Characterization of Proteins Structurally Related to Human N
... recovered from the DEAE-cellulose column (Braidman et al., 1974). When the concentrated fractions were examined by immunodiffusion with antihexosaminidase serum, several precipitin lines were observed (Fig. la). The B-fraction contained at least one major cross-reacting protein in addition to hexosa ...
... recovered from the DEAE-cellulose column (Braidman et al., 1974). When the concentrated fractions were examined by immunodiffusion with antihexosaminidase serum, several precipitin lines were observed (Fig. la). The B-fraction contained at least one major cross-reacting protein in addition to hexosa ...
Dynamic properties of a reconstituted myelin sheath
... of PNS myelin [11], where it colocalizes with another myelin-specific protein, P2. The P2 protein, while missing in CNS, represents 1–15% of the total myelin protein in the PNS. MBP is essential for the formation of CNS myelin, able to interact with a wide range of ligands, often polyanionic in natu ...
... of PNS myelin [11], where it colocalizes with another myelin-specific protein, P2. The P2 protein, while missing in CNS, represents 1–15% of the total myelin protein in the PNS. MBP is essential for the formation of CNS myelin, able to interact with a wide range of ligands, often polyanionic in natu ...
Ideal Drug for Blood Pressure
... residues. These residues are involved in recognition of the ligands (drugs). Usually, an enzyme molecule has 10 possible ligand binding sites, out of which only one functions as active site, and the active site fits with one specific type of ligand molecule.12 A tighter fit between an active site an ...
... residues. These residues are involved in recognition of the ligands (drugs). Usually, an enzyme molecule has 10 possible ligand binding sites, out of which only one functions as active site, and the active site fits with one specific type of ligand molecule.12 A tighter fit between an active site an ...
Protein-protein interaction networks (I): data preprocessing
... Figure 3: Source [18]: The Septin complex and its mapping by the different methods . Gray areas are the true members of a septin complex. As can be seen HMS-PCI revealed 5 out of 7 members of the complex, while TAP in addition to stating those same 5 proteins also included 2 false positives. Y2H ind ...
... Figure 3: Source [18]: The Septin complex and its mapping by the different methods . Gray areas are the true members of a septin complex. As can be seen HMS-PCI revealed 5 out of 7 members of the complex, while TAP in addition to stating those same 5 proteins also included 2 false positives. Y2H ind ...
Hsp70 and Hsp90 of E. coli Directly Interact for Collaboration in
... groundwork for the development of new therapeutic strategies targeting multiple chaperones and cochaperones. Published by Elsevier Ltd. ...
... groundwork for the development of new therapeutic strategies targeting multiple chaperones and cochaperones. Published by Elsevier Ltd. ...
Hemoglobin Receptor in Leishmania Is a Hexokinase Located in the
... Determination of Binding and Uptake of 125I-Hb by Leishmania— Hemoglobin binding and uptake by Leishmania were determined as described previously (8). Briefly, Hb was labeled with Na125I by iodine monochloride-catalyzed reaction, and ⬎99% of the radioactivity was acid-precipitable with a specific ac ...
... Determination of Binding and Uptake of 125I-Hb by Leishmania— Hemoglobin binding and uptake by Leishmania were determined as described previously (8). Briefly, Hb was labeled with Na125I by iodine monochloride-catalyzed reaction, and ⬎99% of the radioactivity was acid-precipitable with a specific ac ...
The integrity of a cholesterol-binding pocket in Niemann–Pick C2
... The 3D-PSSM web-based homology server (16, 17) identified the Der p 2 dust mite allergen as an excellent template for modeling the NPC2 protein structure (E value 7.14e-13) based on multiple sequence alignment, secondary structure prediction, and solvation potential. The Der p 2 structure is a  san ...
... The 3D-PSSM web-based homology server (16, 17) identified the Der p 2 dust mite allergen as an excellent template for modeling the NPC2 protein structure (E value 7.14e-13) based on multiple sequence alignment, secondary structure prediction, and solvation potential. The Der p 2 structure is a  san ...
9/12
... Lophotrichous = tuft at one or both ends Peritrichous = spread evenly over the entire surface Arrangement can be useful in identification ...
... Lophotrichous = tuft at one or both ends Peritrichous = spread evenly over the entire surface Arrangement can be useful in identification ...
An intersubunit lock-and-key `Clasp` motif in the dimer interface of
... playing a major role in detoxication mechanisms by nucleophilic addition of GSH (γ -glutamyl-L-cysteinyl-glycine) to the electrophilic centre of diverse non-polar endogenous and xenobiotic compounds, rendering them less reactive and more water-soluble substances [1–4]. Cytosolic GSTs are ubiquitous ...
... playing a major role in detoxication mechanisms by nucleophilic addition of GSH (γ -glutamyl-L-cysteinyl-glycine) to the electrophilic centre of diverse non-polar endogenous and xenobiotic compounds, rendering them less reactive and more water-soluble substances [1–4]. Cytosolic GSTs are ubiquitous ...
