chromosome is a large (4 Mb in E coli) circular molecule • Bacterial cells may also contain small circular chromosomes called plasmids (4kb - 100kb; 1 - 1000 copies) that code for optional functions such as antibiotic resistance • Will look at circular DNA in this lecture • The bacterial ...
9.1 Manipulating DNA
... • Restriction enzymes act as “molecular scissors.” – come from various types of bacteria – allow scientists to more easily study and manipulate genes – cut DNA at a specific nucleotide sequence called a restriction site ...
... • Restriction enzymes act as “molecular scissors.” – come from various types of bacteria – allow scientists to more easily study and manipulate genes – cut DNA at a specific nucleotide sequence called a restriction site ...
9.1 Manipulating DNA KEY CONCEPT Biotechnology relies on cutting DNA at specific places.
... • Restriction enzymes act as “molecular scissors.” – come from various types of bacteria – allow scientists to more easily study and manipulate genes – cut DNA at a specific nucleotide sequence called a restriction site ...
... • Restriction enzymes act as “molecular scissors.” – come from various types of bacteria – allow scientists to more easily study and manipulate genes – cut DNA at a specific nucleotide sequence called a restriction site ...
The first midterm will consist of 20 four
... 7) A SNP is an example of a) a frame shift mutation b) transpositional control c) genetic regulation d) a genetic marker 8) The gene defect for both Huntington's Disease and Fragile-X syndrome consists of a) a series of repeated nucleotide sequences b) a mispairing of base pairs c) a major ...
... 7) A SNP is an example of a) a frame shift mutation b) transpositional control c) genetic regulation d) a genetic marker 8) The gene defect for both Huntington's Disease and Fragile-X syndrome consists of a) a series of repeated nucleotide sequences b) a mispairing of base pairs c) a major ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.