Name:
... 21. What would be an example of incomplete dominance? 22. How many chromosomes does a human have in each cell? _____ ...
... 21. What would be an example of incomplete dominance? 22. How many chromosomes does a human have in each cell? _____ ...
Trends in Biotechnology
... Sensitivity of Southern blots have been increased by: 1.Increased activity of labeled probes. 2.Development of nonradioactive (color, fluorescent, or light-producing) probes. 3.Treatments to prevent nonspecific binding of probes to membranes. 4.Use of detectors (phosphoimagers) to obtain images of ...
... Sensitivity of Southern blots have been increased by: 1.Increased activity of labeled probes. 2.Development of nonradioactive (color, fluorescent, or light-producing) probes. 3.Treatments to prevent nonspecific binding of probes to membranes. 4.Use of detectors (phosphoimagers) to obtain images of ...
DNA REPLICATION HANDOUT
... 1) Template strands: Original DNA strands that were ripped apart. 2) Replication Fork: Y-shaped region where new strands of DNA are elongated 3) Okazaki Fragments: Only found on the lagging strand. Since DNA is connected by base pairs, as the original strand “unzips” one of the templates is running ...
... 1) Template strands: Original DNA strands that were ripped apart. 2) Replication Fork: Y-shaped region where new strands of DNA are elongated 3) Okazaki Fragments: Only found on the lagging strand. Since DNA is connected by base pairs, as the original strand “unzips” one of the templates is running ...
Name
... Cut DNA with restriction enzymes and run samples through gel electrophoresis Size, smaller fragments will migrate further/faster than larger fragments Restriction site Restriction fragments/DNA fingerprints and no two people (except identical twins) have the same DNA Amplify a small portion of DNA ...
... Cut DNA with restriction enzymes and run samples through gel electrophoresis Size, smaller fragments will migrate further/faster than larger fragments Restriction site Restriction fragments/DNA fingerprints and no two people (except identical twins) have the same DNA Amplify a small portion of DNA ...
Quick DNA Extraction from Rice Seed (Wet)
... assay block. Incubate the samples in about 1” of water at 95ºC for 20 minutes then place them on ice for approximately 10 minutes or until samples are cool to the touch. Centrifuge again for 1 minute. Add neutralizing extraction buffer and seal the assay block with sealing film. Centrifuge the sampl ...
... assay block. Incubate the samples in about 1” of water at 95ºC for 20 minutes then place them on ice for approximately 10 minutes or until samples are cool to the touch. Centrifuge again for 1 minute. Add neutralizing extraction buffer and seal the assay block with sealing film. Centrifuge the sampl ...
Genetic Engineering
... 1.Create recombinant bacteria with desired gene. 2. Allow the bacteria to “infect" the plant cells. ...
... 1.Create recombinant bacteria with desired gene. 2. Allow the bacteria to “infect" the plant cells. ...
Chapter 13
... Clone: a collection of molecules or cells, all identical to an original molecule or cell • To "clone a gene" is to make many copies of it - for example, in a population of bacteria • Gene can be an exact copy of a natural gene • Gene can be an altered version of a natural gene • Recombinant DNA tech ...
... Clone: a collection of molecules or cells, all identical to an original molecule or cell • To "clone a gene" is to make many copies of it - for example, in a population of bacteria • Gene can be an exact copy of a natural gene • Gene can be an altered version of a natural gene • Recombinant DNA tech ...
36_sequencing
... Find the clones that contain coding sequences • Make a DNA copy (“cDNA”) of the mRNA using Reverse Transcriptase • Use that to probe for clones that contain coding sequences ...
... Find the clones that contain coding sequences • Make a DNA copy (“cDNA”) of the mRNA using Reverse Transcriptase • Use that to probe for clones that contain coding sequences ...
File - MRS. WILSON Science
... of replication. The double helix unzips in both directions along the strand. Eukaryotic chromosomes are very long, so they have many origins of replication to help speed the process. Other proteins hold the two strands apart. • The unzipping exposes the bases on the DNA strands and enables free-floa ...
... of replication. The double helix unzips in both directions along the strand. Eukaryotic chromosomes are very long, so they have many origins of replication to help speed the process. Other proteins hold the two strands apart. • The unzipping exposes the bases on the DNA strands and enables free-floa ...
Y13 IB Biology Revision
... The other day I met a genetic engineer. His latest project is the mixing DNA from different species of birds. First he combined the DNA from a pheasant and a hen. It worked! He called it a "Phen." Next he successfully combined a pheasant and a goose. He called it a "Phoose” Yesterday, he was able to ...
... The other day I met a genetic engineer. His latest project is the mixing DNA from different species of birds. First he combined the DNA from a pheasant and a hen. It worked! He called it a "Phen." Next he successfully combined a pheasant and a goose. He called it a "Phoose” Yesterday, he was able to ...
Chapter Objectives: Chapter 20 Biotechnology
... 1. Explain how advances in recombinant DNA technology have helped scientists study the eukaryotic genome 2. Describe the natural function of restriction enzymes 3. Describe how restriction enzymes and gel electrophoresis are used to isolate DNA fragments 4. Explain how the creation of sticky ends by ...
... 1. Explain how advances in recombinant DNA technology have helped scientists study the eukaryotic genome 2. Describe the natural function of restriction enzymes 3. Describe how restriction enzymes and gel electrophoresis are used to isolate DNA fragments 4. Explain how the creation of sticky ends by ...
Genetic Engineering
... 1.Create recombinant bacteria with desired gene. 2. Allow the bacteria to “infect" the plant cells. ...
... 1.Create recombinant bacteria with desired gene. 2. Allow the bacteria to “infect" the plant cells. ...
DNA quantification
... • Concentration and quality of a sample of DNA or RNA are measured with a UV spectrophotometer. • Since nitrogenous bases absorb UV light, the more concentrated the DNA solution, the more UV light it will absorb. • A solution containing 50 µg per ml of double strand DNA has an absorbancy (optical de ...
... • Concentration and quality of a sample of DNA or RNA are measured with a UV spectrophotometer. • Since nitrogenous bases absorb UV light, the more concentrated the DNA solution, the more UV light it will absorb. • A solution containing 50 µg per ml of double strand DNA has an absorbancy (optical de ...
Chem*4570 Applied Biochemistry Lecture 11 Conjugation and
... to another cell. Recombination events may then integrate the genes into the recipient’s genome. F plasmid may also itself become incorporated into the genome, in which case tranfer of the F factor to the recipient can carry the entire genome into the recipient. Since this creates far more opportunit ...
... to another cell. Recombination events may then integrate the genes into the recipient’s genome. F plasmid may also itself become incorporated into the genome, in which case tranfer of the F factor to the recipient can carry the entire genome into the recipient. Since this creates far more opportunit ...
Lctures Clinical genetics – 4
... Chk the presence or absence of seq, copy number and chromosomal location of seq at least several kb long. or presence or absence of ...
... Chk the presence or absence of seq, copy number and chromosomal location of seq at least several kb long. or presence or absence of ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.