Accurate Prediction of Contact Numbers for Multi
... Generation of Data Set. The data set of HMPs with known structures used in the current study was retrieved from the OPM (Orientation of Proteins in the Membrane) database.24 Peripheral HMPs and peptides were removed to obtain a set of “true” HMPs. Further refinement was carried out by removing thylak ...
... Generation of Data Set. The data set of HMPs with known structures used in the current study was retrieved from the OPM (Orientation of Proteins in the Membrane) database.24 Peripheral HMPs and peptides were removed to obtain a set of “true” HMPs. Further refinement was carried out by removing thylak ...
antihistaminic
... of two or three carbon atoms. 3- The terminal nitrogen atom is a simple dimethyl amino moiety. However, it may be a part of a heterocyclic structure as in (piperazine, pyrrolidine , piperidine, imidazole…….). ...
... of two or three carbon atoms. 3- The terminal nitrogen atom is a simple dimethyl amino moiety. However, it may be a part of a heterocyclic structure as in (piperazine, pyrrolidine , piperidine, imidazole…….). ...
Structural Characterization of the GSK
... and neurological diseases such as bipolar disorders or Alzheimer’s disease.10 To date, three groups have published structures of GSK-3b. These include two structures of the unphosphorylated apoenzyme,11,12 one structure of ...
... and neurological diseases such as bipolar disorders or Alzheimer’s disease.10 To date, three groups have published structures of GSK-3b. These include two structures of the unphosphorylated apoenzyme,11,12 one structure of ...
Recent Advances in Target Characterization and Identification by
... available functionalized benzophenone derivatives to the probe. Otherwise, the benzophenone motif can be synthesized from a suitable precursor bearing the desired linking unit. The utilization of benzophenone-derived affinity probes has been widely explored, revealing both its advantages and disadva ...
... available functionalized benzophenone derivatives to the probe. Otherwise, the benzophenone motif can be synthesized from a suitable precursor bearing the desired linking unit. The utilization of benzophenone-derived affinity probes has been widely explored, revealing both its advantages and disadva ...
Site-Directed Mutagenesis of Predicted Active Site Residues in
... study by Robinson et al. (1987). Enzymatic activity of GCP II in 2 mg of total protein from the cell lysate was assayed in a final volume of 250 ml using 30 nM N-acetyl-L-aspartyl-L-[3,4-3H]glutamate in 50 mM Tris, pH 7.4, at 37°C, 0.1% Triton X-100, 150 mM potassium phosphate, and 1 mM CoCl2 (Robin ...
... study by Robinson et al. (1987). Enzymatic activity of GCP II in 2 mg of total protein from the cell lysate was assayed in a final volume of 250 ml using 30 nM N-acetyl-L-aspartyl-L-[3,4-3H]glutamate in 50 mM Tris, pH 7.4, at 37°C, 0.1% Triton X-100, 150 mM potassium phosphate, and 1 mM CoCl2 (Robin ...
The Dihydropyridine-sensitive Calcium Channel of the Skeletal Muscle
... P-subunit The /?-subunit has an apparent molecular mass of 55 kDa. The protein is apparently not glycosylated (Takahashi et al. 1987). The /?-subunit contains several specific phosphorylation sites, which are phosphorylated by cAMPkinase (at Ser 182) and other sites may be phosporylated by protein k ...
... P-subunit The /?-subunit has an apparent molecular mass of 55 kDa. The protein is apparently not glycosylated (Takahashi et al. 1987). The /?-subunit contains several specific phosphorylation sites, which are phosphorylated by cAMPkinase (at Ser 182) and other sites may be phosporylated by protein k ...
Doehrty-McMahon 2009
... endocytosis plays a key role in regulating, continue to expand and include processes as seemingly disparate as mitosis, antigen presentation, and cell migration. Furthermore, it is becoming apparent that endocytosis plays key roles in the positive regulation of many intracellular signaling cascades ...
... endocytosis plays a key role in regulating, continue to expand and include processes as seemingly disparate as mitosis, antigen presentation, and cell migration. Furthermore, it is becoming apparent that endocytosis plays key roles in the positive regulation of many intracellular signaling cascades ...
Direct Demonstration of a Physiological Role for Carbon Monoxide
... Figure 1. Colocalization of HO-2 and soluble guanylyl cyclase in olfactory neuroepithelium. Coronal sections of normal adult olfactory neuroepithelium (A and B) and of adult olfactory epithelium three days post-bulbectomy (C and D) were immunostained with affinity-purified antibodies to HO-2 (A and ...
... Figure 1. Colocalization of HO-2 and soluble guanylyl cyclase in olfactory neuroepithelium. Coronal sections of normal adult olfactory neuroepithelium (A and B) and of adult olfactory epithelium three days post-bulbectomy (C and D) were immunostained with affinity-purified antibodies to HO-2 (A and ...
