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How hair can reveal a history
How hair can reveal a history

... get one copy of each chromosome from our mother and one from our father, there are two numbers of repeats for each locus, which appear as peaks on an electropherogram, a chart produced by a genetic analyzer. The chance that two people have the same pairs at all 13 loci is astronomically low. It’s a ...
SNC2D Genes - Malvern Science
SNC2D Genes - Malvern Science

... • Humans have 46 chromosomes in each somatic (body) cell (except the gametes = ovum/sperm, which have 23) • Different species may have different numbers of chromosomes ...
forensics_by_students
forensics_by_students

... examination of DNA sequences. To identify individuals, 13 DNA regions are scanned. Each region varies from person to person. The unique data provided by an individual is used to create a DNA profile which is also known as their fingerprint. There is an extremely small chance that another person has ...
LECTURE 16 – Using Genomic Variation for Identity DNA Level
LECTURE 16 – Using Genomic Variation for Identity DNA Level

... Ø Plasmids – vectors that carry genes in bacteria, they are self replicating circles of DNA Ø Antibiotic resistance – select for a recombinant DNA molecule Ø Restriction enzymes – cut DNA molecules at specific sequence Ø DNA ligase – join DNA fragments together Ø Transformation – uptake of exogenous ...
Genetics - Liberty Public Schools
Genetics - Liberty Public Schools

Gene Cloning and Karyotyping
Gene Cloning and Karyotyping

... (PCR) clones DNA entirely in vitro • When the source of DNA is scanty or impure, the polymerase chain reaction (PCR) is quicker and more selective. Its limitation is that PCR only produces short DNA segments within a gene and not entire genes. • This technique can quickly amplify any piece of DNA wi ...
DNA Structure and Lab
DNA Structure and Lab

... ____________________ (C) The Genetic Code (p. 132) DNA makes up _____________. Genes control _____________________________________________________________________ The order of _________________ bases along a gene forms a __________________ code that specifies what type of _______________ will be pro ...
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Fifth Lecture

... • DNA is the most important material making up the chromosomes. • It determines what types of RNA are produced which, in turn, determine the types of protein that are produced. • The DNA molecule takes the form of a twisted ladder or double helix. • The sides of the ladder are strands of alternating ...
the nucleic acids - Y11-Biology-SG
the nucleic acids - Y11-Biology-SG

... Bases on opposite strands pair up according to a rule: A pairs up with T C pairs up with G This simple rule always applies in nucleic acids and it is key for replication and transmission of genetic information to the next generation. 3 weak H–bonds ...
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... • Each strand must untwist and unzip. Controlled by enzymes known as DNA helicases. ...
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... • General Scheme: Extract mRNA, synthesize labeled cDNA, Hybridize with DNA on the array, – DNA Chips (Affymetrix) and MicroArrays can measure mRNA concentration of thousands of genes simultaneously, ...
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... Why are additudes about GMO’s different around the world? 90% of people that live in North America trust the USDA, whereas in Britain the regulatory agencies are treated with suspicion. There is good reason for this, considering outbreaks such as the mad cow disease. ...
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DNA Structure and Replication

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Discovery of DNA structure

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A History of Innovation in Genetic Analysis

... The first breast cancer gene, BRCA1, is discovered. ...
Review Sheet NYS Regents Lab Activity #1 Relationships and Biodiversity
Review Sheet NYS Regents Lab Activity #1 Relationships and Biodiversity

... pigments blue, yellow, and pink, scattered bundles, no difference in the amino acid sequences, and the same DNA banding pattern. 4. The evidence that should receive the most emphasis when determining the relatedness would be the genetic sequence, as many things can look similar structurally (converg ...
genetic engineering
genetic engineering

... First, the DNA is extracted/ removed from an opened cell. Restriction enzymes are then used to cut the DNA at a specific section of nucleotides. The fragments are then separated and analyzed using gel electrophoresis (used to compare genomes of different organisms)  this way scientists can locate & ...
Notes: Introduction to Single Nucleotide Polymorphisms (SNPs)
Notes: Introduction to Single Nucleotide Polymorphisms (SNPs)

... organisms who are closely related to us, with the idea that their genomes were not likely to suffer the same mutation. Chimpanzee DNA is the obvious choice, since chimps are closer in DNA sequence to humans than any other living species. Finding sequences in the chimp genome similar to human SNPs ma ...
understanding dna molecule of heredity - Cal State LA
understanding dna molecule of heredity - Cal State LA

PreAP Biology Study Guide Unit 4: Molecular Genetics 4.1 What are
PreAP Biology Study Guide Unit 4: Molecular Genetics 4.1 What are

... In 1952, Alfred Hershey and Martha Chase conducted an experiment to conclusively prove that DNA, and not proteins, were the macromolecules that were passed on to the next generation and actually contained the “information” for creating a organism. This experiment which involved the radioactive eleme ...
DNA structure and replication Three key features needed for any
DNA structure and replication Three key features needed for any

... 1) Must allow for faithful replication - each strand of DNA serves as a template for replication 2) Must have information content - the sequence of bases predict the sequence of amino acids in proteins 3) Must be able to change in order to explain mutations changes in DNA sequences result in changes ...
The Masterof
The Masterof

Biology 303 EXAM II 3/14/00 NAME
Biology 303 EXAM II 3/14/00 NAME

Genetics 3500 winter Test ii_ansers
Genetics 3500 winter Test ii_ansers

< 1 ... 187 188 189 190 191 192 193 194 195 ... 222 >

Comparative genomic hybridization



Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.
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