Protein quality control and elimination of protein waste: The role of
... ATP-independent chaperones. In yeast, the two most prominent members are Hsp42 and Hsp26. Hsp42 functions both in stressed and unstressed cells and prevents protein aggregation [70], but is also involved in targeting of misfolded proteins to peripheral aggregate deposits in cells [71]. Hsp26 is stre ...
... ATP-independent chaperones. In yeast, the two most prominent members are Hsp42 and Hsp26. Hsp42 functions both in stressed and unstressed cells and prevents protein aggregation [70], but is also involved in targeting of misfolded proteins to peripheral aggregate deposits in cells [71]. Hsp26 is stre ...
Interplay of pH and Binding of Multivalent Metal Ions: Charge
... cause overcharging of the surface, attraction between likecharged surfaces, and charge inversion of colloidal particles.30−35 A complete model for the protein charge in any system has to include both local and nonlocal contributions, although many studies focus on only one approach.36,37 The driving ...
... cause overcharging of the surface, attraction between likecharged surfaces, and charge inversion of colloidal particles.30−35 A complete model for the protein charge in any system has to include both local and nonlocal contributions, although many studies focus on only one approach.36,37 The driving ...
AFFINITY OF WARFARIN WITH CYP2C9 BY MOLECULAR DOCKING STUDY Original Article
... the most widely used oral anticoagulant drugs worldwide. They function as antagonists of vitamin K, an essential cofactor for the posttranslational γ-glutamyl carboxylation so called as vitamin Kdependent proteins. [3] The CYP2C9 ranks amongst the most important drug metabolizing enzymes in humans, ...
... the most widely used oral anticoagulant drugs worldwide. They function as antagonists of vitamin K, an essential cofactor for the posttranslational γ-glutamyl carboxylation so called as vitamin Kdependent proteins. [3] The CYP2C9 ranks amongst the most important drug metabolizing enzymes in humans, ...
Tunicates and not cephalochordates are the closest living relatives
... with MP (90%), a method known to be more sensitive to LBA than probabilistic methods14. Indeed, when O. dioica was used as the single representative of tunicates, MP unambiguously supported (BP = 100) an aberrant position for this group which emerged before cnidarians, disrupting the monophyly of bi ...
... with MP (90%), a method known to be more sensitive to LBA than probabilistic methods14. Indeed, when O. dioica was used as the single representative of tunicates, MP unambiguously supported (BP = 100) an aberrant position for this group which emerged before cnidarians, disrupting the monophyly of bi ...
The common northern periwinkle, Littorina littorea
... PKG apparently stimulating a specific PK-kinase that, in turn, phosphorylates and inactivates PK (Brooks and Storey, 1991). The potential for wide-ranging effects by cGMP and PKG in the control of other metabolic responses to anoxia is high but remains an unexplored area. ...
... PKG apparently stimulating a specific PK-kinase that, in turn, phosphorylates and inactivates PK (Brooks and Storey, 1991). The potential for wide-ranging effects by cGMP and PKG in the control of other metabolic responses to anoxia is high but remains an unexplored area. ...
EMBO_only European Molecular Biology Organization European
... al, 2005), suggesting that PI3K and Ras/MAPK pathways collaborate to inhibit TSC1–TSC2 function in response to growth factors. Whereas TSC2 functions as a GAP toward the small Ras-related GTPase Rheb, TSC1 is required to stabilize TSC2 and prevent its proteasomal degradation (Huang and Manning, 2008 ...
... al, 2005), suggesting that PI3K and Ras/MAPK pathways collaborate to inhibit TSC1–TSC2 function in response to growth factors. Whereas TSC2 functions as a GAP toward the small Ras-related GTPase Rheb, TSC1 is required to stabilize TSC2 and prevent its proteasomal degradation (Huang and Manning, 2008 ...
Proteomic analyses of Oryza sativa mature pollen reveal novel
... the ovule by its polarily growing pollen tube. Yet the molecular genetic basis of these functions is poorly understood. Here, we identified 322 unique proteins, most of which were not reported previously to be in pollen, from mature pollen of Oryza sativa L. ssp japonica using a proteomic approach, ...
... the ovule by its polarily growing pollen tube. Yet the molecular genetic basis of these functions is poorly understood. Here, we identified 322 unique proteins, most of which were not reported previously to be in pollen, from mature pollen of Oryza sativa L. ssp japonica using a proteomic approach, ...
Enzymes of the mevalonate pathway of isoprenoid
... target of the family of statin inhibitors of cholesterol biosynthesis. These enzymes had been isolated and classical enzymological and biochemical characterization had been performed prior to the evolution of experimental tools that have expedited the understanding the molecular basis of enzyme func ...
... target of the family of statin inhibitors of cholesterol biosynthesis. These enzymes had been isolated and classical enzymological and biochemical characterization had been performed prior to the evolution of experimental tools that have expedited the understanding the molecular basis of enzyme func ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).